Norwalk virus (strain GI/Human/United States/Norwalk/1968) (Hu/NV/NV/1968/US)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Picornavirales; Caliciviridae; Norovirus; Norwalk virus; Norovirus isolates

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q83885|VP2_NVN68 Protein VP2 OS=Norwalk virus (strain GI/Human/United States/Norwalk/1968) OX=524364 GN=ORF3 PE=3 SV=1
MM1 pKa = 7.14MMASKK6 pKa = 10.62DD7 pKa = 3.49ATSSVDD13 pKa = 3.33GASGAGQLVPEE24 pKa = 4.82VNASDD29 pKa = 4.55PLAMDD34 pKa = 4.29PVAGSSTAVATAGQVNPIDD53 pKa = 3.3PWIINNFVQAPQGEE67 pKa = 4.72FTISPNNTPGDD78 pKa = 3.77VLFDD82 pKa = 4.42LSLGPHH88 pKa = 6.92LNPFLLHH95 pKa = 6.7LSQMYY100 pKa = 10.11NGWVGNMRR108 pKa = 11.84VRR110 pKa = 11.84IMLAGNAFTAGKK122 pKa = 9.71IIVSCIPPGFGSHH135 pKa = 6.39NLTIAQATLFPHH147 pKa = 6.69VIADD151 pKa = 3.52VRR153 pKa = 11.84TLDD156 pKa = 4.47PIEE159 pKa = 4.53VPLEE163 pKa = 3.86DD164 pKa = 3.42VRR166 pKa = 11.84NVLFHH171 pKa = 6.97NNDD174 pKa = 3.29RR175 pKa = 11.84NQQTMRR181 pKa = 11.84LVCMLYY187 pKa = 9.52TPLRR191 pKa = 11.84TGGGTGDD198 pKa = 3.66SFVVAGRR205 pKa = 11.84VMTCPSPDD213 pKa = 3.49FNFLFLVPPTVEE225 pKa = 3.74QKK227 pKa = 8.96TRR229 pKa = 11.84PFTLPNLPLSSLSNSRR245 pKa = 11.84APLPISSMGISPDD258 pKa = 3.29NVQSVQFQNGRR269 pKa = 11.84CTLDD273 pKa = 3.03GRR275 pKa = 11.84LVGTTPVSLSHH286 pKa = 5.38VAKK289 pKa = 10.57IRR291 pKa = 11.84GTSNGTVINLTEE303 pKa = 4.18LDD305 pKa = 3.86GTPFHH310 pKa = 6.94PFEE313 pKa = 5.62GPAPIGFPDD322 pKa = 4.63LGGCDD327 pKa = 2.82WHH329 pKa = 8.91INMTQFGHH337 pKa = 6.56SSQTQYY343 pKa = 11.76DD344 pKa = 3.76VDD346 pKa = 3.97TTPDD350 pKa = 3.15TFVPHH355 pKa = 6.93LGSIQANGIGSGNYY369 pKa = 9.67VGVLSWISPPSHH381 pKa = 6.98PSGSQVDD388 pKa = 3.57LWKK391 pKa = 10.44IPNYY395 pKa = 10.07GSSITEE401 pKa = 4.01ATHH404 pKa = 6.83LAPSVYY410 pKa = 9.74PPGFGEE416 pKa = 4.08VLVFFMSKK424 pKa = 9.24MPGPGAYY431 pKa = 9.94NLPCLLPQEE440 pKa = 4.92YY441 pKa = 9.31ISHH444 pKa = 7.11LASEE448 pKa = 4.39QAPTVGEE455 pKa = 4.1AALLHH460 pKa = 6.16YY461 pKa = 10.43VDD463 pKa = 5.61PDD465 pKa = 3.24TGRR468 pKa = 11.84NLGEE472 pKa = 4.03FKK474 pKa = 10.59AYY476 pKa = 9.47PDD478 pKa = 3.78GFLTCVPNGASSGPQQLPINGVFVFVSWVSRR509 pKa = 11.84FYY511 pKa = 10.66QLKK514 pKa = 9.46PVGTASSARR523 pKa = 11.84GRR525 pKa = 11.84LGLRR529 pKa = 11.84RR530 pKa = 3.73

Molecular weight: 56.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q83885|VP2_NVN68 Protein VP2 OS=Norwalk virus (strain GI/Human/United States/Norwalk/1968) OX=524364 GN=ORF3 PE=3 SV=1
MM1 pKa = 7.14AQAIIGAIAASTAGSALGAGIQVGGEE27 pKa = 3.81AALQSQRR34 pKa = 11.84YY35 pKa = 5.62QQNLQLQEE43 pKa = 4.08NSFKK47 pKa = 10.49HH48 pKa = 5.32DD49 pKa = 3.36RR50 pKa = 11.84EE51 pKa = 4.22MIGYY55 pKa = 8.43QVEE58 pKa = 4.17ASNQLLAKK66 pKa = 10.25NLATRR71 pKa = 11.84YY72 pKa = 9.64SLLRR76 pKa = 11.84AGGLTSADD84 pKa = 3.32AARR87 pKa = 11.84SVAGAPVTRR96 pKa = 11.84IVDD99 pKa = 3.52WNGVRR104 pKa = 11.84VSAPEE109 pKa = 3.87SSATTLRR116 pKa = 11.84SGGFMSVPIPFASKK130 pKa = 9.85QKK132 pKa = 9.84QVQSSGISNPNYY144 pKa = 9.74SPSSISRR151 pKa = 11.84TTSWVEE157 pKa = 3.84SQNSSRR163 pKa = 11.84FGNLSPYY170 pKa = 9.62HH171 pKa = 7.12AEE173 pKa = 3.9ALNTVWLTPPGSTASSTLSSVPRR196 pKa = 11.84GYY198 pKa = 11.02FNTDD202 pKa = 3.16RR203 pKa = 11.84LPLFANNRR211 pKa = 11.84RR212 pKa = 3.65

Molecular weight: 22.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2531

212

1789

843.7

92.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.744 ± 0.986

1.304 ± 0.277

5.334 ± 0.85

5.452 ± 1.459

3.635 ± 0.583

8.376 ± 0.582

2.252 ± 0.279

4.939 ± 0.258

4.82 ± 1.682

8.179 ± 0.359

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.726 ± 0.293

4.188 ± 0.839

6.519 ± 1.321

4.623 ± 0.411

5.255 ± 0.672

7.428 ± 1.766

6.282 ± 0.129

6.875 ± 0.576

1.58 ± 0.201

2.489 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski