Amino acid dipepetide frequency for Mycobacterium phage Bipper

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.53AlaAla: 16.53 ± 1.274
1.008AlaCys: 1.008 ± 0.203
8.628AlaAsp: 8.628 ± 0.62
6.652AlaGlu: 6.652 ± 0.575
2.016AlaPhe: 2.016 ± 0.315
7.983AlaGly: 7.983 ± 0.952
2.016AlaHis: 2.016 ± 0.273
4.556AlaIle: 4.556 ± 0.386
5.201AlaLys: 5.201 ± 0.51
8.063AlaLeu: 8.063 ± 0.56
3.588AlaMet: 3.588 ± 0.394
3.669AlaAsn: 3.669 ± 0.488
4.838AlaPro: 4.838 ± 0.53
5.12AlaGln: 5.12 ± 0.72
7.821AlaArg: 7.821 ± 0.724
5.12AlaSer: 5.12 ± 0.478
6.451AlaThr: 6.451 ± 0.838
7.862AlaVal: 7.862 ± 0.512
1.895AlaTrp: 1.895 ± 0.318
2.741AlaTyr: 2.741 ± 0.312
0.0AlaXaa: 0.0 ± 0.0
Cys
1.048CysAla: 1.048 ± 0.19
0.161CysCys: 0.161 ± 0.079
0.968CysAsp: 0.968 ± 0.219
0.564CysGlu: 0.564 ± 0.186
0.161CysPhe: 0.161 ± 0.073
2.258CysGly: 2.258 ± 0.405
0.363CysHis: 0.363 ± 0.151
0.282CysIle: 0.282 ± 0.095
0.121CysLys: 0.121 ± 0.076
0.685CysLeu: 0.685 ± 0.18
0.161CysMet: 0.161 ± 0.074
0.524CysAsn: 0.524 ± 0.157
1.693CysPro: 1.693 ± 0.325
0.484CysGln: 0.484 ± 0.159
1.25CysArg: 1.25 ± 0.272
0.766CysSer: 0.766 ± 0.172
0.766CysThr: 0.766 ± 0.187
0.806CysVal: 0.806 ± 0.196
0.363CysTrp: 0.363 ± 0.123
0.161CysTyr: 0.161 ± 0.079
0.0CysXaa: 0.0 ± 0.0
Asp
6.088AspAla: 6.088 ± 0.499
0.847AspCys: 0.847 ± 0.199
6.37AspAsp: 6.37 ± 0.753
4.838AspGlu: 4.838 ± 0.491
1.169AspPhe: 1.169 ± 0.267
6.531AspGly: 6.531 ± 0.544
1.411AspHis: 1.411 ± 0.259
2.459AspIle: 2.459 ± 0.31
1.774AspLys: 1.774 ± 0.264
4.878AspLeu: 4.878 ± 0.538
1.371AspMet: 1.371 ± 0.21
2.298AspAsn: 2.298 ± 0.322
4.878AspPro: 4.878 ± 0.448
3.588AspGln: 3.588 ± 0.346
4.999AspArg: 4.999 ± 0.42
3.225AspSer: 3.225 ± 0.446
3.87AspThr: 3.87 ± 0.358
4.394AspVal: 4.394 ± 0.406
1.25AspTrp: 1.25 ± 0.271
1.814AspTyr: 1.814 ± 0.294
0.0AspXaa: 0.0 ± 0.0
Glu
5.483GluAla: 5.483 ± 0.503
0.927GluCys: 0.927 ± 0.207
3.87GluAsp: 3.87 ± 0.375
2.903GluGlu: 2.903 ± 0.441
1.774GluPhe: 1.774 ± 0.243
3.669GluGly: 3.669 ± 0.326
1.129GluHis: 1.129 ± 0.238
1.492GluIle: 1.492 ± 0.238
1.693GluLys: 1.693 ± 0.334
6.289GluLeu: 6.289 ± 0.549
1.411GluMet: 1.411 ± 0.214
1.411GluAsn: 1.411 ± 0.286
3.628GluPro: 3.628 ± 0.386
2.822GluGln: 2.822 ± 0.356
4.274GluArg: 4.274 ± 0.477
3.225GluSer: 3.225 ± 0.356
2.54GluThr: 2.54 ± 0.318
4.757GluVal: 4.757 ± 0.473
1.492GluTrp: 1.492 ± 0.266
1.089GluTyr: 1.089 ± 0.185
0.0GluXaa: 0.0 ± 0.0
Phe
2.459PheAla: 2.459 ± 0.324
0.161PheCys: 0.161 ± 0.082
2.258PheAsp: 2.258 ± 0.309
1.33PheGlu: 1.33 ± 0.232
0.484PhePhe: 0.484 ± 0.151
2.137PheGly: 2.137 ± 0.292
0.645PheHis: 0.645 ± 0.17
0.726PheIle: 0.726 ± 0.154
0.645PheLys: 0.645 ± 0.165
1.371PheLeu: 1.371 ± 0.201
0.605PheMet: 0.605 ± 0.163
0.968PheAsn: 0.968 ± 0.224
1.653PhePro: 1.653 ± 0.281
1.25PheGln: 1.25 ± 0.248
1.935PheArg: 1.935 ± 0.248
1.008PheSer: 1.008 ± 0.188
1.492PheThr: 1.492 ± 0.203
1.653PheVal: 1.653 ± 0.218
0.403PheTrp: 0.403 ± 0.107
0.564PheTyr: 0.564 ± 0.14
0.0PheXaa: 0.0 ± 0.0
Gly
7.257GlyAla: 7.257 ± 0.996
1.411GlyCys: 1.411 ± 0.241
6.047GlyAsp: 6.047 ± 0.543
3.911GlyGlu: 3.911 ± 0.377
2.379GlyPhe: 2.379 ± 0.428
8.789GlyGly: 8.789 ± 1.651
2.903GlyHis: 2.903 ± 0.366
2.822GlyIle: 2.822 ± 0.352
2.661GlyLys: 2.661 ± 0.301
7.378GlyLeu: 7.378 ± 0.55
1.693GlyMet: 1.693 ± 0.267
2.741GlyAsn: 2.741 ± 0.307
4.999GlyPro: 4.999 ± 0.538
4.394GlyGln: 4.394 ± 0.457
5.886GlyArg: 5.886 ± 0.443
4.193GlySer: 4.193 ± 0.435
6.652GlyThr: 6.652 ± 0.589
6.934GlyVal: 6.934 ± 0.522
2.459GlyTrp: 2.459 ± 0.288
2.943GlyTyr: 2.943 ± 0.359
0.0GlyXaa: 0.0 ± 0.0
His
2.58HisAla: 2.58 ± 0.417
0.443HisCys: 0.443 ± 0.133
1.653HisAsp: 1.653 ± 0.297
0.806HisGlu: 0.806 ± 0.161
0.605HisPhe: 0.605 ± 0.184
2.701HisGly: 2.701 ± 0.376
1.25HisHis: 1.25 ± 0.295
0.968HisIle: 0.968 ± 0.208
0.685HisLys: 0.685 ± 0.143
2.298HisLeu: 2.298 ± 0.367
0.564HisMet: 0.564 ± 0.145
0.685HisAsn: 0.685 ± 0.155
2.217HisPro: 2.217 ± 0.311
1.169HisGln: 1.169 ± 0.237
1.975HisArg: 1.975 ± 0.323
0.726HisSer: 0.726 ± 0.153
1.572HisThr: 1.572 ± 0.305
1.371HisVal: 1.371 ± 0.278
0.564HisTrp: 0.564 ± 0.169
0.927HisTyr: 0.927 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
5.604IleAla: 5.604 ± 0.37
0.363IleCys: 0.363 ± 0.115
2.217IleAsp: 2.217 ± 0.272
2.903IleGlu: 2.903 ± 0.329
0.403IlePhe: 0.403 ± 0.176
3.467IleGly: 3.467 ± 0.341
0.847IleHis: 0.847 ± 0.178
0.927IleIle: 0.927 ± 0.203
1.048IleLys: 1.048 ± 0.174
1.653IleLeu: 1.653 ± 0.267
0.564IleMet: 0.564 ± 0.126
1.089IleAsn: 1.089 ± 0.204
2.298IlePro: 2.298 ± 0.291
1.089IleGln: 1.089 ± 0.214
2.741IleArg: 2.741 ± 0.341
1.693IleSer: 1.693 ± 0.215
3.064IleThr: 3.064 ± 0.377
2.54IleVal: 2.54 ± 0.309
0.645IleTrp: 0.645 ± 0.156
0.484IleTyr: 0.484 ± 0.148
0.0IleXaa: 0.0 ± 0.0
Lys
3.991LysAla: 3.991 ± 0.498
0.484LysCys: 0.484 ± 0.135
1.653LysAsp: 1.653 ± 0.242
1.613LysGlu: 1.613 ± 0.217
0.968LysPhe: 0.968 ± 0.252
2.419LysGly: 2.419 ± 0.295
0.766LysHis: 0.766 ± 0.192
1.169LysIle: 1.169 ± 0.211
0.564LysLys: 0.564 ± 0.154
2.379LysLeu: 2.379 ± 0.38
0.847LysMet: 0.847 ± 0.18
0.403LysAsn: 0.403 ± 0.125
2.137LysPro: 2.137 ± 0.294
0.887LysGln: 0.887 ± 0.199
2.903LysArg: 2.903 ± 0.383
1.209LysSer: 1.209 ± 0.267
1.693LysThr: 1.693 ± 0.283
2.983LysVal: 2.983 ± 0.335
0.645LysTrp: 0.645 ± 0.187
0.726LysTyr: 0.726 ± 0.159
0.0LysXaa: 0.0 ± 0.0
Leu
9.716LeuAla: 9.716 ± 0.673
1.048LeuCys: 1.048 ± 0.239
5.483LeuAsp: 5.483 ± 0.384
4.636LeuGlu: 4.636 ± 0.424
2.056LeuPhe: 2.056 ± 0.323
6.975LeuGly: 6.975 ± 0.611
2.137LeuHis: 2.137 ± 0.318
2.782LeuIle: 2.782 ± 0.336
2.54LeuLys: 2.54 ± 0.365
7.015LeuLeu: 7.015 ± 0.636
1.613LeuMet: 1.613 ± 0.24
2.096LeuAsn: 2.096 ± 0.377
4.838LeuPro: 4.838 ± 0.521
2.661LeuGln: 2.661 ± 0.356
4.556LeuArg: 4.556 ± 0.409
3.588LeuSer: 3.588 ± 0.404
6.168LeuThr: 6.168 ± 0.492
5.483LeuVal: 5.483 ± 0.488
1.209LeuTrp: 1.209 ± 0.241
2.016LeuTyr: 2.016 ± 0.291
0.0LeuXaa: 0.0 ± 0.0
Met
2.983MetAla: 2.983 ± 0.307
0.242MetCys: 0.242 ± 0.105
1.048MetAsp: 1.048 ± 0.176
1.048MetGlu: 1.048 ± 0.211
0.443MetPhe: 0.443 ± 0.14
1.774MetGly: 1.774 ± 0.228
0.403MetHis: 0.403 ± 0.137
0.968MetIle: 0.968 ± 0.188
0.726MetLys: 0.726 ± 0.178
1.855MetLeu: 1.855 ± 0.335
0.887MetMet: 0.887 ± 0.18
0.968MetAsn: 0.968 ± 0.192
1.089MetPro: 1.089 ± 0.163
0.484MetGln: 0.484 ± 0.13
1.653MetArg: 1.653 ± 0.252
2.661MetSer: 2.661 ± 0.33
3.064MetThr: 3.064 ± 0.337
1.492MetVal: 1.492 ± 0.25
0.363MetTrp: 0.363 ± 0.12
0.484MetTyr: 0.484 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
3.911AsnAla: 3.911 ± 0.628
0.524AsnCys: 0.524 ± 0.154
1.492AsnAsp: 1.492 ± 0.217
1.814AsnGlu: 1.814 ± 0.35
0.524AsnPhe: 0.524 ± 0.152
3.346AsnGly: 3.346 ± 0.378
1.129AsnHis: 1.129 ± 0.245
0.887AsnIle: 0.887 ± 0.173
0.524AsnLys: 0.524 ± 0.23
2.177AsnLeu: 2.177 ± 0.316
0.605AsnMet: 0.605 ± 0.144
0.806AsnAsn: 0.806 ± 0.224
2.782AsnPro: 2.782 ± 0.333
0.968AsnGln: 0.968 ± 0.322
2.701AsnArg: 2.701 ± 0.347
1.209AsnSer: 1.209 ± 0.245
1.653AsnThr: 1.653 ± 0.244
2.338AsnVal: 2.338 ± 0.275
0.564AsnTrp: 0.564 ± 0.137
0.443AsnTyr: 0.443 ± 0.139
0.0AsnXaa: 0.0 ± 0.0
Pro
6.612ProAla: 6.612 ± 0.491
0.847ProCys: 0.847 ± 0.193
5.241ProAsp: 5.241 ± 0.56
4.032ProGlu: 4.032 ± 0.454
1.532ProPhe: 1.532 ± 0.235
5.402ProGly: 5.402 ± 0.493
1.613ProHis: 1.613 ± 0.316
3.225ProIle: 3.225 ± 0.382
1.855ProLys: 1.855 ± 0.261
4.112ProLeu: 4.112 ± 0.438
1.774ProMet: 1.774 ± 0.247
1.693ProAsn: 1.693 ± 0.244
4.475ProPro: 4.475 ± 0.669
2.338ProGln: 2.338 ± 0.265
3.467ProArg: 3.467 ± 0.514
3.306ProSer: 3.306 ± 0.363
4.596ProThr: 4.596 ± 0.497
5.04ProVal: 5.04 ± 0.503
1.572ProTrp: 1.572 ± 0.234
1.371ProTyr: 1.371 ± 0.209
0.0ProXaa: 0.0 ± 0.0
Gln
4.596GlnAla: 4.596 ± 0.581
0.403GlnCys: 0.403 ± 0.164
1.25GlnAsp: 1.25 ± 0.224
1.532GlnGlu: 1.532 ± 0.221
1.089GlnPhe: 1.089 ± 0.2
3.467GlnGly: 3.467 ± 0.422
1.008GlnHis: 1.008 ± 0.232
1.693GlnIle: 1.693 ± 0.275
1.129GlnLys: 1.129 ± 0.234
5.04GlnLeu: 5.04 ± 0.47
1.411GlnMet: 1.411 ± 0.204
0.927GlnAsn: 0.927 ± 0.207
3.266GlnPro: 3.266 ± 0.446
1.975GlnGln: 1.975 ± 0.27
3.709GlnArg: 3.709 ± 0.503
1.532GlnSer: 1.532 ± 0.22
1.734GlnThr: 1.734 ± 0.303
2.782GlnVal: 2.782 ± 0.312
0.685GlnTrp: 0.685 ± 0.168
1.008GlnTyr: 1.008 ± 0.178
0.0GlnXaa: 0.0 ± 0.0
Arg
7.257ArgAla: 7.257 ± 0.518
1.492ArgCys: 1.492 ± 0.339
4.274ArgAsp: 4.274 ± 0.424
3.911ArgGlu: 3.911 ± 0.448
1.774ArgPhe: 1.774 ± 0.307
5.886ArgGly: 5.886 ± 0.471
2.056ArgHis: 2.056 ± 0.35
2.298ArgIle: 2.298 ± 0.378
2.741ArgLys: 2.741 ± 0.424
6.451ArgLeu: 6.451 ± 0.55
1.975ArgMet: 1.975 ± 0.293
1.895ArgAsn: 1.895 ± 0.319
4.153ArgPro: 4.153 ± 0.504
2.58ArgGln: 2.58 ± 0.35
6.249ArgArg: 6.249 ± 0.736
4.032ArgSer: 4.032 ± 0.435
3.145ArgThr: 3.145 ± 0.34
5.483ArgVal: 5.483 ± 0.49
1.895ArgTrp: 1.895 ± 0.283
2.862ArgTyr: 2.862 ± 0.405
0.0ArgXaa: 0.0 ± 0.0
Ser
4.354SerAla: 4.354 ± 0.382
0.484SerCys: 0.484 ± 0.135
3.709SerAsp: 3.709 ± 0.438
2.58SerGlu: 2.58 ± 0.322
1.169SerPhe: 1.169 ± 0.217
5.362SerGly: 5.362 ± 0.676
1.25SerHis: 1.25 ± 0.224
1.492SerIle: 1.492 ± 0.315
1.29SerLys: 1.29 ± 0.246
2.983SerLeu: 2.983 ± 0.345
1.29SerMet: 1.29 ± 0.228
1.895SerAsn: 1.895 ± 0.245
2.782SerPro: 2.782 ± 0.381
2.379SerGln: 2.379 ± 0.275
4.032SerArg: 4.032 ± 0.462
2.5SerSer: 2.5 ± 0.333
2.661SerThr: 2.661 ± 0.331
4.032SerVal: 4.032 ± 0.392
1.089SerTrp: 1.089 ± 0.203
1.29SerTyr: 1.29 ± 0.235
0.0SerXaa: 0.0 ± 0.0
Thr
8.023ThrAla: 8.023 ± 0.645
0.806ThrCys: 0.806 ± 0.203
4.394ThrAsp: 4.394 ± 0.359
3.669ThrGlu: 3.669 ± 0.423
1.814ThrPhe: 1.814 ± 0.277
5.846ThrGly: 5.846 ± 0.578
1.33ThrHis: 1.33 ± 0.224
2.459ThrIle: 2.459 ± 0.318
1.975ThrLys: 1.975 ± 0.313
4.193ThrLeu: 4.193 ± 0.425
1.129ThrMet: 1.129 ± 0.197
2.258ThrAsn: 2.258 ± 0.313
5.201ThrPro: 5.201 ± 0.5
1.572ThrGln: 1.572 ± 0.301
3.951ThrArg: 3.951 ± 0.421
2.741ThrSer: 2.741 ± 0.36
5.322ThrThr: 5.322 ± 0.513
5.765ThrVal: 5.765 ± 0.513
1.29ThrTrp: 1.29 ± 0.24
1.371ThrTyr: 1.371 ± 0.262
0.0ThrXaa: 0.0 ± 0.0
Val
9.232ValAla: 9.232 ± 0.739
1.089ValCys: 1.089 ± 0.21
4.354ValAsp: 4.354 ± 0.354
5.08ValGlu: 5.08 ± 0.485
2.419ValPhe: 2.419 ± 0.312
6.491ValGly: 6.491 ± 0.585
2.016ValHis: 2.016 ± 0.326
3.104ValIle: 3.104 ± 0.381
2.298ValLys: 2.298 ± 0.347
5.685ValLeu: 5.685 ± 0.44
2.137ValMet: 2.137 ± 0.293
2.621ValAsn: 2.621 ± 0.272
4.919ValPro: 4.919 ± 0.47
2.379ValGln: 2.379 ± 0.375
3.749ValArg: 3.749 ± 0.398
3.024ValSer: 3.024 ± 0.35
5.564ValThr: 5.564 ± 0.472
7.055ValVal: 7.055 ± 0.66
1.693ValTrp: 1.693 ± 0.306
1.734ValTyr: 1.734 ± 0.298
0.0ValXaa: 0.0 ± 0.0
Trp
1.572TrpAla: 1.572 ± 0.22
0.363TrpCys: 0.363 ± 0.156
1.653TrpAsp: 1.653 ± 0.266
0.806TrpGlu: 0.806 ± 0.164
0.645TrpPhe: 0.645 ± 0.172
1.411TrpGly: 1.411 ± 0.246
0.927TrpHis: 0.927 ± 0.227
0.524TrpIle: 0.524 ± 0.127
0.484TrpLys: 0.484 ± 0.134
2.096TrpLeu: 2.096 ± 0.318
0.645TrpMet: 0.645 ± 0.201
0.645TrpAsn: 0.645 ± 0.176
0.968TrpPro: 0.968 ± 0.213
0.927TrpGln: 0.927 ± 0.184
1.895TrpArg: 1.895 ± 0.288
1.371TrpSer: 1.371 ± 0.204
1.451TrpThr: 1.451 ± 0.231
2.056TrpVal: 2.056 ± 0.32
0.484TrpTrp: 0.484 ± 0.139
0.282TrpTyr: 0.282 ± 0.081
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.459TyrAla: 2.459 ± 0.259
0.484TyrCys: 0.484 ± 0.123
1.492TyrAsp: 1.492 ± 0.237
1.25TyrGlu: 1.25 ± 0.203
0.323TyrPhe: 0.323 ± 0.12
2.58TyrGly: 2.58 ± 0.28
0.564TyrHis: 0.564 ± 0.166
0.806TyrIle: 0.806 ± 0.194
0.484TyrLys: 0.484 ± 0.136
2.016TyrLeu: 2.016 ± 0.257
0.323TyrMet: 0.323 ± 0.108
0.806TyrAsn: 0.806 ± 0.223
1.29TyrPro: 1.29 ± 0.232
1.169TyrGln: 1.169 ± 0.174
2.661TyrArg: 2.661 ± 0.407
1.492TyrSer: 1.492 ± 0.216
1.653TyrThr: 1.653 ± 0.284
1.814TyrVal: 1.814 ± 0.216
0.645TyrTrp: 0.645 ± 0.167
0.403TyrTyr: 0.403 ± 0.115
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 135 proteins (24805 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski