Chrysochromulina ericina virus (CeV01)
Average proteome isoelectric point is 7.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 512 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N9R0B7|A0A0N9R0B7_CEV01 Ubox/RING superfamily domain containing protein OS=Chrysochromulina ericina virus OX=455364 GN=ceV_196 PE=4 SV=1
MM1 pKa = 7.61 SSGFQLPGSLFVTQTNLSDD20 pKa = 3.9 VGDD23 pKa = 3.7 SMNRR27 pKa = 11.84 VIQSEE32 pKa = 4.2 IEE34 pKa = 4.07 EE35 pKa = 4.27 VARR38 pKa = 11.84 TSNILASTNFAFYY51 pKa = 10.28 IQADD55 pKa = 3.98 YY56 pKa = 11.24 NINNHH61 pKa = 5.53 ISYY64 pKa = 9.55 EE65 pKa = 4.1 PSSSADD71 pKa = 3.6 GLDD74 pKa = 3.37 DD75 pKa = 4.76 AYY77 pKa = 10.88 AMSAITVDD85 pKa = 3.45 SATATNAVIEE95 pKa = 4.38 LQTADD100 pKa = 4.09 GVPVQAGYY108 pKa = 10.43 IDD110 pKa = 3.71 AQNRR114 pKa = 11.84 DD115 pKa = 3.44 QEE117 pKa = 4.63 TLGNFPVTLTVSVSNTGEE135 pKa = 3.82 MSSTITITGEE145 pKa = 4.21 GVVSSISEE153 pKa = 4.18 NDD155 pKa = 3.04 SKK157 pKa = 11.69 AQVVYY162 pKa = 9.85 WSGIEE167 pKa = 3.79 STPIVDD173 pKa = 3.39 ATSVDD178 pKa = 3.7 VHH180 pKa = 7.36 SSTLTVADD188 pKa = 5.39 LLAQYY193 pKa = 10.25 QSQLQSIEE201 pKa = 4.02 SQYY204 pKa = 11.77 NNINMIEE211 pKa = 3.99 TWRR214 pKa = 11.84 IEE216 pKa = 3.79 ILGIDD221 pKa = 3.64 AGVSNPLSQHH231 pKa = 4.81 ARR233 pKa = 11.84 AVNKK237 pKa = 10.35 AGSQSVFSPGDD248 pKa = 3.39 KK249 pKa = 9.88 IVCQNTFSYY258 pKa = 10.65 EE259 pKa = 4.09 VVINDD264 pKa = 3.68 YY265 pKa = 10.83 QEE267 pKa = 4.14 IAPTTIVSSTNVYY280 pKa = 10.38 GVLQQII286 pKa = 4.22
Molecular weight: 30.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.834
IPC_protein 3.821
Toseland 3.617
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.656
Grimsley 3.528
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.668
EMBOSS 3.732
Sillero 3.935
Patrickios 0.604
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.84
Protein with the highest isoelectric point:
>tr|A0A0N9R4A1|A0A0N9R4A1_CEV01 Uncharacterized protein OS=Chrysochromulina ericina virus OX=455364 GN=ceV_445 PE=4 SV=1
MM1 pKa = 7.51 IGGARR6 pKa = 11.84 RR7 pKa = 11.84 TRR9 pKa = 11.84 RR10 pKa = 11.84 GGRR13 pKa = 11.84 SRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 GGMRR21 pKa = 11.84 GSQNLALNALLTGMVLSRR39 pKa = 11.84 GKK41 pKa = 9.93 KK42 pKa = 9.1 RR43 pKa = 11.84 KK44 pKa = 8.67 GKK46 pKa = 9.89 KK47 pKa = 8.36 SRR49 pKa = 11.84 RR50 pKa = 11.84 LSKK53 pKa = 10.31 LSRR56 pKa = 11.84 RR57 pKa = 11.84 GGRR60 pKa = 11.84 TRR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 3.36
Molecular weight: 7.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.497
IPC2_protein 11.491
IPC_protein 13.1
Toseland 13.261
ProMoST 13.759
Dawson 13.261
Bjellqvist 13.261
Wikipedia 13.744
Rodwell 12.881
Grimsley 13.305
Solomon 13.759
Lehninger 13.656
Nozaki 13.261
DTASelect 13.261
Thurlkill 13.261
EMBOSS 13.759
Sillero 13.261
Patrickios 12.603
IPC_peptide 13.759
IPC2_peptide 12.749
IPC2.peptide.svr19 9.37
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
512
0
512
143154
41
2317
279.6
32.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.523 ± 0.11
1.703 ± 0.051
5.694 ± 0.078
5.977 ± 0.138
4.396 ± 0.068
4.564 ± 0.119
1.702 ± 0.048
9.902 ± 0.162
9.496 ± 0.154
8.83 ± 0.107
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.022 ± 0.057
9.205 ± 0.16
3.641 ± 0.104
3.432 ± 0.094
3.456 ± 0.091
6.7 ± 0.091
5.475 ± 0.083
4.281 ± 0.082
0.958 ± 0.037
5.042 ± 0.09
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here