Amino acid dipepetide frequency for Streptomyces sp. T1317-0309

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.684AlaAla: 19.684 ± 0.236
1.287AlaCys: 1.287 ± 0.048
7.513AlaAsp: 7.513 ± 0.101
7.592AlaGlu: 7.592 ± 0.125
3.36AlaPhe: 3.36 ± 0.07
11.909AlaGly: 11.909 ± 0.143
2.939AlaHis: 2.939 ± 0.061
3.512AlaIle: 3.512 ± 0.074
2.666AlaLys: 2.666 ± 0.074
13.127AlaLeu: 13.127 ± 0.178
2.488AlaMet: 2.488 ± 0.056
1.858AlaAsn: 1.858 ± 0.048
6.914AlaPro: 6.914 ± 0.114
3.93AlaGln: 3.93 ± 0.068
10.494AlaArg: 10.494 ± 0.129
6.525AlaSer: 6.525 ± 0.097
7.071AlaThr: 7.071 ± 0.096
11.565AlaVal: 11.565 ± 0.131
1.857AlaTrp: 1.857 ± 0.045
2.44AlaTyr: 2.44 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
1.361CysAla: 1.361 ± 0.052
0.253CysCys: 0.253 ± 0.021
0.573CysAsp: 0.573 ± 0.032
0.407CysGlu: 0.407 ± 0.024
0.272CysPhe: 0.272 ± 0.02
1.299CysGly: 1.299 ± 0.05
0.27CysHis: 0.27 ± 0.017
0.232CysIle: 0.232 ± 0.019
0.11CysLys: 0.11 ± 0.013
0.828CysLeu: 0.828 ± 0.038
0.217CysMet: 0.217 ± 0.018
0.167CysAsn: 0.167 ± 0.015
0.857CysPro: 0.857 ± 0.034
0.244CysGln: 0.244 ± 0.018
1.097CysArg: 1.097 ± 0.043
0.865CysSer: 0.865 ± 0.041
0.77CysThr: 0.77 ± 0.033
0.722CysVal: 0.722 ± 0.029
0.267CysTrp: 0.267 ± 0.02
0.127CysTyr: 0.127 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.95AspAla: 6.95 ± 0.1
0.507AspCys: 0.507 ± 0.025
3.472AspAsp: 3.472 ± 0.075
3.529AspGlu: 3.529 ± 0.074
1.517AspPhe: 1.517 ± 0.046
6.056AspGly: 6.056 ± 0.099
1.548AspHis: 1.548 ± 0.041
1.917AspIle: 1.917 ± 0.063
1.206AspLys: 1.206 ± 0.044
5.81AspLeu: 5.81 ± 0.082
0.876AspMet: 0.876 ± 0.036
0.944AspAsn: 0.944 ± 0.036
4.245AspPro: 4.245 ± 0.074
1.522AspGln: 1.522 ± 0.04
5.003AspArg: 5.003 ± 0.086
2.681AspSer: 2.681 ± 0.058
3.171AspThr: 3.171 ± 0.068
4.431AspVal: 4.431 ± 0.077
0.975AspTrp: 0.975 ± 0.034
1.062AspTyr: 1.062 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
6.589GluAla: 6.589 ± 0.108
0.405GluCys: 0.405 ± 0.026
2.669GluAsp: 2.669 ± 0.059
3.294GluGlu: 3.294 ± 0.081
1.351GluPhe: 1.351 ± 0.049
3.988GluGly: 3.988 ± 0.075
1.526GluHis: 1.526 ± 0.046
2.137GluIle: 2.137 ± 0.058
1.331GluLys: 1.331 ± 0.047
6.027GluLeu: 6.027 ± 0.095
0.85GluMet: 0.85 ± 0.033
0.989GluAsn: 0.989 ± 0.033
3.145GluPro: 3.145 ± 0.07
2.178GluGln: 2.178 ± 0.06
5.224GluArg: 5.224 ± 0.097
2.553GluSer: 2.553 ± 0.065
2.653GluThr: 2.653 ± 0.06
4.08GluVal: 4.08 ± 0.086
0.79GluTrp: 0.79 ± 0.034
1.042GluTyr: 1.042 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
3.322PheAla: 3.322 ± 0.073
0.279PheCys: 0.279 ± 0.018
1.763PheAsp: 1.763 ± 0.047
1.355PheGlu: 1.355 ± 0.044
0.834PhePhe: 0.834 ± 0.035
2.677PheGly: 2.677 ± 0.063
0.65PheHis: 0.65 ± 0.029
0.766PheIle: 0.766 ± 0.034
0.493PheLys: 0.493 ± 0.026
2.382PheLeu: 2.382 ± 0.061
0.39PheMet: 0.39 ± 0.023
0.542PheAsn: 0.542 ± 0.028
1.338PhePro: 1.338 ± 0.037
0.688PheGln: 0.688 ± 0.032
1.902PheArg: 1.902 ± 0.049
1.394PheSer: 1.394 ± 0.042
1.999PheThr: 1.999 ± 0.047
2.108PheVal: 2.108 ± 0.053
0.417PheTrp: 0.417 ± 0.023
0.541PheTyr: 0.541 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
10.041GlyAla: 10.041 ± 0.129
1.119GlyCys: 1.119 ± 0.039
4.972GlyAsp: 4.972 ± 0.078
4.488GlyGlu: 4.488 ± 0.087
2.75GlyPhe: 2.75 ± 0.062
8.273GlyGly: 8.273 ± 0.139
2.542GlyHis: 2.542 ± 0.064
3.2GlyIle: 3.2 ± 0.067
2.266GlyLys: 2.266 ± 0.059
8.48GlyLeu: 8.48 ± 0.113
1.916GlyMet: 1.916 ± 0.047
1.8GlyAsn: 1.8 ± 0.054
5.546GlyPro: 5.546 ± 0.09
2.716GlyGln: 2.716 ± 0.057
8.996GlyArg: 8.996 ± 0.136
5.679GlySer: 5.679 ± 0.095
6.372GlyThr: 6.372 ± 0.099
6.813GlyVal: 6.813 ± 0.091
1.761GlyTrp: 1.761 ± 0.046
2.055GlyTyr: 2.055 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
2.851HisAla: 2.851 ± 0.059
0.271HisCys: 0.271 ± 0.019
1.516HisAsp: 1.516 ± 0.038
1.228HisGlu: 1.228 ± 0.039
0.699HisPhe: 0.699 ± 0.031
2.724HisGly: 2.724 ± 0.055
0.936HisHis: 0.936 ± 0.038
0.775HisIle: 0.775 ± 0.032
0.389HisLys: 0.389 ± 0.022
2.452HisLeu: 2.452 ± 0.053
0.44HisMet: 0.44 ± 0.025
0.44HisAsn: 0.44 ± 0.025
2.016HisPro: 2.016 ± 0.055
0.751HisGln: 0.751 ± 0.03
2.852HisArg: 2.852 ± 0.068
1.19HisSer: 1.19 ± 0.043
1.403HisThr: 1.403 ± 0.042
1.753HisVal: 1.753 ± 0.045
0.421HisTrp: 0.421 ± 0.022
0.511HisTyr: 0.511 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
4.649IleAla: 4.649 ± 0.088
0.373IleCys: 0.373 ± 0.022
2.194IleAsp: 2.194 ± 0.065
1.986IleGlu: 1.986 ± 0.051
0.664IlePhe: 0.664 ± 0.032
3.348IleGly: 3.348 ± 0.086
0.729IleHis: 0.729 ± 0.03
0.866IleIle: 0.866 ± 0.029
0.743IleLys: 0.743 ± 0.032
2.273IleLeu: 2.273 ± 0.06
0.423IleMet: 0.423 ± 0.022
0.698IleAsn: 0.698 ± 0.039
1.789IlePro: 1.789 ± 0.053
0.762IleGln: 0.762 ± 0.032
2.427IleArg: 2.427 ± 0.057
1.787IleSer: 1.787 ± 0.052
2.344IleThr: 2.344 ± 0.058
2.749IleVal: 2.749 ± 0.07
0.366IleTrp: 0.366 ± 0.021
0.546IleTyr: 0.546 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
2.892LysAla: 2.892 ± 0.079
0.127LysCys: 0.127 ± 0.014
1.341LysAsp: 1.341 ± 0.048
1.175LysGlu: 1.175 ± 0.049
0.454LysPhe: 0.454 ± 0.027
1.818LysGly: 1.818 ± 0.058
0.42LysHis: 0.42 ± 0.021
0.895LysIle: 0.895 ± 0.037
0.867LysLys: 0.867 ± 0.045
1.875LysLeu: 1.875 ± 0.057
0.414LysMet: 0.414 ± 0.024
0.589LysAsn: 0.589 ± 0.034
1.404LysPro: 1.404 ± 0.049
0.725LysGln: 0.725 ± 0.034
1.587LysArg: 1.587 ± 0.055
1.216LysSer: 1.216 ± 0.046
1.335LysThr: 1.335 ± 0.05
1.78LysVal: 1.78 ± 0.055
0.267LysTrp: 0.267 ± 0.019
0.464LysTyr: 0.464 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
13.139LeuAla: 13.139 ± 0.162
0.95LeuCys: 0.95 ± 0.036
5.99LeuAsp: 5.99 ± 0.097
4.59LeuGlu: 4.59 ± 0.083
2.341LeuPhe: 2.341 ± 0.056
8.135LeuGly: 8.135 ± 0.119
2.398LeuHis: 2.398 ± 0.059
3.193LeuIle: 3.193 ± 0.073
1.919LeuLys: 1.919 ± 0.065
10.173LeuLeu: 10.173 ± 0.149
1.574LeuMet: 1.574 ± 0.046
1.713LeuAsn: 1.713 ± 0.041
6.151LeuPro: 6.151 ± 0.111
2.382LeuGln: 2.382 ± 0.06
8.408LeuArg: 8.408 ± 0.103
5.212LeuSer: 5.212 ± 0.089
6.525LeuThr: 6.525 ± 0.095
8.122LeuVal: 8.122 ± 0.107
1.315LeuTrp: 1.315 ± 0.043
1.759LeuTyr: 1.759 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
2.332MetAla: 2.332 ± 0.055
0.182MetCys: 0.182 ± 0.014
0.953MetAsp: 0.953 ± 0.039
0.805MetGlu: 0.805 ± 0.034
0.442MetPhe: 0.442 ± 0.022
1.239MetGly: 1.239 ± 0.04
0.416MetHis: 0.416 ± 0.024
0.657MetIle: 0.657 ± 0.031
0.433MetLys: 0.433 ± 0.025
1.696MetLeu: 1.696 ± 0.046
0.288MetMet: 0.288 ± 0.022
0.437MetAsn: 0.437 ± 0.025
1.201MetPro: 1.201 ± 0.041
0.494MetGln: 0.494 ± 0.028
1.574MetArg: 1.574 ± 0.043
1.561MetSer: 1.561 ± 0.047
1.68MetThr: 1.68 ± 0.042
1.256MetVal: 1.256 ± 0.037
0.214MetTrp: 0.214 ± 0.017
0.367MetTyr: 0.367 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
2.247AsnAla: 2.247 ± 0.056
0.25AsnCys: 0.25 ± 0.02
1.015AsnAsp: 1.015 ± 0.037
0.867AsnGlu: 0.867 ± 0.035
0.455AsnPhe: 0.455 ± 0.026
1.997AsnGly: 1.997 ± 0.061
0.415AsnHis: 0.415 ± 0.025
0.644AsnIle: 0.644 ± 0.029
0.452AsnLys: 0.452 ± 0.025
1.618AsnLeu: 1.618 ± 0.05
0.323AsnMet: 0.323 ± 0.018
0.498AsnAsn: 0.498 ± 0.025
1.343AsnPro: 1.343 ± 0.044
0.538AsnGln: 0.538 ± 0.028
1.369AsnArg: 1.369 ± 0.042
1.036AsnSer: 1.036 ± 0.041
1.124AsnThr: 1.124 ± 0.035
1.363AsnVal: 1.363 ± 0.042
0.331AsnTrp: 0.331 ± 0.02
0.372AsnTyr: 0.372 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
8.482ProAla: 8.482 ± 0.12
0.726ProCys: 0.726 ± 0.03
4.312ProAsp: 4.312 ± 0.082
3.9ProGlu: 3.9 ± 0.075
1.453ProPhe: 1.453 ± 0.045
7.116ProGly: 7.116 ± 0.116
1.635ProHis: 1.635 ± 0.051
1.307ProIle: 1.307 ± 0.044
1.255ProLys: 1.255 ± 0.05
5.01ProLeu: 5.01 ± 0.074
1.137ProMet: 1.137 ± 0.037
1.023ProAsn: 1.023 ± 0.038
4.443ProPro: 4.443 ± 0.102
1.82ProGln: 1.82 ± 0.051
5.143ProArg: 5.143 ± 0.091
4.131ProSer: 4.131 ± 0.091
3.773ProThr: 3.773 ± 0.084
5.218ProVal: 5.218 ± 0.086
1.036ProTrp: 1.036 ± 0.036
1.378ProTyr: 1.378 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
3.74GlnAla: 3.74 ± 0.073
0.259GlnCys: 0.259 ± 0.02
1.477GlnAsp: 1.477 ± 0.042
1.497GlnGlu: 1.497 ± 0.051
0.665GlnPhe: 0.665 ± 0.029
2.417GlnGly: 2.417 ± 0.067
0.813GlnHis: 0.813 ± 0.035
1.118GlnIle: 1.118 ± 0.042
0.675GlnLys: 0.675 ± 0.033
3.01GlnLeu: 3.01 ± 0.053
0.557GlnMet: 0.557 ± 0.028
0.56GlnAsn: 0.56 ± 0.031
1.943GlnPro: 1.943 ± 0.058
1.403GlnGln: 1.403 ± 0.063
2.73GlnArg: 2.73 ± 0.067
1.407GlnSer: 1.407 ± 0.045
1.477GlnThr: 1.477 ± 0.044
2.334GlnVal: 2.334 ± 0.06
0.473GlnTrp: 0.473 ± 0.023
0.629GlnTyr: 0.629 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
10.08ArgAla: 10.08 ± 0.131
1.182ArgCys: 1.182 ± 0.048
4.185ArgAsp: 4.185 ± 0.081
4.392ArgGlu: 4.392 ± 0.092
2.226ArgPhe: 2.226 ± 0.051
6.475ArgGly: 6.475 ± 0.107
2.851ArgHis: 2.851 ± 0.073
3.292ArgIle: 3.292 ± 0.074
1.728ArgLys: 1.728 ± 0.058
8.723ArgLeu: 8.723 ± 0.125
2.085ArgMet: 2.085 ± 0.05
1.597ArgAsn: 1.597 ± 0.048
6.52ArgPro: 6.52 ± 0.122
2.806ArgGln: 2.806 ± 0.069
10.64ArgArg: 10.64 ± 0.175
5.616ArgSer: 5.616 ± 0.091
6.222ArgThr: 6.222 ± 0.086
5.583ArgVal: 5.583 ± 0.094
1.599ArgTrp: 1.599 ± 0.043
1.783ArgTyr: 1.783 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
7.412SerAla: 7.412 ± 0.118
0.79SerCys: 0.79 ± 0.032
2.879SerAsp: 2.879 ± 0.068
2.524SerGlu: 2.524 ± 0.062
1.461SerPhe: 1.461 ± 0.045
6.28SerGly: 6.28 ± 0.099
1.268SerHis: 1.268 ± 0.04
1.549SerIle: 1.549 ± 0.04
1.15SerLys: 1.15 ± 0.046
4.711SerLeu: 4.711 ± 0.083
1.207SerMet: 1.207 ± 0.041
0.936SerAsn: 0.936 ± 0.035
4.228SerPro: 4.228 ± 0.089
1.398SerGln: 1.398 ± 0.045
4.931SerArg: 4.931 ± 0.09
4.057SerSer: 4.057 ± 0.109
3.959SerThr: 3.959 ± 0.096
4.297SerVal: 4.297 ± 0.08
1.147SerTrp: 1.147 ± 0.04
1.204SerTyr: 1.204 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
9.048ThrAla: 9.048 ± 0.115
0.686ThrCys: 0.686 ± 0.038
3.683ThrAsp: 3.683 ± 0.072
3.039ThrGlu: 3.039 ± 0.068
1.636ThrPhe: 1.636 ± 0.047
6.835ThrGly: 6.835 ± 0.102
1.385ThrHis: 1.385 ± 0.043
1.783ThrIle: 1.783 ± 0.056
1.328ThrLys: 1.328 ± 0.048
5.494ThrLeu: 5.494 ± 0.089
1.038ThrMet: 1.038 ± 0.033
1.066ThrAsn: 1.066 ± 0.039
4.39ThrPro: 4.39 ± 0.082
1.422ThrGln: 1.422 ± 0.038
4.621ThrArg: 4.621 ± 0.09
3.981ThrSer: 3.981 ± 0.091
4.317ThrThr: 4.317 ± 0.105
6.013ThrVal: 6.013 ± 0.098
1.005ThrTrp: 1.005 ± 0.039
1.411ThrTyr: 1.411 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
9.765ValAla: 9.765 ± 0.136
0.865ValCys: 0.865 ± 0.036
4.688ValAsp: 4.688 ± 0.084
4.31ValGlu: 4.31 ± 0.084
2.293ValPhe: 2.293 ± 0.055
6.115ValGly: 6.115 ± 0.09
2.046ValHis: 2.046 ± 0.056
2.767ValIle: 2.767 ± 0.064
1.766ValLys: 1.766 ± 0.056
8.728ValLeu: 8.728 ± 0.137
1.392ValMet: 1.392 ± 0.041
1.499ValAsn: 1.499 ± 0.042
4.806ValPro: 4.806 ± 0.075
2.179ValGln: 2.179 ± 0.053
7.119ValArg: 7.119 ± 0.115
4.328ValSer: 4.328 ± 0.071
5.504ValThr: 5.504 ± 0.093
7.404ValVal: 7.404 ± 0.122
1.114ValTrp: 1.114 ± 0.04
1.539ValTyr: 1.539 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
1.697TrpAla: 1.697 ± 0.05
0.215TrpCys: 0.215 ± 0.018
0.773TrpAsp: 0.773 ± 0.029
0.695TrpGlu: 0.695 ± 0.033
0.442TrpPhe: 0.442 ± 0.023
1.029TrpGly: 1.029 ± 0.04
0.443TrpHis: 0.443 ± 0.024
0.569TrpIle: 0.569 ± 0.024
0.436TrpLys: 0.436 ± 0.024
1.706TrpLeu: 1.706 ± 0.048
0.355TrpMet: 0.355 ± 0.024
0.44TrpAsn: 0.44 ± 0.022
1.046TrpPro: 1.046 ± 0.039
0.635TrpGln: 0.635 ± 0.03
1.591TrpArg: 1.591 ± 0.048
1.146TrpSer: 1.146 ± 0.037
1.234TrpThr: 1.234 ± 0.042
0.967TrpVal: 0.967 ± 0.038
0.388TrpTrp: 0.388 ± 0.02
0.323TrpTyr: 0.323 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.45TyrAla: 2.45 ± 0.059
0.213TyrCys: 0.213 ± 0.015
1.455TyrAsp: 1.455 ± 0.049
1.147TyrGlu: 1.147 ± 0.041
0.522TyrPhe: 0.522 ± 0.027
2.055TyrGly: 2.055 ± 0.052
0.386TyrHis: 0.386 ± 0.023
0.534TyrIle: 0.534 ± 0.027
0.43TyrLys: 0.43 ± 0.026
1.866TyrLeu: 1.866 ± 0.05
0.255TyrMet: 0.255 ± 0.02
0.464TyrAsn: 0.464 ± 0.027
0.972TyrPro: 0.972 ± 0.035
0.592TyrGln: 0.592 ± 0.03
1.877TyrArg: 1.877 ± 0.05
1.026TyrSer: 1.026 ± 0.04
1.247TyrThr: 1.247 ± 0.046
1.669TyrVal: 1.669 ± 0.048
0.39TyrTrp: 0.39 ± 0.023
0.471TyrTyr: 0.471 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3759 proteins (771320 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski