Vanrija humicola

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Trichosporonales; Trichosporonaceae; Vanrija

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4373 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A7D8YYK0|A0A7D8YYK0_9TREE Barwin domain-containing protein OS=Vanrija humicola OX=5417 GN=VHUM_03241 PE=4 SV=1
MM1 pKa = 7.14VSYY4 pKa = 10.97YY5 pKa = 10.84DD6 pKa = 3.55EE7 pKa = 5.96LEE9 pKa = 4.94IEE11 pKa = 4.3DD12 pKa = 4.53FAWDD16 pKa = 3.7DD17 pKa = 3.53VAKK20 pKa = 10.26VYY22 pKa = 10.19HH23 pKa = 5.83YY24 pKa = 8.17PCPCGDD30 pKa = 3.22RR31 pKa = 11.84FEE33 pKa = 4.36ISKK36 pKa = 10.54AQLRR40 pKa = 11.84DD41 pKa = 3.26GEE43 pKa = 4.69EE44 pKa = 3.8IATCPSCSLIVRR56 pKa = 11.84VVYY59 pKa = 10.54DD60 pKa = 3.44YY61 pKa = 11.1MDD63 pKa = 3.96YY64 pKa = 11.35EE65 pKa = 4.78DD66 pKa = 6.04YY67 pKa = 8.97EE68 pKa = 4.51TTDD71 pKa = 3.59DD72 pKa = 4.13EE73 pKa = 4.55EE74 pKa = 6.34GEE76 pKa = 4.16EE77 pKa = 4.86SEE79 pKa = 6.43DD80 pKa = 3.78EE81 pKa = 4.39AQTTPTTSTSPSPSPNAQTTGTGAEE106 pKa = 3.93EE107 pKa = 4.2RR108 pKa = 11.84GRR110 pKa = 11.84ADD112 pKa = 4.35GKK114 pKa = 10.82ADD116 pKa = 4.2CPDD119 pKa = 4.22DD120 pKa = 4.11PASLSKK126 pKa = 11.0DD127 pKa = 3.41LGNLALGKK135 pKa = 10.25GRR137 pKa = 11.84SADD140 pKa = 3.62EE141 pKa = 4.04PP142 pKa = 4.03

Molecular weight:
15.57 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A7D8VBF8|A0A7D8VBF8_9TREE Isochorismatase domain-containing protein OS=Vanrija humicola OX=5417 GN=VHUM_00174 PE=3 SV=1
MM1 pKa = 6.73STEE4 pKa = 4.29GKK6 pKa = 9.67VSSVGVTSIGMAACTLGTVGRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84HH30 pKa = 4.81TQHH33 pKa = 6.6TLTLFLRR40 pKa = 11.84PSPARR45 pKa = 11.84LPSLGRR51 pKa = 11.84LASPCLLRR59 pKa = 11.84LSRR62 pKa = 11.84SRR64 pKa = 11.84PPRR67 pKa = 11.84RR68 pKa = 11.84ARR70 pKa = 11.84SASRR74 pKa = 11.84SSTLVSATLTTTPSPARR91 pKa = 11.84TPRR94 pKa = 11.84VPSPSSSATRR104 pKa = 11.84AVVSSSRR111 pKa = 11.84SAPALPTSRR120 pKa = 11.84SATTSFPSTPPSARR134 pKa = 11.84SASSAFRR141 pKa = 11.84ARR143 pKa = 11.84PTCVVPSVLPRR154 pKa = 11.84ARR156 pKa = 11.84ASCPTAPRR164 pKa = 11.84ASPARR169 pKa = 11.84ASLSTTSWAPRR180 pKa = 11.84PSRR183 pKa = 11.84STRR186 pKa = 11.84SCPSTRR192 pKa = 11.84SSLSTPRR199 pKa = 11.84RR200 pKa = 11.84RR201 pKa = 11.84SRR203 pKa = 11.84RR204 pKa = 11.84PVSSAAVSPPATVLPPRR221 pKa = 11.84RR222 pKa = 11.84LALRR226 pKa = 11.84SRR228 pKa = 11.84TLPSSVSAVSASRR241 pKa = 11.84LSRR244 pKa = 11.84VPVPRR249 pKa = 11.84RR250 pKa = 11.84PLASLPSTPTPRR262 pKa = 11.84RR263 pKa = 11.84RR264 pKa = 11.84SGLRR268 pKa = 11.84SLVLPTLSTPSTSPRR283 pKa = 11.84ARR285 pKa = 11.84ASSTTT290 pKa = 3.27

Molecular weight:
30.37 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4373

0

4373

2036452

9

4519

465.7

50.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.879 ± 0.05

1.002 ± 0.011

5.643 ± 0.029

5.868 ± 0.037

3.606 ± 0.023

7.613 ± 0.036

2.265 ± 0.017

4.482 ± 0.025

4.547 ± 0.034

9.219 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.912 ± 0.014

3.047 ± 0.018

6.358 ± 0.045

3.375 ± 0.027

6.232 ± 0.028

7.158 ± 0.039

5.756 ± 0.021

6.949 ± 0.028

1.497 ± 0.014

2.592 ± 0.019

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski