Vanrija humicola
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4373 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D8YYK0|A0A7D8YYK0_9TREE Barwin domain-containing protein OS=Vanrija humicola OX=5417 GN=VHUM_03241 PE=4 SV=1
MM1 pKa = 7.14 VSYY4 pKa = 10.97 YY5 pKa = 10.84 DD6 pKa = 3.55 EE7 pKa = 5.96 LEE9 pKa = 4.94 IEE11 pKa = 4.3 DD12 pKa = 4.53 FAWDD16 pKa = 3.7 DD17 pKa = 3.53 VAKK20 pKa = 10.26 VYY22 pKa = 10.19 HH23 pKa = 5.83 YY24 pKa = 8.17 PCPCGDD30 pKa = 3.22 RR31 pKa = 11.84 FEE33 pKa = 4.36 ISKK36 pKa = 10.54 AQLRR40 pKa = 11.84 DD41 pKa = 3.26 GEE43 pKa = 4.69 EE44 pKa = 3.8 IATCPSCSLIVRR56 pKa = 11.84 VVYY59 pKa = 10.54 DD60 pKa = 3.44 YY61 pKa = 11.1 MDD63 pKa = 3.96 YY64 pKa = 11.35 EE65 pKa = 4.78 DD66 pKa = 6.04 YY67 pKa = 8.97 EE68 pKa = 4.51 TTDD71 pKa = 3.59 DD72 pKa = 4.13 EE73 pKa = 4.55 EE74 pKa = 6.34 GEE76 pKa = 4.16 EE77 pKa = 4.86 SEE79 pKa = 6.43 DD80 pKa = 3.78 EE81 pKa = 4.39 AQTTPTTSTSPSPSPNAQTTGTGAEE106 pKa = 3.93 EE107 pKa = 4.2 RR108 pKa = 11.84 GRR110 pKa = 11.84 ADD112 pKa = 4.35 GKK114 pKa = 10.82 ADD116 pKa = 4.2 CPDD119 pKa = 4.22 DD120 pKa = 4.11 PASLSKK126 pKa = 11.0 DD127 pKa = 3.41 LGNLALGKK135 pKa = 10.25 GRR137 pKa = 11.84 SADD140 pKa = 3.62 EE141 pKa = 4.04 PP142 pKa = 4.03
Molecular weight: 15.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.63
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.656
Grimsley 3.528
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.668
EMBOSS 3.732
Sillero 3.948
Patrickios 1.1
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A7D8VBF8|A0A7D8VBF8_9TREE Isochorismatase domain-containing protein OS=Vanrija humicola OX=5417 GN=VHUM_00174 PE=3 SV=1
MM1 pKa = 6.73 STEE4 pKa = 4.29 GKK6 pKa = 9.67 VSSVGVTSIGMAACTLGTVGRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 HH30 pKa = 4.81 TQHH33 pKa = 6.6 TLTLFLRR40 pKa = 11.84 PSPARR45 pKa = 11.84 LPSLGRR51 pKa = 11.84 LASPCLLRR59 pKa = 11.84 LSRR62 pKa = 11.84 SRR64 pKa = 11.84 PPRR67 pKa = 11.84 RR68 pKa = 11.84 ARR70 pKa = 11.84 SASRR74 pKa = 11.84 SSTLVSATLTTTPSPARR91 pKa = 11.84 TPRR94 pKa = 11.84 VPSPSSSATRR104 pKa = 11.84 AVVSSSRR111 pKa = 11.84 SAPALPTSRR120 pKa = 11.84 SATTSFPSTPPSARR134 pKa = 11.84 SASSAFRR141 pKa = 11.84 ARR143 pKa = 11.84 PTCVVPSVLPRR154 pKa = 11.84 ARR156 pKa = 11.84 ASCPTAPRR164 pKa = 11.84 ASPARR169 pKa = 11.84 ASLSTTSWAPRR180 pKa = 11.84 PSRR183 pKa = 11.84 STRR186 pKa = 11.84 SCPSTRR192 pKa = 11.84 SSLSTPRR199 pKa = 11.84 RR200 pKa = 11.84 RR201 pKa = 11.84 SRR203 pKa = 11.84 RR204 pKa = 11.84 PVSSAAVSPPATVLPPRR221 pKa = 11.84 RR222 pKa = 11.84 LALRR226 pKa = 11.84 SRR228 pKa = 11.84 TLPSSVSAVSASRR241 pKa = 11.84 LSRR244 pKa = 11.84 VPVPRR249 pKa = 11.84 RR250 pKa = 11.84 PLASLPSTPTPRR262 pKa = 11.84 RR263 pKa = 11.84 RR264 pKa = 11.84 SGLRR268 pKa = 11.84 SLVLPTLSTPSTSPRR283 pKa = 11.84 ARR285 pKa = 11.84 ASSTTT290 pKa = 3.27
Molecular weight: 30.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.455
IPC2_protein 10.994
IPC_protein 12.603
Toseland 12.764
ProMoST 13.261
Dawson 12.764
Bjellqvist 12.764
Wikipedia 13.247
Rodwell 12.281
Grimsley 12.808
Solomon 13.261
Lehninger 13.173
Nozaki 12.764
DTASelect 12.764
Thurlkill 12.764
EMBOSS 13.261
Sillero 12.764
Patrickios 11.989
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4373
0
4373
2036452
9
4519
465.7
50.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.879 ± 0.05
1.002 ± 0.011
5.643 ± 0.029
5.868 ± 0.037
3.606 ± 0.023
7.613 ± 0.036
2.265 ± 0.017
4.482 ± 0.025
4.547 ± 0.034
9.219 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.912 ± 0.014
3.047 ± 0.018
6.358 ± 0.045
3.375 ± 0.027
6.232 ± 0.028
7.158 ± 0.039
5.756 ± 0.021
6.949 ± 0.028
1.497 ± 0.014
2.592 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here