Marinomonas phage P12026
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6S2Z9|I6S2Z9_9CAUD Uncharacterized protein OS=Marinomonas phage P12026 OX=1176423 GN=P12026_51 PE=4 SV=1
MM1 pKa = 7.54 INIATLRR8 pKa = 11.84 KK9 pKa = 9.33 LVNLRR14 pKa = 11.84 RR15 pKa = 11.84 KK16 pKa = 9.84 ADD18 pKa = 3.65 RR19 pKa = 11.84 FTDD22 pKa = 3.85 SLPMSINAAFFDD34 pKa = 3.84 NDD36 pKa = 3.75 LATSYY41 pKa = 11.39 YY42 pKa = 11.45 AMVDD46 pKa = 3.46 LLIDD50 pKa = 4.06 EE51 pKa = 5.21 LCVGCQADD59 pKa = 3.9 YY60 pKa = 11.46 VDD62 pKa = 3.53 WVLNEE67 pKa = 3.57 WHH69 pKa = 6.76 PTDD72 pKa = 4.62 NIMIVDD78 pKa = 3.99 GVEE81 pKa = 4.47 FNFKK85 pKa = 9.96 TEE87 pKa = 3.99 EE88 pKa = 3.71 EE89 pKa = 4.85 LYY91 pKa = 10.54 AHH93 pKa = 6.57 LVEE96 pKa = 4.37 YY97 pKa = 10.33 EE98 pKa = 4.12 GWSS101 pKa = 3.34
Molecular weight: 11.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.079
IPC2_protein 4.19
IPC_protein 4.113
Toseland 3.923
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 3.999
Rodwell 3.948
Grimsley 3.834
Solomon 4.075
Lehninger 4.037
Nozaki 4.202
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.012
Sillero 4.228
Patrickios 2.943
IPC_peptide 4.075
IPC2_peptide 4.215
IPC2.peptide.svr19 4.138
Protein with the highest isoelectric point:
>tr|I6R1J7|I6R1J7_9CAUD Phage exonuclease OS=Marinomonas phage P12026 OX=1176423 GN=P12026_50 PE=4 SV=1
MM1 pKa = 6.86 THH3 pKa = 6.41 NKK5 pKa = 9.99 FEE7 pKa = 4.54 DD8 pKa = 3.2 RR9 pKa = 11.84 RR10 pKa = 11.84 VNYY13 pKa = 10.29 GSATVPWSDD22 pKa = 4.75 KK23 pKa = 10.23 NNAWRR28 pKa = 11.84 LPCGKK33 pKa = 8.6 FTKK36 pKa = 10.27 DD37 pKa = 2.85 RR38 pKa = 11.84 NEE40 pKa = 4.23 ALNTAKK46 pKa = 10.6 RR47 pKa = 11.84 INRR50 pKa = 11.84 FFTGRR55 pKa = 11.84 VKK57 pKa = 11.16 NEE59 pKa = 3.72 GAVV62 pKa = 3.16
Molecular weight: 7.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.257
IPC2_protein 9.516
IPC_protein 9.78
Toseland 10.672
ProMoST 10.204
Dawson 10.745
Bjellqvist 10.365
Wikipedia 10.877
Rodwell 11.155
Grimsley 10.774
Solomon 10.818
Lehninger 10.818
Nozaki 10.657
DTASelect 10.35
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.672
Patrickios 10.95
IPC_peptide 10.833
IPC2_peptide 9.063
IPC2.peptide.svr19 8.64
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
9918
44
639
183.7
20.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.962 ± 0.735
1.018 ± 0.147
6.12 ± 0.332
6.927 ± 0.373
3.105 ± 0.19
6.765 ± 0.341
1.754 ± 0.208
6.211 ± 0.24
6.655 ± 0.482
7.743 ± 0.269
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.833 ± 0.189
4.477 ± 0.319
3.559 ± 0.261
3.862 ± 0.327
4.991 ± 0.298
7.209 ± 0.4
5.888 ± 0.362
6.322 ± 0.241
1.603 ± 0.151
2.995 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here