Amino acid dipepetide frequency for Glycine max (Soybean) (Glycine hispida)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.865AlaAla: 5.865 ± 0.022	1.24AlaCys: 1.24 ± 0.007	2.939AlaAsp: 2.939 ± 0.01	4.033AlaGlu: 4.033 ± 0.014	2.71AlaPhe: 2.71 ± 0.009	3.688AlaGly: 3.688 ± 0.013	1.312AlaHis: 1.312 ± 0.006	3.709AlaIle: 3.709 ± 0.011	3.829AlaLys: 3.829 ± 0.013	6.457AlaLeu: 6.457 ± 0.016	1.706AlaMet: 1.706 ± 0.008	2.654AlaAsn: 2.654 ± 0.009	2.762AlaPro: 2.762 ± 0.012	2.122AlaGln: 2.122 ± 0.009	3.211AlaArg: 3.211 ± 0.011	5.968AlaSer: 5.968 ± 0.017	3.617AlaThr: 3.617 ± 0.012	4.546AlaVal: 4.546 ± 0.014	0.739AlaTrp: 0.739 ± 0.005	1.761AlaTyr: 1.761 ± 0.008	0.0AlaXaa: 0.0 ± 0.0
Cys	0.999CysAla: 0.999 ± 0.006	0.575CysCys: 0.575 ± 0.005	0.911CysAsp: 0.911 ± 0.006	0.916CysGlu: 0.916 ± 0.006	0.958CysPhe: 0.958 ± 0.006	1.372CysGly: 1.372 ± 0.008	0.536CysHis: 0.536 ± 0.004	1.041CysIle: 1.041 ± 0.006	1.16CysLys: 1.16 ± 0.007	1.91CysLeu: 1.91 ± 0.008	0.477CysMet: 0.477 ± 0.004	0.923CysAsn: 0.923 ± 0.006	0.913CysPro: 0.913 ± 0.007	0.652CysGln: 0.652 ± 0.005	1.003CysArg: 1.003 ± 0.006	1.856CysSer: 1.856 ± 0.009	0.892CysThr: 0.892 ± 0.006	1.132CysVal: 1.132 ± 0.007	0.266CysTrp: 0.266 ± 0.003	0.579CysTyr: 0.579 ± 0.005	0.0CysXaa: 0.0 ± 0.0
Asp	3.441AspAla: 3.441 ± 0.012	0.949AspCys: 0.949 ± 0.007	3.655AspAsp: 3.655 ± 0.015	3.829AspGlu: 3.829 ± 0.013	2.333AspPhe: 2.333 ± 0.009	3.645AspGly: 3.645 ± 0.013	1.27AspHis: 1.27 ± 0.007	3.115AspIle: 3.115 ± 0.011	2.855AspLys: 2.855 ± 0.01	5.067AspLeu: 5.067 ± 0.015	1.302AspMet: 1.302 ± 0.008	2.295AspAsn: 2.295 ± 0.009	2.563AspPro: 2.563 ± 0.01	1.807AspGln: 1.807 ± 0.009	2.255AspArg: 2.255 ± 0.012	4.285AspSer: 4.285 ± 0.014	2.306AspThr: 2.306 ± 0.01	3.581AspVal: 3.581 ± 0.012	0.693AspTrp: 0.693 ± 0.005	1.541AspTyr: 1.541 ± 0.008	0.0AspXaa: 0.0 ± 0.0
Glu	4.601GluAla: 4.601 ± 0.015	0.955GluCys: 0.955 ± 0.006	3.923GluAsp: 3.923 ± 0.013	6.028GluGlu: 6.028 ± 0.025	2.353GluPhe: 2.353 ± 0.009	3.644GluGly: 3.644 ± 0.012	1.33GluHis: 1.33 ± 0.006	3.741GluIle: 3.741 ± 0.011	4.764GluLys: 4.764 ± 0.019	5.873GluLeu: 5.873 ± 0.018	1.659GluMet: 1.659 ± 0.008	3.289GluAsn: 3.289 ± 0.012	2.076GluPro: 2.076 ± 0.01	2.203GluGln: 2.203 ± 0.01	3.285GluArg: 3.285 ± 0.012	4.594GluSer: 4.594 ± 0.015	3.036GluThr: 3.036 ± 0.012	4.082GluVal: 4.082 ± 0.013	0.732GluTrp: 0.732 ± 0.005	1.706GluTyr: 1.706 ± 0.008	0.0GluXaa: 0.0 ± 0.0
Phe	2.446PheAla: 2.446 ± 0.01	0.958PheCys: 0.958 ± 0.005	2.322PheAsp: 2.322 ± 0.009	2.299PheGlu: 2.299 ± 0.009	2.009PhePhe: 2.009 ± 0.01	3.039PheGly: 3.039 ± 0.013	1.194PheHis: 1.194 ± 0.007	2.113PheIle: 2.113 ± 0.01	2.18PheLys: 2.18 ± 0.009	4.377PheLeu: 4.377 ± 0.015	0.976PheMet: 0.976 ± 0.006	1.825PheAsn: 1.825 ± 0.008	2.193PhePro: 2.193 ± 0.011	1.616PheGln: 1.616 ± 0.007	2.016PheArg: 2.016 ± 0.008	4.147PheSer: 4.147 ± 0.012	1.997PheThr: 1.997 ± 0.009	2.795PheVal: 2.795 ± 0.011	0.572PheTrp: 0.572 ± 0.005	1.302PheTyr: 1.302 ± 0.007	0.0PheXaa: 0.0 ± 0.0
Gly	3.644GlyAla: 3.644 ± 0.013	1.268GlyCys: 1.268 ± 0.007	3.237GlyAsp: 3.237 ± 0.01	3.539GlyGlu: 3.539 ± 0.011	3.038GlyPhe: 3.038 ± 0.01	4.976GlyGly: 4.976 ± 0.026	1.58GlyHis: 1.58 ± 0.007	3.578GlyIle: 3.578 ± 0.011	4.059GlyLys: 4.059 ± 0.013	5.697GlyLeu: 5.697 ± 0.017	1.466GlyMet: 1.466 ± 0.006	3.203GlyAsn: 3.203 ± 0.012	2.331GlyPro: 2.331 ± 0.008	2.075GlyGln: 2.075 ± 0.01	3.321GlyArg: 3.321 ± 0.013	5.897GlySer: 5.897 ± 0.015	3.22GlyThr: 3.22 ± 0.011	4.053GlyVal: 4.053 ± 0.012	0.866GlyTrp: 0.866 ± 0.006	2.048GlyTyr: 2.048 ± 0.011	0.0GlyXaa: 0.0 ± 0.0
His	1.565HisAla: 1.565 ± 0.007	0.548HisCys: 0.548 ± 0.005	1.255HisAsp: 1.255 ± 0.006	1.366HisGlu: 1.366 ± 0.007	1.165HisPhe: 1.165 ± 0.006	1.736HisGly: 1.736 ± 0.009	1.089HisHis: 1.089 ± 0.01	1.293HisIle: 1.293 ± 0.007	1.283HisLys: 1.283 ± 0.006	2.509HisLeu: 2.509 ± 0.009	0.606HisMet: 0.606 ± 0.004	1.152HisAsn: 1.152 ± 0.006	1.39HisPro: 1.39 ± 0.007	1.102HisGln: 1.102 ± 0.006	1.414HisArg: 1.414 ± 0.008	2.181HisSer: 2.181 ± 0.01	1.071HisThr: 1.071 ± 0.007	1.69HisVal: 1.69 ± 0.008	0.321HisTrp: 0.321 ± 0.004	0.765HisTyr: 0.765 ± 0.006	0.0HisXaa: 0.0 ± 0.0
Ile	3.529IleAla: 3.529 ± 0.012	1.133IleCys: 1.133 ± 0.006	2.924IleAsp: 2.924 ± 0.01	3.185IleGlu: 3.185 ± 0.011	2.328IlePhe: 2.328 ± 0.009	3.392IleGly: 3.392 ± 0.011	1.395IleHis: 1.395 ± 0.006	2.99IleIle: 2.99 ± 0.01	3.046IleLys: 3.046 ± 0.01	5.393IleLeu: 5.393 ± 0.016	1.203IleMet: 1.203 ± 0.007	2.386IleAsn: 2.386 ± 0.01	3.033IlePro: 3.033 ± 0.013	2.093IleGln: 2.093 ± 0.011	2.572IleArg: 2.572 ± 0.01	4.826IleSer: 4.826 ± 0.013	2.669IleThr: 2.669 ± 0.009	3.528IleVal: 3.528 ± 0.013	0.701IleTrp: 0.701 ± 0.005	1.539IleTyr: 1.539 ± 0.007	0.0IleXaa: 0.0 ± 0.0
Lys	3.898LysAla: 3.898 ± 0.013	1.018LysCys: 1.018 ± 0.006	3.377LysAsp: 3.377 ± 0.014	4.749LysGlu: 4.749 ± 0.016	2.273LysPhe: 2.273 ± 0.009	3.761LysGly: 3.761 ± 0.011	1.498LysHis: 1.498 ± 0.008	3.375LysIle: 3.375 ± 0.011	4.782LysLys: 4.782 ± 0.022	6.111LysLeu: 6.111 ± 0.018	1.485LysMet: 1.485 ± 0.008	2.866LysAsn: 2.866 ± 0.012	2.744LysPro: 2.744 ± 0.011	2.376LysGln: 2.376 ± 0.011	3.568LysArg: 3.568 ± 0.011	4.78LysSer: 4.78 ± 0.013	2.943LysThr: 2.943 ± 0.011	3.979LysVal: 3.979 ± 0.013	0.808LysTrp: 0.808 ± 0.005	1.689LysTyr: 1.689 ± 0.008	0.0LysXaa: 0.0 ± 0.0
Leu	6.341LeuAla: 6.341 ± 0.016	1.928LeuCys: 1.928 ± 0.008	5.17LeuAsp: 5.17 ± 0.014	6.311LeuGlu: 6.311 ± 0.021	3.943LeuPhe: 3.943 ± 0.015	5.594LeuGly: 5.594 ± 0.017	2.786LeuHis: 2.786 ± 0.009	4.646LeuIle: 4.646 ± 0.014	6.357LeuLys: 6.357 ± 0.019	10.06LeuLeu: 10.06 ± 0.027	2.119LeuMet: 2.119 ± 0.009	4.241LeuAsn: 4.241 ± 0.013	5.132LeuPro: 5.132 ± 0.014	4.467LeuGln: 4.467 ± 0.014	5.322LeuArg: 5.322 ± 0.014	8.825LeuSer: 8.825 ± 0.027	4.517LeuThr: 4.517 ± 0.014	6.346LeuVal: 6.346 ± 0.017	1.183LeuTrp: 1.183 ± 0.007	2.592LeuTyr: 2.592 ± 0.01	0.0LeuXaa: 0.0 ± 0.0
Met	2.006MetAla: 2.006 ± 0.008	0.355MetCys: 0.355 ± 0.003	1.404MetAsp: 1.404 ± 0.007	1.914MetGlu: 1.914 ± 0.008	0.849MetPhe: 0.849 ± 0.006	1.566MetGly: 1.566 ± 0.007	0.603MetHis: 0.603 ± 0.004	1.172MetIle: 1.172 ± 0.007	1.639MetLys: 1.639 ± 0.008	2.308MetLeu: 2.308 ± 0.009	0.682MetMet: 0.682 ± 0.006	1.062MetAsn: 1.062 ± 0.006	1.08MetPro: 1.08 ± 0.006	0.98MetGln: 0.98 ± 0.006	1.153MetArg: 1.153 ± 0.007	1.806MetSer: 1.806 ± 0.008	1.061MetThr: 1.061 ± 0.006	1.62MetVal: 1.62 ± 0.009	0.277MetTrp: 0.277 ± 0.003	0.575MetTyr: 0.575 ± 0.004	0.0MetXaa: 0.0 ± 0.0
Asn	2.81AsnAla: 2.81 ± 0.009	0.922AsnCys: 0.922 ± 0.006	2.302AsnAsp: 2.302 ± 0.011	2.716AsnGlu: 2.716 ± 0.011	2.072AsnPhe: 2.072 ± 0.01	3.273AsnGly: 3.273 ± 0.012	1.268AsnHis: 1.268 ± 0.007	2.716AsnIle: 2.716 ± 0.009	2.718AsnLys: 2.718 ± 0.011	4.906AsnLeu: 4.906 ± 0.018	1.228AsnMet: 1.228 ± 0.006	2.91AsnAsn: 2.91 ± 0.017	2.337AsnPro: 2.337 ± 0.011	1.861AsnGln: 1.861 ± 0.008	2.011AsnArg: 2.011 ± 0.008	4.201AsnSer: 4.201 ± 0.013	2.162AsnThr: 2.162 ± 0.01	2.962AsnVal: 2.962 ± 0.011	0.613AsnTrp: 0.613 ± 0.005	1.398AsnTyr: 1.398 ± 0.007	0.0AsnXaa: 0.0 ± 0.0
Pro	2.803ProAla: 2.803 ± 0.011	0.811ProCys: 0.811 ± 0.006	2.342ProAsp: 2.342 ± 0.009	2.995ProGlu: 2.995 ± 0.011	2.051ProPhe: 2.051 ± 0.009	2.533ProGly: 2.533 ± 0.01	1.215ProHis: 1.215 ± 0.007	2.366ProIle: 2.366 ± 0.01	2.782ProLys: 2.782 ± 0.01	4.44ProLeu: 4.44 ± 0.013	0.975ProMet: 0.975 ± 0.006	2.454ProAsn: 2.454 ± 0.009	3.803ProPro: 3.803 ± 0.027	1.885ProGln: 1.885 ± 0.009	2.31ProArg: 2.31 ± 0.01	5.233ProSer: 5.233 ± 0.019	2.618ProThr: 2.618 ± 0.01	2.972ProVal: 2.972 ± 0.012	0.589ProTrp: 0.589 ± 0.004	1.327ProTyr: 1.327 ± 0.007	0.0ProXaa: 0.0 ± 0.0
Gln	2.29GlnAla: 2.29 ± 0.008	0.65GlnCys: 0.65 ± 0.005	1.727GlnAsp: 1.727 ± 0.008	2.451GlnGlu: 2.451 ± 0.01	1.435GlnPhe: 1.435 ± 0.007	2.141GlnGly: 2.141 ± 0.009	1.083GlnHis: 1.083 ± 0.006	2.096GlnIle: 2.096 ± 0.009	2.485GlnLys: 2.485 ± 0.01	3.769GlnLeu: 3.769 ± 0.013	0.999GlnMet: 0.999 ± 0.006	1.999GlnAsn: 1.999 ± 0.009	1.779GlnPro: 1.779 ± 0.009	2.237GlnGln: 2.237 ± 0.019	2.142GlnArg: 2.142 ± 0.01	2.958GlnSer: 2.958 ± 0.01	1.816GlnThr: 1.816 ± 0.008	2.384GlnVal: 2.384 ± 0.009	0.472GlnTrp: 0.472 ± 0.004	0.992GlnTyr: 0.992 ± 0.007	0.0GlnXaa: 0.0 ± 0.0
Arg	2.998ArgAla: 2.998 ± 0.011	0.956ArgCys: 0.956 ± 0.006	2.629ArgAsp: 2.629 ± 0.011	3.237ArgGlu: 3.237 ± 0.012	2.14ArgPhe: 2.14 ± 0.009	3.028ArgGly: 3.028 ± 0.013	1.323ArgHis: 1.323 ± 0.007	2.824ArgIle: 2.824 ± 0.01	3.755ArgLys: 3.755 ± 0.014	4.803ArgLeu: 4.803 ± 0.013	1.284ArgMet: 1.284 ± 0.007	2.51ArgAsn: 2.51 ± 0.011	2.192ArgPro: 2.192 ± 0.01	1.803ArgGln: 1.803 ± 0.01	3.653ArgArg: 3.653 ± 0.014	4.169ArgSer: 4.169 ± 0.016	2.405ArgThr: 2.405 ± 0.01	3.248ArgVal: 3.248 ± 0.01	0.706ArgTrp: 0.706 ± 0.005	1.42ArgTyr: 1.42 ± 0.008	0.0ArgXaa: 0.0 ± 0.0
Ser	5.182SerAla: 5.182 ± 0.015	1.737SerCys: 1.737 ± 0.009	4.472SerAsp: 4.472 ± 0.013	4.876SerGlu: 4.876 ± 0.014	4.032SerPhe: 4.032 ± 0.014	5.746SerGly: 5.746 ± 0.018	2.243SerHis: 2.243 ± 0.009	4.605SerIle: 4.605 ± 0.012	5.144SerLys: 5.144 ± 0.015	8.945SerLeu: 8.945 ± 0.021	2.118SerMet: 2.118 ± 0.008	4.464SerAsn: 4.464 ± 0.016	4.541SerPro: 4.541 ± 0.018	3.222SerGln: 3.222 ± 0.01	4.302SerArg: 4.302 ± 0.015	11.05SerSer: 11.05 ± 0.032	4.693SerThr: 4.693 ± 0.013	5.299SerVal: 5.299 ± 0.015	1.15SerTrp: 1.15 ± 0.006	2.381SerTyr: 2.381 ± 0.009	0.0SerXaa: 0.0 ± 0.0
Thr	3.194ThrAla: 3.194 ± 0.011	0.989ThrCys: 0.989 ± 0.005	2.225ThrAsp: 2.225 ± 0.01	2.766ThrGlu: 2.766 ± 0.01	2.095ThrPhe: 2.095 ± 0.008	3.093ThrGly: 3.093 ± 0.011	1.132ThrHis: 1.132 ± 0.007	2.857ThrIle: 2.857 ± 0.01	2.834ThrLys: 2.834 ± 0.011	4.878ThrLeu: 4.878 ± 0.013	1.204ThrMet: 1.204 ± 0.006	2.328ThrAsn: 2.328 ± 0.01	2.628ThrPro: 2.628 ± 0.01	1.621ThrGln: 1.621 ± 0.008	2.363ThrArg: 2.363 ± 0.009	4.698ThrSer: 4.698 ± 0.013	3.163ThrThr: 3.163 ± 0.013	3.274ThrVal: 3.274 ± 0.012	0.631ThrTrp: 0.631 ± 0.005	1.401ThrTyr: 1.401 ± 0.008	0.0ThrXaa: 0.0 ± 0.0
Val	4.77ValAla: 4.77 ± 0.013	1.177ValCys: 1.177 ± 0.006	3.685ValAsp: 3.685 ± 0.012	4.317ValGlu: 4.317 ± 0.013	2.705ValPhe: 2.705 ± 0.012	4.063ValGly: 4.063 ± 0.012	1.559ValHis: 1.559 ± 0.008	3.402ValIle: 3.402 ± 0.011	3.88ValLys: 3.88 ± 0.011	6.333ValLeu: 6.333 ± 0.019	1.522ValMet: 1.522 ± 0.007	2.709ValAsn: 2.709 ± 0.01	3.203ValPro: 3.203 ± 0.012	2.348ValGln: 2.348 ± 0.009	2.962ValArg: 2.962 ± 0.009	5.475ValSer: 5.475 ± 0.016	3.271ValThr: 3.271 ± 0.011	4.949ValVal: 4.949 ± 0.014	0.752ValTrp: 0.752 ± 0.005	1.931ValTyr: 1.931 ± 0.008	0.0ValXaa: 0.0 ± 0.0
Trp	0.698TrpAla: 0.698 ± 0.005	0.251TrpCys: 0.251 ± 0.003	0.71TrpAsp: 0.71 ± 0.005	0.729TrpGlu: 0.729 ± 0.005	0.576TrpPhe: 0.576 ± 0.004	0.716TrpGly: 0.716 ± 0.006	0.316TrpHis: 0.316 ± 0.003	0.706TrpIle: 0.706 ± 0.005	0.97TrpLys: 0.97 ± 0.006	1.248TrpLeu: 1.248 ± 0.007	0.344TrpMet: 0.344 ± 0.003	0.73TrpAsn: 0.73 ± 0.006	0.499TrpPro: 0.499 ± 0.004	0.462TrpGln: 0.462 ± 0.004	0.793TrpArg: 0.793 ± 0.006	0.977TrpSer: 0.977 ± 0.007	0.622TrpThr: 0.622 ± 0.005	0.788TrpVal: 0.788 ± 0.006	0.258TrpTrp: 0.258 ± 0.003	0.348TrpTyr: 0.348 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.79TyrAla: 1.79 ± 0.009	0.651TyrCys: 0.651 ± 0.005	1.578TyrAsp: 1.578 ± 0.007	1.617TyrGlu: 1.617 ± 0.008	1.312TyrPhe: 1.312 ± 0.007	2.088TyrGly: 2.088 ± 0.009	0.769TyrHis: 0.769 ± 0.005	1.518TyrIle: 1.518 ± 0.008	1.557TyrLys: 1.557 ± 0.008	2.798TyrLeu: 2.798 ± 0.012	0.759TyrMet: 0.759 ± 0.005	1.386TyrAsn: 1.386 ± 0.008	1.303TyrPro: 1.303 ± 0.007	0.974TyrGln: 0.974 ± 0.006	1.428TyrArg: 1.428 ± 0.007	2.276TyrSer: 2.276 ± 0.01	1.294TyrThr: 1.294 ± 0.008	1.788TyrVal: 1.788 ± 0.007	0.409TyrTrp: 0.409 ± 0.004	0.976TyrTyr: 0.976 ± 0.007	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.01XaaXaa: 0.01 ± 0.005

Statistics based on 74868 proteins (30697910 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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