Chlamydia psittaci 84-8471/1
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2863 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A656K840|A0A656K840_CHLPS Uncharacterized protein (Fragment) OS=Chlamydia psittaci 84-8471/1 OX=1112230 GN=CP8484711_0677 PE=4 SV=1
MM1 pKa = 7.43 SVTDD5 pKa = 3.91 PDD7 pKa = 3.77 FFLRR11 pKa = 11.84 QAADD15 pKa = 3.48 YY16 pKa = 10.5 LDD18 pKa = 3.61 QGKK21 pKa = 10.46 VIAFPTDD28 pKa = 3.02 TVYY31 pKa = 11.44 GLGVALNYY39 pKa = 10.16 PNAEE43 pKa = 3.99 EE44 pKa = 5.17 KK45 pKa = 10.35 IYY47 pKa = 11.02 DD48 pKa = 3.69 LL49 pKa = 5.17
Molecular weight: 5.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.923
IPC_protein 3.795
Toseland 3.592
ProMoST 4.012
Dawson 3.808
Bjellqvist 3.986
Wikipedia 3.808
Rodwell 3.63
Grimsley 3.516
Solomon 3.783
Lehninger 3.732
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.923
Patrickios 1.926
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A656KB93|A0A656KB93_CHLPS tRNA(Ile)-lysidine synthase domain protein (Fragment) OS=Chlamydia psittaci 84-8471/1 OX=1112230 GN=CP8484711_1809 PE=4 SV=1
TT1 pKa = 6.11 TARR4 pKa = 11.84 KK5 pKa = 7.09 TVRR8 pKa = 11.84 KK9 pKa = 5.43 TTVRR13 pKa = 11.84 KK14 pKa = 7.55 TVAKK18 pKa = 8.89 KK19 pKa = 6.79 TTAKK23 pKa = 10.26 KK24 pKa = 10.2 AATRR28 pKa = 11.84 KK29 pKa = 8.66 PAVKK33 pKa = 10.11 KK34 pKa = 10.49 AVRR37 pKa = 11.84 KK38 pKa = 7.14 TAAKK42 pKa = 8.02 KK43 pKa = 6.3 TTVRR47 pKa = 11.84 KK48 pKa = 7.42 TVRR51 pKa = 11.84 KK52 pKa = 5.03 TTARR56 pKa = 11.84 KK57 pKa = 7.26 TVANKK62 pKa = 8.51 ATAKK66 pKa = 9.11 KK67 pKa = 8.17 TATRR71 pKa = 3.75
Molecular weight: 7.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.496
IPC2_protein 11.228
IPC_protein 12.749
Toseland 12.939
ProMoST 13.408
Dawson 12.939
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.925
Grimsley 12.969
Solomon 13.408
Lehninger 13.32
Nozaki 12.925
DTASelect 12.91
Thurlkill 12.925
EMBOSS 13.422
Sillero 12.925
Patrickios 12.647
IPC_peptide 13.422
IPC2_peptide 12.398
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2863
0
2863
285655
8
932
99.8
11.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.581 ± 0.056
1.715 ± 0.032
4.496 ± 0.05
6.239 ± 0.072
4.98 ± 0.066
5.881 ± 0.069
2.501 ± 0.034
7.164 ± 0.057
6.348 ± 0.06
11.024 ± 0.083
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.251 ± 0.033
4.151 ± 0.049
4.396 ± 0.05
3.869 ± 0.049
4.651 ± 0.053
8.277 ± 0.065
5.195 ± 0.052
6.065 ± 0.062
0.953 ± 0.022
3.263 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here