Mojiang virus
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8TIR0|W8TIR0_9MONO Isoform of W8SKS6 V protein OS=Mojiang virus OX=1474807 GN=C PE=4 SV=1
MM1 pKa = 7.94 SYY3 pKa = 10.83 EE4 pKa = 4.27 DD5 pKa = 5.22 RR6 pKa = 11.84 IKK8 pKa = 10.62 QIQNGLQIVDD18 pKa = 3.8 LVKK21 pKa = 10.46 KK22 pKa = 10.17 VRR24 pKa = 11.84 QEE26 pKa = 4.24 SIEE29 pKa = 4.0 KK30 pKa = 8.15 PTYY33 pKa = 8.89 GRR35 pKa = 11.84 SAIGLPTTKK44 pKa = 10.36 DD45 pKa = 2.84 RR46 pKa = 11.84 AAAWEE51 pKa = 4.32 LFHH54 pKa = 7.56 KK55 pKa = 10.8 SSLDD59 pKa = 3.39 EE60 pKa = 4.88 AGPEE64 pKa = 3.85 EE65 pKa = 4.91 LPLKK69 pKa = 10.66 EE70 pKa = 4.89 GDD72 pKa = 4.36 DD73 pKa = 4.04 PADD76 pKa = 3.45 TRR78 pKa = 11.84 DD79 pKa = 3.49 GVGIPEE85 pKa = 4.5 PLHH88 pKa = 6.47 NVDD91 pKa = 3.64 SGGSRR96 pKa = 11.84 TYY98 pKa = 11.05 KK99 pKa = 8.96 EE100 pKa = 4.56 ANWDD104 pKa = 3.72 EE105 pKa = 4.07 GDD107 pKa = 3.85 EE108 pKa = 4.87 PILEE112 pKa = 4.33 NQLVTNIQPNDD123 pKa = 3.92 PGRR126 pKa = 11.84 KK127 pKa = 5.62 TAYY130 pKa = 9.7 GKK132 pKa = 10.28 SDD134 pKa = 3.17 HH135 pKa = 6.92 TYY137 pKa = 11.49 ADD139 pKa = 3.45 STNNRR144 pKa = 11.84 TKK146 pKa = 10.75 GGEE149 pKa = 3.8 WSNGCSSPNKK159 pKa = 9.55 VDD161 pKa = 3.16 VSGIFQGDD169 pKa = 3.67 VPKK172 pKa = 10.37 DD173 pKa = 3.31 TDD175 pKa = 3.75 AEE177 pKa = 4.74 GPSEE181 pKa = 4.12 KK182 pKa = 9.84 PKK184 pKa = 10.97 SSFRR188 pKa = 11.84 MNPNAQEE195 pKa = 4.09 YY196 pKa = 9.81 IPRR199 pKa = 11.84 DD200 pKa = 3.77 LTPLTVIIDD209 pKa = 3.84 SCPFDD214 pKa = 5.0 HH215 pKa = 6.99 NHH217 pKa = 6.99 ADD219 pKa = 5.2 DD220 pKa = 5.03 DD221 pKa = 5.42 SEE223 pKa = 4.91 DD224 pKa = 3.61 QTEE227 pKa = 3.97 DD228 pKa = 2.94 SAYY231 pKa = 10.75 VFEE234 pKa = 6.11 AGLNKK239 pKa = 9.88 PAVKK243 pKa = 9.96 PRR245 pKa = 11.84 MIKK248 pKa = 10.15 AAQKK252 pKa = 7.82 EE253 pKa = 4.47 TLVDD257 pKa = 3.39 QDD259 pKa = 4.2 GEE261 pKa = 4.54 VMNLSILPKK270 pKa = 9.41 QRR272 pKa = 11.84 KK273 pKa = 9.37 SILNKK278 pKa = 10.28 PIGAEE283 pKa = 3.65 DD284 pKa = 5.6 AIPKK288 pKa = 9.16 KK289 pKa = 8.14 QARR292 pKa = 11.84 PSLVVIEE299 pKa = 5.24 EE300 pKa = 4.17 DD301 pKa = 5.71 DD302 pKa = 3.96 EE303 pKa = 4.45 DD304 pKa = 4.07 QKK306 pKa = 11.69 SEE308 pKa = 4.43 PIEE311 pKa = 4.86 NIDD314 pKa = 4.48 KK315 pKa = 10.96 SDD317 pKa = 3.67 AGSDD321 pKa = 3.02 ITIFDD326 pKa = 3.99 IADD329 pKa = 3.57 KK330 pKa = 10.46 ATDD333 pKa = 3.43 HH334 pKa = 6.85 LRR336 pKa = 11.84 RR337 pKa = 11.84 NQMAVKK343 pKa = 10.32 AIGNIIEE350 pKa = 4.29 TSAGVPIIQEE360 pKa = 3.97 EE361 pKa = 4.6 VIYY364 pKa = 10.88 LSDD367 pKa = 4.97 RR368 pKa = 11.84 PTQPAPEE375 pKa = 5.01 KK376 pKa = 10.53 IPAKK380 pKa = 9.94 EE381 pKa = 3.98 SRR383 pKa = 11.84 KK384 pKa = 9.81 LRR386 pKa = 11.84 ALEE389 pKa = 3.87 KK390 pKa = 9.2 TEE392 pKa = 4.05 SGKK395 pKa = 11.21 GEE397 pKa = 4.13 SDD399 pKa = 3.02 SGLRR403 pKa = 11.84 LVKK406 pKa = 10.14 KK407 pKa = 8.05 GHH409 pKa = 5.47 RR410 pKa = 11.84 RR411 pKa = 11.84 EE412 pKa = 5.01 YY413 pKa = 10.01 SLCWDD418 pKa = 3.3 GSEE421 pKa = 3.92 IKK423 pKa = 10.65 VEE425 pKa = 4.33 EE426 pKa = 4.33 WCNPICSKK434 pKa = 10.85 VKK436 pKa = 10.51 SEE438 pKa = 4.12 PSRR441 pKa = 11.84 EE442 pKa = 3.86 KK443 pKa = 10.09 CTCKK447 pKa = 9.98 QCPIMCQDD455 pKa = 3.29 EE456 pKa = 4.68 HH457 pKa = 5.98 CTKK460 pKa = 10.17 IEE462 pKa = 4.01 YY463 pKa = 8.82 EE464 pKa = 4.05
Molecular weight: 51.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.897
IPC2_protein 4.863
IPC_protein 4.813
Toseland 4.685
ProMoST 4.902
Dawson 4.774
Bjellqvist 4.927
Wikipedia 4.635
Rodwell 4.673
Grimsley 4.596
Solomon 4.762
Lehninger 4.724
Nozaki 4.876
DTASelect 5.041
Thurlkill 4.673
EMBOSS 4.66
Sillero 4.94
Patrickios 4.202
IPC_peptide 4.774
IPC2_peptide 4.94
IPC2.peptide.svr19 4.892
Protein with the highest isoelectric point:
>tr|W8SYF7|W8SYF7_9MONO Isoform of W8SKS6 W protein OS=Mojiang virus OX=1474807 GN=C PE=4 SV=1
MM1 pKa = 7.99 ASRR4 pKa = 11.84 LLTWSRR10 pKa = 11.84 KK11 pKa = 8.83 LGRR14 pKa = 11.84 NRR16 pKa = 11.84 SKK18 pKa = 10.57 SQHH21 pKa = 5.06 TGEE24 pKa = 4.19 VQLAFQQPKK33 pKa = 9.84 IEE35 pKa = 4.03 QQPGSCSINLHH46 pKa = 5.79 LMKK49 pKa = 10.05 PDD51 pKa = 4.7 LKK53 pKa = 11.14 NCLLKK58 pKa = 10.74 KK59 pKa = 10.66 EE60 pKa = 4.2 MTQLTLEE67 pKa = 4.41 MEE69 pKa = 4.32 WAFQSLFIMLTVEE82 pKa = 4.17 EE83 pKa = 4.68 AEE85 pKa = 4.1 HH86 pKa = 6.09 TKK88 pKa = 10.52 RR89 pKa = 11.84 QTGMRR94 pKa = 11.84 EE95 pKa = 3.66 TNQYY99 pKa = 11.34 SKK101 pKa = 10.77 ISWLQTFNRR110 pKa = 11.84 MIQEE114 pKa = 3.89 GRR116 pKa = 11.84 LPTGNLITRR125 pKa = 11.84 MLTAQIIEE133 pKa = 4.23 PKK135 pKa = 9.23 EE136 pKa = 3.72 VSGAMDD142 pKa = 3.51 VLRR145 pKa = 11.84 LIRR148 pKa = 11.84 LMCPAYY154 pKa = 10.5 SRR156 pKa = 11.84 AMYY159 pKa = 10.55 LRR161 pKa = 11.84 TQMLKK166 pKa = 10.86 DD167 pKa = 3.54 LVRR170 pKa = 11.84 NPSHH174 pKa = 6.81 HH175 pKa = 6.86 SEE177 pKa = 3.97
Molecular weight: 20.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.065
IPC2_protein 9.048
IPC_protein 9.063
Toseland 9.984
ProMoST 9.575
Dawson 10.131
Bjellqvist 9.794
Wikipedia 10.262
Rodwell 10.584
Grimsley 10.175
Solomon 10.175
Lehninger 10.16
Nozaki 10.043
DTASelect 9.765
Thurlkill 10.014
EMBOSS 10.379
Sillero 10.072
Patrickios 10.321
IPC_peptide 10.175
IPC2_peptide 8.478
IPC2.peptide.svr19 8.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
3
9
6095
177
2277
677.2
76.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.595 ± 0.708
1.575 ± 0.318
6.497 ± 0.53
6.202 ± 0.634
2.97 ± 0.426
5.628 ± 0.46
1.854 ± 0.337
8.236 ± 0.47
6.776 ± 0.45
9.04 ± 0.734
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.592 ± 0.331
5.742 ± 0.271
4.742 ± 0.44
3.741 ± 0.384
4.791 ± 0.369
8.023 ± 0.273
5.89 ± 0.381
5.513 ± 0.375
1.017 ± 0.155
3.577 ± 0.509
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here