Yunnan orbivirus
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2YH50|Q2YH50_9REOV Non-structural protein NS2 OS=Yunnan orbivirus OX=306276 PE=4 SV=1
MM1 pKa = 7.29 EE2 pKa = 6.13 GIYY5 pKa = 10.7 ARR7 pKa = 11.84 AFCYY11 pKa = 10.48 LEE13 pKa = 4.37 CLASNRR19 pKa = 11.84 DD20 pKa = 3.11 PRR22 pKa = 11.84 ARR24 pKa = 11.84 RR25 pKa = 11.84 TFQPSDD31 pKa = 3.06 GFALFVTRR39 pKa = 11.84 FNATTDD45 pKa = 3.48 RR46 pKa = 11.84 PISQTPSTQDD56 pKa = 2.9 EE57 pKa = 4.47 HH58 pKa = 7.26 RR59 pKa = 11.84 SCFYY63 pKa = 11.26 AGLDD67 pKa = 3.7 LVLGALGINFNYY79 pKa = 10.03 NLPGYY84 pKa = 7.78 TPNVQILSILARR96 pKa = 11.84 DD97 pKa = 3.85 DD98 pKa = 4.38 LPYY101 pKa = 10.48 TSEE104 pKa = 4.35 AYY106 pKa = 10.33 VRR108 pKa = 11.84 ASRR111 pKa = 11.84 IINEE115 pKa = 4.14 PGAGQSTRR123 pKa = 11.84 HH124 pKa = 5.36 FTLPWPDD131 pKa = 3.19 VKK133 pKa = 10.62 FLYY136 pKa = 10.18 PPATPYY142 pKa = 9.15 RR143 pKa = 11.84 TAADD147 pKa = 4.27 EE148 pKa = 4.3 PQPMAVGAQDD158 pKa = 3.55 MEE160 pKa = 4.72 VTLVPAEE167 pKa = 3.98 EE168 pKa = 4.38 LEE170 pKa = 4.13 ITEE173 pKa = 4.57 TVMPNYY179 pKa = 9.57 PDD181 pKa = 4.21 VINACIIWYY190 pKa = 7.39 TLSHH194 pKa = 5.54 YY195 pKa = 10.12 RR196 pKa = 11.84 GPGGALIEE204 pKa = 5.01 GSDD207 pKa = 3.77 TCQLTIDD214 pKa = 4.35 DD215 pKa = 4.84 EE216 pKa = 4.42 EE217 pKa = 5.06 VEE219 pKa = 4.42 AGVEE223 pKa = 4.24 LIVPVGSPIRR233 pKa = 11.84 VINSSAVNTGIIHH246 pKa = 6.08 ITVKK250 pKa = 9.7 WFTRR254 pKa = 11.84 AQPSDD259 pKa = 3.85 DD260 pKa = 5.27 LYY262 pKa = 10.99 DD263 pKa = 3.58 TMRR266 pKa = 11.84 VDD268 pKa = 3.62 IGASYY273 pKa = 10.54 SYY275 pKa = 10.89 HH276 pKa = 6.03 DD277 pKa = 3.97 QQWYY281 pKa = 9.91 DD282 pKa = 3.38 LRR284 pKa = 11.84 SYY286 pKa = 11.17 LLRR289 pKa = 11.84 QMDD292 pKa = 4.14 IPPNVPPNEE301 pKa = 4.65 PIRR304 pKa = 11.84 VANRR308 pKa = 11.84 ILALALVSRR317 pKa = 11.84 LQNAYY322 pKa = 9.53 VAHH325 pKa = 6.6 SPMNLQAPQNLNINGQIGVPNAVLDD350 pKa = 4.04 ALRR353 pKa = 11.84 RR354 pKa = 11.84 AA355 pKa = 4.04
Molecular weight: 39.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.666
IPC2_protein 4.825
IPC_protein 4.762
Toseland 4.635
ProMoST 4.902
Dawson 4.736
Bjellqvist 4.876
Wikipedia 4.635
Rodwell 4.635
Grimsley 4.546
Solomon 4.736
Lehninger 4.698
Nozaki 4.851
DTASelect 5.041
Thurlkill 4.647
EMBOSS 4.66
Sillero 4.914
Patrickios 3.249
IPC_peptide 4.749
IPC2_peptide 4.902
IPC2.peptide.svr19 4.867
Protein with the highest isoelectric point:
>tr|Q2YH48|Q2YH48_9REOV VP6 OS=Yunnan orbivirus OX=306276 PE=4 SV=1
MM1 pKa = 7.23 HH2 pKa = 7.51 AAITSMKK9 pKa = 9.86 RR10 pKa = 11.84 EE11 pKa = 4.3 HH12 pKa = 7.11 PSHH15 pKa = 6.75 LLAVVPASAPRR26 pKa = 11.84 YY27 pKa = 7.76 EE28 pKa = 4.26 EE29 pKa = 4.89 GYY31 pKa = 8.43 NTEE34 pKa = 4.13 NEE36 pKa = 4.12 KK37 pKa = 8.33 THH39 pKa = 5.97 QKK41 pKa = 10.84 EE42 pKa = 3.96 NLLDD46 pKa = 3.9 LSLPGKK52 pKa = 9.32 MATKK56 pKa = 10.83 AMALDD61 pKa = 3.67 VLTTTFGGPHH71 pKa = 6.7 GSDD74 pKa = 3.83 EE75 pKa = 3.99 IQKK78 pKa = 9.62 RR79 pKa = 11.84 EE80 pKa = 3.71 KK81 pKa = 10.11 GAYY84 pKa = 8.45 VAATQALNEE93 pKa = 4.35 DD94 pKa = 3.29 KK95 pKa = 10.36 GTRR98 pKa = 11.84 KK99 pKa = 9.7 LRR101 pKa = 11.84 VYY103 pKa = 9.65 MNHH106 pKa = 6.31 QILPRR111 pKa = 11.84 LQGKK115 pKa = 9.0 LRR117 pKa = 11.84 TASIKK122 pKa = 9.89 YY123 pKa = 9.67 RR124 pKa = 11.84 LWLTIEE130 pKa = 4.65 IIAALLSITVFGIMATTEE148 pKa = 3.84 MEE150 pKa = 4.23 QTVDD154 pKa = 2.59 TWLKK158 pKa = 8.69 EE159 pKa = 3.83 KK160 pKa = 10.75 FGIRR164 pKa = 11.84 MGMSIVSMLATVLLLISSRR183 pKa = 11.84 MAGGLRR189 pKa = 11.84 EE190 pKa = 4.03 SMKK193 pKa = 9.96 RR194 pKa = 11.84 LKK196 pKa = 10.85 RR197 pKa = 11.84 EE198 pKa = 3.65 VEE200 pKa = 3.7 KK201 pKa = 10.9 RR202 pKa = 11.84 KK203 pKa = 8.04 TYY205 pKa = 10.67 NYY207 pKa = 9.94 VSEE210 pKa = 4.24 EE211 pKa = 3.95 MALEE215 pKa = 4.34 TEE217 pKa = 4.39 THH219 pKa = 5.35 GKK221 pKa = 9.68 EE222 pKa = 3.71 MEE224 pKa = 4.24 TRR226 pKa = 11.84 LSSSKK231 pKa = 10.44 YY232 pKa = 9.27 PVWFDD237 pKa = 3.4 ATGKK241 pKa = 10.44 AATAGGWQLMQVV253 pKa = 3.53
Molecular weight: 28.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.862
IPC2_protein 8.814
IPC_protein 8.741
Toseland 9.56
ProMoST 9.253
Dawson 9.794
Bjellqvist 9.428
Wikipedia 9.955
Rodwell 10.175
Grimsley 9.867
Solomon 9.838
Lehninger 9.794
Nozaki 9.516
DTASelect 9.443
Thurlkill 9.633
EMBOSS 9.97
Sillero 9.692
Patrickios 5.652
IPC_peptide 9.823
IPC2_peptide 7.849
IPC2.peptide.svr19 7.907
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
6263
253
1315
626.3
71.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.419 ± 0.557
1.293 ± 0.213
5.668 ± 0.417
6.355 ± 0.433
3.992 ± 0.404
5.173 ± 0.349
2.315 ± 0.272
6.227 ± 0.287
6.099 ± 0.516
9.708 ± 0.481
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.082 ± 0.189
4.183 ± 0.253
4.327 ± 0.325
4.135 ± 0.219
6.179 ± 0.158
6.962 ± 0.229
6.722 ± 0.427
6.546 ± 0.224
1.038 ± 0.199
3.577 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here