Denitratisoma sp. DHT3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Sterolibacteriaceae; Denitratisoma; unclassified Denitratisoma

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3216 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A518UC59|A0A518UC59_9PROT 4Fe-4S Mo/W bis-MGD-type domain-containing protein OS=Denitratisoma sp. DHT3 OX=1981880 GN=B9N43_14050 PE=3 SV=1
MM1 pKa = 7.36NAPTEE6 pKa = 4.22MPGPFVFTDD15 pKa = 3.49SAVSKK20 pKa = 10.34VKK22 pKa = 10.41EE23 pKa = 4.22LIAEE27 pKa = 4.3EE28 pKa = 4.52GNPDD32 pKa = 2.99LKK34 pKa = 11.14LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.9GFTFDD54 pKa = 4.44EE55 pKa = 4.63VANDD59 pKa = 4.79DD60 pKa = 4.2DD61 pKa = 3.8TSMIKK66 pKa = 10.63DD67 pKa = 3.67GVTLLIDD74 pKa = 3.72PMSYY78 pKa = 10.31QYY80 pKa = 11.58LVGAEE85 pKa = 3.64IDD87 pKa = 3.75YY88 pKa = 10.77TEE90 pKa = 4.43GLEE93 pKa = 4.39GSQFVIKK100 pKa = 10.61NPNANSTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A518UBL0|A0A518UBL0_9PROT MerR family transcriptional regulator OS=Denitratisoma sp. DHT3 OX=1981880 GN=B9N43_12925 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.6RR14 pKa = 11.84THH16 pKa = 5.72GFLVRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.82GRR39 pKa = 11.84HH40 pKa = 5.12RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3216

0

3216

1081138

30

2445

336.2

36.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.209 ± 0.063

1.018 ± 0.017

5.435 ± 0.032

5.828 ± 0.038

3.518 ± 0.029

8.415 ± 0.051

2.272 ± 0.018

4.706 ± 0.032

3.419 ± 0.043

10.852 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.021

2.768 ± 0.027

5.146 ± 0.03

3.705 ± 0.025

7.307 ± 0.047

5.164 ± 0.035

4.956 ± 0.042

7.137 ± 0.034

1.367 ± 0.017

2.39 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski