uncultured phage_MedDCM-OCT-S35-C6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pelagivirus; Pelagivirus S35C6

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4P9S3|A0A6S4P9S3_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S35-C6 OX=2741075 PE=4 SV=1
MM1 pKa = 7.29AQDD4 pKa = 4.1LSEE7 pKa = 6.21KK8 pKa = 10.15IDD10 pKa = 4.12DD11 pKa = 4.52YY12 pKa = 11.79CNDD15 pKa = 3.8MYY17 pKa = 11.81GHH19 pKa = 6.21TNWGYY24 pKa = 10.66LDD26 pKa = 3.96TYY28 pKa = 11.18SKK30 pKa = 11.48EE31 pKa = 3.9EE32 pKa = 4.46LDD34 pKa = 5.29AKK36 pKa = 11.14DD37 pKa = 5.2HH38 pKa = 7.06DD39 pKa = 3.96IEE41 pKa = 5.15DD42 pKa = 4.53NIVYY46 pKa = 8.79WHH48 pKa = 7.06EE49 pKa = 3.81EE50 pKa = 3.93AEE52 pKa = 4.42DD53 pKa = 4.08DD54 pKa = 4.8EE55 pKa = 4.58EE56 pKa = 4.51CKK58 pKa = 10.62PIITISDD65 pKa = 4.39EE66 pKa = 4.04IQKK69 pKa = 7.32TTEE72 pKa = 3.68VV73 pKa = 3.43

Molecular weight: 8.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4P8H4|A0A6S4P8H4_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S35-C6 OX=2741075 PE=4 SV=1
MM1 pKa = 7.07YY2 pKa = 7.74ATRR5 pKa = 11.84RR6 pKa = 11.84TSMIIYY12 pKa = 9.27EE13 pKa = 4.32YY14 pKa = 10.38TPKK17 pKa = 9.71TWLNKK22 pKa = 9.61VKK24 pKa = 10.28IYY26 pKa = 8.81WQNTNKK32 pKa = 10.48KK33 pKa = 9.2LFTAFVVWSVILWVMM48 pKa = 4.37

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12637

39

1264

229.8

25.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.328 ± 0.373

0.752 ± 0.13

5.951 ± 0.219

6.267 ± 0.478

3.87 ± 0.218

6.513 ± 0.322

1.733 ± 0.149

6.647 ± 0.175

8.174 ± 0.58

7.874 ± 0.376

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.754 ± 0.21

6.441 ± 0.337

2.944 ± 0.151

3.695 ± 0.246

3.767 ± 0.294

7.478 ± 0.546

7.423 ± 0.64

5.571 ± 0.32

1.25 ± 0.165

3.569 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski