Croceibacter phage P2559S
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6R1F2|I6R1F2_9CAUD Uncharacterized protein OS=Croceibacter phage P2559S OX=1176422 GN=P2559S_43 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 9.98 FYY4 pKa = 10.42 ATIRR8 pKa = 11.84 IEE10 pKa = 4.87 LPCEE14 pKa = 4.13 PNEE17 pKa = 4.1 KK18 pKa = 10.27 AAVEE22 pKa = 4.07 MAQLATAQLDD32 pKa = 3.74 NLDD35 pKa = 4.09 SVEE38 pKa = 4.43 NPYY41 pKa = 10.88 LCYY44 pKa = 9.57 VTKK47 pKa = 10.45 QDD49 pKa = 3.71 GLNVTEE55 pKa = 4.17 VLNNIDD61 pKa = 3.15
Molecular weight: 6.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.889
IPC2_protein 4.202
IPC_protein 4.024
Toseland 3.859
ProMoST 4.037
Dawson 3.986
Bjellqvist 4.228
Wikipedia 3.884
Rodwell 3.859
Grimsley 3.77
Solomon 3.961
Lehninger 3.91
Nozaki 4.113
DTASelect 4.24
Thurlkill 3.897
EMBOSS 3.897
Sillero 4.139
Patrickios 1.926
IPC_peptide 3.961
IPC2_peptide 4.113
IPC2.peptide.svr19 4.085
Protein with the highest isoelectric point:
>tr|I6S6X7|I6S6X7_9CAUD Uncharacterized protein OS=Croceibacter phage P2559S OX=1176422 GN=P2559S_12 PE=4 SV=1
MM1 pKa = 6.98 ATEE4 pKa = 3.96 TRR6 pKa = 11.84 VRR8 pKa = 11.84 RR9 pKa = 11.84 LKK11 pKa = 10.63 AQVHH15 pKa = 5.93 RR16 pKa = 11.84 LHH18 pKa = 6.29 GTKK21 pKa = 10.1 EE22 pKa = 3.84 MRR24 pKa = 11.84 AEE26 pKa = 4.0 QAKK29 pKa = 10.91 GEE31 pKa = 4.14 ATEE34 pKa = 4.58 KK35 pKa = 10.93 ALQAYY40 pKa = 8.97 YY41 pKa = 10.67 DD42 pKa = 4.37 AYY44 pKa = 11.21 DD45 pKa = 3.94 KK46 pKa = 11.31 FDD48 pKa = 4.67 DD49 pKa = 4.79 ALSDD53 pKa = 3.72 YY54 pKa = 10.68 YY55 pKa = 11.05 AAKK58 pKa = 10.41 LMLMKK63 pKa = 10.54 VKK65 pKa = 10.73 NPDD68 pKa = 2.89 IFEE71 pKa = 4.45 MYY73 pKa = 10.67 LGLTKK78 pKa = 10.27 QAAKK82 pKa = 10.64 SKK84 pKa = 8.71 MLRR87 pKa = 11.84 QITRR91 pKa = 11.84 LKK93 pKa = 10.81 NSNLSNQLNVNRR105 pKa = 11.84 INKK108 pKa = 9.38 LKK110 pKa = 10.58 EE111 pKa = 3.99 AIKK114 pKa = 10.6 NNKK117 pKa = 9.07 NKK119 pKa = 10.01 AQKK122 pKa = 10.46 AKK124 pKa = 10.07 LRR126 pKa = 11.84 KK127 pKa = 9.06 VLGRR131 pKa = 11.84 LEE133 pKa = 4.38 SWAKK137 pKa = 9.98 DD138 pKa = 3.28 LNYY141 pKa = 10.48 EE142 pKa = 3.99 EE143 pKa = 5.41 RR144 pKa = 11.84 QGKK147 pKa = 7.28 TYY149 pKa = 11.11 YY150 pKa = 10.28 KK151 pKa = 10.73 AQGG154 pKa = 3.16
Molecular weight: 17.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.101
IPC2_protein 9.458
IPC_protein 9.414
Toseland 10.233
ProMoST 9.809
Dawson 10.379
Bjellqvist 9.999
Wikipedia 10.511
Rodwell 10.979
Grimsley 10.423
Solomon 10.394
Lehninger 10.365
Nozaki 10.189
DTASelect 9.999
Thurlkill 10.233
EMBOSS 10.613
Sillero 10.277
Patrickios 10.672
IPC_peptide 10.394
IPC2_peptide 8.419
IPC2.peptide.svr19 8.537
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
0
68
14262
39
1257
209.7
23.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.274 ± 0.534
0.89 ± 0.162
6.135 ± 0.163
6.963 ± 0.36
3.976 ± 0.246
6.5 ± 0.288
1.571 ± 0.185
6.275 ± 0.199
8.54 ± 0.494
8.26 ± 0.243
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.3 ± 0.191
5.637 ± 0.336
3.169 ± 0.246
4.859 ± 0.225
4.095 ± 0.2
5.238 ± 0.201
6.065 ± 0.263
5.862 ± 0.244
1.297 ± 0.123
4.095 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here