Amino acid dipepetide frequency for Saccharothrix sp. CB00851

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.304AlaAla: 19.304 ± 0.14	0.979AlaCys: 0.979 ± 0.021	8.599AlaAsp: 8.599 ± 0.058	8.704AlaGlu: 8.704 ± 0.079	3.641AlaPhe: 3.641 ± 0.039	12.075AlaGly: 12.075 ± 0.075	2.873AlaHis: 2.873 ± 0.04	3.716AlaIle: 3.716 ± 0.042	2.752AlaLys: 2.752 ± 0.043	14.385AlaLeu: 14.385 ± 0.107	2.337AlaMet: 2.337 ± 0.032	2.314AlaAsn: 2.314 ± 0.032	5.984AlaPro: 5.984 ± 0.066	3.425AlaGln: 3.425 ± 0.043	9.934AlaArg: 9.934 ± 0.08	5.664AlaSer: 5.664 ± 0.05	7.115AlaThr: 7.115 ± 0.061	13.27AlaVal: 13.27 ± 0.099	1.968AlaTrp: 1.968 ± 0.029	2.371AlaTyr: 2.371 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	1.02CysAla: 1.02 ± 0.021	0.084CysCys: 0.084 ± 0.006	0.452CysAsp: 0.452 ± 0.015	0.367CysGlu: 0.367 ± 0.013	0.211CysPhe: 0.211 ± 0.01	0.898CysGly: 0.898 ± 0.019	0.167CysHis: 0.167 ± 0.009	0.12CysIle: 0.12 ± 0.007	0.101CysLys: 0.101 ± 0.007	0.71CysLeu: 0.71 ± 0.017	0.098CysMet: 0.098 ± 0.006	0.125CysAsn: 0.125 ± 0.008	0.442CysPro: 0.442 ± 0.014	0.165CysGln: 0.165 ± 0.009	0.579CysArg: 0.579 ± 0.016	0.402CysSer: 0.402 ± 0.014	0.492CysThr: 0.492 ± 0.014	0.689CysVal: 0.689 ± 0.017	0.126CysTrp: 0.126 ± 0.007	0.17CysTyr: 0.17 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.589AspAla: 7.589 ± 0.056	0.406AspCys: 0.406 ± 0.015	4.164AspAsp: 4.164 ± 0.039	4.044AspGlu: 4.044 ± 0.042	1.699AspPhe: 1.699 ± 0.027	6.287AspGly: 6.287 ± 0.053	1.686AspHis: 1.686 ± 0.028	1.58AspIle: 1.58 ± 0.03	1.005AspLys: 1.005 ± 0.025	7.091AspLeu: 7.091 ± 0.06	0.684AspMet: 0.684 ± 0.016	1.108AspAsn: 1.108 ± 0.021	4.612AspPro: 4.612 ± 0.042	1.817AspGln: 1.817 ± 0.03	5.541AspArg: 5.541 ± 0.057	2.415AspSer: 2.415 ± 0.029	3.021AspThr: 3.021 ± 0.033	6.173AspVal: 6.173 ± 0.053	1.014AspTrp: 1.014 ± 0.019	1.273AspTyr: 1.273 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	6.77GluAla: 6.77 ± 0.068	0.36GluCys: 0.36 ± 0.013	2.73GluAsp: 2.73 ± 0.036	2.928GluGlu: 2.928 ± 0.04	1.718GluPhe: 1.718 ± 0.028	3.647GluGly: 3.647 ± 0.043	1.801GluHis: 1.801 ± 0.029	1.897GluIle: 1.897 ± 0.031	1.072GluLys: 1.072 ± 0.024	7.118GluLeu: 7.118 ± 0.064	0.795GluMet: 0.795 ± 0.016	0.946GluAsn: 0.946 ± 0.022	3.499GluPro: 3.499 ± 0.042	2.215GluGln: 2.215 ± 0.031	5.207GluArg: 5.207 ± 0.056	2.315GluSer: 2.315 ± 0.033	2.399GluThr: 2.399 ± 0.034	5.764GluVal: 5.764 ± 0.051	0.845GluTrp: 0.845 ± 0.017	1.023GluTyr: 1.023 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.953PheAla: 3.953 ± 0.042	0.245PheCys: 0.245 ± 0.009	2.262PheAsp: 2.262 ± 0.035	1.479PheGlu: 1.479 ± 0.025	0.837PhePhe: 0.837 ± 0.022	3.267PheGly: 3.267 ± 0.039	0.646PheHis: 0.646 ± 0.016	0.677PheIle: 0.677 ± 0.016	0.462PheLys: 0.462 ± 0.015	2.653PheLeu: 2.653 ± 0.034	0.339PheMet: 0.339 ± 0.012	0.591PheAsn: 0.591 ± 0.015	1.396PhePro: 1.396 ± 0.025	0.695PheGln: 0.695 ± 0.017	1.965PheArg: 1.965 ± 0.028	1.438PheSer: 1.438 ± 0.024	2.262PheThr: 2.262 ± 0.029	2.468PheVal: 2.468 ± 0.034	0.42PheTrp: 0.42 ± 0.012	0.593PheTyr: 0.593 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	9.94GlyAla: 9.94 ± 0.069	0.752GlyCys: 0.752 ± 0.018	5.27GlyAsp: 5.27 ± 0.049	5.027GlyGlu: 5.027 ± 0.053	2.974GlyPhe: 2.974 ± 0.037	8.391GlyGly: 8.391 ± 0.09	2.189GlyHis: 2.189 ± 0.027	2.999GlyIle: 2.999 ± 0.039	2.23GlyLys: 2.23 ± 0.04	9.266GlyLeu: 9.266 ± 0.079	1.793GlyMet: 1.793 ± 0.028	1.88GlyAsn: 1.88 ± 0.038	4.688GlyPro: 4.688 ± 0.044	2.61GlyGln: 2.61 ± 0.038	7.236GlyArg: 7.236 ± 0.06	4.878GlySer: 4.878 ± 0.052	5.938GlyThr: 5.938 ± 0.059	8.734GlyVal: 8.734 ± 0.068	1.757GlyTrp: 1.757 ± 0.028	2.3GlyTyr: 2.3 ± 0.029	0.0GlyXaa: 0.0 ± 0.0
His	2.78HisAla: 2.78 ± 0.036	0.2HisCys: 0.2 ± 0.008	1.579HisAsp: 1.579 ± 0.025	1.299HisGlu: 1.299 ± 0.025	0.625HisPhe: 0.625 ± 0.015	2.333HisGly: 2.333 ± 0.028	0.746HisHis: 0.746 ± 0.017	0.523HisIle: 0.523 ± 0.015	0.317HisLys: 0.317 ± 0.011	2.526HisLeu: 2.526 ± 0.036	0.265HisMet: 0.265 ± 0.009	0.422HisAsn: 0.422 ± 0.013	1.716HisPro: 1.716 ± 0.028	0.652HisGln: 0.652 ± 0.015	2.284HisArg: 2.284 ± 0.033	0.948HisSer: 0.948 ± 0.019	1.18HisThr: 1.18 ± 0.023	2.16HisVal: 2.16 ± 0.029	0.362HisTrp: 0.362 ± 0.012	0.506HisTyr: 0.506 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.684IleAla: 4.684 ± 0.044	0.216IleCys: 0.216 ± 0.01	2.099IleAsp: 2.099 ± 0.033	1.746IleGlu: 1.746 ± 0.032	0.628IlePhe: 0.628 ± 0.018	3.514IleGly: 3.514 ± 0.042	0.518IleHis: 0.518 ± 0.014	0.785IleIle: 0.785 ± 0.022	0.645IleLys: 0.645 ± 0.017	1.904IleLeu: 1.904 ± 0.031	0.384IleMet: 0.384 ± 0.013	0.654IleAsn: 0.654 ± 0.017	1.622IlePro: 1.622 ± 0.027	0.61IleGln: 0.61 ± 0.016	2.045IleArg: 2.045 ± 0.029	1.64IleSer: 1.64 ± 0.028	2.413IleThr: 2.413 ± 0.031	2.524IleVal: 2.524 ± 0.038	0.333IleTrp: 0.333 ± 0.011	0.488IleTyr: 0.488 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.62LysAla: 2.62 ± 0.04	0.114LysCys: 0.114 ± 0.008	0.994LysAsp: 0.994 ± 0.024	0.845LysGlu: 0.845 ± 0.022	0.497LysPhe: 0.497 ± 0.017	1.392LysGly: 1.392 ± 0.027	0.448LysHis: 0.448 ± 0.014	0.728LysIle: 0.728 ± 0.018	0.526LysLys: 0.526 ± 0.022	2.071LysLeu: 2.071 ± 0.035	0.314LysMet: 0.314 ± 0.012	0.374LysAsn: 0.374 ± 0.014	1.359LysPro: 1.359 ± 0.025	0.651LysGln: 0.651 ± 0.019	1.435LysArg: 1.435 ± 0.031	1.031LysSer: 1.031 ± 0.017	1.155LysThr: 1.155 ± 0.023	1.97LysVal: 1.97 ± 0.034	0.307LysTrp: 0.307 ± 0.012	0.401LysTyr: 0.401 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.515LeuAla: 15.515 ± 0.109	0.789LeuCys: 0.789 ± 0.021	7.395LeuAsp: 7.395 ± 0.056	4.537LeuGlu: 4.537 ± 0.043	2.678LeuPhe: 2.678 ± 0.037	9.305LeuGly: 9.305 ± 0.062	2.377LeuHis: 2.377 ± 0.029	2.978LeuIle: 2.978 ± 0.038	1.815LeuLys: 1.815 ± 0.03	10.994LeuLeu: 10.994 ± 0.099	1.415LeuMet: 1.415 ± 0.023	1.763LeuAsn: 1.763 ± 0.025	6.472LeuPro: 6.472 ± 0.059	1.909LeuGln: 1.909 ± 0.028	9.247LeuArg: 9.247 ± 0.083	5.296LeuSer: 5.296 ± 0.048	6.486LeuThr: 6.486 ± 0.055	10.771LeuVal: 10.771 ± 0.083	1.352LeuTrp: 1.352 ± 0.024	1.705LeuTyr: 1.705 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.047MetAla: 2.047 ± 0.027	0.111MetCys: 0.111 ± 0.006	0.78MetAsp: 0.78 ± 0.021	0.565MetGlu: 0.565 ± 0.015	0.431MetPhe: 0.431 ± 0.012	1.155MetGly: 1.155 ± 0.023	0.313MetHis: 0.313 ± 0.01	0.591MetIle: 0.591 ± 0.016	0.339MetLys: 0.339 ± 0.011	1.603MetLeu: 1.603 ± 0.025	0.247MetMet: 0.247 ± 0.012	0.344MetAsn: 0.344 ± 0.013	0.974MetPro: 0.974 ± 0.02	0.357MetGln: 0.357 ± 0.012	1.386MetArg: 1.386 ± 0.023	1.169MetSer: 1.169 ± 0.022	1.414MetThr: 1.414 ± 0.023	1.38MetVal: 1.38 ± 0.025	0.189MetTrp: 0.189 ± 0.008	0.245MetTyr: 0.245 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.384AsnAla: 2.384 ± 0.036	0.177AsnCys: 0.177 ± 0.008	1.036AsnAsp: 1.036 ± 0.02	0.816AsnGlu: 0.816 ± 0.019	0.507AsnPhe: 0.507 ± 0.014	2.086AsnGly: 2.086 ± 0.041	0.448AsnHis: 0.448 ± 0.014	0.574AsnIle: 0.574 ± 0.016	0.344AsnLys: 0.344 ± 0.013	1.833AsnLeu: 1.833 ± 0.029	0.256AsnMet: 0.256 ± 0.009	0.488AsnAsn: 0.488 ± 0.019	1.55AsnPro: 1.55 ± 0.029	0.6AsnGln: 0.6 ± 0.019	1.435AsnArg: 1.435 ± 0.024	0.918AsnSer: 0.918 ± 0.023	1.237AsnThr: 1.237 ± 0.027	1.519AsnVal: 1.519 ± 0.027	0.32AsnTrp: 0.32 ± 0.012	0.469AsnTyr: 0.469 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	7.597ProAla: 7.597 ± 0.068	0.347ProCys: 0.347 ± 0.011	4.873ProAsp: 4.873 ± 0.049	3.985ProGlu: 3.985 ± 0.04	1.652ProPhe: 1.652 ± 0.025	6.079ProGly: 6.079 ± 0.056	1.341ProHis: 1.341 ± 0.023	1.487ProIle: 1.487 ± 0.026	1.148ProLys: 1.148 ± 0.021	5.164ProLeu: 5.164 ± 0.053	0.956ProMet: 0.956 ± 0.02	1.182ProAsn: 1.182 ± 0.024	3.803ProPro: 3.803 ± 0.068	1.436ProGln: 1.436 ± 0.027	3.826ProArg: 3.826 ± 0.04	3.04ProSer: 3.04 ± 0.039	3.688ProThr: 3.688 ± 0.045	5.896ProVal: 5.896 ± 0.059	0.956ProTrp: 0.956 ± 0.02	1.059ProTyr: 1.059 ± 0.017	0.0ProXaa: 0.0 ± 0.0
Gln	3.685GlnAla: 3.685 ± 0.047	0.16GlnCys: 0.16 ± 0.008	1.239GlnAsp: 1.239 ± 0.021	1.221GlnGlu: 1.221 ± 0.023	0.732GlnPhe: 0.732 ± 0.018	1.899GlnGly: 1.899 ± 0.032	0.619GlnHis: 0.619 ± 0.014	0.796GlnIle: 0.796 ± 0.018	0.478GlnLys: 0.478 ± 0.015	2.963GlnLeu: 2.963 ± 0.036	0.364GlnMet: 0.364 ± 0.013	0.483GlnAsn: 0.483 ± 0.015	1.78GlnPro: 1.78 ± 0.027	1.088GlnGln: 1.088 ± 0.024	2.554GlnArg: 2.554 ± 0.037	1.145GlnSer: 1.145 ± 0.02	1.189GlnThr: 1.189 ± 0.022	2.891GlnVal: 2.891 ± 0.033	0.505GlnTrp: 0.505 ± 0.014	0.545GlnTyr: 0.545 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	9.979ArgAla: 9.979 ± 0.085	0.606ArgCys: 0.606 ± 0.018	4.647ArgAsp: 4.647 ± 0.048	4.736ArgGlu: 4.736 ± 0.047	2.602ArgPhe: 2.602 ± 0.034	5.807ArgGly: 5.807 ± 0.054	2.176ArgHis: 2.176 ± 0.034	2.942ArgIle: 2.942 ± 0.035	1.665ArgLys: 1.665 ± 0.03	9.006ArgLeu: 9.006 ± 0.079	1.771ArgMet: 1.771 ± 0.029	1.483ArgAsn: 1.483 ± 0.028	4.755ArgPro: 4.755 ± 0.046	2.339ArgGln: 2.339 ± 0.034	7.782ArgArg: 7.782 ± 0.074	3.953ArgSer: 3.953 ± 0.044	4.97ArgThr: 4.97 ± 0.05	7.068ArgVal: 7.068 ± 0.054	1.562ArgTrp: 1.562 ± 0.025	1.863ArgTyr: 1.863 ± 0.027	0.0ArgXaa: 0.0 ± 0.0
Ser	6.505SerAla: 6.505 ± 0.052	0.391SerCys: 0.391 ± 0.014	2.631SerAsp: 2.631 ± 0.032	2.137SerGlu: 2.137 ± 0.027	1.582SerPhe: 1.582 ± 0.022	5.612SerGly: 5.612 ± 0.051	0.905SerHis: 0.905 ± 0.016	1.437SerIle: 1.437 ± 0.024	0.876SerLys: 0.876 ± 0.019	4.672SerLeu: 4.672 ± 0.045	0.971SerMet: 0.971 ± 0.018	0.911SerAsn: 0.911 ± 0.021	3.053SerPro: 3.053 ± 0.034	1.106SerGln: 1.106 ± 0.023	3.639SerArg: 3.639 ± 0.038	2.759SerSer: 2.759 ± 0.042	3.436SerThr: 3.436 ± 0.041	4.5SerVal: 4.5 ± 0.047	0.975SerTrp: 0.975 ± 0.02	1.086SerTyr: 1.086 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	8.58ThrAla: 8.58 ± 0.065	0.465ThrCys: 0.465 ± 0.014	3.567ThrAsp: 3.567 ± 0.039	3.021ThrGlu: 3.021 ± 0.035	1.723ThrPhe: 1.723 ± 0.028	6.553ThrGly: 6.553 ± 0.059	1.134ThrHis: 1.134 ± 0.022	1.867ThrIle: 1.867 ± 0.031	1.155ThrLys: 1.155 ± 0.025	5.322ThrLeu: 5.322 ± 0.054	0.908ThrMet: 0.908 ± 0.02	1.151ThrAsn: 1.151 ± 0.025	4.073ThrPro: 4.073 ± 0.05	1.223ThrGln: 1.223 ± 0.023	4.148ThrArg: 4.148 ± 0.041	3.565ThrSer: 3.565 ± 0.043	4.954ThrThr: 4.954 ± 0.088	6.048ThrVal: 6.048 ± 0.058	1.05ThrTrp: 1.05 ± 0.023	1.268ThrTyr: 1.268 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	12.391ValAla: 12.391 ± 0.086	0.696ValCys: 0.696 ± 0.018	6.69ValAsp: 6.69 ± 0.044	5.858ValGlu: 5.858 ± 0.053	2.701ValPhe: 2.701 ± 0.034	7.616ValGly: 7.616 ± 0.068	2.179ValHis: 2.179 ± 0.027	2.891ValIle: 2.891 ± 0.037	1.716ValLys: 1.716 ± 0.032	11.32ValLeu: 11.32 ± 0.087	1.269ValMet: 1.269 ± 0.024	1.909ValAsn: 1.909 ± 0.032	5.795ValPro: 5.795 ± 0.055	2.16ValGln: 2.16 ± 0.034	7.972ValArg: 7.972 ± 0.068	4.591ValSer: 4.591 ± 0.045	6.188ValThr: 6.188 ± 0.053	10.961ValVal: 10.961 ± 0.084	1.218ValTrp: 1.218 ± 0.023	1.538ValTyr: 1.538 ± 0.025	0.0ValXaa: 0.0 ± 0.0
Trp	1.696TrpAla: 1.696 ± 0.027	0.153TrpCys: 0.153 ± 0.008	0.872TrpAsp: 0.872 ± 0.019	0.71TrpGlu: 0.71 ± 0.017	0.557TrpPhe: 0.557 ± 0.016	1.055TrpGly: 1.055 ± 0.02	0.432TrpHis: 0.432 ± 0.013	0.472TrpIle: 0.472 ± 0.014	0.271TrpLys: 0.271 ± 0.012	1.951TrpLeu: 1.951 ± 0.03	0.28TrpMet: 0.28 ± 0.011	0.431TrpAsn: 0.431 ± 0.014	0.85TrpPro: 0.85 ± 0.019	0.637TrpGln: 0.637 ± 0.016	1.546TrpArg: 1.546 ± 0.026	1.053TrpSer: 1.053 ± 0.023	1.103TrpThr: 1.103 ± 0.023	1.219TrpVal: 1.219 ± 0.024	0.388TrpTrp: 0.388 ± 0.014	0.305TrpTyr: 0.305 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.37TyrAla: 2.37 ± 0.033	0.167TyrCys: 0.167 ± 0.008	1.332TyrAsp: 1.332 ± 0.031	1.025TyrGlu: 1.025 ± 0.019	0.651TyrPhe: 0.651 ± 0.017	1.883TyrGly: 1.883 ± 0.031	0.425TyrHis: 0.425 ± 0.013	0.386TyrIle: 0.386 ± 0.012	0.314TyrLys: 0.314 ± 0.012	2.246TyrLeu: 2.246 ± 0.026	0.192TyrMet: 0.192 ± 0.009	0.439TyrAsn: 0.439 ± 0.014	1.094TyrPro: 1.094 ± 0.023	0.639TyrGln: 0.639 ± 0.015	1.951TyrArg: 1.951 ± 0.031	0.966TyrSer: 0.966 ± 0.021	1.136TyrThr: 1.136 ± 0.025	1.656TyrVal: 1.656 ± 0.027	0.337TyrTrp: 0.337 ± 0.011	0.5TyrTyr: 0.5 ± 0.012	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7942 proteins (2649883 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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