Xinzhou nematode virus 7

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 9.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH95|A0A1L3KH95_9VIRU RNA-directed RNA polymerase OS=Xinzhou nematode virus 7 OX=1923775 PE=4 SV=1
MM1 pKa = 7.45GLGASAMVAMHH12 pKa = 6.61SSVDD16 pKa = 3.75MQTSMPTAGVVIGGVISRR34 pKa = 11.84GPFKK38 pKa = 10.39PLRR41 pKa = 11.84VGATIRR47 pKa = 11.84YY48 pKa = 8.08GYY50 pKa = 9.58YY51 pKa = 10.2GRR53 pKa = 11.84RR54 pKa = 11.84PQSTRR59 pKa = 11.84IQSLFPDD66 pKa = 3.95VEE68 pKa = 4.23GLLPLEE74 pKa = 4.16TRR76 pKa = 11.84TVNVHH81 pKa = 5.32LVCWEE86 pKa = 3.93NEE88 pKa = 4.14IVCLRR93 pKa = 11.84NRR95 pKa = 11.84VLMQTPQPRR104 pKa = 11.84PHH106 pKa = 7.77SMGRR110 pKa = 11.84LQLHH114 pKa = 5.58GHH116 pKa = 6.4ALCSIVLSFIGRR128 pKa = 11.84LTPWSLSKK136 pKa = 9.52TLRR139 pKa = 11.84HH140 pKa = 4.76MVVRR144 pKa = 11.84HH145 pKa = 5.42GAALYY150 pKa = 10.39VPAAEE155 pKa = 4.76SLRR158 pKa = 11.84QEE160 pKa = 4.11PLGSIDD166 pKa = 3.6ARR168 pKa = 11.84VSMFLKK174 pKa = 10.05VEE176 pKa = 4.14KK177 pKa = 10.9VFAEE181 pKa = 4.55DD182 pKa = 3.57PAEE185 pKa = 4.23KK186 pKa = 10.23APRR189 pKa = 11.84AIQYY193 pKa = 8.77RR194 pKa = 11.84GPRR197 pKa = 11.84FNLVLGRR204 pKa = 11.84FILAYY209 pKa = 10.15EE210 pKa = 3.96EE211 pKa = 3.83AFYY214 pKa = 10.89RR215 pKa = 11.84AFNHH219 pKa = 5.42VNASRR224 pKa = 11.84CHH226 pKa = 5.47TSKK229 pKa = 11.16GLTPDD234 pKa = 3.17ARR236 pKa = 11.84AALLWEE242 pKa = 4.68LWSKK246 pKa = 10.68NPRR249 pKa = 11.84PAALNVDD256 pKa = 3.91ASRR259 pKa = 11.84FDD261 pKa = 3.23AHH263 pKa = 5.69VTVEE267 pKa = 4.0MLLLEE272 pKa = 4.14SLYY275 pKa = 11.03YY276 pKa = 10.41EE277 pKa = 4.43SSFPNCRR284 pKa = 11.84LLRR287 pKa = 11.84WLLRR291 pKa = 11.84CQRR294 pKa = 11.84RR295 pKa = 11.84NRR297 pKa = 11.84GCGKK301 pKa = 10.52FGTTYY306 pKa = 8.58TLKK309 pKa = 10.15GGRR312 pKa = 11.84MSGDD316 pKa = 3.13VNTALGNTLIQMTVLSFLAGRR337 pKa = 11.84SFDD340 pKa = 4.12LVVEE344 pKa = 4.49GDD346 pKa = 3.68DD347 pKa = 3.82AVIFGDD353 pKa = 3.34VDD355 pKa = 4.59DD356 pKa = 4.17ITRR359 pKa = 11.84LEE361 pKa = 3.89AVITSKK367 pKa = 11.23ALAVGFQLKK376 pKa = 9.88VSKK379 pKa = 10.6ALYY382 pKa = 10.24LEE384 pKa = 3.86QLEE387 pKa = 4.58YY388 pKa = 11.09CSTRR392 pKa = 11.84VIRR395 pKa = 11.84SQSGDD400 pKa = 2.95VRR402 pKa = 11.84AIRR405 pKa = 11.84EE406 pKa = 4.32WPRR409 pKa = 11.84PLAMDD414 pKa = 4.66RR415 pKa = 11.84YY416 pKa = 7.27TVKK419 pKa = 10.28IVSGQRR425 pKa = 11.84ATNRR429 pKa = 11.84KK430 pKa = 7.88ARR432 pKa = 11.84TMAVCFMSLYY442 pKa = 10.55SGLPVYY448 pKa = 9.63EE449 pKa = 3.88EE450 pKa = 3.76WARR453 pKa = 11.84YY454 pKa = 8.24LLSWSDD460 pKa = 3.2PSFGVDD466 pKa = 3.06EE467 pKa = 4.57SQDD470 pKa = 3.09RR471 pKa = 11.84RR472 pKa = 11.84LWFIAAEE479 pKa = 4.23ACVGTGDD486 pKa = 4.31QVPADD491 pKa = 3.89GLRR494 pKa = 11.84SSFYY498 pKa = 9.36MATTIEE504 pKa = 4.28PSEE507 pKa = 3.99QLRR510 pKa = 11.84VEE512 pKa = 4.11GLLRR516 pKa = 11.84DD517 pKa = 3.51QRR519 pKa = 11.84GPHH522 pKa = 6.47PAYY525 pKa = 9.82ILGGEE530 pKa = 4.04LRR532 pKa = 11.84ALRR535 pKa = 11.84RR536 pKa = 11.84AAAQLQNSAPSSS548 pKa = 3.56

Molecular weight: 61.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH95|A0A1L3KH95_9VIRU RNA-directed RNA polymerase OS=Xinzhou nematode virus 7 OX=1923775 PE=4 SV=1
MM1 pKa = 7.87DD2 pKa = 4.5NEE4 pKa = 4.19MLRR7 pKa = 11.84GGRR10 pKa = 11.84GPPINQPRR18 pKa = 11.84APPPDD23 pKa = 3.27RR24 pKa = 11.84RR25 pKa = 11.84GGAGRR30 pKa = 11.84RR31 pKa = 11.84TRR33 pKa = 11.84NQVGADD39 pKa = 3.15QLGFRR44 pKa = 11.84RR45 pKa = 11.84GPRR48 pKa = 11.84TQLRR52 pKa = 11.84GPSRR56 pKa = 11.84VRR58 pKa = 11.84SSRR61 pKa = 11.84ASVARR66 pKa = 11.84GGRR69 pKa = 11.84GNFPPPPPRR78 pKa = 11.84GPAQFEE84 pKa = 4.14ARR86 pKa = 11.84GMPHH90 pKa = 6.45HH91 pKa = 7.11RR92 pKa = 11.84GSQGLPRR99 pKa = 11.84PLPAPGQQQAAPVDD113 pKa = 4.03HH114 pKa = 7.07FPPLVPRR121 pKa = 11.84EE122 pKa = 4.39GEE124 pKa = 4.04PGPQPVLPCVVQWPCVPAPPGPAGPQAQPPAPPGAVNCGAPQRR167 pKa = 11.84HH168 pKa = 5.09RR169 pKa = 11.84VAPIRR174 pKa = 11.84ALSEE178 pKa = 3.91GGEE181 pKa = 4.08PFEE184 pKa = 4.88PEE186 pKa = 3.82VGPTLRR192 pKa = 11.84DD193 pKa = 2.89RR194 pKa = 11.84CRR196 pKa = 11.84EE197 pKa = 3.89YY198 pKa = 11.03LSRR201 pKa = 11.84RR202 pKa = 11.84LVWLARR208 pKa = 11.84TPEE211 pKa = 3.49NRR213 pKa = 11.84AAVVRR218 pKa = 11.84AARR221 pKa = 11.84TFVSSQDD228 pKa = 3.28PDD230 pKa = 4.05CLVRR234 pKa = 11.84APWLSALIDD243 pKa = 4.0EE244 pKa = 4.97LWFDD248 pKa = 4.22LRR250 pKa = 11.84VEE252 pKa = 4.24LEE254 pKa = 4.17LGRR257 pKa = 11.84VRR259 pKa = 11.84PWSVALHH266 pKa = 5.73EE267 pKa = 5.0ARR269 pKa = 11.84HH270 pKa = 4.5AHH272 pKa = 4.67YY273 pKa = 11.11VEE275 pKa = 4.69GRR277 pKa = 11.84AHH279 pKa = 6.89EE280 pKa = 5.35LFTPWRR286 pKa = 11.84LLNYY290 pKa = 7.44EE291 pKa = 3.98QSLGLDD297 pKa = 3.08QLSAYY302 pKa = 7.13WRR304 pKa = 11.84PIALCAGGAGLFCGAVAIGCWLGGAHH330 pKa = 6.68RR331 pKa = 11.84RR332 pKa = 11.84LPFEE336 pKa = 4.03VVLRR340 pKa = 11.84WGG342 pKa = 3.34

Molecular weight: 37.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

890

342

548

445.0

49.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.663 ± 0.538

2.022 ± 0.197

3.596 ± 0.418

5.169 ± 0.059

3.371 ± 0.278

8.764 ± 1.462

2.247 ± 0.239

2.809 ± 0.839

1.573 ± 0.98

10.449 ± 0.863

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.135 ± 0.783

2.36 ± 0.195

8.652 ± 3.535

4.157 ± 0.507

11.011 ± 1.337

6.292 ± 1.551

3.708 ± 1.217

7.528 ± 0.318

2.022 ± 0.379

2.472 ± 0.811
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski