Synechococcus T7-like phage S-TIP37

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Igirivirus; Synechococcus virus STIP37

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345AY85|A0A345AY85_9CAUD Uncharacterized protein OS=Synechococcus T7-like phage S-TIP37 OX=1332145 GN=STIP37_3 PE=4 SV=1
MM1 pKa = 7.05TVATVRR7 pKa = 11.84TFDD10 pKa = 3.49ILGHH14 pKa = 5.88EE15 pKa = 4.44FDD17 pKa = 4.34MDD19 pKa = 4.12EE20 pKa = 4.95LNDD23 pKa = 3.65IATHH27 pKa = 4.98GMEE30 pKa = 4.57GGVSGFIYY38 pKa = 10.42SSEE41 pKa = 3.95LFDD44 pKa = 4.32IYY46 pKa = 10.88EE47 pKa = 4.1EE48 pKa = 4.84HH49 pKa = 7.64EE50 pKa = 4.19DD51 pKa = 3.86TIMSYY56 pKa = 11.05LDD58 pKa = 3.01EE59 pKa = 4.2WAFDD63 pKa = 3.56VGEE66 pKa = 4.42DD67 pKa = 3.0NGFRR71 pKa = 11.84MVLNSMDD78 pKa = 3.4RR79 pKa = 11.84RR80 pKa = 11.84GIEE83 pKa = 4.02YY84 pKa = 7.58DD85 pKa = 3.1TLQIFKK91 pKa = 9.99EE92 pKa = 3.96QAVWMFVEE100 pKa = 4.84LFAVQLLQRR109 pKa = 11.84NGHH112 pKa = 6.19PDD114 pKa = 2.86WVV116 pKa = 3.63

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345AYD7|A0A345AYD7_9CAUD HNH endonuclease OS=Synechococcus T7-like phage S-TIP37 OX=1332145 GN=STIP37_50 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 5.67LGEE5 pKa = 4.57PPVLPVLRR13 pKa = 11.84LPEE16 pKa = 4.62APQLPRR22 pKa = 11.84PVLEE26 pKa = 4.33VPRR29 pKa = 11.84AEE31 pKa = 4.01IPSYY35 pKa = 10.66KK36 pKa = 9.93PLVVPPSDD44 pKa = 3.87LRR46 pKa = 11.84PPPGVKK52 pKa = 9.0GTTEE56 pKa = 3.88SDD58 pKa = 3.48KK59 pKa = 11.39EE60 pKa = 4.17NQNLSRR66 pKa = 11.84PSCRR70 pKa = 11.84IYY72 pKa = 10.11RR73 pKa = 11.84QIRR76 pKa = 11.84GRR78 pKa = 11.84STSHH82 pKa = 6.36LRR84 pKa = 11.84TRR86 pKa = 11.84QCLFRR91 pKa = 11.84RR92 pKa = 11.84TKK94 pKa = 10.04YY95 pKa = 10.54LSRR98 pKa = 11.84LALLPPSPLQPPSQQQQSSNGLL120 pKa = 3.44

Molecular weight: 13.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13760

38

1207

245.7

27.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.135 ± 0.69

0.981 ± 0.163

6.526 ± 0.263

6.119 ± 0.428

3.735 ± 0.257

7.049 ± 0.391

1.991 ± 0.237

5.211 ± 0.22

5.523 ± 0.402

7.9 ± 0.432

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.296

5.167 ± 0.475

4.07 ± 0.327

4.092 ± 0.368

4.978 ± 0.408

6.759 ± 0.459

7.282 ± 0.812

6.381 ± 0.422

1.192 ± 0.143

3.27 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski