Cyanophage S-RIM4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MNX7|A0A482MNX7_9CAUD Tail fiber protein OS=Cyanophage S-RIM4 OX=2530169 GN=RW110999_045 PE=4 SV=1
MM1 pKa = 7.99ADD3 pKa = 3.41QGILAQSKK11 pKa = 8.97PAAATDD17 pKa = 3.72TVLYY21 pKa = 9.28RR22 pKa = 11.84APVDD26 pKa = 3.68QSASTVLNIANDD38 pKa = 4.41GTGSAYY44 pKa = 10.46SVAVKK49 pKa = 10.38DD50 pKa = 3.48YY51 pKa = 10.75DD52 pKa = 4.17QRR54 pKa = 11.84LTLDD58 pKa = 3.06ASTYY62 pKa = 10.32LLHH65 pKa = 7.08PGDD68 pKa = 4.96IISSYY73 pKa = 11.61SLVFDD78 pKa = 4.22VNMTASSGFTPGQLITTDD96 pKa = 3.8DD97 pKa = 4.03GEE99 pKa = 4.08KK100 pKa = 10.38SFRR103 pKa = 11.84FEE105 pKa = 3.92SFRR108 pKa = 11.84VPEE111 pKa = 3.9FTEE114 pKa = 3.71IFVKK118 pKa = 10.35IEE120 pKa = 4.11SIRR123 pKa = 11.84AITVEE128 pKa = 4.39SVSGTFTVGSTVSTGVAPDD147 pKa = 3.49DD148 pKa = 3.85TTAVIYY154 pKa = 10.28GVLDD158 pKa = 3.48QSTSAILYY166 pKa = 7.83VGPSTINGSGVEE178 pKa = 4.12FADD181 pKa = 4.08GDD183 pKa = 4.15SLAITGASGTISAGGIAAAADD204 pKa = 3.52EE205 pKa = 4.97FVFSITTAGGTYY217 pKa = 10.59NSFLTEE223 pKa = 4.05TTSLFNDD230 pKa = 3.17RR231 pKa = 11.84TYY233 pKa = 11.3RR234 pKa = 11.84FDD236 pKa = 3.71VSDD239 pKa = 3.77SSMTGSDD246 pKa = 3.34FALSFTANGIYY257 pKa = 10.66GPDD260 pKa = 3.58GAIGGVSPDD269 pKa = 4.32DD270 pKa = 3.28GTEE273 pKa = 3.95YY274 pKa = 11.3VFGQTVNGTPGSAGAYY290 pKa = 7.96IQYY293 pKa = 10.43DD294 pKa = 4.09FNAGATTNDD303 pKa = 3.61PVTGVPYY310 pKa = 10.47FGGSILYY317 pKa = 10.03FYY319 pKa = 10.95DD320 pKa = 3.46NGDD323 pKa = 3.57AAFGGSDD330 pKa = 3.33RR331 pKa = 11.84QFLINSSTEE340 pKa = 3.68FNEE343 pKa = 3.7FFIYY347 pKa = 10.44DD348 pKa = 3.83LEE350 pKa = 5.96GSLVASSDD358 pKa = 3.43TFEE361 pKa = 4.44VQGVTYY367 pKa = 9.53TLQTINAGPYY377 pKa = 8.8GYY379 pKa = 10.29VRR381 pKa = 11.84RR382 pKa = 11.84YY383 pKa = 10.43SGTTLDD389 pKa = 3.99VIKK392 pKa = 10.99GPGSQDD398 pKa = 3.03FAGTDD403 pKa = 3.69TIFDD407 pKa = 3.76NPKK410 pKa = 10.75DD411 pKa = 3.69GDD413 pKa = 3.7ATRR416 pKa = 11.84SLMTVSSVDD425 pKa = 3.43VASAAVTAEE434 pKa = 3.83NYY436 pKa = 8.32LTVGATNSANNVDD449 pKa = 4.86KK450 pKa = 10.4ITSLVVGPGEE460 pKa = 4.07VVVVNSTTQNNVFSLVGFEE479 pKa = 4.6DD480 pKa = 3.68QATSFPTRR488 pKa = 11.84VFDD491 pKa = 4.94FSS493 pKa = 3.53

Molecular weight: 51.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MN93|A0A482MN93_9CAUD Baseplate wedge protein OS=Cyanophage S-RIM4 OX=2530169 GN=RW110999_098 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.38ALTKK6 pKa = 10.24RR7 pKa = 11.84RR8 pKa = 11.84SANYY12 pKa = 8.56FANQVKK18 pKa = 10.15VLLLILIGVLIYY30 pKa = 10.12QSKK33 pKa = 10.14DD34 pKa = 2.93ARR36 pKa = 11.84FFISDD41 pKa = 3.81RR42 pKa = 11.84LNDD45 pKa = 3.9ASEE48 pKa = 4.55MIRR51 pKa = 11.84PAPQFEE57 pKa = 3.85IRR59 pKa = 11.84YY60 pKa = 9.03

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

228

0

228

55635

37

6026

244.0

27.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.107 ± 0.212

0.944 ± 0.102

6.766 ± 0.126

6.167 ± 0.255

4.404 ± 0.114

7.531 ± 0.315

1.447 ± 0.137

6.311 ± 0.193

5.852 ± 0.429

7.299 ± 0.133

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.148 ± 0.224

5.782 ± 0.203

4.015 ± 0.134

3.726 ± 0.098

4.166 ± 0.151

6.73 ± 0.198

7.4 ± 0.352

6.751 ± 0.202

1.165 ± 0.071

4.289 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski