Capybara microvirus Cap3_SP_347
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5P7|A0A4P8W5P7_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_347 OX=2585433 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.13 KK3 pKa = 9.81 FQKK6 pKa = 10.29 FPPDD10 pKa = 3.32 GNVIIPSINSFEE22 pKa = 4.29 ASFNADD28 pKa = 2.58 GSVTFVPYY36 pKa = 10.21 SAPSPFIASDD46 pKa = 3.3 VDD48 pKa = 3.85 IPSLLIAGITPQSSHH63 pKa = 6.01 EE64 pKa = 4.15 MEE66 pKa = 4.41 LTPVDD71 pKa = 4.29 SLSSLQQFEE80 pKa = 5.17 HH81 pKa = 6.68 EE82 pKa = 4.25 FDD84 pKa = 3.54 NFKK87 pKa = 11.09 SS88 pKa = 3.53
Molecular weight: 9.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.447
IPC2_protein 4.342
IPC_protein 4.202
Toseland 4.024
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.101
Rodwell 4.037
Grimsley 3.935
Solomon 4.164
Lehninger 4.113
Nozaki 4.304
DTASelect 4.482
Thurlkill 4.075
EMBOSS 4.113
Sillero 4.317
Patrickios 3.897
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.228
Protein with the highest isoelectric point:
>tr|A0A4P8W4W7|A0A4P8W4W7_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_347 OX=2585433 PE=4 SV=1
MM1 pKa = 7.61 ANYY4 pKa = 9.37 FKK6 pKa = 11.17 LFLIYY11 pKa = 10.65 LIYY14 pKa = 10.16 FYY16 pKa = 10.59 LYY18 pKa = 10.87 SLFIYY23 pKa = 10.11 SPHH26 pKa = 7.01 RR27 pKa = 11.84 AGGCLGGSLILDD39 pKa = 4.4 FSHH42 pKa = 7.49 LSIIPILFFYY52 pKa = 7.86 YY53 pKa = 9.2 FCYY56 pKa = 10.18 FIFIFFYY63 pKa = 10.42 FCIVKK68 pKa = 10.09 II69 pKa = 4.0
Molecular weight: 8.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.882
IPC2_protein 7.6
IPC_protein 7.775
Toseland 7.263
ProMoST 8.024
Dawson 8.214
Bjellqvist 8.478
Wikipedia 8.17
Rodwell 8.2
Grimsley 7.205
Solomon 8.434
Lehninger 8.434
Nozaki 8.521
DTASelect 8.287
Thurlkill 8.302
EMBOSS 8.434
Sillero 8.609
Patrickios 0.006
IPC_peptide 8.419
IPC2_peptide 7.98
IPC2.peptide.svr19 8.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1724
69
610
287.3
33.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.162 ± 1.798
1.856 ± 0.512
7.077 ± 0.814
4.118 ± 0.719
7.831 ± 1.741
4.756 ± 0.637
3.248 ± 1.024
5.51 ± 0.668
6.032 ± 0.715
9.687 ± 0.785
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.03 ± 0.159
6.265 ± 0.688
3.712 ± 0.67
3.132 ± 0.692
3.48 ± 0.464
9.397 ± 1.167
4.06 ± 0.31
5.394 ± 0.704
0.58 ± 0.171
6.671 ± 1.446
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here