Modestobacter muralis
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4084 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6P0H6E2|A0A6P0H6E2_9ACTN Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase OS=Modestobacter muralis OX=1608614 GN=lgt PE=3 SV=1
MM1 pKa = 7.51 SSANRR6 pKa = 11.84 TGLTALALAAGLLLAGCGGDD26 pKa = 4.39 ADD28 pKa = 5.3 ADD30 pKa = 3.82 TAAASGGHH38 pKa = 5.45 DD39 pKa = 3.73 HH40 pKa = 7.17 GGAAAPATVAGGEE53 pKa = 4.0 DD54 pKa = 3.45 SPYY57 pKa = 10.93 SGLYY61 pKa = 9.82 LQDD64 pKa = 4.64 PYY66 pKa = 11.44 QKK68 pKa = 10.74 PEE70 pKa = 3.67 FTLTDD75 pKa = 3.51 GAGAPFDD82 pKa = 3.8 FAEE85 pKa = 4.43 RR86 pKa = 11.84 TAAGPTLLFFGYY98 pKa = 7.92 TNCPDD103 pKa = 3.37 VCPTTMADD111 pKa = 2.95 VALALRR117 pKa = 11.84 TTDD120 pKa = 3.72 PAVVAATNVVFVTTDD135 pKa = 3.51 PARR138 pKa = 11.84 DD139 pKa = 3.57 TPEE142 pKa = 3.88 VLDD145 pKa = 4.45 EE146 pKa = 3.91 YY147 pKa = 11.05 LGRR150 pKa = 11.84 FDD152 pKa = 5.93 ADD154 pKa = 3.35 LGTSFVGLTGDD165 pKa = 3.37 QAQIEE170 pKa = 4.46 QAQLAAGVPLAEE182 pKa = 5.5 DD183 pKa = 3.19 NGEE186 pKa = 4.13 THH188 pKa = 7.02 SAMLLLYY195 pKa = 8.97 GTDD198 pKa = 3.6 GEE200 pKa = 4.75 ADD202 pKa = 3.5 VAFNGGNTATDD213 pKa = 3.43 IAHH216 pKa = 7.15 DD217 pKa = 4.08 LAVVAAEE224 pKa = 3.98
Molecular weight: 22.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.656
IPC_protein 3.681
Toseland 3.452
ProMoST 3.834
Dawson 3.681
Bjellqvist 3.872
Wikipedia 3.643
Rodwell 3.503
Grimsley 3.363
Solomon 3.668
Lehninger 3.63
Nozaki 3.795
DTASelect 4.062
Thurlkill 3.516
EMBOSS 3.643
Sillero 3.795
Patrickios 0.731
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.75
Protein with the highest isoelectric point:
>tr|A0A6P0EQD6|A0A6P0EQD6_9ACTN Histidine kinase OS=Modestobacter muralis OX=1608614 GN=G3R41_03915 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.51 LLKK22 pKa = 8.15 KK23 pKa = 9.25 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.81 KK32 pKa = 9.63
Molecular weight: 4.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4084
0
4084
1277771
26
2326
312.9
33.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.608 ± 0.058
0.632 ± 0.011
6.198 ± 0.033
5.215 ± 0.036
2.523 ± 0.023
9.668 ± 0.041
2.009 ± 0.018
2.683 ± 0.031
1.339 ± 0.024
10.722 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.577 ± 0.017
1.447 ± 0.019
6.139 ± 0.038
2.891 ± 0.021
7.792 ± 0.046
5.019 ± 0.024
6.348 ± 0.029
10.014 ± 0.038
1.479 ± 0.018
1.696 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here