Sus scrofa polyomavirus 1
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A162GN61|A0A162GN61_9POLY Minor capsid protein VP2 OS=Sus scrofa polyomavirus 1 OX=1680894 GN=VP3 PE=3 SV=1
MM1 pKa = 7.62 GGFLSLILDD10 pKa = 4.53 LSTIAVDD17 pKa = 4.54 LSASTGIALEE27 pKa = 4.83 AILTGEE33 pKa = 4.08 ALAALEE39 pKa = 4.48 AEE41 pKa = 4.86 VTALMTLEE49 pKa = 4.3 GLSGVEE55 pKa = 4.72 ALAQLGFTAEE65 pKa = 3.9 QFANFSLVSSIVNQAVTYY83 pKa = 7.6 GTIFQTVSGASALISAGIRR102 pKa = 11.84 LGLEE106 pKa = 3.55 EE107 pKa = 5.27 RR108 pKa = 11.84 SIVDD112 pKa = 4.59 LNLHH116 pKa = 5.5 GQGLTINGLSKK127 pKa = 10.09 EE128 pKa = 4.89 TITHH132 pKa = 5.65 MASGFAFDD140 pKa = 4.15 PFHH143 pKa = 6.42 WSEE146 pKa = 4.4 SLLHH150 pKa = 6.24 SIGRR154 pKa = 11.84 GINRR158 pKa = 11.84 MTEE161 pKa = 3.89 LPVNNNLARR170 pKa = 11.84 LAEE173 pKa = 4.08 EE174 pKa = 4.95 GRR176 pKa = 11.84 WKK178 pKa = 10.42 LQRR181 pKa = 11.84 EE182 pKa = 4.5 GTDD185 pKa = 3.65 DD186 pKa = 3.98 GDD188 pKa = 3.93 SGEE191 pKa = 4.53 VIAMYY196 pKa = 8.7 TPPGGSHH203 pKa = 6.39 QRR205 pKa = 11.84 VCPDD209 pKa = 2.66 WLLPLVLGLSGSPRR223 pKa = 11.84 GII225 pKa = 3.59
Molecular weight: 23.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.668
IPC2_protein 4.635
IPC_protein 4.533
Toseland 4.406
ProMoST 4.635
Dawson 4.469
Bjellqvist 4.622
Wikipedia 4.329
Rodwell 4.38
Grimsley 4.317
Solomon 4.469
Lehninger 4.431
Nozaki 4.584
DTASelect 4.698
Thurlkill 4.406
EMBOSS 4.342
Sillero 4.647
Patrickios 3.948
IPC_peptide 4.482
IPC2_peptide 4.647
IPC2.peptide.svr19 4.577
Protein with the highest isoelectric point:
>tr|A0A162GNC4|A0A162GNC4_9POLY 93 T antigen OS=Sus scrofa polyomavirus 1 OX=1680894 GN=93 TAg PE=4 SV=1
MM1 pKa = 8.08 DD2 pKa = 4.21 IALTRR7 pKa = 11.84 EE8 pKa = 3.85 EE9 pKa = 4.06 RR10 pKa = 11.84 KK11 pKa = 9.89 EE12 pKa = 3.81 LSEE15 pKa = 4.47 LLDD18 pKa = 4.02 LAPHH22 pKa = 6.75 CFGNIPMMKK31 pKa = 8.88 FCYY34 pKa = 9.79 RR35 pKa = 11.84 KK36 pKa = 9.81 ACLRR40 pKa = 11.84 LHH42 pKa = 6.79 PDD44 pKa = 2.83 KK45 pKa = 11.56 GGDD48 pKa = 3.23 AGKK51 pKa = 8.45 MQRR54 pKa = 11.84 LNEE57 pKa = 4.09 LWQTFQQSIDD67 pKa = 3.53 CLRR70 pKa = 11.84 NGEE73 pKa = 4.15 NAGFYY78 pKa = 10.52 SFQLPNRR85 pKa = 11.84 RR86 pKa = 11.84 LCIWRR91 pKa = 11.84 FPVFPVLLKK100 pKa = 10.47 KK101 pKa = 10.56 IPGASRR107 pKa = 11.84 ARR109 pKa = 11.84 PLLLRR114 pKa = 11.84 DD115 pKa = 3.5 PGFVSRR121 pKa = 11.84 ISKK124 pKa = 8.91 IHH126 pKa = 7.06 RR127 pKa = 11.84 IQHH130 pKa = 4.94 HH131 pKa = 6.38 HH132 pKa = 6.79 CLLVSGLYY140 pKa = 10.43 LPSPWKK146 pKa = 9.85 EE147 pKa = 3.31 ICNAFRR153 pKa = 11.84 GRR155 pKa = 11.84 FF156 pKa = 3.41
Molecular weight: 18.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.181
IPC2_protein 9.048
IPC_protein 9.077
Toseland 9.897
ProMoST 9.56
Dawson 10.072
Bjellqvist 9.823
Wikipedia 10.218
Rodwell 10.438
Grimsley 10.116
Solomon 10.131
Lehninger 10.116
Nozaki 10.087
DTASelect 9.75
Thurlkill 9.97
EMBOSS 10.306
Sillero 10.058
Patrickios 10.116
IPC_peptide 10.131
IPC2_peptide 9.019
IPC2.peptide.svr19 7.823
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
2979
93
782
229.2
25.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.109 ± 0.419
2.685 ± 0.51
5.136 ± 0.33
5.942 ± 0.226
5.102 ± 0.514
6.613 ± 0.769
2.551 ± 0.383
4.465 ± 0.428
5.505 ± 0.655
11.715 ± 1.316
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.685 ± 0.468
3.39 ± 0.337
6.781 ± 1.036
4.7 ± 0.558
6.344 ± 0.65
8.493 ± 1.594
4.901 ± 0.573
3.424 ± 0.682
1.276 ± 0.24
2.182 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here