Pseudomonas phage Noxifer

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Noxifervirus; Pseudomonas virus Noxifer

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y0T0U3|A0A1Y0T0U3_9CAUD Uncharacterized protein OS=Pseudomonas phage Noxifer OX=2006684 GN=NOXIFER_244 PE=4 SV=1
MM1 pKa = 7.67LFVRR5 pKa = 11.84DD6 pKa = 3.81NLVNSIEE13 pKa = 4.09PDD15 pKa = 3.21QTYY18 pKa = 10.84SAAEE22 pKa = 4.04VINYY26 pKa = 8.91VDD28 pKa = 3.35QGIEE32 pKa = 4.03GVLKK36 pKa = 10.8VVEE39 pKa = 4.38QQCEE43 pKa = 4.07PWSLTIDD50 pKa = 3.25ICQAYY55 pKa = 9.54FDD57 pKa = 5.2KK58 pKa = 11.13INNN61 pKa = 3.68

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y0T3F0|A0A1Y0T3F0_9CAUD Uncharacterized protein OS=Pseudomonas phage Noxifer OX=2006684 GN=NOXIFER_242 PE=4 SV=1
MM1 pKa = 7.49ARR3 pKa = 11.84SRR5 pKa = 11.84NNGKK9 pKa = 9.6RR10 pKa = 11.84CGFEE14 pKa = 3.65YY15 pKa = 10.15WSRR18 pKa = 11.84RR19 pKa = 11.84PNCNGAVGKK28 pKa = 8.74FAKK31 pKa = 10.3RR32 pKa = 11.84IIHH35 pKa = 4.72GQEE38 pKa = 3.49RR39 pKa = 11.84IIAKK43 pKa = 9.97DD44 pKa = 3.31LTRR47 pKa = 11.84GEE49 pKa = 4.11VNAYY53 pKa = 10.06FSEE56 pKa = 4.34

Molecular weight: 6.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

334

0

334

86896

38

2318

260.2

29.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.432 ± 0.168

0.91 ± 0.049

6.356 ± 0.102

6.318 ± 0.125

3.862 ± 0.077

6.794 ± 0.186

2.272 ± 0.083

5.473 ± 0.084

5.115 ± 0.164

8.799 ± 0.114

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.94 ± 0.067

4.566 ± 0.081

4.665 ± 0.109

3.785 ± 0.076

5.456 ± 0.096

5.385 ± 0.088

6.258 ± 0.108

7.356 ± 0.116

1.373 ± 0.06

3.884 ± 0.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski