Bean leafroll virus (BLRV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Luteovirus

Average proteome isoelectric point is 7.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8V2F8|Q8V2F8_BLRV RNA dependent RNA polymerase 1 OS=Bean leafroll virus OX=12041 GN=BLRV_gp2 PE=4 SV=1
MM1 pKa = 7.47FNFDD5 pKa = 3.87SLITASSRR13 pKa = 11.84VVKK16 pKa = 10.53DD17 pKa = 5.52FIHH20 pKa = 6.26FCYY23 pKa = 10.38NRR25 pKa = 11.84ARR27 pKa = 11.84SVYY30 pKa = 9.58CALKK34 pKa = 9.25RR35 pKa = 11.84WLWEE39 pKa = 3.84LQGKK43 pKa = 9.37FDD45 pKa = 3.54AHH47 pKa = 6.06NAFVDD52 pKa = 4.01LCYY55 pKa = 10.83DD56 pKa = 3.3AMYY59 pKa = 10.46GIEE62 pKa = 4.26KK63 pKa = 10.41FEE65 pKa = 4.18EE66 pKa = 4.16EE67 pKa = 4.0LAEE70 pKa = 4.42EE71 pKa = 4.31YY72 pKa = 10.25TLAEE76 pKa = 4.24SEE78 pKa = 4.39VALAEE83 pKa = 3.75CHH85 pKa = 5.29YY86 pKa = 10.53RR87 pKa = 11.84YY88 pKa = 9.72LVSNGAPLTHH98 pKa = 6.8WPVPCPPPGAHH109 pKa = 7.13RR110 pKa = 11.84SIDD113 pKa = 3.8DD114 pKa = 3.81YY115 pKa = 11.64EE116 pKa = 5.93DD117 pKa = 3.71SVDD120 pKa = 3.37EE121 pKa = 4.55AGAFEE126 pKa = 4.3EE127 pKa = 5.11VIPSVPKK134 pKa = 10.36PEE136 pKa = 5.46PIPSKK141 pKa = 10.54DD142 pKa = 3.12GKK144 pKa = 10.38KK145 pKa = 10.17DD146 pKa = 3.4HH147 pKa = 6.91SKK149 pKa = 11.14SNPFRR154 pKa = 11.84VKK156 pKa = 10.95AFINLVKK163 pKa = 10.7AKK165 pKa = 9.87EE166 pKa = 4.02VLQATEE172 pKa = 3.79QKK174 pKa = 10.2IKK176 pKa = 10.71VVYY179 pKa = 9.72EE180 pKa = 3.99EE181 pKa = 5.15EE182 pKa = 4.05IGEE185 pKa = 4.49SPFGRR190 pKa = 11.84WFNTLPSRR198 pKa = 11.84MHH200 pKa = 5.9YY201 pKa = 9.86IKK203 pKa = 10.68RR204 pKa = 11.84ALKK207 pKa = 10.02KK208 pKa = 9.95RR209 pKa = 11.84AVNAKK214 pKa = 9.01RR215 pKa = 11.84AKK217 pKa = 10.21HH218 pKa = 4.56VTSMMKK224 pKa = 9.67EE225 pKa = 4.04VNVIPDD231 pKa = 3.94FVACCEE237 pKa = 4.3VVQVPTGEE245 pKa = 4.27MIPPKK250 pKa = 10.18KK251 pKa = 10.04NEE253 pKa = 3.95HH254 pKa = 6.31GEE256 pKa = 4.25DD257 pKa = 3.49VGEE260 pKa = 4.09PTPKK264 pKa = 9.12TKK266 pKa = 9.55MVRR269 pKa = 11.84RR270 pKa = 11.84VLPEE274 pKa = 3.59CAAEE278 pKa = 3.8ARR280 pKa = 11.84SYY282 pKa = 10.58IRR284 pKa = 11.84QHH286 pKa = 5.45IRR288 pKa = 11.84NNNMRR293 pKa = 11.84LIDD296 pKa = 4.03GTDD299 pKa = 3.23VAHH302 pKa = 6.24ATINRR307 pKa = 11.84YY308 pKa = 8.19ALKK311 pKa = 10.33FCEE314 pKa = 4.8EE315 pKa = 4.8LDD317 pKa = 3.88LDD319 pKa = 4.38LPSTTMLVDD328 pKa = 3.33YY329 pKa = 10.85AMTMVPLPLKK339 pKa = 10.75NDD341 pKa = 3.15IEE343 pKa = 4.3RR344 pKa = 11.84AKK346 pKa = 10.32IVHH349 pKa = 6.13SPNARR354 pKa = 11.84RR355 pKa = 11.84IRR357 pKa = 11.84DD358 pKa = 3.66EE359 pKa = 4.46LDD361 pKa = 3.42CLTSSVFF368 pKa = 3.37

Molecular weight: 42.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8V2F7|Q8V2F7_BLRV RNA-directed RNA polymerase OS=Bean leafroll virus OX=12041 PE=4 SV=1
MM1 pKa = 6.89VARR4 pKa = 11.84GKK6 pKa = 10.36RR7 pKa = 11.84VVVRR11 pKa = 11.84QLQTRR16 pKa = 11.84ARR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LPVVLATAPVRR31 pKa = 11.84PQRR34 pKa = 11.84KK35 pKa = 8.02RR36 pKa = 11.84RR37 pKa = 11.84QRR39 pKa = 11.84GRR41 pKa = 11.84NNKK44 pKa = 9.24SRR46 pKa = 11.84GGNGFARR53 pKa = 11.84RR54 pKa = 11.84SSQVHH59 pKa = 5.03EE60 pKa = 4.61FVFSKK65 pKa = 11.2DD66 pKa = 3.54NLNGNSKK73 pKa = 10.76GSITFGPSLSEE84 pKa = 4.24CKK86 pKa = 10.04PLADD90 pKa = 5.55GILKK94 pKa = 10.25AYY96 pKa = 10.27HH97 pKa = 7.06EE98 pKa = 4.47YY99 pKa = 11.01NITNVEE105 pKa = 3.75LAYY108 pKa = 9.16ITEE111 pKa = 4.46ASSTSSGSIAYY122 pKa = 9.49EE123 pKa = 3.79LDD125 pKa = 3.12PHH127 pKa = 7.07LKK129 pKa = 8.52NTTIQSKK136 pKa = 10.11INKK139 pKa = 9.32FSITKK144 pKa = 9.48SEE146 pKa = 3.92KK147 pKa = 10.18KK148 pKa = 10.12KK149 pKa = 10.63FSRR152 pKa = 11.84KK153 pKa = 9.59AINGQAWHH161 pKa = 7.0DD162 pKa = 3.6TSEE165 pKa = 3.98DD166 pKa = 3.25QFRR169 pKa = 11.84ILYY172 pKa = 8.67EE173 pKa = 4.14GNGDD177 pKa = 3.66AKK179 pKa = 10.36IAGSFRR185 pKa = 11.84VTIKK189 pKa = 10.95VLTQNPKK196 pKa = 10.14

Molecular weight: 21.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2329

142

901

465.8

52.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.612 ± 0.597

1.632 ± 0.615

5.195 ± 0.639

7.085 ± 0.763

3.993 ± 0.532

4.852 ± 0.52

2.319 ± 0.315

5.067 ± 0.359

7.17 ± 0.791

7.128 ± 0.597

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.619

5.024 ± 0.487

6.483 ± 0.77

3.091 ± 0.541

7.256 ± 1.1

8.416 ± 1.908

5.067 ± 0.978

6.999 ± 0.933

1.03 ± 0.128

3.263 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski