Pseudomonas phage Epa33

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Hollowayvirus; unclassified Hollowayvirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G9LK17|A0A6G9LK17_9CAUD Uncharacterized protein OS=Pseudomonas phage Epa33 OX=2719194 GN=33_00005 PE=4 SV=1
MM1 pKa = 5.6WTYY4 pKa = 10.91RR5 pKa = 11.84EE6 pKa = 3.97RR7 pKa = 11.84RR8 pKa = 11.84NRR10 pKa = 11.84AAFSNAQHH18 pKa = 6.38AWDD21 pKa = 4.68FARR24 pKa = 11.84DD25 pKa = 3.96PLWDD29 pKa = 3.67QPDD32 pKa = 4.13PEE34 pKa = 6.12PEE36 pKa = 4.63DD37 pKa = 4.19EE38 pKa = 4.35NQEE41 pKa = 4.22DD42 pKa = 4.5DD43 pKa = 5.05DD44 pKa = 4.97GLGEE48 pKa = 4.05

Molecular weight: 5.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G9LJS3|A0A6G9LJS3_9CAUD Capsid protein OS=Pseudomonas phage Epa33 OX=2719194 GN=33_00045 PE=4 SV=1
MM1 pKa = 7.11NRR3 pKa = 11.84VHH5 pKa = 7.09IKK7 pKa = 9.55TFASEE12 pKa = 4.23HH13 pKa = 5.19GQSRR17 pKa = 11.84AAALLGITQGALSKK31 pKa = 10.51ALRR34 pKa = 11.84VGRR37 pKa = 11.84DD38 pKa = 2.95IYY40 pKa = 10.31VTAHH44 pKa = 6.91ADD46 pKa = 3.47GTFTAEE52 pKa = 4.16EE53 pKa = 4.5VRR55 pKa = 11.84TFPAQKK61 pKa = 10.15VRR63 pKa = 11.84FVSS66 pKa = 3.52

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

20324

29

3799

282.3

30.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.857 ± 0.571

0.777 ± 0.164

6.219 ± 0.197

6.17 ± 0.301

2.883 ± 0.146

7.794 ± 0.269

2.052 ± 0.182

4.227 ± 0.264

3.902 ± 0.278

8.128 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.143

3.051 ± 0.135

5.501 ± 0.361

5.097 ± 0.284

7.316 ± 0.312

5.466 ± 0.182

5.373 ± 0.294

6.687 ± 0.265

1.584 ± 0.158

2.544 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski