Osedax symbiont Rs2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; unclassified Oceanospirillaceae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4523 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S6GPC6|S6GPC6_9GAMM tRNA (guanine-N(7)-)-methyltransferase OS=Osedax symbiont Rs2 OX=1330035 GN=trmB PE=3 SV=1
MM1 pKa = 7.27NWDD4 pKa = 3.3LSKK7 pKa = 10.64IEE9 pKa = 4.6EE10 pKa = 4.39SLSQQSNWVVHH21 pKa = 5.78SEE23 pKa = 3.84NDD25 pKa = 3.45CLFITNEE32 pKa = 4.54DD33 pKa = 3.56GLDD36 pKa = 3.4AYY38 pKa = 10.87VAVSGAQIVVEE49 pKa = 4.63SILFASSSVADD60 pKa = 3.44VAGLNEE66 pKa = 4.66QILKK70 pKa = 7.12THH72 pKa = 5.67QVFPLTTIGITSVEE86 pKa = 3.75NDD88 pKa = 3.79DD89 pKa = 4.16YY90 pKa = 12.08YY91 pKa = 11.13MAFGALSSQSKK102 pKa = 9.68EE103 pKa = 3.47EE104 pKa = 4.13SIIIEE109 pKa = 4.22VEE111 pKa = 4.07TLFDD115 pKa = 3.5NVAAFLDD122 pKa = 4.08AYY124 pKa = 10.41EE125 pKa = 5.6DD126 pKa = 3.86YY127 pKa = 11.41LKK129 pKa = 11.16

Molecular weight: 14.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S6HTT3|S6HTT3_9GAMM RNA polymerase sigma factor RpoH OS=Osedax symbiont Rs2 OX=1330035 GN=rpoH PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.31SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.79KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4523

0

4523

1267671

37

6909

280.3

31.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.181 ± 0.053

1.194 ± 0.014

5.274 ± 0.035

5.349 ± 0.038

4.056 ± 0.027

6.569 ± 0.039

2.061 ± 0.019

6.916 ± 0.035

5.171 ± 0.034

11.054 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.021

4.177 ± 0.031

3.689 ± 0.024

5.371 ± 0.038

4.438 ± 0.035

7.318 ± 0.037

4.925 ± 0.027

6.59 ± 0.039

1.15 ± 0.013

2.953 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski