Osedax symbiont Rs2
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4523 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S6GPC6|S6GPC6_9GAMM tRNA (guanine-N(7)-)-methyltransferase OS=Osedax symbiont Rs2 OX=1330035 GN=trmB PE=3 SV=1
MM1 pKa = 7.27 NWDD4 pKa = 3.3 LSKK7 pKa = 10.64 IEE9 pKa = 4.6 EE10 pKa = 4.39 SLSQQSNWVVHH21 pKa = 5.78 SEE23 pKa = 3.84 NDD25 pKa = 3.45 CLFITNEE32 pKa = 4.54 DD33 pKa = 3.56 GLDD36 pKa = 3.4 AYY38 pKa = 10.87 VAVSGAQIVVEE49 pKa = 4.63 SILFASSSVADD60 pKa = 3.44 VAGLNEE66 pKa = 4.66 QILKK70 pKa = 7.12 THH72 pKa = 5.67 QVFPLTTIGITSVEE86 pKa = 3.75 NDD88 pKa = 3.79 DD89 pKa = 4.16 YY90 pKa = 12.08 YY91 pKa = 11.13 MAFGALSSQSKK102 pKa = 9.68 EE103 pKa = 3.47 EE104 pKa = 4.13 SIIIEE109 pKa = 4.22 VEE111 pKa = 4.07 TLFDD115 pKa = 3.5 NVAAFLDD122 pKa = 4.08 AYY124 pKa = 10.41 EE125 pKa = 5.6 DD126 pKa = 3.86 YY127 pKa = 11.41 LKK129 pKa = 11.16
Molecular weight: 14.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.77
IPC_protein 3.719
Toseland 3.528
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.554
Grimsley 3.439
Solomon 3.681
Lehninger 3.63
Nozaki 3.821
DTASelect 3.986
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.834
Patrickios 0.846
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.752
Protein with the highest isoelectric point:
>tr|S6HTT3|S6HTT3_9GAMM RNA polymerase sigma factor RpoH OS=Osedax symbiont Rs2 OX=1330035 GN=rpoH PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.31 SGRR28 pKa = 11.84 AVINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.1 GRR39 pKa = 11.84 KK40 pKa = 8.79 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4523
0
4523
1267671
37
6909
280.3
31.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.181 ± 0.053
1.194 ± 0.014
5.274 ± 0.035
5.349 ± 0.038
4.056 ± 0.027
6.569 ± 0.039
2.061 ± 0.019
6.916 ± 0.035
5.171 ± 0.034
11.054 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.566 ± 0.021
4.177 ± 0.031
3.689 ± 0.024
5.371 ± 0.038
4.438 ± 0.035
7.318 ± 0.037
4.925 ± 0.027
6.59 ± 0.039
1.15 ± 0.013
2.953 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here