Wheat dwarf India virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0E0B6|I0E0B6_9GEMI Replication-associated protein OS=Wheat dwarf India virus OX=1174526 GN=RepA PE=3 SV=1
MM1 pKa = 7.16SQTSAEE7 pKa = 4.15NNSANPKK14 pKa = 9.85ASSSTFRR21 pKa = 11.84YY22 pKa = 9.66RR23 pKa = 11.84SNNCFLTFPHH33 pKa = 6.85CNSCPYY39 pKa = 11.32GMVQHH44 pKa = 6.51FWDD47 pKa = 6.2LISTWSPIYY56 pKa = 10.52AVASVEE62 pKa = 3.96LHH64 pKa = 6.31QDD66 pKa = 3.6GTPHH70 pKa = 7.04LHH72 pKa = 7.39ALLQTRR78 pKa = 11.84KK79 pKa = 9.99QISTNDD85 pKa = 3.09PHH87 pKa = 8.17FFDD90 pKa = 5.45FDD92 pKa = 3.48GHH94 pKa = 6.39HH95 pKa = 6.67PHH97 pKa = 6.45IQAAKK102 pKa = 10.2NPTLCRR108 pKa = 11.84DD109 pKa = 4.1YY110 pKa = 10.96ILKK113 pKa = 10.76GPITFSEE120 pKa = 4.21KK121 pKa = 10.4GAFIPRR127 pKa = 11.84GRR129 pKa = 11.84NAGTSPRR136 pKa = 11.84HH137 pKa = 4.7SNKK140 pKa = 9.68RR141 pKa = 11.84SRR143 pKa = 11.84DD144 pKa = 3.83DD145 pKa = 3.16IMKK148 pKa = 10.33DD149 pKa = 2.87IIEE152 pKa = 4.36NSTNKK157 pKa = 9.72SDD159 pKa = 3.82YY160 pKa = 10.04LSKK163 pKa = 10.55VRR165 pKa = 11.84RR166 pKa = 11.84NFPYY170 pKa = 10.46DD171 pKa = 3.17WATKK175 pKa = 10.05LYY177 pKa = 9.47NFEE180 pKa = 4.19YY181 pKa = 10.26SASKK185 pKa = 10.41LFPEE189 pKa = 4.14QQPEE193 pKa = 3.89YY194 pKa = 10.27SNPHH198 pKa = 5.63GQSVPDD204 pKa = 3.91LYY206 pKa = 11.03CYY208 pKa = 7.88EE209 pKa = 5.18TIQDD213 pKa = 4.18WIDD216 pKa = 3.54SNLFQVHH223 pKa = 6.81PYY225 pKa = 10.52CYY227 pKa = 9.48MLNTPTINSVAEE239 pKa = 4.03AVQEE243 pKa = 4.11LTWLQEE249 pKa = 3.57NSRR252 pKa = 11.84IHH254 pKa = 6.19LQEE257 pKa = 3.56QGQRR261 pKa = 11.84ASTSSVQQGPEE272 pKa = 3.86NLHH275 pKa = 6.18GQGPWADD282 pKa = 3.62TTTGRR287 pKa = 11.84IMM289 pKa = 4.33

Molecular weight: 33.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0E0B3|I0E0B3_9GEMI Movement protein OS=Wheat dwarf India virus OX=1174526 GN=V1 PE=3 SV=1
MM1 pKa = 7.06SQVKK5 pKa = 9.91KK6 pKa = 8.96RR7 pKa = 11.84TRR9 pKa = 11.84RR10 pKa = 11.84GDD12 pKa = 3.32EE13 pKa = 4.14VEE15 pKa = 3.38WSKK18 pKa = 11.15RR19 pKa = 11.84KK20 pKa = 9.64RR21 pKa = 11.84LRR23 pKa = 11.84SSGSKK28 pKa = 10.74GSATKK33 pKa = 10.25LRR35 pKa = 11.84KK36 pKa = 9.92LPVRR40 pKa = 11.84PSLQIQSLYY49 pKa = 9.24YY50 pKa = 10.43TLTTYY55 pKa = 11.11VDD57 pKa = 4.65FKK59 pKa = 11.03TGGEE63 pKa = 4.27SYY65 pKa = 11.47LMASYY70 pKa = 11.26ARR72 pKa = 11.84GSDD75 pKa = 2.92EE76 pKa = 4.93GEE78 pKa = 3.7RR79 pKa = 11.84HH80 pKa = 5.31TNEE83 pKa = 3.34TMTYY87 pKa = 9.1KK88 pKa = 10.78AALNLHH94 pKa = 6.77LSASSDD100 pKa = 2.91ACKK103 pKa = 10.58YY104 pKa = 10.45SNTGTGCIWLIYY116 pKa = 9.59DD117 pKa = 4.79AQPTGNKK124 pKa = 9.14PSPKK128 pKa = 10.14DD129 pKa = 2.95IFGYY133 pKa = 10.22SDD135 pKa = 4.65SLNSWPTTWMVSRR148 pKa = 11.84EE149 pKa = 3.87QCHH152 pKa = 5.29RR153 pKa = 11.84FVVKK157 pKa = 10.48RR158 pKa = 11.84RR159 pKa = 11.84WTFTLEE165 pKa = 4.16TNGRR169 pKa = 11.84LASDD173 pKa = 4.03VPPSNVVWPPCKK185 pKa = 10.05KK186 pKa = 10.47SINFHH191 pKa = 6.3KK192 pKa = 9.94FAKK195 pKa = 9.91GLGVRR200 pKa = 11.84TEE202 pKa = 4.24WKK204 pKa = 10.07NKK206 pKa = 7.1TDD208 pKa = 3.57GGIGSIKK215 pKa = 10.24KK216 pKa = 8.67GALYY220 pKa = 9.5FVIAPGNGVEE230 pKa = 4.03GRR232 pKa = 11.84MFGTIRR238 pKa = 11.84MYY240 pKa = 10.66FKK242 pKa = 11.0SIGNQQ247 pKa = 3.0

Molecular weight: 27.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

999

106

357

249.8

28.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.906 ± 0.582

1.802 ± 0.232

4.905 ± 0.445

4.304 ± 0.109

4.705 ± 0.393

6.106 ± 1.017

3.103 ± 0.693

5.606 ± 0.514

5.506 ± 0.977

6.106 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.902 ± 0.152

5.706 ± 0.654

7.007 ± 0.754

4.505 ± 0.763

5.706 ± 0.728

9.109 ± 0.532

6.607 ± 0.499

4.204 ± 0.486

2.302 ± 0.139

4.905 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski