Cowpea polerovirus 1
Average proteome isoelectric point is 8.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I9W791|A0A1I9W791_9LUTE Serine protease OS=Cowpea polerovirus 1 OX=1913124 PE=4 SV=1
MM1 pKa = 7.07 TSSVVLFFFFLLSCVGSTSTMPGVLTGQHH30 pKa = 6.28 SLDD33 pKa = 4.83 FPISWEE39 pKa = 4.15 LPWNQTSVEE48 pKa = 4.03 HH49 pKa = 6.99 RR50 pKa = 11.84 GFLEE54 pKa = 4.62 PPTPPAKK61 pKa = 9.56 TEE63 pKa = 3.94 QYY65 pKa = 10.93 VEE67 pKa = 3.99 RR68 pKa = 11.84 TYY70 pKa = 11.49 SALALGLWEE79 pKa = 5.24 LLLSDD84 pKa = 3.66 TQKK87 pKa = 10.8 YY88 pKa = 8.53 CQEE91 pKa = 3.77 GDD93 pKa = 3.81 IILQHH98 pKa = 5.46 SWQYY102 pKa = 11.43 ACEE105 pKa = 4.21 TFNKK109 pKa = 6.92 TTLILFEE116 pKa = 4.74 GVLEE120 pKa = 3.91 LLIRR124 pKa = 11.84 VWSFVIFAIFYY135 pKa = 5.99 TAWRR139 pKa = 11.84 FITQWTMHH147 pKa = 6.77 ALLLLCLGLLTKK159 pKa = 10.6 ISIRR163 pKa = 11.84 TLRR166 pKa = 11.84 FVLGDD171 pKa = 3.45 WLVSLIAALIVAIIKK186 pKa = 9.4 LPWTCFKK193 pKa = 10.6 TVFFKK198 pKa = 10.77 PFSGIKK204 pKa = 9.92 PEE206 pKa = 3.96 MAVKK210 pKa = 10.55 GFVTHH215 pKa = 7.56 DD216 pKa = 2.94 IHH218 pKa = 6.44 MKK220 pKa = 9.68 PPRR223 pKa = 11.84 DD224 pKa = 3.87 SQLLMAWDD232 pKa = 4.89 DD233 pKa = 4.01 GSHH236 pKa = 6.65 AGYY239 pKa = 8.18 ATCIRR244 pKa = 11.84 LYY246 pKa = 10.67 NGTNALVTAAHH257 pKa = 6.14 CRR259 pKa = 11.84 KK260 pKa = 9.94 HH261 pKa = 5.84 EE262 pKa = 4.17 GFVVSTKK269 pKa = 9.2 TKK271 pKa = 10.5 NRR273 pKa = 11.84 IPINAFKK280 pKa = 10.03 PLCVDD285 pKa = 3.87 VKK287 pKa = 11.36 NDD289 pKa = 3.2 FALFEE294 pKa = 5.04 GPMEE298 pKa = 4.16 WEE300 pKa = 4.08 SLLGCKK306 pKa = 9.48 AVAATPMYY314 pKa = 10.56 LIRR317 pKa = 11.84 KK318 pKa = 8.75 GSASHH323 pKa = 5.23 YY324 pKa = 9.43 RR325 pKa = 11.84 YY326 pKa = 10.96 VNGEE330 pKa = 3.52 WFQRR334 pKa = 11.84 YY335 pKa = 8.02 AQIGEE340 pKa = 4.34 IVSPAHH346 pKa = 5.48 SNKK349 pKa = 9.9 YY350 pKa = 9.81 ALQVLSNTDD359 pKa = 3.35 EE360 pKa = 4.55 GDD362 pKa = 3.21 SGAGYY367 pKa = 9.93 FVGKK371 pKa = 9.95 NLVGIHH377 pKa = 6.53 LGASLTDD384 pKa = 3.23 NHH386 pKa = 6.44 NAMLPILPIPGLTIPDD402 pKa = 3.79 YY403 pKa = 10.79 QLEE406 pKa = 4.44 TTAPEE411 pKa = 4.36 GKK413 pKa = 10.1 VFTSSIYY420 pKa = 10.7 EE421 pKa = 4.03 EE422 pKa = 4.01 ALKK425 pKa = 9.8 ATEE428 pKa = 3.83 RR429 pKa = 11.84 TMKK432 pKa = 10.66 FIANLRR438 pKa = 11.84 SKK440 pKa = 10.82 GKK442 pKa = 9.35 EE443 pKa = 3.68 VWSDD447 pKa = 3.5 LVDD450 pKa = 5.21 DD451 pKa = 5.59 LDD453 pKa = 4.76 FDD455 pKa = 4.33 PEE457 pKa = 3.95 AAPRR461 pKa = 11.84 VHH463 pKa = 6.98 PEE465 pKa = 3.53 SSAKK469 pKa = 10.29 RR470 pKa = 11.84 SGKK473 pKa = 9.01 RR474 pKa = 11.84 QARR477 pKa = 11.84 HH478 pKa = 5.53 RR479 pKa = 11.84 PRR481 pKa = 11.84 TNRR484 pKa = 11.84 QGRR487 pKa = 11.84 TLTRR491 pKa = 11.84 LKK493 pKa = 10.62 HH494 pKa = 5.2 PYY496 pKa = 9.27 NRR498 pKa = 11.84 YY499 pKa = 9.02 GYY501 pKa = 10.48 KK502 pKa = 9.88 LQFYY506 pKa = 10.23 AGSDD510 pKa = 3.66 CKK512 pKa = 11.1 GGGGEE517 pKa = 4.19 DD518 pKa = 2.43 RR519 pKa = 11.84 HH520 pKa = 5.87 RR521 pKa = 11.84 FHH523 pKa = 7.57 RR524 pKa = 11.84 EE525 pKa = 3.34 SGDD528 pKa = 3.61 RR529 pKa = 11.84 QGVDD533 pKa = 3.2 QDD535 pKa = 3.67 HH536 pKa = 6.04 QAPAKK541 pKa = 9.75 TKK543 pKa = 8.69 AQQAEE548 pKa = 4.76 EE549 pKa = 4.34 YY550 pKa = 9.31 CAYY553 pKa = 10.22 FASLYY558 pKa = 10.01 RR559 pKa = 11.84 WSEE562 pKa = 3.77 NPAPSEE568 pKa = 3.91 APGFKK573 pKa = 9.81 TVGRR577 pKa = 11.84 LPKK580 pKa = 10.6 YY581 pKa = 10.59 YY582 pKa = 9.89 FAKK585 pKa = 10.53 QKK587 pKa = 10.97 GEE589 pKa = 4.03 PEE591 pKa = 4.03 FGRR594 pKa = 11.84 EE595 pKa = 4.13 LVRR598 pKa = 11.84 KK599 pKa = 9.37 HH600 pKa = 6.42 PYY602 pKa = 10.02 LATKK606 pKa = 9.97 TEE608 pKa = 4.42 GFGWPQFGAEE618 pKa = 4.42 AEE620 pKa = 4.3 LTSLILQAGRR630 pKa = 11.84 WRR632 pKa = 11.84 SRR634 pKa = 11.84 AAGTEE639 pKa = 4.08 IPSSQARR646 pKa = 11.84 EE647 pKa = 3.6 RR648 pKa = 11.84 VINRR652 pKa = 11.84 TVQAYY657 pKa = 8.45 QAVRR661 pKa = 11.84 TPCPIIASSGKK672 pKa = 10.35 LEE674 pKa = 3.86 WSTFLLGMEE683 pKa = 3.93 EE684 pKa = 4.66 AIRR687 pKa = 11.84 SLEE690 pKa = 3.8 LDD692 pKa = 3.36 AGVGVPYY699 pKa = 10.37 ISLKK703 pKa = 10.59 RR704 pKa = 11.84 PTHH707 pKa = 6.27 RR708 pKa = 11.84 GMIEE712 pKa = 4.17 DD713 pKa = 4.12 PQLLPVVTHH722 pKa = 6.51 LVFSRR727 pKa = 11.84 LQKK730 pKa = 9.46 MLEE733 pKa = 3.82 MDD735 pKa = 4.79 FEE737 pKa = 5.2 SMTPVEE743 pKa = 4.31 LVQNGLCDD751 pKa = 4.94 PIRR754 pKa = 11.84 LFVKK758 pKa = 10.71 GEE760 pKa = 3.78 PHH762 pKa = 6.67 KK763 pKa = 10.54 KK764 pKa = 10.27 AKK766 pKa = 10.51 LEE768 pKa = 3.89 EE769 pKa = 3.98 GRR771 pKa = 11.84 YY772 pKa = 8.82 RR773 pKa = 11.84 LIMSVSLVDD782 pKa = 3.47 QLVARR787 pKa = 11.84 LLFQDD792 pKa = 3.29 QNKK795 pKa = 9.87 RR796 pKa = 11.84 EE797 pKa = 4.07 IEE799 pKa = 3.87 LWRR802 pKa = 11.84 AVPSKK807 pKa = 10.57 PGFGLSTDD815 pKa = 3.55 SQVQEE820 pKa = 4.25 FVEE823 pKa = 4.39 VLARR827 pKa = 11.84 QVGLPSEE834 pKa = 4.32 EE835 pKa = 5.0 VITSWRR841 pKa = 11.84 NHH843 pKa = 7.42 LIPTDD848 pKa = 3.56 CSGFDD853 pKa = 3.36 WSVQDD858 pKa = 4.76 WMLQDD863 pKa = 3.34 EE864 pKa = 4.66 MEE866 pKa = 4.23 VRR868 pKa = 11.84 NRR870 pKa = 11.84 LTLNNNDD877 pKa = 3.58 CLRR880 pKa = 11.84 RR881 pKa = 11.84 LRR883 pKa = 11.84 SGWLKK888 pKa = 10.64 CLSNSVLCLSDD899 pKa = 3.47 GTLLSQEE906 pKa = 4.06 FPGIQKK912 pKa = 10.23 SGSYY916 pKa = 8.27 NTSSSNSRR924 pKa = 11.84 IRR926 pKa = 11.84 VMCAFHH932 pKa = 6.75 TGASWCMAMGDD943 pKa = 4.04 DD944 pKa = 4.94 ALEE947 pKa = 4.37 SVDD950 pKa = 4.53 GDD952 pKa = 3.57 LSRR955 pKa = 11.84 YY956 pKa = 9.71 SEE958 pKa = 4.69 LGLKK962 pKa = 8.97 VEE964 pKa = 4.78 RR965 pKa = 11.84 SEE967 pKa = 4.0 QLEE970 pKa = 4.37 FCSHH974 pKa = 6.15 IFEE977 pKa = 4.75 SPSRR981 pKa = 11.84 AIPVNANKK989 pKa = 9.34 MLYY992 pKa = 10.03 KK993 pKa = 10.62 LIYY996 pKa = 9.68 GYY998 pKa = 11.09 NPGSGNVEE1006 pKa = 4.21 VITNYY1011 pKa = 8.68 LTACLSILNEE1021 pKa = 3.96 LRR1023 pKa = 11.84 HH1024 pKa = 6.26 DD1025 pKa = 4.13 PSLVEE1030 pKa = 3.56 QLYY1033 pKa = 10.34 RR1034 pKa = 11.84 DD1035 pKa = 3.58 LFVPVEE1041 pKa = 3.93 AQKK1044 pKa = 11.31 NSGRR1048 pKa = 11.84 HH1049 pKa = 3.86
Molecular weight: 118.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.86
IPC2_protein 7.176
IPC_protein 7.029
Toseland 6.868
ProMoST 7.571
Dawson 7.688
Bjellqvist 8.083
Wikipedia 7.614
Rodwell 7.702
Grimsley 6.956
Solomon 7.717
Lehninger 7.732
Nozaki 8.317
DTASelect 7.878
Thurlkill 7.922
EMBOSS 7.936
Sillero 8.214
Patrickios 4.279
IPC_peptide 7.717
IPC2_peptide 7.322
IPC2.peptide.svr19 7.138
Protein with the highest isoelectric point:
>tr|A0A1I9W794|A0A1I9W794_9LUTE Movement protein OS=Cowpea polerovirus 1 OX=1913124 PE=3 SV=1
MM1 pKa = 6.59 NTVVVKK7 pKa = 10.48 RR8 pKa = 11.84 RR9 pKa = 11.84 NGGPNRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 NRR19 pKa = 11.84 RR20 pKa = 11.84 AARR23 pKa = 11.84 SNTMVMVQAPRR34 pKa = 11.84 PTGRR38 pKa = 11.84 GGRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 NRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 PNRR51 pKa = 11.84 GGRR54 pKa = 11.84 NRR56 pKa = 11.84 GGSGEE61 pKa = 4.19 TFVFSKK67 pKa = 11.12 DD68 pKa = 3.51 NLSGSSSGAITFGPSLSDD86 pKa = 3.49 CPAFSSGILKK96 pKa = 10.26 AYY98 pKa = 10.18 HH99 pKa = 6.62 EE100 pKa = 4.74 YY101 pKa = 9.97 KK102 pKa = 10.18 ISMVKK107 pKa = 10.31 VEE109 pKa = 5.76 FISEE113 pKa = 4.07 AASTSSGSIAYY124 pKa = 9.43 EE125 pKa = 3.86 LDD127 pKa = 3.43 PHH129 pKa = 6.8 CKK131 pKa = 8.66 STSLQSYY138 pKa = 7.42 VNKK141 pKa = 10.44 FGITRR146 pKa = 11.84 NGQRR150 pKa = 11.84 TWTGRR155 pKa = 11.84 LINGVEE161 pKa = 3.91 WHH163 pKa = 7.08 DD164 pKa = 3.55 TTEE167 pKa = 3.86 DD168 pKa = 3.22 QFRR171 pKa = 11.84 ILYY174 pKa = 9.33 KK175 pKa = 10.99 GNGSSAIAGSFRR187 pKa = 11.84 ITIKK191 pKa = 10.84 CQTQNPKK198 pKa = 10.57
Molecular weight: 21.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.209
IPC2_protein 10.058
IPC_protein 11.286
Toseland 11.403
ProMoST 11.798
Dawson 11.433
Bjellqvist 11.359
Wikipedia 11.842
Rodwell 11.33
Grimsley 11.462
Solomon 11.842
Lehninger 11.754
Nozaki 11.403
DTASelect 11.359
Thurlkill 11.403
EMBOSS 11.886
Sillero 11.403
Patrickios 11.052
IPC_peptide 11.842
IPC2_peptide 10.804
IPC2.peptide.svr19 9.158
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3020
45
1049
431.4
48.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.987 ± 0.314
1.689 ± 0.161
4.603 ± 0.503
5.497 ± 0.518
4.305 ± 0.49
7.252 ± 0.573
2.152 ± 0.353
4.735 ± 0.347
4.868 ± 0.54
8.609 ± 1.309
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.954 ± 0.115
4.205 ± 0.552
6.126 ± 0.615
3.609 ± 0.27
7.417 ± 0.951
8.808 ± 0.628
6.093 ± 0.487
6.093 ± 0.326
1.854 ± 0.23
3.113 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here