Paramicrosporidium saccamoebae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Cryptomycota; Cryptomycota incertae sedis; Paramicrosporidium

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3754 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H9TMI5|A0A2H9TMI5_9FUNG Putative serine threonine protein kinase OS=Paramicrosporidium saccamoebae OX=1246581 GN=PSACC_01238 PE=3 SV=1
MM1 pKa = 7.61SPPGLIRR8 pKa = 11.84IKK10 pKa = 10.49RR11 pKa = 11.84SAEE14 pKa = 3.98DD15 pKa = 3.78PPLVALLCEE24 pKa = 4.35HH25 pKa = 7.57DD26 pKa = 5.11EE27 pKa = 4.28DD28 pKa = 3.99TLYY31 pKa = 10.52YY32 pKa = 10.83QLMHH36 pKa = 6.96HH37 pKa = 7.08DD38 pKa = 4.33EE39 pKa = 4.5SNKK42 pKa = 7.57TACVEE47 pKa = 3.89PVTLSEE53 pKa = 4.23ALEE56 pKa = 4.1QIKK59 pKa = 10.38IEE61 pKa = 4.06ALPVKK66 pKa = 9.55MDD68 pKa = 3.57EE69 pKa = 4.52VKK71 pKa = 11.13LEE73 pKa = 4.31DD74 pKa = 3.47EE75 pKa = 4.36TLDD78 pKa = 3.55GFVYY82 pKa = 10.33DD83 pKa = 4.16YY84 pKa = 11.18YY85 pKa = 10.83LHH87 pKa = 7.25KK88 pKa = 10.43STAPEE93 pKa = 3.7QLDD96 pKa = 3.39VSRR99 pKa = 11.84IDD101 pKa = 4.13EE102 pKa = 4.5EE103 pKa = 4.94DD104 pKa = 3.79SLWEE108 pKa = 4.92DD109 pKa = 4.8DD110 pKa = 4.07EE111 pKa = 5.73DD112 pKa = 5.75SNAEE116 pKa = 4.07DD117 pKa = 5.19YY118 pKa = 10.44YY119 pKa = 11.53QNDD122 pKa = 3.86YY123 pKa = 10.95PDD125 pKa = 4.6EE126 pKa = 6.13DD127 pKa = 5.25DD128 pKa = 5.55DD129 pKa = 5.64NSDD132 pKa = 3.37VTSNEE137 pKa = 4.83DD138 pKa = 2.83SDD140 pKa = 2.96HH141 pKa = 6.81WYY143 pKa = 10.39RR144 pKa = 11.84SGLSRR149 pKa = 11.84HH150 pKa = 6.48HH151 pKa = 7.26DD152 pKa = 3.5GCNSGFSDD160 pKa = 4.38DD161 pKa = 4.64DD162 pKa = 4.32AYY164 pKa = 11.12

Molecular weight: 18.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H9TJL1|A0A2H9TJL1_9FUNG Mitochondrial phosphate carrier protein 2 OS=Paramicrosporidium saccamoebae OX=1246581 GN=PSACC_02238 PE=3 SV=1
MM1 pKa = 7.53HH2 pKa = 7.75LNTFIIASIVPMALGFMSIPNRR24 pKa = 11.84WVRR27 pKa = 11.84HH28 pKa = 4.85CRR30 pKa = 11.84AGHH33 pKa = 4.81TRR35 pKa = 11.84AIRR38 pKa = 11.84LADD41 pKa = 3.94CDD43 pKa = 3.82EE44 pKa = 4.85LGTRR48 pKa = 11.84GMEE51 pKa = 3.78QCLMSTHH58 pKa = 6.91DD59 pKa = 3.51RR60 pKa = 11.84TLAALIRR67 pKa = 11.84RR68 pKa = 11.84CPNQLPKK75 pKa = 10.49FRR77 pKa = 11.84DD78 pKa = 3.38FDD80 pKa = 3.95RR81 pKa = 11.84FALRR85 pKa = 11.84LYY87 pKa = 10.75QSFLPRR93 pKa = 11.84AFRR96 pKa = 11.84AYY98 pKa = 9.32IQTRR102 pKa = 11.84CGQLSRR108 pKa = 11.84STLRR112 pKa = 11.84EE113 pKa = 3.83LLNSFVNNNSVPFVRR128 pKa = 11.84AVMQHH133 pKa = 5.59CVVGEE138 pKa = 4.1RR139 pKa = 11.84IWRR142 pKa = 11.84QFAQQARR149 pKa = 11.84LQGKK153 pKa = 9.19NEE155 pKa = 4.02LLVVLRR161 pKa = 11.84AGPAGPVGQPGQSGQSGQSGQSGQLGQGAVQDD193 pKa = 4.17DD194 pKa = 4.66SKK196 pKa = 11.63NSWGMSYY203 pKa = 10.28SVKK206 pKa = 10.19RR207 pKa = 11.84DD208 pKa = 3.36VHH210 pKa = 5.4TMPSGEE216 pKa = 4.02RR217 pKa = 11.84FAVARR222 pKa = 11.84QEE224 pKa = 3.77ASKK227 pKa = 11.14DD228 pKa = 3.4GGIQPGALGQNGGLAAGTLGQSGLADD254 pKa = 3.5PTFGGSAQVNQQRR267 pKa = 11.84ATSKK271 pKa = 10.07RR272 pKa = 11.84AYY274 pKa = 10.63LKK276 pKa = 9.67VTTQQVASNLNILGRR291 pKa = 11.84LTKK294 pKa = 10.51AQASTFGMLSDD305 pKa = 4.19GCSALTEE312 pKa = 4.18SHH314 pKa = 6.76FRR316 pKa = 11.84QPTVSPWIVRR326 pKa = 11.84SLPQEE331 pKa = 4.03CFVRR335 pKa = 11.84IPPPAFSGLTPAMISQIKK353 pKa = 7.97WWPFVTRR360 pKa = 11.84DD361 pKa = 3.36QIRR364 pKa = 11.84YY365 pKa = 9.18VPTGDD370 pKa = 4.93AIRR373 pKa = 11.84ALPFDD378 pKa = 3.51QLGKK382 pKa = 10.59GRR384 pKa = 11.84QKK386 pKa = 10.9DD387 pKa = 3.88GEE389 pKa = 4.3DD390 pKa = 3.5RR391 pKa = 11.84EE392 pKa = 4.72HH393 pKa = 6.9PCWTITRR400 pKa = 11.84DD401 pKa = 3.26QMRR404 pKa = 11.84SIRR407 pKa = 11.84RR408 pKa = 11.84SKK410 pKa = 9.73TVFKK414 pKa = 10.01EE415 pKa = 3.57YY416 pKa = 10.65SRR418 pKa = 11.84RR419 pKa = 11.84CVKK422 pKa = 10.51SGASAVVVSMSSDD435 pKa = 3.18FRR437 pKa = 11.84FPLGGPPLHH446 pKa = 6.53KK447 pKa = 10.14QRR449 pKa = 11.84PNQYY453 pKa = 10.44ARR455 pKa = 11.84AEE457 pKa = 4.38DD458 pKa = 3.98III460 pKa = 5.03

Molecular weight: 50.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3754

0

3754

1669180

36

6083

444.6

49.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.805 ± 0.034

1.603 ± 0.016

5.41 ± 0.025

6.509 ± 0.037

3.965 ± 0.025

5.785 ± 0.035

2.352 ± 0.017

5.411 ± 0.026

5.222 ± 0.043

10.355 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.016

3.629 ± 0.018

5.066 ± 0.032

3.807 ± 0.022

6.146 ± 0.034

7.886 ± 0.041

5.76 ± 0.039

6.718 ± 0.028

1.24 ± 0.013

2.776 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski