Sphingobacteriales bacterium

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; unclassified Sphingobacteriales

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2778 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2ZPH4|A0A4Q2ZPH4_9SPHI T9SS type A sorting domain-containing protein OS=Sphingobacteriales bacterium OX=2044944 GN=EOP52_03460 PE=4 SV=1
MM1 pKa = 7.41TIEE4 pKa = 5.79DD5 pKa = 3.49IHH7 pKa = 9.05DD8 pKa = 4.52DD9 pKa = 3.74YY10 pKa = 11.89ALCDD14 pKa = 4.03GEE16 pKa = 4.22ILSYY20 pKa = 10.15TVDD23 pKa = 3.16VARR26 pKa = 11.84CSFAVRR32 pKa = 11.84LLVRR36 pKa = 11.84KK37 pKa = 10.05IIGKK41 pKa = 7.52QTFQEE46 pKa = 4.5CKK48 pKa = 9.1IEE50 pKa = 4.47LVFSEE55 pKa = 5.05CDD57 pKa = 3.44SIDD60 pKa = 3.04ISEE63 pKa = 4.79DD64 pKa = 2.92FRR66 pKa = 11.84TSGTYY71 pKa = 10.47SDD73 pKa = 3.23ITLAKK78 pKa = 10.4LSDD81 pKa = 3.34GTFYY85 pKa = 11.39LSLDD89 pKa = 3.36PYY91 pKa = 11.23GNFGEE96 pKa = 4.16PHH98 pKa = 6.52EE99 pKa = 4.57NDD101 pKa = 2.98NYY103 pKa = 10.73VIVGSSLALVLDD115 pKa = 3.9TGDD118 pKa = 3.55TFEE121 pKa = 5.53LSS123 pKa = 3.23

Molecular weight: 13.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2ZIH1|A0A4Q2ZIH1_9SPHI Uncharacterized protein (Fragment) OS=Sphingobacteriales bacterium OX=2044944 GN=EOP52_13940 PE=4 SV=1
MM1 pKa = 7.39GSHH4 pKa = 6.68YY5 pKa = 11.28SHH7 pKa = 7.59LSQTDD12 pKa = 3.13RR13 pKa = 11.84VAIQLLLQAGCSRR26 pKa = 11.84RR27 pKa = 11.84QIADD31 pKa = 3.2KK32 pKa = 11.15LGFSPSTICRR42 pKa = 11.84EE43 pKa = 4.17VIRR46 pKa = 11.84GKK48 pKa = 10.47SSPTALPDD56 pKa = 3.45SYY58 pKa = 11.1RR59 pKa = 11.84GATAQSRR66 pKa = 11.84SHH68 pKa = 6.02EE69 pKa = 4.09RR70 pKa = 11.84RR71 pKa = 11.84ADD73 pKa = 3.2AGVARR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 10.34LGTNLKK86 pKa = 7.97TPLWRR91 pKa = 11.84TVIDD95 pKa = 3.77GLRR98 pKa = 11.84CRR100 pKa = 11.84WSPEE104 pKa = 3.56QIACRR109 pKa = 11.84LRR111 pKa = 11.84DD112 pKa = 3.59MTSAAILPSSASPPLAVSHH131 pKa = 5.12EE132 pKa = 4.44TIYY135 pKa = 10.95GAIYY139 pKa = 10.49AMPRR143 pKa = 11.84GTLRR147 pKa = 11.84SEE149 pKa = 3.89LVEE152 pKa = 4.95LLRR155 pKa = 11.84QSHH158 pKa = 5.78KK159 pKa = 10.72VRR161 pKa = 11.84LPRR164 pKa = 11.84ARR166 pKa = 11.84GTEE169 pKa = 3.84RR170 pKa = 11.84KK171 pKa = 9.64ARR173 pKa = 11.84LPNMTSIALRR183 pKa = 11.84PPEE186 pKa = 3.8VAARR190 pKa = 11.84IVPGHH195 pKa = 6.32WEE197 pKa = 3.74GDD199 pKa = 4.04LIKK202 pKa = 10.91GAMNRR207 pKa = 11.84SSVGTLVEE215 pKa = 3.98RR216 pKa = 11.84LSRR219 pKa = 11.84YY220 pKa = 9.45VMLVKK225 pKa = 10.81LEE227 pKa = 4.29GNTAEE232 pKa = 5.69DD233 pKa = 3.18ILNGFRR239 pKa = 11.84RR240 pKa = 11.84RR241 pKa = 11.84LKK243 pKa = 10.53SIPEE247 pKa = 4.1SLRR250 pKa = 11.84KK251 pKa = 8.33TMTYY255 pKa = 10.74DD256 pKa = 2.99QGSEE260 pKa = 3.74MAMHH264 pKa = 7.05EE265 pKa = 4.61KK266 pKa = 10.74LSADD270 pKa = 3.37LNMDD274 pKa = 3.78IFFCDD279 pKa = 3.61PHH281 pKa = 8.38SPWQRR286 pKa = 11.84GSNEE290 pKa = 3.75NANGLVRR297 pKa = 11.84EE298 pKa = 4.13YY299 pKa = 10.92LPKK302 pKa = 10.79DD303 pKa = 3.42GLQNPARR310 pKa = 11.84RR311 pKa = 11.84AVCARR316 pKa = 11.84AFGLASS322 pKa = 3.31

Molecular weight: 35.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2778

0

2778

974968

21

3759

351.0

38.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.353 ± 0.056

0.894 ± 0.018

5.063 ± 0.033

5.212 ± 0.06

4.277 ± 0.033

7.579 ± 0.048

1.885 ± 0.026

5.569 ± 0.037

4.571 ± 0.048

9.994 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.22 ± 0.026

4.267 ± 0.055

4.706 ± 0.036

4.341 ± 0.034

5.39 ± 0.035

6.25 ± 0.053

6.756 ± 0.084

6.706 ± 0.037

1.261 ± 0.018

3.705 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski