Halospina denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Halospina

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3014 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V3ERE7|A0A4V3ERE7_9GAMM Cobyric acid synthase OS=Halospina denitrificans OX=332522 GN=cobQ PE=3 SV=1
MM1 pKa = 7.34NRR3 pKa = 11.84NLLAAAVAASVTAAAPSLAVASTNISAGVWASYY36 pKa = 10.6RR37 pKa = 11.84YY38 pKa = 8.54VTDD41 pKa = 3.25SDD43 pKa = 3.89FAAPAFNSDD52 pKa = 3.51LDD54 pKa = 4.01EE55 pKa = 4.45EE56 pKa = 4.72TGGDD60 pKa = 3.22IGRR63 pKa = 11.84EE64 pKa = 3.95SLILYY69 pKa = 9.6GDD71 pKa = 3.72HH72 pKa = 5.78TQEE75 pKa = 4.46DD76 pKa = 5.46GPWRR80 pKa = 11.84LSAEE84 pKa = 4.1VRR86 pKa = 11.84FGPGSFSDD94 pKa = 4.01PDD96 pKa = 3.81NNSTGSFTAVHH107 pKa = 6.39KK108 pKa = 10.38LWAGRR113 pKa = 11.84SIGEE117 pKa = 3.99NWDD120 pKa = 3.38MKK122 pKa = 10.12IGKK125 pKa = 9.27SEE127 pKa = 4.27VPFGWKK133 pKa = 7.79TVNFWPGDD141 pKa = 3.84LLLGGYY147 pKa = 9.34GDD149 pKa = 4.21QMDD152 pKa = 4.07VGAKK156 pKa = 7.99FTGDD160 pKa = 3.07VGGWDD165 pKa = 3.28LAAAYY170 pKa = 8.07YY171 pKa = 10.1HH172 pKa = 6.05QDD174 pKa = 3.14DD175 pKa = 3.96WGEE178 pKa = 4.13TSTDD182 pKa = 3.58TIDD185 pKa = 5.9DD186 pKa = 3.87NGHH189 pKa = 6.22WGSSTTYY196 pKa = 10.48RR197 pKa = 11.84KK198 pKa = 9.48IQTGVADD205 pKa = 4.12ASYY208 pKa = 10.12TFGSDD213 pKa = 2.88GGSSAFGLSAQAGKK227 pKa = 9.7LQDD230 pKa = 3.89LTDD233 pKa = 3.89HH234 pKa = 7.04ANACTTSCTDD244 pKa = 4.92LDD246 pKa = 4.61DD247 pKa = 5.1GSHH250 pKa = 6.38NALALYY256 pKa = 10.65YY257 pKa = 9.39EE258 pKa = 4.8GAYY261 pKa = 10.6GPFTAKK267 pKa = 10.65AEE269 pKa = 3.98AMQVNRR275 pKa = 11.84DD276 pKa = 3.66VPNVANDD283 pKa = 3.55IEE285 pKa = 4.3NTRR288 pKa = 11.84YY289 pKa = 10.2ALQGGYY295 pKa = 10.0SVGNWFMFLDD305 pKa = 3.97ATAANTDD312 pKa = 3.24TDD314 pKa = 4.18GNQADD319 pKa = 3.95TVYY322 pKa = 10.77AFSPGVTYY330 pKa = 10.29DD331 pKa = 3.89YY332 pKa = 11.38GPGWIYY338 pKa = 11.09LEE340 pKa = 4.05YY341 pKa = 10.5LKK343 pKa = 10.94SDD345 pKa = 3.35GDD347 pKa = 3.37IDD349 pKa = 4.66ANGDD353 pKa = 3.09IYY355 pKa = 11.14EE356 pKa = 4.44ADD358 pKa = 3.62FDD360 pKa = 4.67AMYY363 pKa = 9.89VTMDD367 pKa = 3.3YY368 pKa = 11.39YY369 pKa = 11.23FF370 pKa = 4.84

Molecular weight: 39.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R7K171|A0A4R7K171_9GAMM CBS domain-containing protein OS=Halospina denitrificans OX=332522 GN=DES49_0286 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATQNGRR28 pKa = 11.84QVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.55RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3014

0

3014

1010808

29

2461

335.4

37.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.576 ± 0.049

0.906 ± 0.015

6.058 ± 0.038

7.307 ± 0.049

3.619 ± 0.029

8.047 ± 0.039

2.366 ± 0.024

4.909 ± 0.029

2.994 ± 0.036

10.523 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.563 ± 0.02

3.14 ± 0.022

4.85 ± 0.031

3.957 ± 0.028

7.19 ± 0.042

5.803 ± 0.031

5.163 ± 0.033

7.006 ± 0.039

1.427 ± 0.019

2.596 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski