Tortoise microvirus 48

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W9P0|A0A4P8W9P0_9VIRU Uncharacterized protein OS=Tortoise microvirus 48 OX=2583152 PE=4 SV=1
MM1 pKa = 6.9KK2 pKa = 9.93TNVYY6 pKa = 10.43SIIDD10 pKa = 3.67SAYY13 pKa = 9.78HH14 pKa = 5.78RR15 pKa = 11.84VQGIVVAPNSSLAIRR30 pKa = 11.84DD31 pKa = 3.92VAPMLQRR38 pKa = 11.84VAPHH42 pKa = 6.47FEE44 pKa = 3.37EE45 pKa = 5.9DD46 pKa = 3.83MYY48 pKa = 11.12IEE50 pKa = 4.83CIGYY54 pKa = 10.2YY55 pKa = 10.45DD56 pKa = 5.45DD57 pKa = 5.91DD58 pKa = 5.21LSFHH62 pKa = 5.36VTSPEE67 pKa = 4.04VFPWTADD74 pKa = 3.48NPEE77 pKa = 4.2VPMKK81 pKa = 10.82ALTGKK86 pKa = 8.94DD87 pKa = 3.31TVII90 pKa = 4.35

Molecular weight: 10.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W6M6|A0A4P8W6M6_9VIRU Uncharacterized protein OS=Tortoise microvirus 48 OX=2583152 PE=4 SV=1
MM1 pKa = 7.48ACSSPFFLEE10 pKa = 4.06TVKK13 pKa = 10.55DD14 pKa = 3.71YY15 pKa = 11.08RR16 pKa = 11.84GRR18 pKa = 11.84PVPLACRR25 pKa = 11.84VCNTCRR31 pKa = 11.84ADD33 pKa = 3.82RR34 pKa = 11.84VSYY37 pKa = 8.76WEE39 pKa = 4.1RR40 pKa = 11.84RR41 pKa = 11.84AKK43 pKa = 10.82YY44 pKa = 8.62EE45 pKa = 3.93WNNSISSAFVTFTYY59 pKa = 10.93DD60 pKa = 3.19NDD62 pKa = 3.46NVPIGRR68 pKa = 11.84TGQQSLRR75 pKa = 11.84RR76 pKa = 11.84DD77 pKa = 3.55DD78 pKa = 3.4LHH80 pKa = 8.88RR81 pKa = 11.84YY82 pKa = 9.35IDD84 pKa = 3.83TLRR87 pKa = 11.84HH88 pKa = 5.71RR89 pKa = 11.84LKK91 pKa = 10.44KK92 pKa = 10.73DD93 pKa = 2.78KK94 pKa = 10.64VLPARR99 pKa = 11.84CIPNFSFIACGEE111 pKa = 4.18YY112 pKa = 10.44GDD114 pKa = 4.57SFGRR118 pKa = 11.84PHH120 pKa = 5.56YY121 pKa = 10.23HH122 pKa = 5.88VLFFGLDD129 pKa = 3.43FANCAKK135 pKa = 10.21YY136 pKa = 10.83LKK138 pKa = 9.46TSWHH142 pKa = 5.97NGSIKK147 pKa = 10.15VDD149 pKa = 3.64PVKK152 pKa = 10.94NGAIRR157 pKa = 11.84YY158 pKa = 6.96VISYY162 pKa = 8.12MSKK165 pKa = 9.76EE166 pKa = 4.12LYY168 pKa = 10.05GDD170 pKa = 3.65RR171 pKa = 11.84RR172 pKa = 11.84DD173 pKa = 3.59EE174 pKa = 5.44EE175 pKa = 4.46YY176 pKa = 10.95FDD178 pKa = 4.01WDD180 pKa = 3.11IEE182 pKa = 4.5APFLMCSRR190 pKa = 11.84GFGSGLFEE198 pKa = 4.06SQRR201 pKa = 11.84NNIRR205 pKa = 11.84EE206 pKa = 4.02YY207 pKa = 11.08GAMKK211 pKa = 10.47FGTKK215 pKa = 9.98FFSVPPYY222 pKa = 9.36WKK224 pKa = 10.62NKK226 pKa = 9.26FIDD229 pKa = 3.59MDD231 pKa = 3.72IEE233 pKa = 4.19KK234 pKa = 10.8ADD236 pKa = 3.43ARR238 pKa = 11.84YY239 pKa = 10.0EE240 pKa = 3.88NARR243 pKa = 11.84RR244 pKa = 11.84IQFNNWSANGGHH256 pKa = 6.57FGSPYY261 pKa = 8.88KK262 pKa = 10.88AQMQKK267 pKa = 8.9TKK269 pKa = 10.21IRR271 pKa = 11.84AEE273 pKa = 4.07SLYY276 pKa = 10.52HH277 pKa = 6.67DD278 pKa = 3.65MLMRR282 pKa = 11.84GSKK285 pKa = 9.49IGYY288 pKa = 7.92KK289 pKa = 9.98RR290 pKa = 11.84PVYY293 pKa = 9.67MYY295 pKa = 9.99GGSGSKK301 pKa = 10.4LVGLIEE307 pKa = 4.04

Molecular weight: 35.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1662

66

635

237.4

26.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.498 ± 0.941

1.083 ± 0.477

6.438 ± 1.098

4.091 ± 0.407

5.475 ± 1.003

6.318 ± 0.761

1.504 ± 0.377

6.619 ± 2.016

4.693 ± 0.718

9.146 ± 1.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.407 ± 0.376

4.994 ± 0.604

4.272 ± 0.987

4.031 ± 1.227

5.596 ± 0.868

10.168 ± 1.272

5.355 ± 0.721

5.054 ± 0.741

1.143 ± 0.329

5.114 ± 0.966
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski