Amino acid dipepetide frequency for Bacillus sp. FJAT-25509

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.501AlaAla: 4.501 ± 0.089
0.555AlaCys: 0.555 ± 0.02
2.814AlaAsp: 2.814 ± 0.051
3.479AlaGlu: 3.479 ± 0.052
3.108AlaPhe: 3.108 ± 0.058
4.565AlaGly: 4.565 ± 0.079
1.078AlaHis: 1.078 ± 0.03
5.909AlaIle: 5.909 ± 0.083
4.747AlaLys: 4.747 ± 0.073
6.276AlaLeu: 6.276 ± 0.087
1.684AlaMet: 1.684 ± 0.042
2.973AlaAsn: 2.973 ± 0.042
1.844AlaPro: 1.844 ± 0.045
1.744AlaGln: 1.744 ± 0.033
2.14AlaArg: 2.14 ± 0.047
3.927AlaSer: 3.927 ± 0.057
3.561AlaThr: 3.561 ± 0.074
4.548AlaVal: 4.548 ± 0.072
0.543AlaTrp: 0.543 ± 0.023
2.249AlaTyr: 2.249 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.416CysAla: 0.416 ± 0.02
0.104CysCys: 0.104 ± 0.009
0.378CysAsp: 0.378 ± 0.02
0.481CysGlu: 0.481 ± 0.021
0.398CysPhe: 0.398 ± 0.017
0.653CysGly: 0.653 ± 0.025
0.181CysHis: 0.181 ± 0.012
0.674CysIle: 0.674 ± 0.023
0.43CysLys: 0.43 ± 0.022
0.748CysLeu: 0.748 ± 0.021
0.178CysMet: 0.178 ± 0.012
0.395CysAsn: 0.395 ± 0.02
0.31CysPro: 0.31 ± 0.016
0.209CysGln: 0.209 ± 0.013
0.246CysArg: 0.246 ± 0.012
0.595CysSer: 0.595 ± 0.021
0.429CysThr: 0.429 ± 0.021
0.424CysVal: 0.424 ± 0.019
0.077CysTrp: 0.077 ± 0.008
0.284CysTyr: 0.284 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
2.981AspAla: 2.981 ± 0.063
0.403AspCys: 0.403 ± 0.019
2.323AspAsp: 2.323 ± 0.048
4.114AspGlu: 4.114 ± 0.065
2.577AspPhe: 2.577 ± 0.046
3.328AspGly: 3.328 ± 0.056
1.061AspHis: 1.061 ± 0.027
3.793AspIle: 3.793 ± 0.06
3.08AspLys: 3.08 ± 0.056
5.303AspLeu: 5.303 ± 0.069
1.058AspMet: 1.058 ± 0.029
1.863AspAsn: 1.863 ± 0.043
1.826AspPro: 1.826 ± 0.049
2.028AspGln: 2.028 ± 0.041
1.954AspArg: 1.954 ± 0.041
2.735AspSer: 2.735 ± 0.053
2.254AspThr: 2.254 ± 0.041
3.523AspVal: 3.523 ± 0.063
0.6AspTrp: 0.6 ± 0.02
2.193AspTyr: 2.193 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
4.371GluAla: 4.371 ± 0.061
0.416GluCys: 0.416 ± 0.02
3.369GluAsp: 3.369 ± 0.056
5.912GluGlu: 5.912 ± 0.097
2.717GluPhe: 2.717 ± 0.046
3.874GluGly: 3.874 ± 0.053
1.351GluHis: 1.351 ± 0.033
5.941GluIle: 5.941 ± 0.09
6.236GluLys: 6.236 ± 0.086
6.96GluLeu: 6.96 ± 0.09
1.979GluMet: 1.979 ± 0.046
4.117GluAsn: 4.117 ± 0.058
1.58GluPro: 1.58 ± 0.038
2.873GluGln: 2.873 ± 0.053
2.772GluArg: 2.772 ± 0.059
3.538GluSer: 3.538 ± 0.055
3.403GluThr: 3.403 ± 0.049
4.856GluVal: 4.856 ± 0.06
0.679GluTrp: 0.679 ± 0.022
2.352GluTyr: 2.352 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
2.929PheAla: 2.929 ± 0.06
0.417PheCys: 0.417 ± 0.02
2.467PheAsp: 2.467 ± 0.046
2.948PheGlu: 2.948 ± 0.054
2.565PhePhe: 2.565 ± 0.059
3.54PheGly: 3.54 ± 0.061
0.984PheHis: 0.984 ± 0.028
4.506PheIle: 4.506 ± 0.063
3.022PheLys: 3.022 ± 0.053
4.785PheLeu: 4.785 ± 0.084
1.129PheMet: 1.129 ± 0.032
2.517PheAsn: 2.517 ± 0.045
1.675PhePro: 1.675 ± 0.042
1.553PheGln: 1.553 ± 0.038
1.467PheArg: 1.467 ± 0.035
3.572PheSer: 3.572 ± 0.055
2.903PheThr: 2.903 ± 0.056
3.306PheVal: 3.306 ± 0.062
0.475PheTrp: 0.475 ± 0.023
1.894PheTyr: 1.894 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
4.515GlyAla: 4.515 ± 0.098
0.605GlyCys: 0.605 ± 0.025
2.95GlyAsp: 2.95 ± 0.049
3.975GlyGlu: 3.975 ± 0.056
3.418GlyPhe: 3.418 ± 0.055
4.509GlyGly: 4.509 ± 0.075
1.275GlyHis: 1.275 ± 0.034
6.356GlyIle: 6.356 ± 0.069
5.244GlyLys: 5.244 ± 0.067
6.433GlyLeu: 6.433 ± 0.082
1.796GlyMet: 1.796 ± 0.04
3.058GlyAsn: 3.058 ± 0.055
1.796GlyPro: 1.796 ± 0.06
1.992GlyGln: 1.992 ± 0.037
2.279GlyArg: 2.279 ± 0.047
3.999GlySer: 3.999 ± 0.066
4.083GlyThr: 4.083 ± 0.065
4.923GlyVal: 4.923 ± 0.073
0.722GlyTrp: 0.722 ± 0.027
2.807GlyTyr: 2.807 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
1.079HisAla: 1.079 ± 0.028
0.181HisCys: 0.181 ± 0.012
0.94HisAsp: 0.94 ± 0.028
1.276HisGlu: 1.276 ± 0.034
1.124HisPhe: 1.124 ± 0.031
1.342HisGly: 1.342 ± 0.036
0.583HisHis: 0.583 ± 0.027
1.499HisIle: 1.499 ± 0.034
1.083HisLys: 1.083 ± 0.031
1.976HisLeu: 1.976 ± 0.04
0.447HisMet: 0.447 ± 0.02
0.895HisAsn: 0.895 ± 0.031
1.018HisPro: 1.018 ± 0.027
0.719HisGln: 0.719 ± 0.025
0.714HisArg: 0.714 ± 0.025
1.292HisSer: 1.292 ± 0.033
0.983HisThr: 0.983 ± 0.029
1.194HisVal: 1.194 ± 0.032
0.194HisTrp: 0.194 ± 0.011
0.828HisTyr: 0.828 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.934IleAla: 5.934 ± 0.089
0.86IleCys: 0.86 ± 0.026
4.598IleAsp: 4.598 ± 0.07
6.195IleGlu: 6.195 ± 0.092
3.812IlePhe: 3.812 ± 0.071
6.399IleGly: 6.399 ± 0.078
1.696IleHis: 1.696 ± 0.039
7.048IleIle: 7.048 ± 0.114
5.855IleLys: 5.855 ± 0.07
8.063IleLeu: 8.063 ± 0.115
1.811IleMet: 1.811 ± 0.039
4.414IleAsn: 4.414 ± 0.057
3.562IlePro: 3.562 ± 0.053
3.083IleGln: 3.083 ± 0.05
2.934IleArg: 2.934 ± 0.051
6.108IleSer: 6.108 ± 0.074
4.786IleThr: 4.786 ± 0.072
6.013IleVal: 6.013 ± 0.076
0.705IleTrp: 0.705 ± 0.027
2.723IleTyr: 2.723 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
4.333LysAla: 4.333 ± 0.069
0.362LysCys: 0.362 ± 0.018
4.217LysAsp: 4.217 ± 0.067
6.701LysGlu: 6.701 ± 0.077
2.589LysPhe: 2.589 ± 0.046
4.546LysGly: 4.546 ± 0.065
1.284LysHis: 1.284 ± 0.03
5.875LysIle: 5.875 ± 0.071
6.405LysLys: 6.405 ± 0.078
6.644LysLeu: 6.644 ± 0.08
2.337LysMet: 2.337 ± 0.042
4.655LysAsn: 4.655 ± 0.072
2.267LysPro: 2.267 ± 0.049
3.085LysGln: 3.085 ± 0.053
3.054LysArg: 3.054 ± 0.047
4.263LysSer: 4.263 ± 0.058
3.984LysThr: 3.984 ± 0.058
5.22LysVal: 5.22 ± 0.074
0.803LysTrp: 0.803 ± 0.023
2.656LysTyr: 2.656 ± 0.046
0.0LysXaa: 0.0 ± 0.0
Leu
6.388LeuAla: 6.388 ± 0.066
0.772LeuCys: 0.772 ± 0.027
4.539LeuAsp: 4.539 ± 0.057
6.168LeuGlu: 6.168 ± 0.087
5.148LeuPhe: 5.148 ± 0.085
6.283LeuGly: 6.283 ± 0.089
1.8LeuHis: 1.8 ± 0.033
8.13LeuIle: 8.13 ± 0.11
7.266LeuLys: 7.266 ± 0.075
9.982LeuLeu: 9.982 ± 0.137
2.372LeuMet: 2.372 ± 0.048
5.307LeuAsn: 5.307 ± 0.064
3.838LeuPro: 3.838 ± 0.064
3.402LeuGln: 3.402 ± 0.057
3.171LeuArg: 3.171 ± 0.055
7.12LeuSer: 7.12 ± 0.075
5.571LeuThr: 5.571 ± 0.067
6.452LeuVal: 6.452 ± 0.077
0.763LeuTrp: 0.763 ± 0.026
3.143LeuTyr: 3.143 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
1.595MetAla: 1.595 ± 0.037
0.137MetCys: 0.137 ± 0.011
1.205MetAsp: 1.205 ± 0.031
1.671MetGlu: 1.671 ± 0.04
0.995MetPhe: 0.995 ± 0.027
1.521MetGly: 1.521 ± 0.037
0.417MetHis: 0.417 ± 0.02
2.228MetIle: 2.228 ± 0.043
2.439MetLys: 2.439 ± 0.041
2.264MetLeu: 2.264 ± 0.04
0.755MetMet: 0.755 ± 0.027
1.746MetAsn: 1.746 ± 0.034
0.897MetPro: 0.897 ± 0.03
0.745MetGln: 0.745 ± 0.024
0.902MetArg: 0.902 ± 0.03
1.69MetSer: 1.69 ± 0.033
1.44MetThr: 1.44 ± 0.033
1.575MetVal: 1.575 ± 0.037
0.143MetTrp: 0.143 ± 0.011
0.759MetTyr: 0.759 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.022AsnAla: 3.022 ± 0.054
0.407AsnCys: 0.407 ± 0.018
2.76AsnAsp: 2.76 ± 0.049
4.315AsnGlu: 4.315 ± 0.075
2.264AsnPhe: 2.264 ± 0.043
3.897AsnGly: 3.897 ± 0.073
1.198AsnHis: 1.198 ± 0.031
4.26AsnIle: 4.26 ± 0.066
4.07AsnLys: 4.07 ± 0.058
4.769AsnLeu: 4.769 ± 0.062
1.27AsnMet: 1.27 ± 0.034
2.887AsnAsn: 2.887 ± 0.052
2.189AsnPro: 2.189 ± 0.04
2.18AsnGln: 2.18 ± 0.045
2.036AsnArg: 2.036 ± 0.047
3.24AsnSer: 3.24 ± 0.051
2.553AsnThr: 2.553 ± 0.047
3.47AsnVal: 3.47 ± 0.064
0.606AsnTrp: 0.606 ± 0.021
2.064AsnTyr: 2.064 ± 0.036
0.0AsnXaa: 0.0 ± 0.0
Pro
1.951ProAla: 1.951 ± 0.059
0.19ProCys: 0.19 ± 0.012
1.724ProAsp: 1.724 ± 0.035
2.36ProGlu: 2.36 ± 0.048
2.072ProPhe: 2.072 ± 0.041
2.111ProGly: 2.111 ± 0.045
0.699ProHis: 0.699 ± 0.023
3.212ProIle: 3.212 ± 0.048
2.349ProLys: 2.349 ± 0.041
3.211ProLeu: 3.211 ± 0.054
0.765ProMet: 0.765 ± 0.024
2.023ProAsn: 2.023 ± 0.053
0.851ProPro: 0.851 ± 0.03
0.907ProGln: 0.907 ± 0.025
0.906ProArg: 0.906 ± 0.027
2.357ProSer: 2.357 ± 0.049
2.234ProThr: 2.234 ± 0.042
2.581ProVal: 2.581 ± 0.044
0.347ProTrp: 0.347 ± 0.02
1.445ProTyr: 1.445 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
2.152GlnAla: 2.152 ± 0.043
0.182GlnCys: 0.182 ± 0.014
1.477GlnAsp: 1.477 ± 0.032
2.288GlnGlu: 2.288 ± 0.046
1.725GlnPhe: 1.725 ± 0.038
1.824GlnGly: 1.824 ± 0.04
0.644GlnHis: 0.644 ± 0.023
2.941GlnIle: 2.941 ± 0.043
2.765GlnLys: 2.765 ± 0.05
3.642GlnLeu: 3.642 ± 0.066
0.939GlnMet: 0.939 ± 0.027
1.913GlnAsn: 1.913 ± 0.043
1.022GlnPro: 1.022 ± 0.03
1.379GlnGln: 1.379 ± 0.04
1.218GlnArg: 1.218 ± 0.031
2.24GlnSer: 2.24 ± 0.039
1.904GlnThr: 1.904 ± 0.039
2.242GlnVal: 2.242 ± 0.048
0.338GlnTrp: 0.338 ± 0.018
1.383GlnTyr: 1.383 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
1.987ArgAla: 1.987 ± 0.044
0.246ArgCys: 0.246 ± 0.016
1.771ArgAsp: 1.771 ± 0.039
2.599ArgGlu: 2.599 ± 0.055
1.764ArgPhe: 1.764 ± 0.037
2.052ArgGly: 2.052 ± 0.044
0.647ArgHis: 0.647 ± 0.023
2.968ArgIle: 2.968 ± 0.055
2.957ArgLys: 2.957 ± 0.047
3.397ArgLeu: 3.397 ± 0.06
1.029ArgMet: 1.029 ± 0.032
1.988ArgAsn: 1.988 ± 0.035
1.085ArgPro: 1.085 ± 0.026
1.16ArgGln: 1.16 ± 0.032
1.432ArgArg: 1.432 ± 0.04
2.026ArgSer: 2.026 ± 0.041
1.858ArgThr: 1.858 ± 0.044
2.306ArgVal: 2.306 ± 0.044
0.326ArgTrp: 0.326 ± 0.017
1.378ArgTyr: 1.378 ± 0.038
0.001ArgXaa: 0.001 ± 0.001
Ser
3.548SerAla: 3.548 ± 0.061
0.424SerCys: 0.424 ± 0.018
2.935SerAsp: 2.935 ± 0.052
3.869SerGlu: 3.869 ± 0.054
3.676SerPhe: 3.676 ± 0.053
4.345SerGly: 4.345 ± 0.063
1.169SerHis: 1.169 ± 0.03
6.213SerIle: 6.213 ± 0.074
4.992SerLys: 4.992 ± 0.066
6.479SerLeu: 6.479 ± 0.086
1.658SerMet: 1.658 ± 0.035
3.617SerAsn: 3.617 ± 0.057
2.162SerPro: 2.162 ± 0.043
1.903SerGln: 1.903 ± 0.037
2.11SerArg: 2.11 ± 0.041
4.426SerSer: 4.426 ± 0.068
3.732SerThr: 3.732 ± 0.068
4.301SerVal: 4.301 ± 0.059
0.618SerTrp: 0.618 ± 0.022
2.495SerTyr: 2.495 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
3.523ThrAla: 3.523 ± 0.064
0.365ThrCys: 0.365 ± 0.018
2.561ThrAsp: 2.561 ± 0.038
3.25ThrGlu: 3.25 ± 0.049
2.896ThrPhe: 2.896 ± 0.048
4.076ThrGly: 4.076 ± 0.096
1.051ThrHis: 1.051 ± 0.03
5.279ThrIle: 5.279 ± 0.073
3.872ThrLys: 3.872 ± 0.061
5.376ThrLeu: 5.376 ± 0.074
1.234ThrMet: 1.234 ± 0.029
3.119ThrAsn: 3.119 ± 0.054
2.254ThrPro: 2.254 ± 0.043
1.422ThrGln: 1.422 ± 0.033
1.623ThrArg: 1.623 ± 0.042
3.662ThrSer: 3.662 ± 0.061
3.167ThrThr: 3.167 ± 0.065
4.133ThrVal: 4.133 ± 0.062
0.483ThrTrp: 0.483 ± 0.021
2.056ThrTyr: 2.056 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
4.52ValAla: 4.52 ± 0.066
0.59ValCys: 0.59 ± 0.022
3.504ValAsp: 3.504 ± 0.069
4.57ValGlu: 4.57 ± 0.074
3.202ValPhe: 3.202 ± 0.053
4.77ValGly: 4.77 ± 0.075
1.268ValHis: 1.268 ± 0.03
6.005ValIle: 6.005 ± 0.078
5.025ValLys: 5.025 ± 0.072
6.526ValLeu: 6.526 ± 0.083
1.592ValMet: 1.592 ± 0.037
3.545ValAsn: 3.545 ± 0.052
2.544ValPro: 2.544 ± 0.047
2.187ValGln: 2.187 ± 0.041
2.259ValArg: 2.259 ± 0.05
4.771ValSer: 4.771 ± 0.068
4.109ValThr: 4.109 ± 0.068
5.034ValVal: 5.034 ± 0.078
0.615ValTrp: 0.615 ± 0.023
2.294ValTyr: 2.294 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.505TrpAla: 0.505 ± 0.023
0.075TrpCys: 0.075 ± 0.008
0.507TrpAsp: 0.507 ± 0.019
0.57TrpGlu: 0.57 ± 0.022
0.515TrpPhe: 0.515 ± 0.023
0.618TrpGly: 0.618 ± 0.025
0.187TrpHis: 0.187 ± 0.013
0.863TrpIle: 0.863 ± 0.027
0.791TrpLys: 0.791 ± 0.028
0.999TrpLeu: 0.999 ± 0.029
0.278TrpMet: 0.278 ± 0.013
0.599TrpAsn: 0.599 ± 0.022
0.23TrpPro: 0.23 ± 0.015
0.298TrpGln: 0.298 ± 0.014
0.346TrpArg: 0.346 ± 0.019
0.619TrpSer: 0.619 ± 0.027
0.485TrpThr: 0.485 ± 0.02
0.586TrpVal: 0.586 ± 0.019
0.12TrpTrp: 0.12 ± 0.009
0.379TrpTyr: 0.379 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.998TyrAla: 1.998 ± 0.041
0.319TyrCys: 0.319 ± 0.015
1.957TyrAsp: 1.957 ± 0.049
2.555TyrGlu: 2.555 ± 0.05
2.088TyrPhe: 2.088 ± 0.044
2.482TyrGly: 2.482 ± 0.05
0.795TyrHis: 0.795 ± 0.027
2.899TyrIle: 2.899 ± 0.05
2.652TyrLys: 2.652 ± 0.043
3.782TyrLeu: 3.782 ± 0.058
0.813TyrMet: 0.813 ± 0.025
1.825TyrAsn: 1.825 ± 0.038
1.341TyrPro: 1.341 ± 0.034
1.349TyrGln: 1.349 ± 0.034
1.413TyrArg: 1.413 ± 0.035
2.492TyrSer: 2.492 ± 0.05
1.965TyrThr: 1.965 ± 0.044
2.24TyrVal: 2.24 ± 0.038
0.414TyrTrp: 0.414 ± 0.02
1.578TyrTyr: 1.578 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.001XaaCys: 0.001 ± 0.001
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.009XaaXaa: 0.009 ± 0.009
Statistics based on 4458 proteins (1318057 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski