Lachancea fermentati (Zygosaccharomyces fermentati)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota;

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G4M9J3|A0A1G4M9J3_LACFM LAFE_0C06260g1_1 OS=Lachancea fermentati OX=4955 GN=LAFE_0C06260G PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 3.27SIEE6 pKa = 4.41YY7 pKa = 8.28PTEE10 pKa = 4.38DD11 pKa = 3.71DD12 pKa = 3.85VFSFSLANQPTAQWSAQWNGLGSTVTPPLLSNTPDD47 pKa = 3.25SYY49 pKa = 10.38THH51 pKa = 6.68EE52 pKa = 4.12EE53 pKa = 4.34HH54 pKa = 7.17EE55 pKa = 4.72DD56 pKa = 3.45EE57 pKa = 5.15FLISSPLMYY66 pKa = 10.42AVQSNGRR73 pKa = 11.84LDD75 pKa = 5.03DD76 pKa = 4.24DD77 pKa = 5.38LFRR80 pKa = 11.84LDD82 pKa = 4.67QDD84 pKa = 3.71EE85 pKa = 4.58LACGVANDD93 pKa = 4.5ASFAACSATPRR104 pKa = 11.84VLVEE108 pKa = 3.95NSEE111 pKa = 4.5SEE113 pKa = 4.42LFANAAQEE121 pKa = 4.29NYY123 pKa = 10.0RR124 pKa = 11.84LWLTGVV130 pKa = 3.31

Molecular weight: 14.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G4M841|A0A1G4M841_LACFM LAFE_0B07712g1_1 OS=Lachancea fermentati OX=4955 GN=LAFE_0B07712G PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.11WRR6 pKa = 11.84KK7 pKa = 9.07KK8 pKa = 6.93RR9 pKa = 11.84TRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.48RR15 pKa = 11.84KK16 pKa = 8.41RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5229

0

5229

2551543

25

4920

488.0

54.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.243 ± 0.034

1.345 ± 0.013

5.833 ± 0.022

6.738 ± 0.032

4.432 ± 0.021

5.375 ± 0.026

2.21 ± 0.014

6.027 ± 0.023

6.742 ± 0.032

9.744 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.082 ± 0.011

5.023 ± 0.023

4.452 ± 0.025

4.03 ± 0.024

4.857 ± 0.023

8.818 ± 0.043

5.59 ± 0.035

6.088 ± 0.021

1.094 ± 0.01

3.279 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski