Changjiang hepe-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJL8|A0A1L3KJL8_9VIRU Uncharacterized protein OS=Changjiang hepe-like virus 1 OX=1922772 PE=4 SV=1
MM1 pKa = 7.6TNFQPRR7 pKa = 11.84AHH9 pKa = 6.11TLEE12 pKa = 3.91VLQTNSEE19 pKa = 4.22HH20 pKa = 6.48TNQQFDD26 pKa = 3.56VSNANLQRR34 pKa = 11.84VLDD37 pKa = 3.63QFIINNEE44 pKa = 3.98KK45 pKa = 10.5LSQLNQQLATALEE58 pKa = 4.13NQTIIINKK66 pKa = 7.47FTEE69 pKa = 4.07QLLKK73 pKa = 10.03TDD75 pKa = 4.77SLIQNQQSSIEE86 pKa = 4.02HH87 pKa = 6.04QYY89 pKa = 11.24NIQKK93 pKa = 10.39SLEE96 pKa = 4.04AVQQTVTQTNGDD108 pKa = 3.64VSRR111 pKa = 11.84IATTVRR117 pKa = 11.84LGGKK121 pKa = 9.07NALAVTNTAADD132 pKa = 4.8PIWTIPAGG140 pKa = 3.55

Molecular weight: 15.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJN1|A0A1L3KJN1_9VIRU Non-structural protein 2 OS=Changjiang hepe-like virus 1 OX=1922772 PE=4 SV=1
MM1 pKa = 7.21PQTRR5 pKa = 11.84SEE7 pKa = 4.09HH8 pKa = 6.19SSNITWTPSSGQKK21 pKa = 9.1PASPSSHH28 pKa = 5.45QAPVLRR34 pKa = 11.84VGGARR39 pKa = 11.84ISRR42 pKa = 11.84VKK44 pKa = 9.78STASSRR50 pKa = 11.84TCSICAAHH58 pKa = 6.55CRR60 pKa = 11.84KK61 pKa = 10.17SLTNSKK67 pKa = 9.03HH68 pKa = 4.74HH69 pKa = 6.94HH70 pKa = 6.18EE71 pKa = 4.74NSLTLNDD78 pKa = 4.41RR79 pKa = 11.84SPPSNSRR86 pKa = 11.84RR87 pKa = 11.84LIFCDD92 pKa = 5.77APNCWLFVFHH102 pKa = 6.49SLCRR106 pKa = 11.84ACMMFPTQTVRR117 pKa = 11.84DD118 pKa = 3.66RR119 pKa = 11.84FRR121 pKa = 11.84RR122 pKa = 11.84LLAARR127 pKa = 11.84QLHH130 pKa = 6.31KK131 pKa = 10.45ISYY134 pKa = 7.95CCCARR139 pKa = 11.84NQNSFRR145 pKa = 11.84QIGPHH150 pKa = 6.88RR151 pKa = 11.84YY152 pKa = 7.8TNIAIQNPISFINHH166 pKa = 4.08VVDD169 pKa = 3.93YY170 pKa = 10.0FLL172 pKa = 6.21

Molecular weight: 19.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3340

140

2290

668.0

74.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.491 ± 0.626

1.527 ± 0.598

6.078 ± 0.649

4.371 ± 0.619

3.922 ± 0.343

5.449 ± 0.502

3.353 ± 0.432

6.168 ± 0.178

4.79 ± 0.851

8.473 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.617 ± 0.224

5.329 ± 0.631

4.491 ± 0.397

4.91 ± 0.942

5.898 ± 0.689

6.048 ± 1.78

7.066 ± 0.632

6.856 ± 0.531

0.988 ± 0.145

3.174 ± 0.401
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski