Sphingomonadaceae bacterium PASS1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; unclassified Sphingomonadaceae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2834 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257DNB8|A0A257DNB8_9SPHN Acyl-CoA dehydrogenase OS=Sphingomonadaceae bacterium PASS1 OX=2015579 GN=CFE36_01800 PE=3 SV=1
MM1 pKa = 7.6HH2 pKa = 6.7EE3 pKa = 3.84TWEE6 pKa = 4.02QAEE9 pKa = 4.06YY10 pKa = 10.39RR11 pKa = 11.84QYY13 pKa = 10.98SCLFCGHH20 pKa = 7.22IYY22 pKa = 10.63DD23 pKa = 4.57EE24 pKa = 4.9EE25 pKa = 5.07EE26 pKa = 4.34GDD28 pKa = 3.99SDD30 pKa = 4.91SGIAPGTRR38 pKa = 11.84WEE40 pKa = 5.39DD41 pKa = 4.29ISDD44 pKa = 3.69DD45 pKa = 4.03WCCPMCSAGKK55 pKa = 10.44ADD57 pKa = 4.48FEE59 pKa = 5.59LII61 pKa = 3.86

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257DNJ1|A0A257DNJ1_9SPHN Imidazole glycerol phosphate synthase subunit HisF OS=Sphingomonadaceae bacterium PASS1 OX=2015579 GN=hisF PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTAAGRR28 pKa = 11.84NILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2834

0

2834

930857

32

4232

328.5

35.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.178 ± 0.055

0.863 ± 0.016

5.997 ± 0.037

5.311 ± 0.045

3.844 ± 0.03

8.596 ± 0.045

2.067 ± 0.025

5.707 ± 0.03

3.867 ± 0.036

9.353 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.717 ± 0.022

3.242 ± 0.036

4.912 ± 0.025

3.253 ± 0.023

6.125 ± 0.043

5.735 ± 0.036

5.342 ± 0.04

7.024 ± 0.034

1.431 ± 0.018

2.437 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski