Cyanophage Syn2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Pontusvirus; unclassified Pontusvirus

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4SJH5|M4SJH5_9CAUD Uncharacterized protein OS=Cyanophage Syn2 OX=536473 GN=CPTG_00191 PE=4 SV=1
MM1 pKa = 7.85FDD3 pKa = 3.11TTLDD7 pKa = 4.04LFCHH11 pKa = 6.47NDD13 pKa = 3.15NLNDD17 pKa = 4.04RR18 pKa = 11.84DD19 pKa = 3.95HH20 pKa = 7.14MIDD23 pKa = 4.92QMAQLYY29 pKa = 8.69FRR31 pKa = 11.84AMTTNANKK39 pKa = 10.7GEE41 pKa = 4.12NFDD44 pKa = 5.86AIACYY49 pKa = 9.72EE50 pKa = 4.09EE51 pKa = 4.53WVVDD55 pKa = 4.35GVDD58 pKa = 3.81PQDD61 pKa = 2.99VDD63 pKa = 3.83YY64 pKa = 11.22EE65 pKa = 4.76FIFAPDD71 pKa = 3.22LTEE74 pKa = 3.87EE75 pKa = 4.24RR76 pKa = 11.84EE77 pKa = 4.26EE78 pKa = 4.09NN79 pKa = 3.44

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4SK42|M4SK42_9CAUD Uncharacterized protein OS=Cyanophage Syn2 OX=536473 GN=CPTG_00060 PE=4 SV=1
MM1 pKa = 7.46TSQRR5 pKa = 11.84CRR7 pKa = 11.84TIRR10 pKa = 11.84KK11 pKa = 8.52AWRR14 pKa = 11.84VWAKK18 pKa = 11.09ALGQKK23 pKa = 10.19DD24 pKa = 3.43GRR26 pKa = 11.84NDD28 pKa = 3.5RR29 pKa = 11.84EE30 pKa = 3.69ADD32 pKa = 3.72TIAGIRR38 pKa = 11.84TLIFVSYY45 pKa = 9.24MVTNIAIVANAVRR58 pKa = 11.84HH59 pKa = 5.44WDD61 pKa = 3.66SNQSVPVVSPHH72 pKa = 7.07LDD74 pKa = 3.24DD75 pKa = 5.2SAILNKK81 pKa = 10.4

Molecular weight: 9.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

201

0

201

55131

46

3866

274.3

30.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.212 ± 0.263

0.767 ± 0.087

6.63 ± 0.136

5.998 ± 0.253

4.435 ± 0.092

8.211 ± 0.375

1.498 ± 0.126

6.056 ± 0.195

5.806 ± 0.353

7.241 ± 0.163

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.111 ± 0.196

5.752 ± 0.171

3.871 ± 0.176

3.796 ± 0.097

4.17 ± 0.161

7.021 ± 0.21

7.533 ± 0.464

6.642 ± 0.186

1.134 ± 0.093

4.114 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski