Alternaria longipes dsRNA virus 1

Taxonomy: Viruses; Riboviria; dsRNA viruses; unclassified dsRNA viruses

Average proteome isoelectric point is 7.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076L1I6|A0A076L1I6_9VIRU Uncharacterized protein OS=Alternaria longipes dsRNA virus 1 OX=1532031 PE=4 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.26NPSTTSDD10 pKa = 3.22AAGKK14 pKa = 9.84RR15 pKa = 11.84RR16 pKa = 11.84PPTEE20 pKa = 2.78WKK22 pKa = 9.43MFLEE26 pKa = 4.6NRR28 pKa = 11.84SHH30 pKa = 7.06RR31 pKa = 11.84PVPGVKK37 pKa = 10.3YY38 pKa = 9.31MGSSSHH44 pKa = 6.51LRR46 pKa = 11.84YY47 pKa = 9.99KK48 pKa = 10.39YY49 pKa = 9.79VQPEE53 pKa = 4.38TNTSSFFCKK62 pKa = 10.3FLDD65 pKa = 3.38QSTVVGGEE73 pKa = 3.79IDD75 pKa = 3.88PPSADD80 pKa = 2.79VVMSEE85 pKa = 4.18FVPPAEE91 pKa = 4.02EE92 pKa = 4.65DD93 pKa = 3.09LWKK96 pKa = 10.56HH97 pKa = 5.45VEE99 pKa = 4.69GFGQNQNRR107 pKa = 11.84GGGPSQASVSDD118 pKa = 3.18ILNEE122 pKa = 3.76LRR124 pKa = 11.84RR125 pKa = 11.84LEE127 pKa = 4.08GARR130 pKa = 11.84PIIDD134 pKa = 3.49DD135 pKa = 3.61WFEE138 pKa = 3.98ASALPKK144 pKa = 10.06IRR146 pKa = 11.84VPAHH150 pKa = 5.75TSPGIRR156 pKa = 11.84WKK158 pKa = 10.87KK159 pKa = 10.21LGYY162 pKa = 7.89KK163 pKa = 8.13TKK165 pKa = 10.46RR166 pKa = 11.84DD167 pKa = 3.65ALMPAIIEE175 pKa = 3.99ATRR178 pKa = 11.84IIGKK182 pKa = 9.67LKK184 pKa = 9.38EE185 pKa = 4.23TGHH188 pKa = 6.52EE189 pKa = 4.04YY190 pKa = 10.64DD191 pKa = 3.83VPPAGVAGRR200 pKa = 11.84GKK202 pKa = 10.08RR203 pKa = 11.84VALDD207 pKa = 3.75RR208 pKa = 11.84PDD210 pKa = 4.46DD211 pKa = 4.23PDD213 pKa = 3.6RR214 pKa = 11.84KK215 pKa = 9.19EE216 pKa = 3.66GRR218 pKa = 11.84LIVMPDD224 pKa = 3.6LVRR227 pKa = 11.84HH228 pKa = 6.16LLGAMAAAPYY238 pKa = 10.31MSMQRR243 pKa = 11.84SLDD246 pKa = 3.48KK247 pKa = 11.3SRR249 pKa = 11.84GGVMLGMGPFQEE261 pKa = 4.78SYY263 pKa = 11.2QDD265 pKa = 3.29IAGWAAGAKK274 pKa = 9.35RR275 pKa = 11.84YY276 pKa = 10.2VFIDD280 pKa = 3.94FKK282 pKa = 11.32KK283 pKa = 9.46FDD285 pKa = 3.3QRR287 pKa = 11.84IPRR290 pKa = 11.84RR291 pKa = 11.84VLRR294 pKa = 11.84AVMKK298 pKa = 10.03HH299 pKa = 5.58ISHH302 pKa = 6.84SYY304 pKa = 10.09SNGPGTGAYY313 pKa = 6.78WASEE317 pKa = 3.71FRR319 pKa = 11.84HH320 pKa = 5.63LVEE323 pKa = 5.17TEE325 pKa = 3.23IAMPNGSVYY334 pKa = 10.76KK335 pKa = 10.46KK336 pKa = 10.71FMGVASGDD344 pKa = 3.51PWTSLADD351 pKa = 3.86SYY353 pKa = 11.86ANWVMLSMACKK364 pKa = 10.33ALGLRR369 pKa = 11.84AKK371 pKa = 9.79IWTFGDD377 pKa = 3.82DD378 pKa = 3.21SVLAIDD384 pKa = 4.54EE385 pKa = 4.54GDD387 pKa = 3.4TRR389 pKa = 11.84GDD391 pKa = 3.33LLGRR395 pKa = 11.84ISEE398 pKa = 4.35WVSKK402 pKa = 10.05EE403 pKa = 3.42FGMVVSKK410 pKa = 10.49EE411 pKa = 3.92KK412 pKa = 10.94SYY414 pKa = 10.62MATDD418 pKa = 4.29LVTIDD423 pKa = 4.66EE424 pKa = 4.99DD425 pKa = 3.9PQEE428 pKa = 4.29GLSGSFLSMYY438 pKa = 10.64FLATPMGVRR447 pKa = 11.84PTRR450 pKa = 11.84PLQHH454 pKa = 6.8FYY456 pKa = 11.42EE457 pKa = 4.81LFLKK461 pKa = 10.26PEE463 pKa = 4.33RR464 pKa = 11.84NGGTVEE470 pKa = 3.95WEE472 pKa = 4.13VARR475 pKa = 11.84TSMAYY480 pKa = 10.32LVFYY484 pKa = 8.71YY485 pKa = 9.86NWKK488 pKa = 9.18VRR490 pKa = 11.84YY491 pKa = 9.0ILHH494 pKa = 6.87EE495 pKa = 3.98YY496 pKa = 9.01WDD498 pKa = 4.09WLHH501 pKa = 6.37EE502 pKa = 4.31RR503 pKa = 11.84YY504 pKa = 9.89KK505 pKa = 10.55IPEE508 pKa = 4.0LRR510 pKa = 11.84GTLDD514 pKa = 3.35DD515 pKa = 4.73LRR517 pKa = 11.84LLRR520 pKa = 11.84EE521 pKa = 4.01LDD523 pKa = 3.5IPWAHH528 pKa = 6.78FKK530 pKa = 11.03LSWLSHH536 pKa = 6.5LPPAGEE542 pKa = 4.03VEE544 pKa = 4.22LLYY547 pKa = 10.68KK548 pKa = 10.57YY549 pKa = 10.83GHH551 pKa = 5.65TCFYY555 pKa = 10.85PPVLWDD561 pKa = 3.43KK562 pKa = 11.03LYY564 pKa = 11.21SRR566 pKa = 11.84NSEE569 pKa = 4.1LPGGNSFADD578 pKa = 3.42WW579 pKa = 3.5

Molecular weight: 65.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076L1I6|A0A076L1I6_9VIRU Uncharacterized protein OS=Alternaria longipes dsRNA virus 1 OX=1532031 PE=4 SV=1
MM1 pKa = 6.97VVAEE5 pKa = 4.67ALRR8 pKa = 11.84MSLEE12 pKa = 4.29RR13 pKa = 11.84SWSSTLQWIRR23 pKa = 11.84VGTAWEE29 pKa = 3.98PRR31 pKa = 11.84PPVDD35 pKa = 4.9KK36 pKa = 11.34SMEE39 pKa = 4.4TTGPPPSGTGAPGSQTPPAGQGSARR64 pKa = 11.84PTQHH68 pKa = 6.15EE69 pKa = 4.3RR70 pKa = 11.84SASQPAANRR79 pKa = 11.84EE80 pKa = 3.92ARR82 pKa = 11.84TPPARR87 pKa = 11.84PAEE90 pKa = 4.1YY91 pKa = 10.06TEE93 pKa = 4.0LAGAFEE99 pKa = 4.41RR100 pKa = 11.84RR101 pKa = 11.84PRR103 pKa = 11.84PQTPIQPIRR112 pKa = 11.84GLNVTSPEE120 pKa = 3.73RR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84SGGSSMPPPPPPKK136 pKa = 9.75KK137 pKa = 10.43DD138 pKa = 2.96GGTAKK143 pKa = 10.2SAPPADD149 pKa = 4.0PDD151 pKa = 3.05AWALRR156 pKa = 11.84IYY158 pKa = 9.96TIVRR162 pKa = 11.84QWHH165 pKa = 6.77DD166 pKa = 3.76LNGVTGVVTDD176 pKa = 4.34PRR178 pKa = 11.84SLFPGDD184 pKa = 2.94WTLGRR189 pKa = 11.84TRR191 pKa = 11.84LVYY194 pKa = 10.68DD195 pKa = 3.71PGVAGRR201 pKa = 11.84YY202 pKa = 7.39LRR204 pKa = 11.84RR205 pKa = 11.84MLPDD209 pKa = 3.64LKK211 pKa = 10.69CYY213 pKa = 8.11TSKK216 pKa = 10.86GVEE219 pKa = 4.02TAGVVQGSDD228 pKa = 3.26DD229 pKa = 3.74VKK231 pKa = 9.78TARR234 pKa = 11.84ITAEE238 pKa = 3.37WRR240 pKa = 11.84PPVHH244 pKa = 6.58LVAEE248 pKa = 4.12ILSYY252 pKa = 10.61KK253 pKa = 9.09SEE255 pKa = 4.13RR256 pKa = 11.84TVSADD261 pKa = 3.28EE262 pKa = 4.38VVALAQSAPDD272 pKa = 3.46ALEE275 pKa = 3.93KK276 pKa = 10.82LKK278 pKa = 10.88EE279 pKa = 4.08AGKK282 pKa = 9.85SLKK285 pKa = 10.65AHH287 pKa = 6.21FTRR290 pKa = 11.84LLDD293 pKa = 3.51MVALRR298 pKa = 11.84RR299 pKa = 11.84SPAYY303 pKa = 10.31NEE305 pKa = 3.31TAARR309 pKa = 11.84YY310 pKa = 7.84KK311 pKa = 10.81AQLKK315 pKa = 10.21VLIQEE320 pKa = 4.36QYY322 pKa = 10.51NHH324 pKa = 5.84VLALKK329 pKa = 10.21QATKK333 pKa = 10.53RR334 pKa = 11.84VNDD337 pKa = 4.04LLAEE341 pKa = 4.5RR342 pKa = 11.84DD343 pKa = 3.69AEE345 pKa = 4.63LARR348 pKa = 11.84LDD350 pKa = 4.1PGYY353 pKa = 9.26TPKK356 pKa = 10.6KK357 pKa = 10.33QSAQDD362 pKa = 3.18ALARR366 pKa = 11.84YY367 pKa = 9.16GIDD370 pKa = 3.28LGSEE374 pKa = 4.07GGEE377 pKa = 4.11EE378 pKa = 4.16SWEE381 pKa = 4.01EE382 pKa = 3.91ANPDD386 pKa = 3.51NPLPNLDD393 pKa = 3.75FF394 pKa = 4.95

Molecular weight: 43.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

973

394

579

486.5

54.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.941 ± 1.513

0.411 ± 0.096

5.755 ± 0.26

6.475 ± 0.076

2.775 ± 1.074

7.811 ± 0.43

2.055 ± 0.48

3.289 ± 0.613

5.242 ± 0.411

8.736 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.775 ± 0.764

2.261 ± 0.014

8.222 ± 1.332

2.775 ± 0.785

7.708 ± 0.717

7.194 ± 0.208

5.036 ± 0.954

6.166 ± 0.046

2.672 ± 0.391

3.7 ± 0.554
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski