Gammapapillomavirus 8
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2ALG0|A0A2D2ALG0_9PAPI Minor capsid protein L2 OS=Gammapapillomavirus 8 OX=1175850 GN=L2 PE=3 SV=1
MM1 pKa = 6.42 YY2 pKa = 7.79 TAKK5 pKa = 10.2 RR6 pKa = 11.84 VKK8 pKa = 9.97 RR9 pKa = 11.84 DD10 pKa = 3.33 SPEE13 pKa = 3.67 NIYY16 pKa = 10.12 RR17 pKa = 11.84 HH18 pKa = 5.92 CKK20 pKa = 9.1 IAGTCPPDD28 pKa = 3.44 VEE30 pKa = 5.18 NKK32 pKa = 9.9 IEE34 pKa = 4.0 QNTLADD40 pKa = 4.01 RR41 pKa = 11.84 LLKK44 pKa = 10.46 IFGSIIYY51 pKa = 10.28 LGGLGIGSGRR61 pKa = 11.84 GTGAATGFRR70 pKa = 11.84 PLPDD74 pKa = 3.99 EE75 pKa = 4.84 VPLPDD80 pKa = 3.81 TNIITDD86 pKa = 3.51 SSTLPEE92 pKa = 4.06 TVRR95 pKa = 11.84 PSLRR99 pKa = 11.84 PRR101 pKa = 11.84 PSRR104 pKa = 11.84 PNTFGVPLDD113 pKa = 5.03 PISSADD119 pKa = 3.6 NIPRR123 pKa = 11.84 IVRR126 pKa = 11.84 PTEE129 pKa = 3.59 PAIVPLSEE137 pKa = 5.1 GGLPDD142 pKa = 3.46 PTVISTGVEE151 pKa = 3.83 SGAGVGDD158 pKa = 3.85 YY159 pKa = 10.67 EE160 pKa = 4.38 VLTNIFVDD168 pKa = 3.97 DD169 pKa = 3.89 ALGAVGGHH177 pKa = 5.46 PTVTSGVNEE186 pKa = 4.29 NIALLEE192 pKa = 3.99 ITPVEE197 pKa = 4.69 HH198 pKa = 6.05 IPSRR202 pKa = 11.84 ITYY205 pKa = 6.96 TTTDD209 pKa = 2.74 VDD211 pKa = 3.23 TGYY214 pKa = 8.87 TFIRR218 pKa = 11.84 SSLPADD224 pKa = 3.65 PDD226 pKa = 3.55 MNVFVDD232 pKa = 3.74 PRR234 pKa = 11.84 YY235 pKa = 10.16 SGTVVGDD242 pKa = 3.86 NIEE245 pKa = 4.4 LEE247 pKa = 4.65 AIHH250 pKa = 6.79 NIDD253 pKa = 3.09 QFEE256 pKa = 4.21 IEE258 pKa = 4.65 EE259 pKa = 4.35 AGQPQTSTPSRR270 pKa = 11.84 ISDD273 pKa = 3.51 WAKK276 pKa = 10.41 LRR278 pKa = 11.84 AQEE281 pKa = 4.25 FYY283 pKa = 11.49 NRR285 pKa = 11.84 FIQQMPTRR293 pKa = 11.84 NIDD296 pKa = 3.63 FLGQASRR303 pKa = 11.84 AVQFEE308 pKa = 4.18 FEE310 pKa = 4.24 NPAFEE315 pKa = 5.03 SDD317 pKa = 3.13 VSLQFEE323 pKa = 4.09 RR324 pKa = 11.84 DD325 pKa = 3.77 VQEE328 pKa = 4.14 LAAAPDD334 pKa = 3.52 EE335 pKa = 4.44 AFTDD339 pKa = 3.89 IVRR342 pKa = 11.84 LGRR345 pKa = 11.84 PVLSEE350 pKa = 4.12 TPAGNVRR357 pKa = 11.84 VSRR360 pKa = 11.84 LGTRR364 pKa = 11.84 GGMRR368 pKa = 11.84 TRR370 pKa = 11.84 SGTILKK376 pKa = 10.2 QKK378 pKa = 9.57 VHH380 pKa = 6.54 FFYY383 pKa = 11.0 DD384 pKa = 3.92 LSPIAAVDD392 pKa = 3.68 EE393 pKa = 4.55 SAATNIEE400 pKa = 3.84 LANFGEE406 pKa = 4.52 SSDD409 pKa = 3.23 ILTIVDD415 pKa = 3.96 EE416 pKa = 4.62 LSGGRR421 pKa = 11.84 PINAFEE427 pKa = 4.79 EE428 pKa = 4.34 YY429 pKa = 10.76 DD430 pKa = 3.56 EE431 pKa = 4.43 TALLNTDD438 pKa = 3.16 EE439 pKa = 4.97 ATFQNAHH446 pKa = 6.41 LEE448 pKa = 4.38 VYY450 pKa = 10.06 TDD452 pKa = 4.64 DD453 pKa = 5.28 LEE455 pKa = 4.51 EE456 pKa = 4.16 PQIIPSLITDD466 pKa = 3.8 VTPKK470 pKa = 10.77 NFVNPYY476 pKa = 7.64 ITDD479 pKa = 3.41 ILVSYY484 pKa = 9.47 PSIIDD489 pKa = 3.58 NFPFTPIVPTTPLSPTVQIDD509 pKa = 3.58 AFGSDD514 pKa = 4.44 YY515 pKa = 11.04 YY516 pKa = 11.08 LHH518 pKa = 7.28 PSLLRR523 pKa = 11.84 RR524 pKa = 11.84 KK525 pKa = 8.48 KK526 pKa = 10.46 RR527 pKa = 11.84 KK528 pKa = 7.58 WFEE531 pKa = 3.63 MSS533 pKa = 2.85
Molecular weight: 58.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.593
IPC2_protein 4.52
IPC_protein 4.482
Toseland 4.317
ProMoST 4.596
Dawson 4.444
Bjellqvist 4.584
Wikipedia 4.317
Rodwell 4.317
Grimsley 4.228
Solomon 4.431
Lehninger 4.393
Nozaki 4.546
DTASelect 4.724
Thurlkill 4.317
EMBOSS 4.329
Sillero 4.596
Patrickios 3.961
IPC_peptide 4.444
IPC2_peptide 4.584
IPC2.peptide.svr19 4.552
Protein with the highest isoelectric point:
>tr|A0A2D2ALE6|A0A2D2ALE6_9PAPI Protein E6 OS=Gammapapillomavirus 8 OX=1175850 GN=E6 PE=3 SV=1
MM1 pKa = 7.29 ARR3 pKa = 11.84 KK4 pKa = 9.9 EE5 pKa = 4.03 EE6 pKa = 4.28 TQEE9 pKa = 3.98 SLTEE13 pKa = 4.23 RR14 pKa = 11.84 FDD16 pKa = 4.04 ALQEE20 pKa = 4.78 AILTMIEE27 pKa = 4.09 ANATDD32 pKa = 4.05 LASHH36 pKa = 6.8 LKK38 pKa = 9.06 YY39 pKa = 9.38 WDD41 pKa = 3.5 YY42 pKa = 11.33 VRR44 pKa = 11.84 KK45 pKa = 10.18 EE46 pKa = 4.0 NVTLYY51 pKa = 10.2 YY52 pKa = 10.58 ARR54 pKa = 11.84 KK55 pKa = 9.46 CGYY58 pKa = 9.62 NRR60 pKa = 11.84 LGLQPTPTPAVSEE73 pKa = 4.46 YY74 pKa = 10.35 NAKK77 pKa = 9.88 QAIKK81 pKa = 8.62 MQMLIRR87 pKa = 11.84 SLMTSQYY94 pKa = 11.37 ADD96 pKa = 3.56 EE97 pKa = 5.09 HH98 pKa = 5.82 WTLSDD103 pKa = 3.49 ISAEE107 pKa = 4.35 VINTQPRR114 pKa = 11.84 DD115 pKa = 3.59 CFKK118 pKa = 10.73 KK119 pKa = 9.51 QGYY122 pKa = 6.22 TVEE125 pKa = 3.65 VWYY128 pKa = 10.47 DD129 pKa = 3.29 NDD131 pKa = 3.41 RR132 pKa = 11.84 SKK134 pKa = 11.55 AFPYY138 pKa = 9.83 TNWTAIYY145 pKa = 9.96 YY146 pKa = 9.87 QDD148 pKa = 5.36 EE149 pKa = 4.23 NGNWHH154 pKa = 5.62 KK155 pKa = 9.92 TEE157 pKa = 4.05 GQVDD161 pKa = 4.01 ANGMYY166 pKa = 10.0 YY167 pKa = 10.19 KK168 pKa = 10.2 EE169 pKa = 4.28 VNGDD173 pKa = 3.34 LVYY176 pKa = 10.35 FHH178 pKa = 7.19 LFGPDD183 pKa = 3.03 AEE185 pKa = 5.15 KK186 pKa = 11.11 YY187 pKa = 10.45 GSTGEE192 pKa = 4.07 WTVHH196 pKa = 5.99 FKK198 pKa = 10.86 QSTIVSSSSSFAKK211 pKa = 10.43 RR212 pKa = 11.84 SPEE215 pKa = 3.49 PLKK218 pKa = 10.69 RR219 pKa = 11.84 KK220 pKa = 9.49 RR221 pKa = 11.84 DD222 pKa = 3.57 EE223 pKa = 4.57 SSPSTSWDD231 pKa = 3.78 TPSTTQDD238 pKa = 2.59 IVEE241 pKa = 4.3 RR242 pKa = 11.84 RR243 pKa = 11.84 SEE245 pKa = 4.04 RR246 pKa = 11.84 QTTVSSSSEE255 pKa = 3.64 ANLRR259 pKa = 11.84 PRR261 pKa = 11.84 RR262 pKa = 11.84 GGEE265 pKa = 3.72 QGEE268 pKa = 4.61 LPTTTKK274 pKa = 10.27 RR275 pKa = 11.84 RR276 pKa = 11.84 RR277 pKa = 11.84 LQSEE281 pKa = 4.44 LSAVPTPDD289 pKa = 3.2 QVGTRR294 pKa = 11.84 HH295 pKa = 6.27 RR296 pKa = 11.84 SAPRR300 pKa = 11.84 SNLTRR305 pKa = 11.84 LQRR308 pKa = 11.84 LQIEE312 pKa = 4.48 SWDD315 pKa = 3.79 PPILIVKK322 pKa = 7.33 GHH324 pKa = 6.62 PNSLKK329 pKa = 9.9 CWRR332 pKa = 11.84 NRR334 pKa = 11.84 LRR336 pKa = 11.84 STANSLLYY344 pKa = 10.11 EE345 pKa = 4.18 YY346 pKa = 11.07 SSTVWKK352 pKa = 9.91 WIGDD356 pKa = 3.81 NNNLLGSRR364 pKa = 11.84 MLLSFTSVTQRR375 pKa = 11.84 NQFLKK380 pKa = 10.45 VVKK383 pKa = 9.22 PPKK386 pKa = 10.25 GSVFALGSLDD396 pKa = 3.67 SLL398 pKa = 4.35
Molecular weight: 45.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.582
IPC2_protein 8.551
IPC_protein 8.492
Toseland 9.004
ProMoST 8.916
Dawson 9.355
Bjellqvist 9.165
Wikipedia 9.56
Rodwell 9.487
Grimsley 9.428
Solomon 9.414
Lehninger 9.355
Nozaki 9.165
DTASelect 9.107
Thurlkill 9.18
EMBOSS 9.458
Sillero 9.326
Patrickios 4.609
IPC_peptide 9.399
IPC2_peptide 7.805
IPC2.peptide.svr19 7.688
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2421
97
605
345.9
39.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.204 ± 0.555
1.983 ± 0.576
6.444 ± 0.37
6.237 ± 0.565
4.75 ± 0.505
5.411 ± 0.466
1.983 ± 0.254
5.494 ± 0.803
5.328 ± 0.756
9.583 ± 0.721
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.528 ± 0.19
5.081 ± 0.337
5.989 ± 0.902
4.585 ± 0.575
5.37 ± 0.669
7.683 ± 0.596
6.939 ± 0.498
5.618 ± 0.544
1.363 ± 0.356
3.428 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here