Streptococcus satellite phage Javan142

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZEC1|A0A4D5ZEC1_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan142 OX=2558539 GN=JavanS142_0004 PE=4 SV=1
MM1 pKa = 7.7AVFNQFWAYY10 pKa = 7.59QTLFQPTAQDD20 pKa = 2.62IKK22 pKa = 11.07LAILGYY28 pKa = 8.48FTGTLWACEE37 pKa = 4.0VSVLEE42 pKa = 4.23DD43 pKa = 3.44VEE45 pKa = 4.96LGYY48 pKa = 10.72HH49 pKa = 5.97VIVSYY54 pKa = 9.59VSCFDD59 pKa = 3.68KK60 pKa = 10.88SDD62 pKa = 3.75RR63 pKa = 11.84LLKK66 pKa = 10.14IAQAVEE72 pKa = 3.87NSQLGG77 pKa = 3.51

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZF60|A0A4D5ZF60_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan142 OX=2558539 GN=JavanS142_0011 PE=4 SV=1
MM1 pKa = 7.1YY2 pKa = 7.81RR3 pKa = 11.84TSVYY7 pKa = 10.65LGVDD11 pKa = 2.96QVTGKK16 pKa = 10.41KK17 pKa = 10.32ARR19 pKa = 11.84TTITASTKK27 pKa = 10.32KK28 pKa = 10.14GVKK31 pKa = 9.43IKK33 pKa = 10.92ARR35 pKa = 11.84DD36 pKa = 3.59ALNNFAMNGYY46 pKa = 6.25TVKK49 pKa = 10.52EE50 pKa = 4.27KK51 pKa = 9.63PTVTTYY57 pKa = 11.25KK58 pKa = 10.56EE59 pKa = 4.08LTALWWEE66 pKa = 4.56SYY68 pKa = 11.25KK69 pKa = 10.03NTIKK73 pKa = 10.76PNSRR77 pKa = 11.84QSMEE81 pKa = 4.4GIVRR85 pKa = 11.84LHH87 pKa = 6.69ILPAFGDD94 pKa = 4.16CKK96 pKa = 10.5LSRR99 pKa = 11.84LTTPVIQQQVNKK111 pKa = 8.75WANNANKK118 pKa = 10.06GIKK121 pKa = 9.07GAYY124 pKa = 9.42ANYY127 pKa = 10.79SFLNNINRR135 pKa = 11.84RR136 pKa = 11.84ILQYY140 pKa = 10.72GVTMQVIEE148 pKa = 4.63HH149 pKa = 6.18NPARR153 pKa = 11.84DD154 pKa = 3.48VIIPRR159 pKa = 11.84KK160 pKa = 9.5QNNKK164 pKa = 5.23EE165 pKa = 3.95HH166 pKa = 6.3KK167 pKa = 10.35VKK169 pKa = 10.58FFSNQEE175 pKa = 3.7LKK177 pKa = 10.88QFLNYY182 pKa = 10.28LDD184 pKa = 5.7DD185 pKa = 5.87LDD187 pKa = 4.43LSSYY191 pKa = 11.49EE192 pKa = 3.95NFFDD196 pKa = 3.77YY197 pKa = 11.08VLYY200 pKa = 8.87KK201 pKa = 10.31TLLATGCRR209 pKa = 11.84IGEE212 pKa = 4.15VLALEE217 pKa = 4.36WSDD220 pKa = 3.01IDD222 pKa = 5.15LEE224 pKa = 4.49KK225 pKa = 10.79GTIKK229 pKa = 10.46VSKK232 pKa = 8.99TLNRR236 pKa = 11.84YY237 pKa = 8.7QEE239 pKa = 4.35TNTPKK244 pKa = 10.61SKK246 pKa = 10.87AGLRR250 pKa = 11.84DD251 pKa = 3.37IEE253 pKa = 4.11IDD255 pKa = 3.31RR256 pKa = 11.84ATVLLLKK263 pKa = 10.19QYY265 pKa = 11.23KK266 pKa = 9.66NRR268 pKa = 11.84QQVLSWDD275 pKa = 3.8LGRR278 pKa = 11.84SEE280 pKa = 5.22TIVFTPFTTKK290 pKa = 10.34YY291 pKa = 10.75AYY293 pKa = 10.29ACLLRR298 pKa = 11.84KK299 pKa = 9.64RR300 pKa = 11.84LQKK303 pKa = 10.24HH304 pKa = 5.51FKK306 pKa = 10.01AAGVPDD312 pKa = 3.77ISFHH316 pKa = 5.86GFRR319 pKa = 11.84HH320 pKa = 4.4THH322 pKa = 4.65ATIMLYY328 pKa = 10.62AGIEE332 pKa = 4.18AKK334 pKa = 10.42DD335 pKa = 3.35LQYY338 pKa = 11.55RR339 pKa = 11.84LGHH342 pKa = 6.02SNISMTLNTYY352 pKa = 8.7VHH354 pKa = 6.14ATKK357 pKa = 10.47EE358 pKa = 4.25GAKK361 pKa = 9.7KK362 pKa = 10.18AVSIFEE368 pKa = 4.16TAISNLL374 pKa = 3.59

Molecular weight: 42.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

3237

39

537

129.5

15.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.653 ± 0.614

0.587 ± 0.142

5.962 ± 0.451

8.403 ± 0.771

4.14 ± 0.382

4.603 ± 0.526

1.823 ± 0.197

6.58 ± 0.354

9.082 ± 0.469

9.886 ± 0.476

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.533 ± 0.247

5.252 ± 0.574

2.317 ± 0.189

4.665 ± 0.291

4.912 ± 0.377

5.653 ± 0.268

6.117 ± 0.707

5.931 ± 0.452

1.05 ± 0.154

4.85 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski