Bacteroides phage Barc2635

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M2YSL2|A0A6M2YSL2_9CAUD Uncharacterized protein OS=Bacteroides phage Barc2635 OX=2596712 GN=BARC2635_0023 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 9.99EE3 pKa = 3.78IKK5 pKa = 10.14EE6 pKa = 4.34SEE8 pKa = 4.32LNTLLEE14 pKa = 4.53NGDD17 pKa = 3.8LCDD20 pKa = 3.27YY21 pKa = 10.31CPYY24 pKa = 10.55VRR26 pKa = 11.84GEE28 pKa = 3.97ISKK31 pKa = 10.62GLYY34 pKa = 9.47DD35 pKa = 3.91LCEE38 pKa = 3.98GCYY41 pKa = 10.3CEE43 pKa = 4.26QAKK46 pKa = 10.77DD47 pKa = 3.67NYY49 pKa = 10.54VLEE52 pKa = 4.6NDD54 pKa = 4.23LDD56 pKa = 4.07YY57 pKa = 11.95EE58 pKa = 4.35EE59 pKa = 5.0

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M2YSL3|A0A6M2YSL3_9CAUD Uncharacterized protein OS=Bacteroides phage Barc2635 OX=2596712 GN=BARC2635_0025 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 10.51NRR4 pKa = 11.84FMKK7 pKa = 10.61RR8 pKa = 11.84LIGYY12 pKa = 7.3SHH14 pKa = 6.62YY15 pKa = 10.67VDD17 pKa = 3.24KK18 pKa = 11.06LVYY21 pKa = 8.87VTVDD25 pKa = 3.29GGRR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 3.95IMKK33 pKa = 9.95LKK35 pKa = 9.66PRR37 pKa = 11.84KK38 pKa = 9.38KK39 pKa = 9.95KK40 pKa = 10.28FYY42 pKa = 10.94NSLEE46 pKa = 4.14YY47 pKa = 10.94YY48 pKa = 10.47KK49 pKa = 10.46AIQKK53 pKa = 8.53MFEE56 pKa = 4.04GG57 pKa = 4.24

Molecular weight: 7.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14318

57

1860

213.7

24.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.797 ± 0.413

1.516 ± 0.199

5.741 ± 0.206

8.109 ± 0.467

4.47 ± 0.23

5.902 ± 0.405

1.285 ± 0.109

6.991 ± 0.228

8.947 ± 0.558

8.011 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.417 ± 0.225

5.748 ± 0.158

2.766 ± 0.216

3.506 ± 0.239

4.638 ± 0.3

6.607 ± 0.264

5.685 ± 0.477

6.349 ± 0.292

0.936 ± 0.096

4.582 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski