Escherichia phage Qbeta

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Leviviricetes; Norzivirales; Fiersviridae; Qubevirus; Qubevirus durum

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O64308|RDRP_BPMX1 RNA-directed RNA polymerase subunit beta OS=Escherichia phage Qbeta OX=2789016 PE=3 SV=1
MM1 pKa = 7.83AKK3 pKa = 9.99LQAITLSGIGKK14 pKa = 9.75NGDD17 pKa = 3.02VTLNLNPRR25 pKa = 11.84GVNPTNGVAALSEE38 pKa = 4.39AGAVPALEE46 pKa = 3.98KK47 pKa = 10.53RR48 pKa = 11.84VTISVSQPSRR58 pKa = 11.84NRR60 pKa = 11.84KK61 pKa = 8.01NYY63 pKa = 8.78KK64 pKa = 9.53VQVKK68 pKa = 9.25IQNPTSCTASGTCDD82 pKa = 3.32PSVTRR87 pKa = 11.84SAYY90 pKa = 10.58ADD92 pKa = 3.26VTFSFTQYY100 pKa = 9.75STDD103 pKa = 3.39EE104 pKa = 3.79EE105 pKa = 4.42RR106 pKa = 11.84ALVRR110 pKa = 11.84TEE112 pKa = 4.54LKK114 pKa = 10.83ALLADD119 pKa = 4.12PMLIDD124 pKa = 6.35AIDD127 pKa = 4.13NLNPAYY133 pKa = 7.22WTALLGDD140 pKa = 4.32GSGPSPVPGPNPDD153 pKa = 4.17PPLEE157 pKa = 4.47PPPGTGSYY165 pKa = 8.32TCPFRR170 pKa = 11.84IWDD173 pKa = 3.61LSSIYY178 pKa = 10.02EE179 pKa = 4.18AANSSHH185 pKa = 6.43SWDD188 pKa = 3.15IYY190 pKa = 10.68NAVEE194 pKa = 3.84LSPRR198 pKa = 11.84KK199 pKa = 9.52FDD201 pKa = 3.53VTLDD205 pKa = 3.87DD206 pKa = 5.62LLGNTDD212 pKa = 2.64WRR214 pKa = 11.84DD215 pKa = 2.46WDD217 pKa = 3.22GRR219 pKa = 11.84LRR221 pKa = 11.84YY222 pKa = 6.21TTFRR226 pKa = 11.84GSRR229 pKa = 11.84GNGYY233 pKa = 9.6IDD235 pKa = 5.51LDD237 pKa = 4.06ATSLMQDD244 pKa = 3.34EE245 pKa = 5.28YY246 pKa = 10.62LTSSKK251 pKa = 11.06YY252 pKa = 10.33LVRR255 pKa = 11.84EE256 pKa = 4.44GKK258 pKa = 10.38RR259 pKa = 11.84PGAFGSIEE267 pKa = 3.94RR268 pKa = 11.84FVYY271 pKa = 10.21LKK273 pKa = 10.67SINAYY278 pKa = 10.16CSLSDD283 pKa = 2.99ITAYY287 pKa = 10.59HH288 pKa = 6.45SDD290 pKa = 3.39GVVVGFWRR298 pKa = 11.84DD299 pKa = 3.36PSSGGAIPFDD309 pKa = 3.82FSEE312 pKa = 4.63FDD314 pKa = 3.73SNKK317 pKa = 9.97CPIQAVIVVPRR328 pKa = 11.84LL329 pKa = 3.22

Molecular weight: 35.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O64307|A1_BPMX1 Minor capsid protein A1 OS=Escherichia phage Qbeta OX=2789016 PE=3 SV=1
MM1 pKa = 7.49PRR3 pKa = 11.84LPRR6 pKa = 11.84ALRR9 pKa = 11.84FGPNMEE15 pKa = 4.23VLSDD19 pKa = 4.32FQEE22 pKa = 3.67LWYY25 pKa = 9.69PEE27 pKa = 4.64SIIDD31 pKa = 4.36SDD33 pKa = 3.96VKK35 pKa = 10.95YY36 pKa = 10.31PLYY39 pKa = 10.23TFRR42 pKa = 11.84GSIGGSFFDD51 pKa = 5.17SYY53 pKa = 10.33GTNNIVRR60 pKa = 11.84EE61 pKa = 4.17IRR63 pKa = 11.84RR64 pKa = 11.84TPHH67 pKa = 6.52CATVPIASSGLRR79 pKa = 11.84PCTSVWYY86 pKa = 9.94DD87 pKa = 3.32PTSLLFRR94 pKa = 11.84IPEE97 pKa = 3.88MRR99 pKa = 11.84AEE101 pKa = 4.24WDD103 pKa = 3.2NGMGDD108 pKa = 4.07AGDD111 pKa = 3.49IVYY114 pKa = 10.64KK115 pKa = 10.77DD116 pKa = 4.03FLFSTPAPKK125 pKa = 10.6EE126 pKa = 3.76FDD128 pKa = 3.83FSNSLAPRR136 pKa = 11.84YY137 pKa = 10.3SNAFSAFNAKK147 pKa = 9.9YY148 pKa = 10.36GVIIGEE154 pKa = 4.02GHH156 pKa = 5.48EE157 pKa = 4.18TLKK160 pKa = 11.11YY161 pKa = 9.87FALLLRR167 pKa = 11.84RR168 pKa = 11.84LHH170 pKa = 5.78KK171 pKa = 10.13AVRR174 pKa = 11.84AVRR177 pKa = 11.84HH178 pKa = 5.48GDD180 pKa = 3.2LRR182 pKa = 11.84GLRR185 pKa = 11.84KK186 pKa = 9.57ILDD189 pKa = 3.91SYY191 pKa = 11.85NKK193 pKa = 9.9GRR195 pKa = 11.84WKK197 pKa = 10.18PATAGNLWLEE207 pKa = 4.14FRR209 pKa = 11.84YY210 pKa = 10.65GLTPLFHH217 pKa = 7.6DD218 pKa = 4.61IKK220 pKa = 11.2SVMDD224 pKa = 3.35DD225 pKa = 3.07WNRR228 pKa = 11.84INDD231 pKa = 4.36KK232 pKa = 10.01IQKK235 pKa = 9.24LRR237 pKa = 11.84RR238 pKa = 11.84FSVGHH243 pKa = 6.33GEE245 pKa = 4.18DD246 pKa = 3.63FKK248 pKa = 11.76LSIDD252 pKa = 3.54GLYY255 pKa = 10.28PGLTHH260 pKa = 6.94FRR262 pKa = 11.84LSGEE266 pKa = 3.67ITVQRR271 pKa = 11.84RR272 pKa = 11.84HH273 pKa = 5.28RR274 pKa = 11.84WGITYY279 pKa = 10.49ANRR282 pKa = 11.84EE283 pKa = 4.14GYY285 pKa = 8.46ATFDD289 pKa = 3.42NGSIRR294 pKa = 11.84PVSDD298 pKa = 2.51WKK300 pKa = 10.89EE301 pKa = 3.5LANAFINPGEE311 pKa = 4.46VAWEE315 pKa = 3.73LTPYY319 pKa = 10.82SFIVDD324 pKa = 2.97WFINVGDD331 pKa = 4.74IIEE334 pKa = 4.29QQKK337 pKa = 9.3QWYY340 pKa = 8.36QNIDD344 pKa = 3.11IVDD347 pKa = 4.76GYY349 pKa = 8.32QRR351 pKa = 11.84RR352 pKa = 11.84DD353 pKa = 2.52IRR355 pKa = 11.84MRR357 pKa = 11.84SVSLKK362 pKa = 9.88GVRR365 pKa = 11.84NGIPVRR371 pKa = 11.84VTGSVEE377 pKa = 4.25LVDD380 pKa = 3.9SFYY383 pKa = 11.43NRR385 pKa = 11.84SHH387 pKa = 5.49TTRR390 pKa = 11.84IPQATLAIDD399 pKa = 3.55TSFSSIKK406 pKa = 10.38HH407 pKa = 5.03VMDD410 pKa = 5.31SISLITQRR418 pKa = 11.84IKK420 pKa = 10.83RR421 pKa = 3.79

Molecular weight: 48.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1469

133

586

367.3

41.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.739 ± 0.871

1.566 ± 0.43

6.399 ± 0.379

4.493 ± 0.39

4.493 ± 0.686

6.807 ± 0.356

1.361 ± 0.415

6.059 ± 0.654

4.289 ± 0.194

8.986 ± 0.523

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.498 ± 0.168

4.969 ± 0.43

5.582 ± 0.61

2.246 ± 0.257

7.42 ± 0.729

8.918 ± 0.417

5.718 ± 0.681

6.739 ± 0.401

1.77 ± 0.362

3.948 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski