Labidocera aestiva circovirus
Average proteome isoelectric point is 8.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M3VMK9|M3VMK9_9CIRC Putative viral replication protein OS=Labidocera aestiva circovirus OX=1173709 GN=LaCopCV_gp1 PE=4 SV=1
MM1 pKa = 7.38 SRR3 pKa = 11.84 NFTGTVNNPTQTLEE17 pKa = 3.69 EE18 pKa = 4.08 WLATLKK24 pKa = 10.64 RR25 pKa = 11.84 LPHH28 pKa = 5.64 ATAARR33 pKa = 11.84 CQLEE37 pKa = 4.11 KK38 pKa = 11.31 GEE40 pKa = 4.66 NGTPHH45 pKa = 6.58 IQWCVTLSKK54 pKa = 10.45 VARR57 pKa = 11.84 LTGIIKK63 pKa = 10.3 KK64 pKa = 10.05 LAGSHH69 pKa = 5.79 VEE71 pKa = 3.8 ASRR74 pKa = 11.84 NAMAAWKK81 pKa = 10.25 YY82 pKa = 10.17 CGKK85 pKa = 10.26 EE86 pKa = 3.87 DD87 pKa = 3.86 TRR89 pKa = 11.84 VEE91 pKa = 4.31 GPLEE95 pKa = 4.35 FGVPPASKK103 pKa = 9.9 RR104 pKa = 11.84 VKK106 pKa = 10.72 GDD108 pKa = 3.14 TKK110 pKa = 10.74 EE111 pKa = 3.94 RR112 pKa = 11.84 NKK114 pKa = 10.23 MILEE118 pKa = 4.91 LGPVAAVEE126 pKa = 3.93 QGLIPVEE133 pKa = 3.82 KK134 pKa = 10.26 FKK136 pKa = 10.93 AIKK139 pKa = 9.86 QSCDD143 pKa = 2.7 LFAVMKK149 pKa = 10.61 KK150 pKa = 9.24 EE151 pKa = 3.88 VHH153 pKa = 5.35 TTDD156 pKa = 3.27 ALSNEE161 pKa = 3.64 WHH163 pKa = 6.36 YY164 pKa = 11.81 GEE166 pKa = 5.27 SGTGKK171 pKa = 9.89 SRR173 pKa = 11.84 GVRR176 pKa = 11.84 EE177 pKa = 4.16 RR178 pKa = 11.84 FPDD181 pKa = 3.63 AFIKK185 pKa = 10.93 SSDD188 pKa = 3.68 VWWDD192 pKa = 3.88 GYY194 pKa = 10.79 QGEE197 pKa = 4.36 EE198 pKa = 3.89 TVIIEE203 pKa = 4.32 EE204 pKa = 4.52 MGPKK208 pKa = 9.96 QIGAHH213 pKa = 5.74 HH214 pKa = 6.52 LKK216 pKa = 9.61 IWSDD220 pKa = 3.47 HH221 pKa = 5.78 YY222 pKa = 10.88 PFKK225 pKa = 10.71 AAQKK229 pKa = 10.32 GSQLLIRR236 pKa = 11.84 PKK238 pKa = 10.44 RR239 pKa = 11.84 IIVTSNYY246 pKa = 9.94 SIKK249 pKa = 10.41 EE250 pKa = 4.15 CYY252 pKa = 8.72 EE253 pKa = 4.01 AEE255 pKa = 4.04
Molecular weight: 28.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.144
IPC2_protein 8.141
IPC_protein 8.053
Toseland 8.536
ProMoST 8.58
Dawson 8.946
Bjellqvist 8.975
Wikipedia 8.975
Rodwell 9.121
Grimsley 8.507
Solomon 9.092
Lehninger 9.092
Nozaki 9.224
DTASelect 8.814
Thurlkill 8.96
EMBOSS 9.151
Sillero 9.165
Patrickios 4.927
IPC_peptide 9.092
IPC2_peptide 7.673
IPC2.peptide.svr19 7.682
Protein with the highest isoelectric point:
>tr|M3VMK9|M3VMK9_9CIRC Putative viral replication protein OS=Labidocera aestiva circovirus OX=1173709 GN=LaCopCV_gp1 PE=4 SV=1
MM1 pKa = 7.34 PARR4 pKa = 11.84 RR5 pKa = 11.84 SFRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.13 SAPKK15 pKa = 9.41 RR16 pKa = 11.84 RR17 pKa = 11.84 ARR19 pKa = 11.84 GRR21 pKa = 11.84 KK22 pKa = 8.32 ARR24 pKa = 11.84 ARR26 pKa = 11.84 RR27 pKa = 11.84 SPFNSSWYY35 pKa = 9.29 HH36 pKa = 6.12 AKK38 pKa = 10.53 CQVTFPIEE46 pKa = 4.05 HH47 pKa = 6.6 NNNYY51 pKa = 9.9 AQGSDD56 pKa = 3.45 TTCARR61 pKa = 11.84 FVVKK65 pKa = 10.33 WNDD68 pKa = 3.08 SSNDD72 pKa = 3.47 DD73 pKa = 3.38 MATPQVAVLRR83 pKa = 11.84 NSRR86 pKa = 11.84 QWQALAAQFRR96 pKa = 11.84 EE97 pKa = 4.22 YY98 pKa = 10.17 TVRR101 pKa = 11.84 GVKK104 pKa = 9.48 IEE106 pKa = 4.2 YY107 pKa = 9.46 KK108 pKa = 10.14 GHH110 pKa = 6.07 NAVGGDD116 pKa = 3.21 KK117 pKa = 10.08 EE118 pKa = 4.33 YY119 pKa = 10.58 RR120 pKa = 11.84 ASHH123 pKa = 5.71 VWSDD127 pKa = 3.23 PDD129 pKa = 3.87 VYY131 pKa = 11.01 VAPTEE136 pKa = 4.22 AGNDD140 pKa = 3.56 GAYY143 pKa = 10.3 FWDD146 pKa = 3.36 INRR149 pKa = 11.84 FIQKK153 pKa = 9.42 PDD155 pKa = 3.58 YY156 pKa = 11.14 KK157 pKa = 10.76 EE158 pKa = 3.83 MSHH161 pKa = 6.11 NRR163 pKa = 11.84 SFTKK167 pKa = 10.73 YY168 pKa = 8.45 IGVEE172 pKa = 3.66 KK173 pKa = 9.49 FHH175 pKa = 7.07 RR176 pKa = 11.84 RR177 pKa = 11.84 RR178 pKa = 11.84 GLNHH182 pKa = 5.06 THH184 pKa = 6.18 RR185 pKa = 11.84 TLAQISTPHH194 pKa = 5.96 CTSFQLNSRR203 pKa = 11.84 GFDD206 pKa = 3.19 NGHH209 pKa = 6.42 DD210 pKa = 3.69 MGRR213 pKa = 11.84 VTVTWFVIFKK223 pKa = 10.58 QPAFF227 pKa = 3.7
Molecular weight: 26.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.721
IPC_protein 10.452
Toseland 10.526
ProMoST 10.233
Dawson 10.672
Bjellqvist 10.379
Wikipedia 10.877
Rodwell 10.921
Grimsley 10.73
Solomon 10.745
Lehninger 10.716
Nozaki 10.526
DTASelect 10.379
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.599
Patrickios 10.599
IPC_peptide 10.745
IPC2_peptide 9.326
IPC2.peptide.svr19 8.595
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
482
227
255
241.0
27.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.506 ± 0.18
1.66 ± 0.2
4.149 ± 0.672
6.224 ± 1.857
4.357 ± 1.071
6.846 ± 0.662
3.734 ± 0.397
4.564 ± 0.875
7.676 ± 1.153
4.564 ± 1.396
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.867 ± 0.062
4.564 ± 0.948
4.564 ± 0.094
3.942 ± 0.274
8.091 ± 1.988
6.432 ± 0.365
6.017 ± 0.171
6.432 ± 0.104
2.697 ± 0.032
3.112 ± 0.504
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here