Amino acid dipepetide frequency for Streptomyces sp. 69

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.589AlaAla: 20.589 ± 0.151
1.092AlaCys: 1.092 ± 0.024
8.228AlaAsp: 8.228 ± 0.067
8.915AlaGlu: 8.915 ± 0.087
3.452AlaPhe: 3.452 ± 0.041
12.426AlaGly: 12.426 ± 0.074
3.047AlaHis: 3.047 ± 0.035
3.424AlaIle: 3.424 ± 0.042
2.939AlaLys: 2.939 ± 0.042
14.356AlaLeu: 14.356 ± 0.11
2.452AlaMet: 2.452 ± 0.035
1.909AlaAsn: 1.909 ± 0.03
7.05AlaPro: 7.05 ± 0.081
3.925AlaGln: 3.925 ± 0.045
10.325AlaArg: 10.325 ± 0.082
5.924AlaSer: 5.924 ± 0.055
7.074AlaThr: 7.074 ± 0.066
12.006AlaVal: 12.006 ± 0.091
1.895AlaTrp: 1.895 ± 0.033
2.84AlaTyr: 2.84 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
1.103CysAla: 1.103 ± 0.024
0.114CysCys: 0.114 ± 0.008
0.466CysAsp: 0.466 ± 0.013
0.426CysGlu: 0.426 ± 0.014
0.24CysPhe: 0.24 ± 0.01
0.97CysGly: 0.97 ± 0.023
0.218CysHis: 0.218 ± 0.009
0.174CysIle: 0.174 ± 0.009
0.123CysLys: 0.123 ± 0.007
0.795CysLeu: 0.795 ± 0.022
0.137CysMet: 0.137 ± 0.007
0.142CysAsn: 0.142 ± 0.008
0.511CysPro: 0.511 ± 0.015
0.178CysGln: 0.178 ± 0.009
0.613CysArg: 0.613 ± 0.015
0.45CysSer: 0.45 ± 0.015
0.549CysThr: 0.549 ± 0.016
0.678CysVal: 0.678 ± 0.018
0.15CysTrp: 0.15 ± 0.007
0.149CysTyr: 0.149 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.534AspAla: 7.534 ± 0.064
0.429AspCys: 0.429 ± 0.014
3.584AspAsp: 3.584 ± 0.049
3.853AspGlu: 3.853 ± 0.042
1.687AspPhe: 1.687 ± 0.026
6.152AspGly: 6.152 ± 0.071
1.483AspHis: 1.483 ± 0.024
1.909AspIle: 1.909 ± 0.029
1.251AspLys: 1.251 ± 0.025
6.33AspLeu: 6.33 ± 0.06
0.844AspMet: 0.844 ± 0.018
1.011AspAsn: 1.011 ± 0.023
4.541AspPro: 4.541 ± 0.05
1.643AspGln: 1.643 ± 0.026
4.822AspArg: 4.822 ± 0.054
2.587AspSer: 2.587 ± 0.034
3.351AspThr: 3.351 ± 0.038
4.74AspVal: 4.74 ± 0.053
1.023AspTrp: 1.023 ± 0.02
1.107AspTyr: 1.107 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
7.422GluAla: 7.422 ± 0.079
0.393GluCys: 0.393 ± 0.015
2.873GluAsp: 2.873 ± 0.042
3.692GluGlu: 3.692 ± 0.047
1.447GluPhe: 1.447 ± 0.026
4.333GluGly: 4.333 ± 0.044
1.588GluHis: 1.588 ± 0.025
2.231GluIle: 2.231 ± 0.032
1.49GluLys: 1.49 ± 0.027
6.679GluLeu: 6.679 ± 0.063
0.885GluMet: 0.885 ± 0.023
1.038GluAsn: 1.038 ± 0.023
3.393GluPro: 3.393 ± 0.043
2.353GluGln: 2.353 ± 0.036
5.575GluArg: 5.575 ± 0.056
2.51GluSer: 2.51 ± 0.038
2.944GluThr: 2.944 ± 0.038
4.409GluVal: 4.409 ± 0.051
0.797GluTrp: 0.797 ± 0.019
1.115GluTyr: 1.115 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
3.595PheAla: 3.595 ± 0.039
0.279PheCys: 0.279 ± 0.01
1.89PheAsp: 1.89 ± 0.032
1.466PheGlu: 1.466 ± 0.025
0.872PhePhe: 0.872 ± 0.022
2.976PheGly: 2.976 ± 0.041
0.617PheHis: 0.617 ± 0.016
0.671PheIle: 0.671 ± 0.018
0.546PheLys: 0.546 ± 0.016
2.625PheLeu: 2.625 ± 0.035
0.387PheMet: 0.387 ± 0.014
0.565PheAsn: 0.565 ± 0.014
1.403PhePro: 1.403 ± 0.022
0.7PheGln: 0.7 ± 0.016
1.815PheArg: 1.815 ± 0.026
1.456PheSer: 1.456 ± 0.024
2.001PheThr: 2.001 ± 0.032
2.232PheVal: 2.232 ± 0.03
0.396PheTrp: 0.396 ± 0.012
0.58PheTyr: 0.58 ± 0.014
0.0PheXaa: 0.0 ± 0.0
Gly
10.718GlyAla: 10.718 ± 0.077
0.857GlyCys: 0.857 ± 0.021
5.155GlyAsp: 5.155 ± 0.047
4.923GlyGlu: 4.923 ± 0.056
2.835GlyPhe: 2.835 ± 0.032
8.547GlyGly: 8.547 ± 0.087
2.471GlyHis: 2.471 ± 0.034
3.404GlyIle: 3.404 ± 0.038
2.47GlyLys: 2.47 ± 0.041
9.249GlyLeu: 9.249 ± 0.074
1.963GlyMet: 1.963 ± 0.029
1.779GlyAsn: 1.779 ± 0.036
5.008GlyPro: 5.008 ± 0.052
2.709GlyGln: 2.709 ± 0.04
7.692GlyArg: 7.692 ± 0.067
5.296GlySer: 5.296 ± 0.055
6.464GlyThr: 6.464 ± 0.06
7.436GlyVal: 7.436 ± 0.066
1.72GlyTrp: 1.72 ± 0.03
2.292GlyTyr: 2.292 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
2.83HisAla: 2.83 ± 0.039
0.211HisCys: 0.211 ± 0.01
1.383HisAsp: 1.383 ± 0.025
1.267HisGlu: 1.267 ± 0.027
0.664HisPhe: 0.664 ± 0.016
2.535HisGly: 2.535 ± 0.033
0.757HisHis: 0.757 ± 0.02
0.724HisIle: 0.724 ± 0.017
0.408HisLys: 0.408 ± 0.014
2.499HisLeu: 2.499 ± 0.03
0.37HisMet: 0.37 ± 0.014
0.389HisAsn: 0.389 ± 0.012
1.96HisPro: 1.96 ± 0.033
0.687HisGln: 0.687 ± 0.018
2.178HisArg: 2.178 ± 0.031
1.04HisSer: 1.04 ± 0.02
1.424HisThr: 1.424 ± 0.023
1.793HisVal: 1.793 ± 0.026
0.392HisTrp: 0.392 ± 0.013
0.508HisTyr: 0.508 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
4.658IleAla: 4.658 ± 0.051
0.291IleCys: 0.291 ± 0.011
2.222IleAsp: 2.222 ± 0.034
2.0IleGlu: 2.0 ± 0.03
0.667IlePhe: 0.667 ± 0.017
3.513IleGly: 3.513 ± 0.043
0.638IleHis: 0.638 ± 0.017
0.867IleIle: 0.867 ± 0.018
0.759IleLys: 0.759 ± 0.02
2.441IleLeu: 2.441 ± 0.035
0.433IleMet: 0.433 ± 0.015
0.702IleAsn: 0.702 ± 0.017
1.816IlePro: 1.816 ± 0.027
0.788IleGln: 0.788 ± 0.018
2.276IleArg: 2.276 ± 0.027
1.637IleSer: 1.637 ± 0.026
2.252IleThr: 2.252 ± 0.032
2.719IleVal: 2.719 ± 0.036
0.384IleTrp: 0.384 ± 0.013
0.558IleTyr: 0.558 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
3.088LysAla: 3.088 ± 0.042
0.114LysCys: 0.114 ± 0.007
1.488LysAsp: 1.488 ± 0.032
1.228LysGlu: 1.228 ± 0.025
0.449LysPhe: 0.449 ± 0.015
1.963LysGly: 1.963 ± 0.033
0.475LysHis: 0.475 ± 0.015
0.89LysIle: 0.89 ± 0.021
0.925LysLys: 0.925 ± 0.029
2.061LysLeu: 2.061 ± 0.035
0.377LysMet: 0.377 ± 0.013
0.558LysAsn: 0.558 ± 0.017
1.398LysPro: 1.398 ± 0.028
0.771LysGln: 0.771 ± 0.018
1.493LysArg: 1.493 ± 0.029
1.145LysSer: 1.145 ± 0.025
1.384LysThr: 1.384 ± 0.031
1.967LysVal: 1.967 ± 0.033
0.271LysTrp: 0.271 ± 0.013
0.475LysTyr: 0.475 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
14.853LeuAla: 14.853 ± 0.106
0.842LeuCys: 0.842 ± 0.019
6.713LeuAsp: 6.713 ± 0.063
4.769LeuGlu: 4.769 ± 0.05
2.595LeuPhe: 2.595 ± 0.033
9.114LeuGly: 9.114 ± 0.073
2.347LeuHis: 2.347 ± 0.03
3.346LeuIle: 3.346 ± 0.041
2.168LeuLys: 2.168 ± 0.034
11.216LeuLeu: 11.216 ± 0.109
1.636LeuMet: 1.636 ± 0.029
1.732LeuAsn: 1.732 ± 0.026
6.543LeuPro: 6.543 ± 0.06
2.282LeuGln: 2.282 ± 0.031
8.679LeuArg: 8.679 ± 0.072
5.225LeuSer: 5.225 ± 0.052
6.863LeuThr: 6.863 ± 0.052
8.704LeuVal: 8.704 ± 0.068
1.316LeuTrp: 1.316 ± 0.027
1.928LeuTyr: 1.928 ± 0.029
0.0LeuXaa: 0.0 ± 0.0
Met
2.224MetAla: 2.224 ± 0.034
0.151MetCys: 0.151 ± 0.008
0.875MetAsp: 0.875 ± 0.02
0.763MetGlu: 0.763 ± 0.019
0.452MetPhe: 0.452 ± 0.015
1.297MetGly: 1.297 ± 0.027
0.36MetHis: 0.36 ± 0.012
0.65MetIle: 0.65 ± 0.017
0.422MetLys: 0.422 ± 0.013
1.651MetLeu: 1.651 ± 0.03
0.286MetMet: 0.286 ± 0.012
0.435MetAsn: 0.435 ± 0.013
1.124MetPro: 1.124 ± 0.024
0.467MetGln: 0.467 ± 0.013
1.418MetArg: 1.418 ± 0.026
1.34MetSer: 1.34 ± 0.022
1.556MetThr: 1.556 ± 0.025
1.288MetVal: 1.288 ± 0.022
0.216MetTrp: 0.216 ± 0.009
0.323MetTyr: 0.323 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.22AsnAla: 2.22 ± 0.033
0.168AsnCys: 0.168 ± 0.008
0.987AsnAsp: 0.987 ± 0.019
0.817AsnGlu: 0.817 ± 0.02
0.489AsnPhe: 0.489 ± 0.016
1.918AsnGly: 1.918 ± 0.032
0.417AsnHis: 0.417 ± 0.015
0.655AsnIle: 0.655 ± 0.016
0.43AsnLys: 0.43 ± 0.015
1.667AsnLeu: 1.667 ± 0.028
0.29AsnMet: 0.29 ± 0.01
0.45AsnAsn: 0.45 ± 0.017
1.439AsnPro: 1.439 ± 0.027
0.523AsnGln: 0.523 ± 0.016
1.268AsnArg: 1.268 ± 0.023
0.955AsnSer: 0.955 ± 0.022
1.15AsnThr: 1.15 ± 0.027
1.41AsnVal: 1.41 ± 0.027
0.305AsnTrp: 0.305 ± 0.013
0.406AsnTyr: 0.406 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.58ProAla: 8.58 ± 0.082
0.381ProCys: 0.381 ± 0.015
4.517ProAsp: 4.517 ± 0.048
4.414ProGlu: 4.414 ± 0.051
1.534ProPhe: 1.534 ± 0.023
6.573ProGly: 6.573 ± 0.068
1.502ProHis: 1.502 ± 0.027
1.272ProIle: 1.272 ± 0.022
1.248ProLys: 1.248 ± 0.027
5.4ProLeu: 5.4 ± 0.053
1.007ProMet: 1.007 ± 0.02
0.92ProAsn: 0.92 ± 0.023
3.599ProPro: 3.599 ± 0.058
1.784ProGln: 1.784 ± 0.033
4.064ProArg: 4.064 ± 0.047
3.357ProSer: 3.357 ± 0.044
3.354ProThr: 3.354 ± 0.045
5.466ProVal: 5.466 ± 0.049
0.918ProTrp: 0.918 ± 0.022
1.511ProTyr: 1.511 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.864GlnAla: 3.864 ± 0.045
0.18GlnCys: 0.18 ± 0.008
1.455GlnAsp: 1.455 ± 0.024
1.5GlnGlu: 1.5 ± 0.026
0.685GlnPhe: 0.685 ± 0.017
2.424GlnGly: 2.424 ± 0.034
0.721GlnHis: 0.721 ± 0.018
1.122GlnIle: 1.122 ± 0.021
0.674GlnLys: 0.674 ± 0.018
3.194GlnLeu: 3.194 ± 0.038
0.51GlnMet: 0.51 ± 0.015
0.516GlnAsn: 0.516 ± 0.015
1.826GlnPro: 1.826 ± 0.038
1.333GlnGln: 1.333 ± 0.034
2.41GlnArg: 2.41 ± 0.035
1.341GlnSer: 1.341 ± 0.027
1.441GlnThr: 1.441 ± 0.028
2.406GlnVal: 2.406 ± 0.035
0.48GlnTrp: 0.48 ± 0.016
0.654GlnTyr: 0.654 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
9.916ArgAla: 9.916 ± 0.084
0.654ArgCys: 0.654 ± 0.017
4.187ArgAsp: 4.187 ± 0.039
4.732ArgGlu: 4.732 ± 0.051
2.311ArgPhe: 2.311 ± 0.034
5.779ArgGly: 5.779 ± 0.051
2.211ArgHis: 2.211 ± 0.032
3.236ArgIle: 3.236 ± 0.04
1.695ArgLys: 1.695 ± 0.028
8.944ArgLeu: 8.944 ± 0.07
1.737ArgMet: 1.737 ± 0.028
1.361ArgAsn: 1.361 ± 0.025
5.046ArgPro: 5.046 ± 0.053
2.413ArgGln: 2.413 ± 0.036
7.884ArgArg: 7.884 ± 0.08
4.034ArgSer: 4.034 ± 0.044
5.47ArgThr: 5.47 ± 0.055
5.799ArgVal: 5.799 ± 0.048
1.372ArgTrp: 1.372 ± 0.027
1.841ArgTyr: 1.841 ± 0.026
0.0ArgXaa: 0.0 ± 0.0
Ser
6.839SerAla: 6.839 ± 0.057
0.434SerCys: 0.434 ± 0.014
2.718SerAsp: 2.718 ± 0.038
2.357SerGlu: 2.357 ± 0.033
1.478SerPhe: 1.478 ± 0.026
5.913SerGly: 5.913 ± 0.057
1.048SerHis: 1.048 ± 0.023
1.401SerIle: 1.401 ± 0.026
1.092SerLys: 1.092 ± 0.022
4.793SerLeu: 4.793 ± 0.053
1.085SerMet: 1.085 ± 0.022
0.877SerAsn: 0.877 ± 0.018
3.255SerPro: 3.255 ± 0.042
1.285SerGln: 1.285 ± 0.024
3.777SerArg: 3.777 ± 0.035
2.92SerSer: 2.92 ± 0.045
3.11SerThr: 3.11 ± 0.042
4.164SerVal: 4.164 ± 0.044
0.895SerTrp: 0.895 ± 0.021
1.299SerTyr: 1.299 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
8.856ThrAla: 8.856 ± 0.069
0.484ThrCys: 0.484 ± 0.015
3.743ThrAsp: 3.743 ± 0.043
3.356ThrGlu: 3.356 ± 0.039
1.613ThrPhe: 1.613 ± 0.028
6.699ThrGly: 6.699 ± 0.068
1.334ThrHis: 1.334 ± 0.027
1.753ThrIle: 1.753 ± 0.03
1.262ThrLys: 1.262 ± 0.026
5.819ThrLeu: 5.819 ± 0.054
0.947ThrMet: 0.947 ± 0.021
1.032ThrAsn: 1.032 ± 0.027
4.3ThrPro: 4.3 ± 0.046
1.435ThrGln: 1.435 ± 0.026
4.023ThrArg: 4.023 ± 0.038
3.341ThrSer: 3.341 ± 0.043
3.998ThrThr: 3.998 ± 0.055
6.065ThrVal: 6.065 ± 0.051
0.944ThrTrp: 0.944 ± 0.021
1.432ThrTyr: 1.432 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
10.494ValAla: 10.494 ± 0.081
0.768ValCys: 0.768 ± 0.019
4.871ValAsp: 4.871 ± 0.051
4.649ValGlu: 4.649 ± 0.051
2.429ValPhe: 2.429 ± 0.036
6.392ValGly: 6.392 ± 0.057
2.003ValHis: 2.003 ± 0.029
2.845ValIle: 2.845 ± 0.036
1.799ValLys: 1.799 ± 0.032
9.278ValLeu: 9.278 ± 0.076
1.407ValMet: 1.407 ± 0.024
1.69ValAsn: 1.69 ± 0.031
5.296ValPro: 5.296 ± 0.054
2.193ValGln: 2.193 ± 0.032
7.129ValArg: 7.129 ± 0.062
4.277ValSer: 4.277 ± 0.045
5.725ValThr: 5.725 ± 0.042
7.779ValVal: 7.779 ± 0.078
1.165ValTrp: 1.165 ± 0.025
1.616ValTyr: 1.616 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.691TrpAla: 1.691 ± 0.027
0.162TrpCys: 0.162 ± 0.009
0.843TrpAsp: 0.843 ± 0.021
0.719TrpGlu: 0.719 ± 0.02
0.512TrpPhe: 0.512 ± 0.017
1.071TrpGly: 1.071 ± 0.024
0.393TrpHis: 0.393 ± 0.013
0.555TrpIle: 0.555 ± 0.016
0.387TrpLys: 0.387 ± 0.013
1.791TrpLeu: 1.791 ± 0.032
0.286TrpMet: 0.286 ± 0.011
0.419TrpAsn: 0.419 ± 0.014
0.807TrpPro: 0.807 ± 0.02
0.636TrpGln: 0.636 ± 0.016
1.341TrpArg: 1.341 ± 0.026
0.933TrpSer: 0.933 ± 0.023
1.055TrpThr: 1.055 ± 0.024
1.006TrpVal: 1.006 ± 0.023
0.351TrpTrp: 0.351 ± 0.012
0.371TrpTyr: 0.371 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.791TyrAla: 2.791 ± 0.033
0.187TyrCys: 0.187 ± 0.008
1.685TyrAsp: 1.685 ± 0.034
1.323TyrGlu: 1.323 ± 0.025
0.662TyrPhe: 0.662 ± 0.019
2.391TyrGly: 2.391 ± 0.038
0.392TyrHis: 0.392 ± 0.013
0.491TyrIle: 0.491 ± 0.013
0.433TyrLys: 0.433 ± 0.013
2.083TyrLeu: 2.083 ± 0.031
0.257TyrMet: 0.257 ± 0.011
0.441TyrAsn: 0.441 ± 0.018
1.086TyrPro: 1.086 ± 0.022
0.63TyrGln: 0.63 ± 0.017
1.838TyrArg: 1.838 ± 0.026
0.969TyrSer: 0.969 ± 0.022
1.271TyrThr: 1.271 ± 0.026
1.736TyrVal: 1.736 ± 0.025
0.341TyrTrp: 0.341 ± 0.013
0.481TyrTyr: 0.481 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7647 proteins (2482473 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski