Arthrobacter livingstonensis
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4378 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V5LBC9|A0A2V5LBC9_9MICC Sugar ABC transporter permease OS=Arthrobacter livingstonensis OX=670078 GN=CVV68_07295 PE=3 SV=1
MM1 pKa = 7.38 SNIPAEE7 pKa = 4.07 LSYY10 pKa = 10.03 TAEE13 pKa = 4.41 HH14 pKa = 6.51 EE15 pKa = 4.51 WVGAPNADD23 pKa = 2.76 GVVRR27 pKa = 11.84 VGITDD32 pKa = 4.44 FAQDD36 pKa = 3.24 ALGDD40 pKa = 3.78 VVYY43 pKa = 11.22 VQMPDD48 pKa = 2.71 AGTAVTADD56 pKa = 3.58 TVVGEE61 pKa = 4.32 VEE63 pKa = 4.36 STKK66 pKa = 10.71 SVSDD70 pKa = 3.37 IYY72 pKa = 11.55 APLTGTVTARR82 pKa = 11.84 NDD84 pKa = 3.62 ALDD87 pKa = 4.71 ADD89 pKa = 4.28 PSLINSDD96 pKa = 3.71 PYY98 pKa = 10.01 GAGWLMEE105 pKa = 4.12 ITLADD110 pKa = 3.77 ATTHH114 pKa = 6.9 ASLLSAAAYY123 pKa = 8.2 QEE125 pKa = 4.37 KK126 pKa = 10.86 VGG128 pKa = 3.98
Molecular weight: 13.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.745
IPC_protein 3.719
Toseland 3.503
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.414
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.846
Patrickios 1.875
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A2V5L944|A0A2V5L944_9MICC Uncharacterized protein OS=Arthrobacter livingstonensis OX=670078 GN=CVV68_07780 PE=4 SV=1
MM1 pKa = 7.28 GSVVKK6 pKa = 10.44 KK7 pKa = 9.48 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.32 RR11 pKa = 11.84 MSKK14 pKa = 9.94 KK15 pKa = 9.5 KK16 pKa = 9.79 HH17 pKa = 5.45 RR18 pKa = 11.84 KK19 pKa = 6.15 QLRR22 pKa = 11.84 KK23 pKa = 7.92 TRR25 pKa = 11.84 HH26 pKa = 3.43 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4378
0
4378
1399206
25
3512
319.6
34.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.323 ± 0.059
0.638 ± 0.009
5.452 ± 0.03
5.144 ± 0.036
3.265 ± 0.024
9.222 ± 0.038
2.173 ± 0.02
4.394 ± 0.031
2.781 ± 0.028
10.257 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.149 ± 0.017
2.605 ± 0.023
5.412 ± 0.029
3.073 ± 0.018
6.333 ± 0.038
5.866 ± 0.025
5.989 ± 0.031
8.427 ± 0.033
1.454 ± 0.015
2.043 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here