Amino acid dipepetide frequency for Mycobacterium phage Bromden

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.276AlaAla: 12.276 ± 0.765
0.797AlaCys: 0.797 ± 0.193
6.981AlaAsp: 6.981 ± 0.767
6.185AlaGlu: 6.185 ± 0.648
3.608AlaPhe: 3.608 ± 0.403
7.825AlaGly: 7.825 ± 0.527
1.78AlaHis: 1.78 ± 0.34
3.936AlaIle: 3.936 ± 0.458
6.513AlaLys: 6.513 ± 0.64
9.605AlaLeu: 9.605 ± 0.543
3.045AlaMet: 3.045 ± 0.316
3.608AlaAsn: 3.608 ± 0.535
4.029AlaPro: 4.029 ± 0.492
4.357AlaGln: 4.357 ± 0.541
5.248AlaArg: 5.248 ± 0.512
5.435AlaSer: 5.435 ± 0.847
5.669AlaThr: 5.669 ± 0.451
7.309AlaVal: 7.309 ± 0.687
1.78AlaTrp: 1.78 ± 0.33
2.718AlaTyr: 2.718 ± 0.328
0.0AlaXaa: 0.0 ± 0.0
Cys
0.703CysAla: 0.703 ± 0.207
0.375CysCys: 0.375 ± 0.135
0.562CysAsp: 0.562 ± 0.192
0.656CysGlu: 0.656 ± 0.231
0.281CysPhe: 0.281 ± 0.105
1.312CysGly: 1.312 ± 0.305
0.375CysHis: 0.375 ± 0.138
0.328CysIle: 0.328 ± 0.145
0.609CysLys: 0.609 ± 0.196
0.89CysLeu: 0.89 ± 0.217
0.328CysMet: 0.328 ± 0.121
0.515CysAsn: 0.515 ± 0.162
0.656CysPro: 0.656 ± 0.184
0.328CysGln: 0.328 ± 0.137
0.984CysArg: 0.984 ± 0.282
0.656CysSer: 0.656 ± 0.179
0.843CysThr: 0.843 ± 0.205
1.078CysVal: 1.078 ± 0.284
0.187CysTrp: 0.187 ± 0.098
0.328CysTyr: 0.328 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
6.325AspAla: 6.325 ± 0.611
0.797AspCys: 0.797 ± 0.173
5.248AspAsp: 5.248 ± 0.692
4.732AspGlu: 4.732 ± 0.544
2.436AspPhe: 2.436 ± 0.306
7.028AspGly: 7.028 ± 0.409
1.265AspHis: 1.265 ± 0.266
2.858AspIle: 2.858 ± 0.408
3.092AspLys: 3.092 ± 0.417
5.857AspLeu: 5.857 ± 0.546
1.546AspMet: 1.546 ± 0.32
2.202AspAsn: 2.202 ± 0.393
3.889AspPro: 3.889 ± 0.505
1.874AspGln: 1.874 ± 0.338
3.28AspArg: 3.28 ± 0.441
3.701AspSer: 3.701 ± 0.421
2.811AspThr: 2.811 ± 0.323
4.17AspVal: 4.17 ± 0.463
1.874AspTrp: 1.874 ± 0.358
2.718AspTyr: 2.718 ± 0.385
0.0AspXaa: 0.0 ± 0.0
Glu
6.372GluAla: 6.372 ± 0.583
0.656GluCys: 0.656 ± 0.227
3.139GluAsp: 3.139 ± 0.421
1.968GluGlu: 1.968 ± 0.376
2.343GluPhe: 2.343 ± 0.284
3.28GluGly: 3.28 ± 0.343
1.218GluHis: 1.218 ± 0.215
2.202GluIle: 2.202 ± 0.313
1.921GluLys: 1.921 ± 0.275
7.028GluLeu: 7.028 ± 0.627
1.64GluMet: 1.64 ± 0.274
1.499GluAsn: 1.499 ± 0.291
2.53GluPro: 2.53 ± 0.442
2.577GluGln: 2.577 ± 0.361
4.404GluArg: 4.404 ± 0.574
3.092GluSer: 3.092 ± 0.326
2.624GluThr: 2.624 ± 0.346
3.795GluVal: 3.795 ± 0.482
1.171GluTrp: 1.171 ± 0.229
2.39GluTyr: 2.39 ± 0.343
0.0GluXaa: 0.0 ± 0.0
Phe
2.952PheAla: 2.952 ± 0.35
0.281PheCys: 0.281 ± 0.1
2.296PheAsp: 2.296 ± 0.295
1.874PheGlu: 1.874 ± 0.25
1.265PhePhe: 1.265 ± 0.19
3.139PheGly: 3.139 ± 0.344
0.609PheHis: 0.609 ± 0.149
1.593PheIle: 1.593 ± 0.234
1.593PheLys: 1.593 ± 0.273
2.249PheLeu: 2.249 ± 0.351
0.703PheMet: 0.703 ± 0.19
1.452PheAsn: 1.452 ± 0.223
1.827PhePro: 1.827 ± 0.315
0.609PheGln: 0.609 ± 0.172
1.921PheArg: 1.921 ± 0.328
1.968PheSer: 1.968 ± 0.269
1.687PheThr: 1.687 ± 0.27
2.249PheVal: 2.249 ± 0.305
0.703PheTrp: 0.703 ± 0.189
1.64PheTyr: 1.64 ± 0.301
0.0PheXaa: 0.0 ± 0.0
Gly
7.356GlyAla: 7.356 ± 0.871
1.218GlyCys: 1.218 ± 0.349
4.779GlyAsp: 4.779 ± 0.439
5.107GlyGlu: 5.107 ± 0.447
3.139GlyPhe: 3.139 ± 0.437
8.199GlyGly: 8.199 ± 1.221
1.78GlyHis: 1.78 ± 0.327
3.514GlyIle: 3.514 ± 0.35
4.732GlyLys: 4.732 ± 0.601
7.684GlyLeu: 7.684 ± 0.925
2.015GlyMet: 2.015 ± 0.319
2.952GlyAsn: 2.952 ± 0.377
3.233GlyPro: 3.233 ± 0.423
3.327GlyGln: 3.327 ± 0.412
4.404GlyArg: 4.404 ± 0.375
5.107GlySer: 5.107 ± 0.68
4.779GlyThr: 4.779 ± 0.636
6.606GlyVal: 6.606 ± 0.549
2.015GlyTrp: 2.015 ± 0.328
2.905GlyTyr: 2.905 ± 0.368
0.0GlyXaa: 0.0 ± 0.0
His
1.312HisAla: 1.312 ± 0.256
0.187HisCys: 0.187 ± 0.096
1.218HisAsp: 1.218 ± 0.236
0.984HisGlu: 0.984 ± 0.21
0.797HisPhe: 0.797 ± 0.195
1.827HisGly: 1.827 ± 0.285
0.375HisHis: 0.375 ± 0.156
0.937HisIle: 0.937 ± 0.229
0.797HisLys: 0.797 ± 0.183
1.499HisLeu: 1.499 ± 0.265
0.375HisMet: 0.375 ± 0.135
0.609HisAsn: 0.609 ± 0.171
0.937HisPro: 0.937 ± 0.227
0.609HisGln: 0.609 ± 0.168
1.265HisArg: 1.265 ± 0.274
0.797HisSer: 0.797 ± 0.17
0.937HisThr: 0.937 ± 0.202
1.687HisVal: 1.687 ± 0.29
0.515HisTrp: 0.515 ± 0.168
0.843HisTyr: 0.843 ± 0.208
0.0HisXaa: 0.0 ± 0.0
Ile
4.217IleAla: 4.217 ± 0.509
0.469IleCys: 0.469 ± 0.173
2.436IleAsp: 2.436 ± 0.37
2.343IleGlu: 2.343 ± 0.37
1.078IlePhe: 1.078 ± 0.184
3.373IleGly: 3.373 ± 0.41
0.75IleHis: 0.75 ± 0.223
1.593IleIle: 1.593 ± 0.287
1.546IleLys: 1.546 ± 0.28
3.045IleLeu: 3.045 ± 0.368
0.562IleMet: 0.562 ± 0.155
1.968IleAsn: 1.968 ± 0.282
2.202IlePro: 2.202 ± 0.316
1.593IleGln: 1.593 ± 0.245
2.718IleArg: 2.718 ± 0.367
2.296IleSer: 2.296 ± 0.35
2.671IleThr: 2.671 ± 0.365
2.577IleVal: 2.577 ± 0.334
0.703IleTrp: 0.703 ± 0.202
0.515IleTyr: 0.515 ± 0.118
0.0IleXaa: 0.0 ± 0.0
Lys
6.747LysAla: 6.747 ± 0.623
0.75LysCys: 0.75 ± 0.199
3.045LysAsp: 3.045 ± 0.346
2.436LysGlu: 2.436 ± 0.382
1.312LysPhe: 1.312 ± 0.237
3.983LysGly: 3.983 ± 0.61
0.937LysHis: 0.937 ± 0.221
1.359LysIle: 1.359 ± 0.278
3.42LysLys: 3.42 ± 0.449
4.264LysLeu: 4.264 ± 0.46
0.984LysMet: 0.984 ± 0.247
1.874LysAsn: 1.874 ± 0.301
3.467LysPro: 3.467 ± 0.431
2.249LysGln: 2.249 ± 0.375
3.842LysArg: 3.842 ± 0.484
2.952LysSer: 2.952 ± 0.463
1.827LysThr: 1.827 ± 0.327
3.42LysVal: 3.42 ± 0.433
1.312LysTrp: 1.312 ± 0.28
1.64LysTyr: 1.64 ± 0.304
0.0LysXaa: 0.0 ± 0.0
Leu
8.106LeuAla: 8.106 ± 0.555
0.937LeuCys: 0.937 ± 0.195
6.325LeuAsp: 6.325 ± 0.496
4.264LeuGlu: 4.264 ± 0.509
2.53LeuPhe: 2.53 ± 0.444
5.482LeuGly: 5.482 ± 0.491
1.499LeuHis: 1.499 ± 0.294
3.608LeuIle: 3.608 ± 0.406
4.92LeuLys: 4.92 ± 0.52
7.028LeuLeu: 7.028 ± 0.544
2.436LeuMet: 2.436 ± 0.384
3.983LeuAsn: 3.983 ± 0.617
5.576LeuPro: 5.576 ± 0.721
2.577LeuGln: 2.577 ± 0.296
5.013LeuArg: 5.013 ± 0.639
5.904LeuSer: 5.904 ± 0.594
5.997LeuThr: 5.997 ± 0.641
5.576LeuVal: 5.576 ± 0.481
2.062LeuTrp: 2.062 ± 0.332
1.921LeuTyr: 1.921 ± 0.31
0.0LeuXaa: 0.0 ± 0.0
Met
2.905MetAla: 2.905 ± 0.347
0.609MetCys: 0.609 ± 0.19
1.171MetAsp: 1.171 ± 0.232
0.89MetGlu: 0.89 ± 0.185
0.703MetPhe: 0.703 ± 0.193
1.546MetGly: 1.546 ± 0.265
0.562MetHis: 0.562 ± 0.18
0.703MetIle: 0.703 ± 0.168
0.797MetLys: 0.797 ± 0.204
1.78MetLeu: 1.78 ± 0.382
0.281MetMet: 0.281 ± 0.112
0.609MetAsn: 0.609 ± 0.164
1.78MetPro: 1.78 ± 0.313
0.89MetGln: 0.89 ± 0.191
1.312MetArg: 1.312 ± 0.238
2.249MetSer: 2.249 ± 0.305
2.296MetThr: 2.296 ± 0.314
1.406MetVal: 1.406 ± 0.256
0.469MetTrp: 0.469 ± 0.143
0.797MetTyr: 0.797 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
3.842AsnAla: 3.842 ± 0.369
0.328AsnCys: 0.328 ± 0.105
2.155AsnAsp: 2.155 ± 0.327
1.827AsnGlu: 1.827 ± 0.278
0.75AsnPhe: 0.75 ± 0.171
4.123AsnGly: 4.123 ± 0.61
0.609AsnHis: 0.609 ± 0.18
0.89AsnIle: 0.89 ± 0.171
1.593AsnLys: 1.593 ± 0.258
3.608AsnLeu: 3.608 ± 0.421
0.89AsnMet: 0.89 ± 0.214
1.452AsnAsn: 1.452 ± 0.316
2.858AsnPro: 2.858 ± 0.408
1.078AsnGln: 1.078 ± 0.218
2.436AsnArg: 2.436 ± 0.401
1.593AsnSer: 1.593 ± 0.218
2.062AsnThr: 2.062 ± 0.306
2.858AsnVal: 2.858 ± 0.385
0.89AsnTrp: 0.89 ± 0.194
0.937AsnTyr: 0.937 ± 0.183
0.0AsnXaa: 0.0 ± 0.0
Pro
4.779ProAla: 4.779 ± 0.482
0.562ProCys: 0.562 ± 0.227
4.545ProAsp: 4.545 ± 0.437
4.076ProGlu: 4.076 ± 0.497
1.312ProPhe: 1.312 ± 0.268
5.716ProGly: 5.716 ± 0.741
0.656ProHis: 0.656 ± 0.183
1.921ProIle: 1.921 ± 0.268
2.811ProLys: 2.811 ± 0.489
3.373ProLeu: 3.373 ± 0.43
1.312ProMet: 1.312 ± 0.241
2.249ProAsn: 2.249 ± 0.287
3.139ProPro: 3.139 ± 0.359
1.499ProGln: 1.499 ± 0.351
2.952ProArg: 2.952 ± 0.419
2.202ProSer: 2.202 ± 0.347
3.092ProThr: 3.092 ± 0.437
4.404ProVal: 4.404 ± 0.42
1.171ProTrp: 1.171 ± 0.212
1.218ProTyr: 1.218 ± 0.207
0.0ProXaa: 0.0 ± 0.0
Gln
4.592GlnAla: 4.592 ± 0.532
0.656GlnCys: 0.656 ± 0.167
1.499GlnAsp: 1.499 ± 0.238
1.452GlnGlu: 1.452 ± 0.289
1.359GlnPhe: 1.359 ± 0.206
2.718GlnGly: 2.718 ± 0.316
0.75GlnHis: 0.75 ± 0.224
1.218GlnIle: 1.218 ± 0.225
1.827GlnLys: 1.827 ± 0.294
3.233GlnLeu: 3.233 ± 0.455
0.797GlnMet: 0.797 ± 0.184
1.124GlnAsn: 1.124 ± 0.263
1.687GlnPro: 1.687 ± 0.242
1.827GlnGln: 1.827 ± 0.493
2.624GlnArg: 2.624 ± 0.309
1.687GlnSer: 1.687 ± 0.279
2.015GlnThr: 2.015 ± 0.382
2.858GlnVal: 2.858 ± 0.502
0.89GlnTrp: 0.89 ± 0.164
1.124GlnTyr: 1.124 ± 0.213
0.0GlnXaa: 0.0 ± 0.0
Arg
5.716ArgAla: 5.716 ± 0.511
0.89ArgCys: 0.89 ± 0.24
4.826ArgAsp: 4.826 ± 0.515
4.264ArgGlu: 4.264 ± 0.562
1.78ArgPhe: 1.78 ± 0.302
4.592ArgGly: 4.592 ± 0.473
0.937ArgHis: 0.937 ± 0.236
2.671ArgIle: 2.671 ± 0.404
3.842ArgLys: 3.842 ± 0.508
4.545ArgLeu: 4.545 ± 0.432
1.546ArgMet: 1.546 ± 0.304
2.249ArgAsn: 2.249 ± 0.261
2.155ArgPro: 2.155 ± 0.429
1.874ArgGln: 1.874 ± 0.296
4.357ArgArg: 4.357 ± 0.618
3.233ArgSer: 3.233 ± 0.349
2.858ArgThr: 2.858 ± 0.426
5.013ArgVal: 5.013 ± 0.48
1.452ArgTrp: 1.452 ± 0.291
1.827ArgTyr: 1.827 ± 0.333
0.0ArgXaa: 0.0 ± 0.0
Ser
5.716SerAla: 5.716 ± 0.514
0.234SerCys: 0.234 ± 0.123
3.889SerAsp: 3.889 ± 0.389
3.186SerGlu: 3.186 ± 0.388
1.546SerPhe: 1.546 ± 0.315
5.81SerGly: 5.81 ± 0.614
1.031SerHis: 1.031 ± 0.255
2.015SerIle: 2.015 ± 0.343
2.952SerLys: 2.952 ± 0.381
4.639SerLeu: 4.639 ± 0.538
1.078SerMet: 1.078 ± 0.268
2.155SerAsn: 2.155 ± 0.363
2.53SerPro: 2.53 ± 0.314
2.436SerGln: 2.436 ± 0.411
3.373SerArg: 3.373 ± 0.487
3.045SerSer: 3.045 ± 0.479
2.905SerThr: 2.905 ± 0.38
4.966SerVal: 4.966 ± 0.495
1.452SerTrp: 1.452 ± 0.289
1.452SerTyr: 1.452 ± 0.221
0.0SerXaa: 0.0 ± 0.0
Thr
6.091ThrAla: 6.091 ± 0.64
0.656ThrCys: 0.656 ± 0.19
3.983ThrAsp: 3.983 ± 0.448
2.858ThrGlu: 2.858 ± 0.316
2.39ThrPhe: 2.39 ± 0.298
5.107ThrGly: 5.107 ± 0.769
0.75ThrHis: 0.75 ± 0.157
2.53ThrIle: 2.53 ± 0.448
2.624ThrLys: 2.624 ± 0.289
4.92ThrLeu: 4.92 ± 0.495
1.124ThrMet: 1.124 ± 0.241
2.062ThrAsn: 2.062 ± 0.285
3.045ThrPro: 3.045 ± 0.397
1.64ThrGln: 1.64 ± 0.293
2.483ThrArg: 2.483 ± 0.407
2.952ThrSer: 2.952 ± 0.416
3.139ThrThr: 3.139 ± 0.435
5.294ThrVal: 5.294 ± 0.564
1.031ThrTrp: 1.031 ± 0.234
1.499ThrTyr: 1.499 ± 0.308
0.0ThrXaa: 0.0 ± 0.0
Val
7.965ValAla: 7.965 ± 0.687
1.078ValCys: 1.078 ± 0.209
5.763ValAsp: 5.763 ± 0.479
4.076ValGlu: 4.076 ± 0.447
2.015ValPhe: 2.015 ± 0.326
5.622ValGly: 5.622 ± 0.604
1.546ValHis: 1.546 ± 0.305
3.233ValIle: 3.233 ± 0.352
3.889ValLys: 3.889 ± 0.417
5.482ValLeu: 5.482 ± 0.429
1.593ValMet: 1.593 ± 0.264
2.108ValAsn: 2.108 ± 0.3
4.451ValPro: 4.451 ± 0.444
2.671ValGln: 2.671 ± 0.331
4.873ValArg: 4.873 ± 0.481
4.545ValSer: 4.545 ± 0.484
4.732ValThr: 4.732 ± 0.54
6.466ValVal: 6.466 ± 0.817
1.406ValTrp: 1.406 ± 0.275
1.78ValTyr: 1.78 ± 0.352
0.0ValXaa: 0.0 ± 0.0
Trp
1.874TrpAla: 1.874 ± 0.287
0.187TrpCys: 0.187 ± 0.09
1.593TrpAsp: 1.593 ± 0.243
1.124TrpGlu: 1.124 ± 0.257
0.843TrpPhe: 0.843 ± 0.201
1.499TrpGly: 1.499 ± 0.263
0.422TrpHis: 0.422 ± 0.14
0.797TrpIle: 0.797 ± 0.171
1.031TrpLys: 1.031 ± 0.185
2.249TrpLeu: 2.249 ± 0.298
0.703TrpMet: 0.703 ± 0.221
1.031TrpAsn: 1.031 ± 0.233
1.171TrpPro: 1.171 ± 0.213
0.797TrpGln: 0.797 ± 0.21
1.499TrpArg: 1.499 ± 0.353
1.265TrpSer: 1.265 ± 0.273
1.546TrpThr: 1.546 ± 0.305
1.546TrpVal: 1.546 ± 0.265
0.562TrpTrp: 0.562 ± 0.21
0.469TrpTyr: 0.469 ± 0.15
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.905TyrAla: 2.905 ± 0.41
0.328TyrCys: 0.328 ± 0.159
2.249TyrAsp: 2.249 ± 0.346
1.546TyrGlu: 1.546 ± 0.315
1.124TyrPhe: 1.124 ± 0.237
2.671TyrGly: 2.671 ± 0.316
0.656TyrHis: 0.656 ± 0.189
0.75TyrIle: 0.75 ± 0.188
1.312TyrLys: 1.312 ± 0.226
2.764TyrLeu: 2.764 ± 0.385
0.75TyrMet: 0.75 ± 0.192
1.078TyrAsn: 1.078 ± 0.19
1.827TyrPro: 1.827 ± 0.265
1.124TyrGln: 1.124 ± 0.223
1.64TyrArg: 1.64 ± 0.285
1.734TyrSer: 1.734 ± 0.31
1.687TyrThr: 1.687 ± 0.315
2.015TyrVal: 2.015 ± 0.327
0.562TyrTrp: 0.562 ± 0.171
0.656TyrTyr: 0.656 ± 0.158
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 118 proteins (21344 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski