Cytophagaceae bacterium FSY-15

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; unclassified Cytophagaceae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2320 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A437PW34|A0A437PW34_9BACT SDR family NAD(P)-dependent oxidoreductase OS=Cytophagaceae bacterium FSY-15 OX=1862386 GN=EOJ36_00295 PE=3 SV=1
II1 pKa = 6.87ATVKK5 pKa = 10.41SGSSVTTNVLANDD18 pKa = 3.16VCEE21 pKa = 4.6NPNCTLSNPTIVTQPAHH38 pKa = 5.49GTVTVNPDD46 pKa = 2.68GTLTYY51 pKa = 10.04TPAAGFTGTDD61 pKa = 2.91VLTYY65 pKa = 7.88QVCDD69 pKa = 3.46NSVNPAVCKK78 pKa = 9.27TATVTYY84 pKa = 8.42TVQPATAAPTTTAADD99 pKa = 3.9DD100 pKa = 4.09ASTTNSNSPVSGNVLANDD118 pKa = 4.12GSTNPNAVLTVAPQNTTIPGKK139 pKa = 9.35GTIVMNADD147 pKa = 3.14GSFTFTPTGGFVGTVDD163 pKa = 3.99IPYY166 pKa = 8.67TVCDD170 pKa = 3.47NANPANCSTATLHH183 pKa = 6.73IVVEE187 pKa = 4.17PAPTVIAPDD196 pKa = 4.1FNNGLINKK204 pKa = 8.21PIAGSLATNDD214 pKa = 3.32VVAGGGTYY222 pKa = 7.65GTPVAATSNPTGATFTVNPDD242 pKa = 2.68GSYY245 pKa = 10.93SFTGTQPGVYY255 pKa = 8.66TYY257 pKa = 10.79NVPVCPAGQTTNCPLSPVTITVVDD281 pKa = 4.1PTAANVAPAVNPDD294 pKa = 2.88IATVKK299 pKa = 10.25SGSSVTTNVLANDD312 pKa = 3.16VCEE315 pKa = 4.6NPNCTLSNPTIVTQPAHH332 pKa = 5.49GTVTVNPDD340 pKa = 2.68GTLTYY345 pKa = 10.04TPAAGFTGTDD355 pKa = 2.91VLTYY359 pKa = 7.88QVCDD363 pKa = 3.46NSVNPAVCKK372 pKa = 9.92STTVTYY378 pKa = 9.08TVQPATAAPTTTAADD393 pKa = 3.79DD394 pKa = 3.93AASTKK399 pKa = 10.79GNVPATGNVLRR410 pKa = 11.84NDD412 pKa = 3.47ASTDD416 pKa = 3.45PNAVLTVTPQSTTIPGKK433 pKa = 9.33GTIVMNADD441 pKa = 2.99GSYY444 pKa = 9.39TFTPAPGFTGTVNIPYY460 pKa = 7.87TVCDD464 pKa = 3.41NANPANCSTATLHH477 pKa = 6.47VVVEE481 pKa = 4.32PPVDD485 pKa = 3.68LVGDD489 pKa = 4.16INATNINVPVNGNVSTNDD507 pKa = 3.4SAPGSVVYY515 pKa = 7.49GTPVASANNPSGATLTMNPDD535 pKa = 2.89GTYY538 pKa = 10.71SFTATTPGKK547 pKa = 10.31YY548 pKa = 10.27VYY550 pKa = 10.12DD551 pKa = 4.01VPNCLPGQTTGCPTTPLVITVKK573 pKa = 10.85DD574 pKa = 3.91PSATNNLPVVNPDD587 pKa = 2.8IATVRR592 pKa = 11.84AGTSVTTNVLANDD605 pKa = 3.07KK606 pKa = 9.13CTNQGCSLNPASVAITAQPAHH627 pKa = 6.01GTVTVNPDD635 pKa = 2.68GTLTYY640 pKa = 10.04TPAAGFTGTDD650 pKa = 2.87VLTYY654 pKa = 9.78KK655 pKa = 10.49VCDD658 pKa = 3.29NMTPANCQTATVTYY672 pKa = 8.71TVVPANSVLTTAADD686 pKa = 3.95DD687 pKa = 4.0YY688 pKa = 10.9VTTKK692 pKa = 9.69GTGIATGNVLGNDD705 pKa = 3.31ASTNSAATLHH715 pKa = 5.94VSATAAVPASTGTLVMNANGSYY737 pKa = 10.65VFTPASGFTGTLDD750 pKa = 3.36VAYY753 pKa = 8.0TVCDD757 pKa = 3.09NATPANCTEE766 pKa = 4.22ATLHH770 pKa = 5.74ITVDD774 pKa = 3.87PAPTATDD781 pKa = 3.78DD782 pKa = 4.73NIPGATNGVYY792 pKa = 7.25TTNILVNDD800 pKa = 4.36QIQPGAGISITRR812 pKa = 11.84QTGAGAGTAQGTVTFDD828 pKa = 3.75PLTGAMVYY836 pKa = 10.23VPSPLDD842 pKa = 3.7GNTVTVGYY850 pKa = 6.98TVCDD854 pKa = 3.58NNYY857 pKa = 10.21NPAQCADD864 pKa = 3.5ATVTIQVCDD873 pKa = 3.73PANPAMDD880 pKa = 4.65CDD882 pKa = 4.37GDD884 pKa = 4.18GVTNGQEE891 pKa = 4.35ILDD894 pKa = 4.18HH895 pKa = 6.89TDD897 pKa = 3.28PADD900 pKa = 3.88PCSLVAGSQTVAPKK914 pKa = 10.62GIWLTADD921 pKa = 3.46CDD923 pKa = 4.23GDD925 pKa = 4.07GVTNGQEE932 pKa = 4.43VIDD935 pKa = 4.27GTNPTDD941 pKa = 3.58PCDD944 pKa = 3.77YY945 pKa = 10.82NVASKK950 pKa = 9.26TLAPSAAWLALDD962 pKa = 4.59CDD964 pKa = 4.67GDD966 pKa = 4.06GTPNATDD973 pKa = 3.67PQPLNFCVGGTGAIPALGSATYY995 pKa = 11.04DD996 pKa = 3.22KK997 pKa = 10.88FFRR1000 pKa = 11.84NADD1003 pKa = 3.53CDD1005 pKa = 3.8GDD1007 pKa = 4.43GISNHH1012 pKa = 5.78MEE1014 pKa = 4.05TNTSGLALDD1023 pKa = 4.71FDD1025 pKa = 5.3GDD1027 pKa = 4.59GIPNYY1032 pKa = 10.67LDD1034 pKa = 3.98GDD1036 pKa = 4.12SDD1038 pKa = 4.37NDD1040 pKa = 4.68GIPDD1044 pKa = 3.42AVEE1047 pKa = 3.67QNRR1050 pKa = 11.84DD1051 pKa = 3.24SDD1053 pKa = 3.85GDD1055 pKa = 4.05GYY1057 pKa = 11.71ADD1059 pKa = 3.88YY1060 pKa = 11.33QDD1062 pKa = 5.3LDD1064 pKa = 3.89SDD1066 pKa = 4.06NDD1068 pKa = 4.66GISDD1072 pKa = 3.72KK1073 pKa = 11.29VEE1075 pKa = 4.42AGASPTMPVDD1085 pKa = 3.28SDD1087 pKa = 4.19GDD1089 pKa = 3.88GKK1091 pKa = 10.08PNYY1094 pKa = 10.03LDD1096 pKa = 5.17LDD1098 pKa = 3.91SDD1100 pKa = 3.87NDD1102 pKa = 4.66GILDD1106 pKa = 3.77SVEE1109 pKa = 4.68AIDD1112 pKa = 4.67VFAANRR1118 pKa = 11.84DD1119 pKa = 3.62DD1120 pKa = 5.2NYY1122 pKa = 11.27DD1123 pKa = 3.46GKK1125 pKa = 11.14VDD1127 pKa = 3.67KK1128 pKa = 11.36NGVFIDD1134 pKa = 3.81TNNNGWADD1142 pKa = 3.31ISEE1145 pKa = 4.32GDD1147 pKa = 3.99LPVDD1151 pKa = 3.83TDD1153 pKa = 3.55HH1154 pKa = 7.89DD1155 pKa = 5.17GIPDD1159 pKa = 3.88YY1160 pKa = 11.61LDD1162 pKa = 4.7LDD1164 pKa = 4.22SDD1166 pKa = 4.11NDD1168 pKa = 4.43CIPDD1172 pKa = 3.43AVEE1175 pKa = 4.18FTRR1178 pKa = 11.84DD1179 pKa = 2.79VDD1181 pKa = 3.53GDD1183 pKa = 3.47NRR1185 pKa = 11.84PNYY1188 pKa = 9.97RR1189 pKa = 11.84DD1190 pKa = 3.49TDD1192 pKa = 3.74SDD1194 pKa = 4.04GDD1196 pKa = 4.25GIPDD1200 pKa = 3.94NIEE1203 pKa = 4.3AGSCSHH1209 pKa = 7.65PIDD1212 pKa = 3.83TDD1214 pKa = 3.48GDD1216 pKa = 4.37GIPDD1220 pKa = 4.14FLDD1223 pKa = 3.49TDD1225 pKa = 4.07SDD1227 pKa = 3.99NDD1229 pKa = 4.73GIPDD1233 pKa = 4.73AIEE1236 pKa = 4.11AGKK1239 pKa = 10.33NPATPVDD1246 pKa = 3.75TDD1248 pKa = 3.42GDD1250 pKa = 4.5GIPDD1254 pKa = 3.78YY1255 pKa = 11.22RR1256 pKa = 11.84DD1257 pKa = 3.32LDD1259 pKa = 3.89SDD1261 pKa = 3.87NDD1263 pKa = 4.79GIPDD1267 pKa = 3.65ALEE1270 pKa = 4.09AGKK1273 pKa = 10.29DD1274 pKa = 3.42PKK1276 pKa = 11.32NPVDD1280 pKa = 3.62TDD1282 pKa = 3.65GDD1284 pKa = 4.08GTPDD1288 pKa = 3.9FQDD1291 pKa = 3.98LDD1293 pKa = 4.07SDD1295 pKa = 4.01NDD1297 pKa = 4.7GIPDD1301 pKa = 4.74AIEE1304 pKa = 4.07AGKK1307 pKa = 10.36DD1308 pKa = 3.16PKK1310 pKa = 11.32NPVDD1314 pKa = 3.7TDD1316 pKa = 3.5GDD1318 pKa = 4.39GIPDD1322 pKa = 4.01FRR1324 pKa = 11.84DD1325 pKa = 3.17LDD1327 pKa = 3.95SDD1329 pKa = 3.85NDD1331 pKa = 4.79GIPDD1335 pKa = 4.74AIEE1338 pKa = 4.07AGKK1341 pKa = 10.36DD1342 pKa = 3.16PKK1344 pKa = 11.32NPVDD1348 pKa = 3.7TDD1350 pKa = 3.5GDD1352 pKa = 4.39GIPDD1356 pKa = 4.01FRR1358 pKa = 11.84DD1359 pKa = 3.17LDD1361 pKa = 3.95SDD1363 pKa = 3.85NDD1365 pKa = 4.79GIPDD1369 pKa = 3.65ALEE1372 pKa = 4.09AGKK1375 pKa = 10.29DD1376 pKa = 3.42PKK1378 pKa = 11.32NPVDD1382 pKa = 3.7TDD1384 pKa = 3.5GDD1386 pKa = 4.39GIPDD1390 pKa = 3.72FRR1392 pKa = 11.84DD1393 pKa = 2.95VDD1395 pKa = 3.98SDD1397 pKa = 3.65NDD1399 pKa = 4.69GITDD1403 pKa = 3.6AFEE1406 pKa = 5.31AGANPKK1412 pKa = 10.5NPVDD1416 pKa = 3.74TDD1418 pKa = 3.5GDD1420 pKa = 4.39GIPDD1424 pKa = 4.01FRR1426 pKa = 11.84DD1427 pKa = 3.17LDD1429 pKa = 3.85SDD1431 pKa = 3.88NDD1433 pKa = 4.75GISDD1437 pKa = 4.2KK1438 pKa = 11.18IEE1440 pKa = 4.88GIVDD1444 pKa = 3.43TDD1446 pKa = 3.79KK1447 pKa = 11.72DD1448 pKa = 3.93GLPDD1452 pKa = 5.03FRR1454 pKa = 11.84DD1455 pKa = 3.54LDD1457 pKa = 4.28SDD1459 pKa = 3.77GDD1461 pKa = 3.99TYY1463 pKa = 10.97PDD1465 pKa = 3.5KK1466 pKa = 11.73VEE1468 pKa = 4.53GNVDD1472 pKa = 3.37TDD1474 pKa = 3.49KK1475 pKa = 11.74DD1476 pKa = 4.21GIPDD1480 pKa = 4.1FRR1482 pKa = 11.84DD1483 pKa = 3.2LDD1485 pKa = 3.78SDD1487 pKa = 4.17GDD1489 pKa = 4.1VIPDD1493 pKa = 3.75KK1494 pKa = 11.31LEE1496 pKa = 3.67NDD1498 pKa = 2.94IDD1500 pKa = 4.06YY1501 pKa = 11.37GGMPDD1506 pKa = 4.58CDD1508 pKa = 3.94GDD1510 pKa = 4.78GIDD1513 pKa = 5.04NRR1515 pKa = 11.84IDD1517 pKa = 3.77PDD1519 pKa = 3.78VCPTFATQGISPNNDD1534 pKa = 3.56GINDD1538 pKa = 3.51VLIIPGIKK1546 pKa = 9.57SYY1548 pKa = 11.17KK1549 pKa = 8.79NHH1551 pKa = 5.64LTIFNRR1557 pKa = 11.84WGNVVWEE1564 pKa = 4.13QEE1566 pKa = 4.04NYY1568 pKa = 8.04QNSWGGEE1575 pKa = 4.19TNQGDD1580 pKa = 4.31TVLSTDD1586 pKa = 4.13LKK1588 pKa = 11.13LPDD1591 pKa = 3.15GTYY1594 pKa = 10.4YY1595 pKa = 10.92YY1596 pKa = 11.06VIDD1599 pKa = 4.53FYY1601 pKa = 11.33GVKK1604 pKa = 9.98PNIGTYY1610 pKa = 10.5VYY1612 pKa = 10.65INRR1615 pKa = 11.84QAKK1618 pKa = 9.14

Molecular weight: 166.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A437PXL0|A0A437PXL0_9BACT FAD-binding protein OS=Cytophagaceae bacterium FSY-15 OX=1862386 GN=EOJ36_03135 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.59NRR4 pKa = 11.84IFNLGFLVVFLFGITSCWSARR25 pKa = 11.84CPRR28 pKa = 11.84EE29 pKa = 3.74SCRR32 pKa = 11.84VQVEE36 pKa = 4.33HH37 pKa = 6.94RR38 pKa = 11.84HH39 pKa = 5.14GEE41 pKa = 3.96QYY43 pKa = 10.36FRR45 pKa = 11.84PRR47 pKa = 11.84AAFSWMYY54 pKa = 8.28TPRR57 pKa = 11.84YY58 pKa = 8.02KK59 pKa = 10.43HH60 pKa = 5.93IRR62 pKa = 11.84KK63 pKa = 9.46SPIAAGPNQPAVKK76 pKa = 10.45NPWYY80 pKa = 10.36KK81 pKa = 10.33FKK83 pKa = 11.0KK84 pKa = 8.64PSKK87 pKa = 8.99PASKK91 pKa = 10.19PRR93 pKa = 3.43

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2320

0

2320

835184

38

6376

360.0

40.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.843 ± 0.062

0.804 ± 0.017

4.967 ± 0.045

6.273 ± 0.074

5.367 ± 0.057

7.038 ± 0.058

1.796 ± 0.028

7.604 ± 0.057

7.636 ± 0.058

9.653 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.033

5.745 ± 0.062

3.817 ± 0.033

3.912 ± 0.034

3.307 ± 0.035

6.721 ± 0.05

5.255 ± 0.101

6.056 ± 0.036

1.214 ± 0.018

3.713 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski