Amino acid dipepetide frequency for Clostridiaceae bacterium OM08-6BH

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.963AlaAla: 7.963 ± 0.128	1.227AlaCys: 1.227 ± 0.035	4.799AlaAsp: 4.799 ± 0.07	6.155AlaGlu: 6.155 ± 0.097	3.004AlaPhe: 3.004 ± 0.057	6.364AlaGly: 6.364 ± 0.082	1.205AlaHis: 1.205 ± 0.031	4.757AlaIle: 4.757 ± 0.074	4.741AlaLys: 4.741 ± 0.081	6.928AlaLeu: 6.928 ± 0.09	2.46AlaMet: 2.46 ± 0.051	2.484AlaAsn: 2.484 ± 0.049	2.201AlaPro: 2.201 ± 0.048	2.397AlaGln: 2.397 ± 0.054	3.058AlaArg: 3.058 ± 0.066	4.045AlaSer: 4.045 ± 0.071	3.26AlaThr: 3.26 ± 0.07	6.284AlaVal: 6.284 ± 0.081	0.669AlaTrp: 0.669 ± 0.026	2.797AlaTyr: 2.797 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	1.103CysAla: 1.103 ± 0.033	0.286CysCys: 0.286 ± 0.017	0.816CysAsp: 0.816 ± 0.031	0.933CysGlu: 0.933 ± 0.032	0.649CysPhe: 0.649 ± 0.024	1.643CysGly: 1.643 ± 0.047	0.342CysHis: 0.342 ± 0.019	1.066CysIle: 1.066 ± 0.035	0.83CysLys: 0.83 ± 0.028	1.187CysLeu: 1.187 ± 0.032	0.498CysMet: 0.498 ± 0.02	0.526CysAsn: 0.526 ± 0.025	0.695CysPro: 0.695 ± 0.027	0.466CysGln: 0.466 ± 0.019	0.794CysArg: 0.794 ± 0.032	0.902CysSer: 0.902 ± 0.028	0.774CysThr: 0.774 ± 0.031	1.069CysVal: 1.069 ± 0.033	0.152CysTrp: 0.152 ± 0.012	0.565CysTyr: 0.565 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.376AspAla: 4.376 ± 0.07	0.882AspCys: 0.882 ± 0.031	2.961AspAsp: 2.961 ± 0.072	4.578AspGlu: 4.578 ± 0.072	2.601AspPhe: 2.601 ± 0.049	4.674AspGly: 4.674 ± 0.098	0.993AspHis: 0.993 ± 0.033	3.887AspIle: 3.887 ± 0.068	3.173AspLys: 3.173 ± 0.059	4.878AspLeu: 4.878 ± 0.075	1.867AspMet: 1.867 ± 0.045	2.01AspAsn: 2.01 ± 0.047	1.932AspPro: 1.932 ± 0.045	1.782AspGln: 1.782 ± 0.041	2.659AspArg: 2.659 ± 0.076	2.969AspSer: 2.969 ± 0.061	3.132AspThr: 3.132 ± 0.05	3.744AspVal: 3.744 ± 0.071	0.574AspTrp: 0.574 ± 0.026	2.754AspTyr: 2.754 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	5.882GluAla: 5.882 ± 0.084	0.841GluCys: 0.841 ± 0.031	4.368GluAsp: 4.368 ± 0.069	7.656GluGlu: 7.656 ± 0.145	2.507GluPhe: 2.507 ± 0.049	4.359GluGly: 4.359 ± 0.071	1.609GluHis: 1.609 ± 0.045	5.488GluIle: 5.488 ± 0.076	6.737GluLys: 6.737 ± 0.094	7.278GluLeu: 7.278 ± 0.101	2.494GluMet: 2.494 ± 0.056	4.027GluAsn: 4.027 ± 0.069	2.06GluPro: 2.06 ± 0.049	3.646GluGln: 3.646 ± 0.07	3.638GluArg: 3.638 ± 0.075	3.422GluSer: 3.422 ± 0.059	3.989GluThr: 3.989 ± 0.069	4.526GluVal: 4.526 ± 0.07	0.71GluTrp: 0.71 ± 0.026	3.147GluTyr: 3.147 ± 0.057	0.0GluXaa: 0.0 ± 0.0
Phe	2.911PheAla: 2.911 ± 0.053	0.793PheCys: 0.793 ± 0.03	2.423PheAsp: 2.423 ± 0.047	2.482PheGlu: 2.482 ± 0.052	1.719PhePhe: 1.719 ± 0.05	2.919PheGly: 2.919 ± 0.058	0.878PheHis: 0.878 ± 0.031	2.411PheIle: 2.411 ± 0.054	1.777PheLys: 1.777 ± 0.045	4.114PheLeu: 4.114 ± 0.081	1.119PheMet: 1.119 ± 0.034	1.355PheAsn: 1.355 ± 0.036	1.442PhePro: 1.442 ± 0.048	1.404PheGln: 1.404 ± 0.034	1.889PheArg: 1.889 ± 0.041	2.713PheSer: 2.713 ± 0.058	2.332PheThr: 2.332 ± 0.055	2.679PheVal: 2.679 ± 0.058	0.446PheTrp: 0.446 ± 0.021	1.614PheTyr: 1.614 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	5.127GlyAla: 5.127 ± 0.091	1.338GlyCys: 1.338 ± 0.041	3.619GlyAsp: 3.619 ± 0.082	4.712GlyGlu: 4.712 ± 0.071	2.948GlyPhe: 2.948 ± 0.05	4.885GlyGly: 4.885 ± 0.097	1.295GlyHis: 1.295 ± 0.038	5.999GlyIle: 5.999 ± 0.082	5.415GlyLys: 5.415 ± 0.077	6.017GlyLeu: 6.017 ± 0.08	2.553GlyMet: 2.553 ± 0.051	3.081GlyAsn: 3.081 ± 0.056	1.296GlyPro: 1.296 ± 0.034	2.23GlyGln: 2.23 ± 0.048	3.139GlyArg: 3.139 ± 0.059	4.154GlySer: 4.154 ± 0.081	4.639GlyThr: 4.639 ± 0.104	4.911GlyVal: 4.911 ± 0.086	0.754GlyTrp: 0.754 ± 0.025	3.268GlyTyr: 3.268 ± 0.071	0.0GlyXaa: 0.0 ± 0.0
His	1.248HisAla: 1.248 ± 0.036	0.326HisCys: 0.326 ± 0.02	0.931HisAsp: 0.931 ± 0.032	1.228HisGlu: 1.228 ± 0.044	0.882HisPhe: 0.882 ± 0.03	1.385HisGly: 1.385 ± 0.041	0.479HisHis: 0.479 ± 0.028	1.318HisIle: 1.318 ± 0.032	0.929HisLys: 0.929 ± 0.029	1.653HisLeu: 1.653 ± 0.042	0.576HisMet: 0.576 ± 0.024	0.687HisAsn: 0.687 ± 0.027	1.004HisPro: 1.004 ± 0.034	0.619HisGln: 0.619 ± 0.024	0.85HisArg: 0.85 ± 0.029	0.96HisSer: 0.96 ± 0.028	1.068HisThr: 1.068 ± 0.032	1.23HisVal: 1.23 ± 0.035	0.179HisTrp: 0.179 ± 0.012	0.777HisTyr: 0.777 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.353IleAla: 5.353 ± 0.069	1.361IleCys: 1.361 ± 0.036	3.728IleAsp: 3.728 ± 0.057	4.282IleGlu: 4.282 ± 0.071	2.835IlePhe: 2.835 ± 0.049	4.837IleGly: 4.837 ± 0.073	1.399IleHis: 1.399 ± 0.037	4.335IleIle: 4.335 ± 0.078	3.559IleLys: 3.559 ± 0.065	6.748IleLeu: 6.748 ± 0.1	1.842IleMet: 1.842 ± 0.047	2.434IleAsn: 2.434 ± 0.053	3.14IlePro: 3.14 ± 0.063	2.394IleGln: 2.394 ± 0.047	3.857IleArg: 3.857 ± 0.076	4.617IleSer: 4.617 ± 0.074	3.89IleThr: 3.89 ± 0.064	4.618IleVal: 4.618 ± 0.068	0.647IleTrp: 0.647 ± 0.027	2.738IleTyr: 2.738 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	5.109LysAla: 5.109 ± 0.081	0.726LysCys: 0.726 ± 0.03	3.827LysAsp: 3.827 ± 0.053	6.534LysGlu: 6.534 ± 0.103	1.792LysPhe: 1.792 ± 0.043	4.046LysGly: 4.046 ± 0.058	0.972LysHis: 0.972 ± 0.03	4.496LysIle: 4.496 ± 0.074	5.919LysLys: 5.919 ± 0.1	5.414LysLeu: 5.414 ± 0.073	2.169LysMet: 2.169 ± 0.048	3.414LysAsn: 3.414 ± 0.061	2.018LysPro: 2.018 ± 0.053	2.273LysGln: 2.273 ± 0.052	3.031LysArg: 3.031 ± 0.058	3.101LysSer: 3.101 ± 0.057	3.771LysThr: 3.771 ± 0.061	4.167LysVal: 4.167 ± 0.066	0.593LysTrp: 0.593 ± 0.024	2.708LysTyr: 2.708 ± 0.069	0.0LysXaa: 0.0 ± 0.0
Leu	6.902LeuAla: 6.902 ± 0.092	1.53LeuCys: 1.53 ± 0.038	5.161LeuAsp: 5.161 ± 0.075	6.571LeuGlu: 6.571 ± 0.098	3.681LeuPhe: 3.681 ± 0.076	5.869LeuGly: 5.869 ± 0.089	1.679LeuHis: 1.679 ± 0.044	5.8LeuIle: 5.8 ± 0.083	6.358LeuLys: 6.358 ± 0.091	9.02LeuLeu: 9.02 ± 0.145	2.814LeuMet: 2.814 ± 0.051	3.746LeuAsn: 3.746 ± 0.062	3.729LeuPro: 3.729 ± 0.063	3.138LeuGln: 3.138 ± 0.056	3.892LeuArg: 3.892 ± 0.07	6.082LeuSer: 6.082 ± 0.084	5.482LeuThr: 5.482 ± 0.084	5.617LeuVal: 5.617 ± 0.09	0.789LeuTrp: 0.789 ± 0.033	3.443LeuTyr: 3.443 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.567MetAla: 2.567 ± 0.047	0.337MetCys: 0.337 ± 0.019	1.991MetAsp: 1.991 ± 0.042	2.79MetGlu: 2.79 ± 0.047	1.038MetPhe: 1.038 ± 0.032	2.154MetGly: 2.154 ± 0.048	0.477MetHis: 0.477 ± 0.02	2.166MetIle: 2.166 ± 0.049	2.657MetLys: 2.657 ± 0.055	2.797MetLeu: 2.797 ± 0.06	1.063MetMet: 1.063 ± 0.035	1.543MetAsn: 1.543 ± 0.038	1.164MetPro: 1.164 ± 0.038	1.083MetGln: 1.083 ± 0.028	1.266MetArg: 1.266 ± 0.036	1.782MetSer: 1.782 ± 0.045	1.828MetThr: 1.828 ± 0.043	2.033MetVal: 2.033 ± 0.043	0.246MetTrp: 0.246 ± 0.015	0.898MetTyr: 0.898 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	3.027AsnAla: 3.027 ± 0.053	0.648AsnCys: 0.648 ± 0.026	1.964AsnAsp: 1.964 ± 0.046	2.611AsnGlu: 2.611 ± 0.06	1.467AsnPhe: 1.467 ± 0.037	3.474AsnGly: 3.474 ± 0.066	0.814AsnHis: 0.814 ± 0.029	3.017AsnIle: 3.017 ± 0.055	2.112AsnLys: 2.112 ± 0.047	3.694AsnLeu: 3.694 ± 0.062	1.338AsnMet: 1.338 ± 0.041	1.558AsnAsn: 1.558 ± 0.046	1.986AsnPro: 1.986 ± 0.048	1.432AsnGln: 1.432 ± 0.034	2.056AsnArg: 2.056 ± 0.054	2.118AsnSer: 2.118 ± 0.058	2.21AsnThr: 2.21 ± 0.054	2.817AsnVal: 2.817 ± 0.059	0.438AsnTrp: 0.438 ± 0.025	1.818AsnTyr: 1.818 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	2.705ProAla: 2.705 ± 0.059	0.461ProCys: 0.461 ± 0.021	2.449ProAsp: 2.449 ± 0.061	3.794ProGlu: 3.794 ± 0.075	1.556ProPhe: 1.556 ± 0.037	2.393ProGly: 2.393 ± 0.055	0.612ProHis: 0.612 ± 0.025	1.981ProIle: 1.981 ± 0.047	1.949ProLys: 1.949 ± 0.041	2.762ProLeu: 2.762 ± 0.056	0.952ProMet: 0.952 ± 0.031	1.231ProAsn: 1.231 ± 0.037	0.81ProPro: 0.81 ± 0.033	1.085ProGln: 1.085 ± 0.037	1.082ProArg: 1.082 ± 0.034	1.774ProSer: 1.774 ± 0.05	1.678ProThr: 1.678 ± 0.046	3.174ProVal: 3.174 ± 0.056	0.358ProTrp: 0.358 ± 0.019	1.453ProTyr: 1.453 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	2.72GlnAla: 2.72 ± 0.057	0.356GlnCys: 0.356 ± 0.017	1.664GlnAsp: 1.664 ± 0.041	3.062GlnGlu: 3.062 ± 0.069	1.161GlnPhe: 1.161 ± 0.03	2.023GlnGly: 2.023 ± 0.054	0.544GlnHis: 0.544 ± 0.023	2.747GlnIle: 2.747 ± 0.052	2.922GlnLys: 2.922 ± 0.048	3.019GlnLeu: 3.019 ± 0.061	1.316GlnMet: 1.316 ± 0.034	1.716GlnAsn: 1.716 ± 0.046	1.052GlnPro: 1.052 ± 0.033	1.365GlnGln: 1.365 ± 0.043	1.474GlnArg: 1.474 ± 0.039	1.67GlnSer: 1.67 ± 0.041	1.892GlnThr: 1.892 ± 0.048	2.252GlnVal: 2.252 ± 0.046	0.335GlnTrp: 0.335 ± 0.017	1.501GlnTyr: 1.501 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.814ArgAla: 2.814 ± 0.054	0.654ArgCys: 0.654 ± 0.024	2.112ArgAsp: 2.112 ± 0.042	3.972ArgGlu: 3.972 ± 0.086	1.822ArgPhe: 1.822 ± 0.048	2.476ArgGly: 2.476 ± 0.054	0.864ArgHis: 0.864 ± 0.031	3.431ArgIle: 3.431 ± 0.066	3.801ArgLys: 3.801 ± 0.07	4.172ArgLeu: 4.172 ± 0.079	1.764ArgMet: 1.764 ± 0.046	2.027ArgAsn: 2.027 ± 0.049	1.461ArgPro: 1.461 ± 0.047	1.96ArgGln: 1.96 ± 0.051	2.457ArgArg: 2.457 ± 0.054	2.321ArgSer: 2.321 ± 0.048	2.401ArgThr: 2.401 ± 0.049	2.744ArgVal: 2.744 ± 0.05	0.407ArgTrp: 0.407 ± 0.022	1.956ArgTyr: 1.956 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	4.143SerAla: 4.143 ± 0.075	0.847SerCys: 0.847 ± 0.028	3.288SerAsp: 3.288 ± 0.061	4.06SerGlu: 4.06 ± 0.078	2.524SerPhe: 2.524 ± 0.051	5.01SerGly: 5.01 ± 0.093	0.999SerHis: 0.999 ± 0.034	3.867SerIle: 3.867 ± 0.056	2.883SerLys: 2.883 ± 0.055	4.945SerLeu: 4.945 ± 0.076	1.963SerMet: 1.963 ± 0.045	2.05SerAsn: 2.05 ± 0.054	1.68SerPro: 1.68 ± 0.044	1.837SerGln: 1.837 ± 0.042	2.844SerArg: 2.844 ± 0.06	3.467SerSer: 3.467 ± 0.079	2.812SerThr: 2.812 ± 0.057	4.126SerVal: 4.126 ± 0.065	0.587SerTrp: 0.587 ± 0.026	2.41SerTyr: 2.41 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	4.788ThrAla: 4.788 ± 0.093	0.66ThrCys: 0.66 ± 0.028	3.458ThrAsp: 3.458 ± 0.07	4.238ThrGlu: 4.238 ± 0.072	2.17ThrPhe: 2.17 ± 0.051	4.898ThrGly: 4.898 ± 0.081	0.913ThrHis: 0.913 ± 0.03	3.698ThrIle: 3.698 ± 0.073	2.899ThrLys: 2.899 ± 0.049	4.866ThrLeu: 4.866 ± 0.082	1.546ThrMet: 1.546 ± 0.035	1.822ThrAsn: 1.822 ± 0.046	2.269ThrPro: 2.269 ± 0.056	1.469ThrGln: 1.469 ± 0.038	2.087ThrArg: 2.087 ± 0.046	3.046ThrSer: 3.046 ± 0.066	2.886ThrThr: 2.886 ± 0.081	4.784ThrVal: 4.784 ± 0.095	0.528ThrTrp: 0.528 ± 0.025	2.205ThrTyr: 2.205 ± 0.057	0.0ThrXaa: 0.0 ± 0.0
Val	4.687ValAla: 4.687 ± 0.071	1.26ValCys: 1.26 ± 0.039	3.776ValAsp: 3.776 ± 0.065	4.81ValGlu: 4.81 ± 0.077	2.87ValPhe: 2.87 ± 0.059	4.281ValGly: 4.281 ± 0.067	1.173ValHis: 1.173 ± 0.034	4.842ValIle: 4.842 ± 0.071	4.433ValLys: 4.433 ± 0.074	7.014ValLeu: 7.014 ± 0.096	2.074ValMet: 2.074 ± 0.043	2.699ValAsn: 2.699 ± 0.049	2.617ValPro: 2.617 ± 0.051	2.256ValGln: 2.256 ± 0.048	3.051ValArg: 3.051 ± 0.051	4.517ValSer: 4.517 ± 0.077	4.374ValThr: 4.374 ± 0.09	4.697ValVal: 4.697 ± 0.084	0.648ValTrp: 0.648 ± 0.029	2.581ValTyr: 2.581 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.534TrpAla: 0.534 ± 0.024	0.148TrpCys: 0.148 ± 0.015	0.533TrpAsp: 0.533 ± 0.021	0.707TrpGlu: 0.707 ± 0.026	0.457TrpPhe: 0.457 ± 0.021	0.618TrpGly: 0.618 ± 0.024	0.159TrpHis: 0.159 ± 0.011	0.657TrpIle: 0.657 ± 0.028	0.851TrpLys: 0.851 ± 0.029	0.926TrpLeu: 0.926 ± 0.032	0.356TrpMet: 0.356 ± 0.019	0.619TrpAsn: 0.619 ± 0.029	0.217TrpPro: 0.217 ± 0.013	0.383TrpGln: 0.383 ± 0.02	0.393TrpArg: 0.393 ± 0.02	0.485TrpSer: 0.485 ± 0.022	0.453TrpThr: 0.453 ± 0.022	0.491TrpVal: 0.491 ± 0.023	0.093TrpTrp: 0.093 ± 0.009	0.459TrpTyr: 0.459 ± 0.031	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.843TyrAla: 2.843 ± 0.049	0.615TyrCys: 0.615 ± 0.024	2.559TyrAsp: 2.559 ± 0.053	3.208TyrGlu: 3.208 ± 0.06	1.738TyrPhe: 1.738 ± 0.04	3.07TyrGly: 3.07 ± 0.058	0.923TyrHis: 0.923 ± 0.027	2.65TyrIle: 2.65 ± 0.055	2.133TyrLys: 2.133 ± 0.047	3.87TyrLeu: 3.87 ± 0.065	1.116TyrMet: 1.116 ± 0.031	1.569TyrAsn: 1.569 ± 0.046	1.487TyrPro: 1.487 ± 0.039	1.57TyrGln: 1.57 ± 0.035	2.098TyrArg: 2.098 ± 0.045	2.198TyrSer: 2.198 ± 0.055	2.361TyrThr: 2.361 ± 0.069	2.699TyrVal: 2.699 ± 0.054	0.388TyrTrp: 0.388 ± 0.02	1.943TyrTyr: 1.943 ± 0.063	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3400 proteins (1057526 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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