Amino acid dipepetide frequency for Piscinibacter aquaticus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.682AlaAla: 22.682 ± 0.291	1.723AlaCys: 1.723 ± 0.055	6.807AlaAsp: 6.807 ± 0.104	7.447AlaGlu: 7.447 ± 0.129	3.997AlaPhe: 3.997 ± 0.081	12.806AlaGly: 12.806 ± 0.176	2.905AlaHis: 2.905 ± 0.069	4.865AlaIle: 4.865 ± 0.081	3.58AlaLys: 3.58 ± 0.098	15.909AlaLeu: 15.909 ± 0.223	3.758AlaMet: 3.758 ± 0.076	2.544AlaAsn: 2.544 ± 0.059	7.512AlaPro: 7.512 ± 0.143	5.892AlaGln: 5.892 ± 0.107	11.717AlaArg: 11.717 ± 0.166	7.925AlaSer: 7.925 ± 0.118	6.499AlaThr: 6.499 ± 0.109	9.503AlaVal: 9.503 ± 0.113	1.809AlaTrp: 1.809 ± 0.06	2.095AlaTyr: 2.095 ± 0.059	0.0AlaXaa: 0.0 ± 0.0
Cys	1.739CysAla: 1.739 ± 0.054	0.345CysCys: 0.345 ± 0.029	0.566CysAsp: 0.566 ± 0.03	0.544CysGlu: 0.544 ± 0.028	0.272CysPhe: 0.272 ± 0.019	1.139CysGly: 1.139 ± 0.044	0.398CysHis: 0.398 ± 0.05	0.417CysIle: 0.417 ± 0.026	0.186CysLys: 0.186 ± 0.018	0.789CysLeu: 0.789 ± 0.031	0.239CysMet: 0.239 ± 0.021	0.224CysAsn: 0.224 ± 0.017	0.611CysPro: 0.611 ± 0.034	0.207CysGln: 0.207 ± 0.017	1.332CysArg: 1.332 ± 0.059	0.835CysSer: 0.835 ± 0.046	0.593CysThr: 0.593 ± 0.033	0.682CysVal: 0.682 ± 0.029	0.251CysTrp: 0.251 ± 0.022	0.171CysTyr: 0.171 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	7.736AspAla: 7.736 ± 0.125	0.458AspCys: 0.458 ± 0.033	2.904AspAsp: 2.904 ± 0.066	3.271AspGlu: 3.271 ± 0.067	1.777AspPhe: 1.777 ± 0.048	4.894AspGly: 4.894 ± 0.109	1.163AspHis: 1.163 ± 0.049	1.916AspIle: 1.916 ± 0.052	1.302AspLys: 1.302 ± 0.053	4.658AspLeu: 4.658 ± 0.081	0.948AspMet: 0.948 ± 0.037	0.947AspAsn: 0.947 ± 0.04	2.872AspPro: 2.872 ± 0.069	1.26AspGln: 1.26 ± 0.05	3.97AspArg: 3.97 ± 0.087	2.214AspSer: 2.214 ± 0.057	2.368AspThr: 2.368 ± 0.068	3.6AspVal: 3.6 ± 0.091	0.941AspTrp: 0.941 ± 0.038	1.113AspTyr: 1.113 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	7.621GluAla: 7.621 ± 0.123	0.401GluCys: 0.401 ± 0.022	1.886GluAsp: 1.886 ± 0.061	2.116GluGlu: 2.116 ± 0.068	1.609GluPhe: 1.609 ± 0.048	3.766GluGly: 3.766 ± 0.088	1.273GluHis: 1.273 ± 0.04	2.376GluIle: 2.376 ± 0.061	1.547GluLys: 1.547 ± 0.05	6.166GluLeu: 6.166 ± 0.113	1.128GluMet: 1.128 ± 0.042	0.883GluAsn: 0.883 ± 0.034	3.046GluPro: 3.046 ± 0.073	2.373GluGln: 2.373 ± 0.07	5.658GluArg: 5.658 ± 0.113	2.309GluSer: 2.309 ± 0.067	2.308GluThr: 2.308 ± 0.058	3.928GluVal: 3.928 ± 0.087	0.732GluTrp: 0.732 ± 0.033	0.826GluTyr: 0.826 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	4.264PheAla: 4.264 ± 0.091	0.355PheCys: 0.355 ± 0.023	2.249PheAsp: 2.249 ± 0.056	2.061PheGlu: 2.061 ± 0.066	1.184PhePhe: 1.184 ± 0.045	3.173PheGly: 3.173 ± 0.077	0.693PheHis: 0.693 ± 0.036	1.145PheIle: 1.145 ± 0.044	0.903PheLys: 0.903 ± 0.041	2.468PheLeu: 2.468 ± 0.076	0.644PheMet: 0.644 ± 0.029	0.897PheAsn: 0.897 ± 0.04	1.334PhePro: 1.334 ± 0.04	0.871PheGln: 0.871 ± 0.038	2.048PheArg: 2.048 ± 0.056	1.773PheSer: 1.773 ± 0.057	1.667PheThr: 1.667 ± 0.053	2.757PheVal: 2.757 ± 0.071	0.502PheTrp: 0.502 ± 0.027	0.672PheTyr: 0.672 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	10.596GlyAla: 10.596 ± 0.149	1.24GlyCys: 1.24 ± 0.043	4.097GlyAsp: 4.097 ± 0.079	4.675GlyGlu: 4.675 ± 0.089	3.108GlyPhe: 3.108 ± 0.075	7.549GlyGly: 7.549 ± 0.172	2.17GlyHis: 2.17 ± 0.066	3.488GlyIle: 3.488 ± 0.086	2.535GlyLys: 2.535 ± 0.091	8.376GlyLeu: 8.376 ± 0.135	2.11GlyMet: 2.11 ± 0.057	1.859GlyAsn: 1.859 ± 0.057	3.609GlyPro: 3.609 ± 0.082	2.908GlyGln: 2.908 ± 0.065	7.822GlyArg: 7.822 ± 0.148	5.041GlySer: 5.041 ± 0.101	4.633GlyThr: 4.633 ± 0.092	6.163GlyVal: 6.163 ± 0.091	1.753GlyTrp: 1.753 ± 0.056	1.813GlyTyr: 1.813 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	3.271HisAla: 3.271 ± 0.079	0.272HisCys: 0.272 ± 0.022	1.221HisAsp: 1.221 ± 0.05	1.311HisGlu: 1.311 ± 0.049	0.836HisPhe: 0.836 ± 0.041	2.378HisGly: 2.378 ± 0.064	0.64HisHis: 0.64 ± 0.041	0.688HisIle: 0.688 ± 0.034	0.46HisLys: 0.46 ± 0.031	2.19HisLeu: 2.19 ± 0.066	0.419HisMet: 0.419 ± 0.028	0.378HisAsn: 0.378 ± 0.025	1.547HisPro: 1.547 ± 0.044	0.661HisGln: 0.661 ± 0.037	2.361HisArg: 2.361 ± 0.078	0.98HisSer: 0.98 ± 0.044	0.841HisThr: 0.841 ± 0.034	1.52HisVal: 1.52 ± 0.047	0.448HisTrp: 0.448 ± 0.023	0.523HisTyr: 0.523 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.859IleAla: 5.859 ± 0.103	0.348IleCys: 0.348 ± 0.024	2.992IleAsp: 2.992 ± 0.067	2.989IleGlu: 2.989 ± 0.074	1.056IlePhe: 1.056 ± 0.04	3.923IleGly: 3.923 ± 0.087	0.726IleHis: 0.726 ± 0.037	1.007IleIle: 1.007 ± 0.045	1.059IleLys: 1.059 ± 0.047	2.502IleLeu: 2.502 ± 0.067	0.538IleMet: 0.538 ± 0.029	1.015IleAsn: 1.015 ± 0.042	1.617IlePro: 1.617 ± 0.059	0.994IleGln: 0.994 ± 0.039	2.521IleArg: 2.521 ± 0.063	1.925IleSer: 1.925 ± 0.048	2.167IleThr: 2.167 ± 0.063	3.414IleVal: 3.414 ± 0.083	0.392IleTrp: 0.392 ± 0.026	0.678IleTyr: 0.678 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	3.607LysAla: 3.607 ± 0.095	0.166LysCys: 0.166 ± 0.018	1.242LysAsp: 1.242 ± 0.047	1.19LysGlu: 1.19 ± 0.055	0.735LysPhe: 0.735 ± 0.037	2.064LysGly: 2.064 ± 0.073	0.522LysHis: 0.522 ± 0.029	1.047LysIle: 1.047 ± 0.047	1.175LysLys: 1.175 ± 0.053	2.976LysLeu: 2.976 ± 0.073	0.67LysMet: 0.67 ± 0.031	0.718LysAsn: 0.718 ± 0.039	1.881LysPro: 1.881 ± 0.057	0.995LysGln: 0.995 ± 0.041	2.169LysArg: 2.169 ± 0.055	1.435LysSer: 1.435 ± 0.051	1.493LysThr: 1.493 ± 0.055	2.272LysVal: 2.272 ± 0.071	0.277LysTrp: 0.277 ± 0.021	0.516LysTyr: 0.516 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	15.153LeuAla: 15.153 ± 0.202	0.9LeuCys: 0.9 ± 0.037	5.824LeuAsp: 5.824 ± 0.091	5.088LeuGlu: 5.088 ± 0.096	3.005LeuPhe: 3.005 ± 0.072	8.359LeuGly: 8.359 ± 0.131	2.388LeuHis: 2.388 ± 0.069	3.807LeuIle: 3.807 ± 0.087	3.12LeuLys: 3.12 ± 0.079	11.027LeuLeu: 11.027 ± 0.194	2.328LeuMet: 2.328 ± 0.069	2.279LeuAsn: 2.279 ± 0.06	6.328LeuPro: 6.328 ± 0.108	3.928LeuGln: 3.928 ± 0.079	8.922LeuArg: 8.922 ± 0.132	5.61LeuSer: 5.61 ± 0.097	4.834LeuThr: 4.834 ± 0.09	7.134LeuVal: 7.134 ± 0.114	1.41LeuTrp: 1.41 ± 0.047	1.733LeuTyr: 1.733 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	3.161MetAla: 3.161 ± 0.074	0.154MetCys: 0.154 ± 0.015	1.021MetAsp: 1.021 ± 0.042	0.904MetGlu: 0.904 ± 0.039	0.569MetPhe: 0.569 ± 0.029	1.608MetGly: 1.608 ± 0.044	0.485MetHis: 0.485 ± 0.025	0.873MetIle: 0.873 ± 0.031	1.048MetLys: 1.048 ± 0.037	2.571MetLeu: 2.571 ± 0.069	0.452MetMet: 0.452 ± 0.026	0.777MetAsn: 0.777 ± 0.034	1.541MetPro: 1.541 ± 0.05	0.894MetGln: 0.894 ± 0.04	1.96MetArg: 1.96 ± 0.055	1.497MetSer: 1.497 ± 0.05	1.378MetThr: 1.378 ± 0.046	1.611MetVal: 1.611 ± 0.058	0.186MetTrp: 0.186 ± 0.019	0.342MetTyr: 0.342 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.815AsnAla: 2.815 ± 0.067	0.237AsnCys: 0.237 ± 0.022	1.113AsnAsp: 1.113 ± 0.045	1.068AsnGlu: 1.068 ± 0.042	0.726AsnPhe: 0.726 ± 0.031	1.918AsnGly: 1.918 ± 0.07	0.413AsnHis: 0.413 ± 0.027	0.848AsnIle: 0.848 ± 0.038	0.611AsnLys: 0.611 ± 0.034	2.145AsnLeu: 2.145 ± 0.063	0.437AsnMet: 0.437 ± 0.024	0.493AsnAsn: 0.493 ± 0.029	1.49AsnPro: 1.49 ± 0.052	0.668AsnGln: 0.668 ± 0.033	1.568AsnArg: 1.568 ± 0.041	0.942AsnSer: 0.942 ± 0.041	1.116AsnThr: 1.116 ± 0.043	1.661AsnVal: 1.661 ± 0.053	0.331AsnTrp: 0.331 ± 0.024	0.489AsnTyr: 0.489 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	9.398ProAla: 9.398 ± 0.14	0.582ProCys: 0.582 ± 0.031	2.966ProAsp: 2.966 ± 0.074	3.155ProGlu: 3.155 ± 0.067	1.652ProPhe: 1.652 ± 0.048	5.092ProGly: 5.092 ± 0.091	1.218ProHis: 1.218 ± 0.043	1.757ProIle: 1.757 ± 0.051	1.453ProLys: 1.453 ± 0.05	5.386ProLeu: 5.386 ± 0.097	1.473ProMet: 1.473 ± 0.048	1.145ProAsn: 1.145 ± 0.048	3.525ProPro: 3.525 ± 0.096	2.018ProGln: 2.018 ± 0.055	4.686ProArg: 4.686 ± 0.099	3.17ProSer: 3.17 ± 0.074	2.768ProThr: 2.768 ± 0.07	4.45ProVal: 4.45 ± 0.085	0.976ProTrp: 0.976 ± 0.043	0.901ProTyr: 0.901 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	4.748GlnAla: 4.748 ± 0.086	0.371GlnCys: 0.371 ± 0.025	1.208GlnAsp: 1.208 ± 0.039	1.234GlnGlu: 1.234 ± 0.045	1.053GlnPhe: 1.053 ± 0.04	2.91GlnGly: 2.91 ± 0.074	0.889GlnHis: 0.889 ± 0.04	1.546GlnIle: 1.546 ± 0.053	0.817GlnLys: 0.817 ± 0.034	3.949GlnLeu: 3.949 ± 0.08	0.862GlnMet: 0.862 ± 0.04	0.581GlnAsn: 0.581 ± 0.028	2.688GlnPro: 2.688 ± 0.077	1.682GlnGln: 1.682 ± 0.066	4.406GlnArg: 4.406 ± 0.095	1.585GlnSer: 1.585 ± 0.048	1.573GlnThr: 1.573 ± 0.055	2.175GlnVal: 2.175 ± 0.056	0.608GlnTrp: 0.608 ± 0.034	0.576GlnTyr: 0.576 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	11.461ArgAla: 11.461 ± 0.177	1.571ArgCys: 1.571 ± 0.069	4.162ArgAsp: 4.162 ± 0.083	4.962ArgGlu: 4.962 ± 0.093	2.987ArgPhe: 2.987 ± 0.064	6.277ArgGly: 6.277 ± 0.123	2.675ArgHis: 2.675 ± 0.068	3.654ArgIle: 3.654 ± 0.064	1.789ArgLys: 1.789 ± 0.059	8.74ArgLeu: 8.74 ± 0.131	2.067ArgMet: 2.067 ± 0.052	1.508ArgAsn: 1.508 ± 0.042	5.315ArgPro: 5.315 ± 0.12	3.097ArgGln: 3.097 ± 0.073	9.628ArgArg: 9.628 ± 0.214	5.171ArgSer: 5.171 ± 0.114	3.757ArgThr: 3.757 ± 0.069	5.593ArgVal: 5.593 ± 0.092	1.871ArgTrp: 1.871 ± 0.06	1.605ArgTyr: 1.605 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	7.558SerAla: 7.558 ± 0.129	0.679SerCys: 0.679 ± 0.036	2.437SerAsp: 2.437 ± 0.057	2.415SerGlu: 2.415 ± 0.059	1.912SerPhe: 1.912 ± 0.059	5.348SerGly: 5.348 ± 0.092	1.047SerHis: 1.047 ± 0.04	2.069SerIle: 2.069 ± 0.05	1.286SerLys: 1.286 ± 0.047	5.427SerLeu: 5.427 ± 0.091	1.399SerMet: 1.399 ± 0.047	1.136SerAsn: 1.136 ± 0.043	3.265SerPro: 3.265 ± 0.074	1.531SerGln: 1.531 ± 0.056	4.596SerArg: 4.596 ± 0.106	3.633SerSer: 3.633 ± 0.104	3.166SerThr: 3.166 ± 0.085	3.97SerVal: 3.97 ± 0.081	0.827SerTrp: 0.827 ± 0.036	0.971SerTyr: 0.971 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	6.348ThrAla: 6.348 ± 0.111	0.522ThrCys: 0.522 ± 0.034	2.222ThrAsp: 2.222 ± 0.058	2.123ThrGlu: 2.123 ± 0.059	1.443ThrPhe: 1.443 ± 0.048	4.501ThrGly: 4.501 ± 0.078	1.013ThrHis: 1.013 ± 0.041	1.863ThrIle: 1.863 ± 0.056	1.198ThrLys: 1.198 ± 0.043	6.139ThrLeu: 6.139 ± 0.094	1.057ThrMet: 1.057 ± 0.039	1.05ThrAsn: 1.05 ± 0.042	3.533ThrPro: 3.533 ± 0.078	1.559ThrGln: 1.559 ± 0.057	3.71ThrArg: 3.71 ± 0.068	2.812ThrSer: 2.812 ± 0.078	2.819ThrThr: 2.819 ± 0.086	4.223ThrVal: 4.223 ± 0.083	0.492ThrTrp: 0.492 ± 0.027	0.855ThrTyr: 0.855 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	10.08ValAla: 10.08 ± 0.129	0.752ValCys: 0.752 ± 0.034	3.899ValAsp: 3.899 ± 0.071	3.925ValGlu: 3.925 ± 0.083	2.539ValPhe: 2.539 ± 0.053	5.271ValGly: 5.271 ± 0.09	1.594ValHis: 1.594 ± 0.052	3.17ValIle: 3.17 ± 0.076	2.164ValLys: 2.164 ± 0.06	7.987ValLeu: 7.987 ± 0.125	1.767ValMet: 1.767 ± 0.053	1.783ValAsn: 1.783 ± 0.055	4.071ValPro: 4.071 ± 0.081	2.668ValGln: 2.668 ± 0.063	5.48ValArg: 5.48 ± 0.088	3.929ValSer: 3.929 ± 0.089	3.81ValThr: 3.81 ± 0.097	6.03ValVal: 6.03 ± 0.113	1.0ValTrp: 1.0 ± 0.04	1.317ValTyr: 1.317 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.661TrpAla: 1.661 ± 0.051	0.274TrpCys: 0.274 ± 0.019	0.534TrpAsp: 0.534 ± 0.027	0.496TrpGlu: 0.496 ± 0.029	0.508TrpPhe: 0.508 ± 0.025	0.953TrpGly: 0.953 ± 0.042	0.389TrpHis: 0.389 ± 0.024	0.631TrpIle: 0.631 ± 0.032	0.449TrpLys: 0.449 ± 0.029	1.983TrpLeu: 1.983 ± 0.06	0.407TrpMet: 0.407 ± 0.023	0.405TrpAsn: 0.405 ± 0.027	1.048TrpPro: 1.048 ± 0.043	0.681TrpGln: 0.681 ± 0.036	1.744TrpArg: 1.744 ± 0.062	0.93TrpSer: 0.93 ± 0.036	0.696TrpThr: 0.696 ± 0.031	1.095TrpVal: 1.095 ± 0.041	0.38TrpTrp: 0.38 ± 0.028	0.239TrpTyr: 0.239 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.219TyrAla: 2.219 ± 0.052	0.191TyrCys: 0.191 ± 0.017	0.965TyrAsp: 0.965 ± 0.035	0.977TyrGlu: 0.977 ± 0.039	0.691TyrPhe: 0.691 ± 0.038	1.617TyrGly: 1.617 ± 0.05	0.343TyrHis: 0.343 ± 0.019	0.552TyrIle: 0.552 ± 0.03	0.469TyrLys: 0.469 ± 0.03	1.788TyrLeu: 1.788 ± 0.051	0.327TyrMet: 0.327 ± 0.023	0.467TyrAsn: 0.467 ± 0.026	0.936TyrPro: 0.936 ± 0.037	0.573TyrGln: 0.573 ± 0.027	1.594TyrArg: 1.594 ± 0.057	0.953TyrSer: 0.953 ± 0.032	0.997TyrThr: 0.997 ± 0.044	1.458TyrVal: 1.458 ± 0.051	0.318TyrTrp: 0.318 ± 0.02	0.451TyrTyr: 0.451 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2624 proteins (661187 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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