Leptidea sinapis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia;

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19862 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5E4R4H1|A0A5E4R4H1_9NEOP Uncharacterized protein OS=Leptidea sinapis OX=189913 GN=LSINAPIS_LOCUS14834 PE=4 SV=1
MM1 pKa = 7.32SRR3 pKa = 11.84NAKK6 pKa = 10.23AFGEE10 pKa = 4.51GEE12 pKa = 4.14PGDD15 pKa = 3.83VGDD18 pKa = 5.05RR19 pKa = 11.84DD20 pKa = 3.54VDD22 pKa = 3.95GAGPAATSTTTWRR35 pKa = 11.84QALTTDD41 pKa = 3.77GSGFTAKK48 pKa = 10.13PWPCCLDD55 pKa = 3.3ARR57 pKa = 11.84EE58 pKa = 4.25VPLAGQLNHH67 pKa = 6.21EE68 pKa = 4.67LAWGQGWGWVTLPNGVWSLTWSQDD92 pKa = 2.76GDD94 pKa = 4.21DD95 pKa = 4.43FALHH99 pKa = 6.97AMPIMHH105 pKa = 6.81HH106 pKa = 6.02TNVSQACSILDD117 pKa = 3.4GVTAYY122 pKa = 8.47EE123 pKa = 3.89ASAYY127 pKa = 8.61EE128 pKa = 4.02QALAEE133 pKa = 4.32YY134 pKa = 10.05DD135 pKa = 3.8DD136 pKa = 4.23TATTSDD142 pKa = 3.55DD143 pKa = 2.89WDD145 pKa = 4.32FGVDD149 pKa = 3.74CGDD152 pKa = 3.51FMMM155 pKa = 6.18

Molecular weight: 16.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5E4R0L8|A0A5E4R0L8_9NEOP Isoform of A0A5E4R152 Uncharacterized protein OS=Leptidea sinapis OX=189913 GN=LSINAPIS_LOCUS13011 PE=4 SV=1
MM1 pKa = 7.97DD2 pKa = 4.19VDD4 pKa = 3.59TDD6 pKa = 3.48GRR8 pKa = 11.84TSRR11 pKa = 11.84SRR13 pKa = 11.84SRR15 pKa = 11.84SVEE18 pKa = 3.77SKK20 pKa = 9.73MSHH23 pKa = 7.02DD24 pKa = 3.75GSKK27 pKa = 10.31SRR29 pKa = 11.84SRR31 pKa = 11.84SRR33 pKa = 11.84SGSKK37 pKa = 9.87SSSRR41 pKa = 11.84SRR43 pKa = 11.84SGSRR47 pKa = 11.84SSRR50 pKa = 11.84SRR52 pKa = 11.84SGSARR57 pKa = 11.84SRR59 pKa = 11.84SGSPQAISGGSPRR72 pKa = 11.84KK73 pKa = 9.44SRR75 pKa = 11.84SRR77 pKa = 11.84SSSRR81 pKa = 11.84RR82 pKa = 11.84SRR84 pKa = 11.84SGSASARR91 pKa = 11.84SRR93 pKa = 11.84SGSASVKK100 pKa = 9.91SRR102 pKa = 11.84SGSRR106 pKa = 11.84SRR108 pKa = 11.84SRR110 pKa = 11.84SRR112 pKa = 11.84SRR114 pKa = 11.84SGSVSPSRR122 pKa = 11.84ARR124 pKa = 11.84SRR126 pKa = 11.84SSNKK130 pKa = 9.98SVGKK134 pKa = 9.92SSKK137 pKa = 10.07NNSPARR143 pKa = 11.84SVSPANRR150 pKa = 11.84SGSVEE155 pKa = 4.0KK156 pKa = 10.21MDD158 pKa = 4.51VDD160 pKa = 3.84AEE162 pKa = 4.23EE163 pKa = 4.48NAPSRR168 pKa = 11.84TGSRR172 pKa = 11.84ASRR175 pKa = 11.84SRR177 pKa = 11.84SVSGSRR183 pKa = 11.84SRR185 pKa = 11.84SGSKK189 pKa = 10.38HH190 pKa = 5.67NSPEE194 pKa = 3.61DD195 pKa = 3.45ARR197 pKa = 11.84ASRR200 pKa = 11.84SRR202 pKa = 11.84SKK204 pKa = 10.66SGSRR208 pKa = 11.84SPVKK212 pKa = 10.12RR213 pKa = 11.84SKK215 pKa = 10.75SRR217 pKa = 11.84SKK219 pKa = 10.55SRR221 pKa = 11.84SRR223 pKa = 11.84SKK225 pKa = 10.6SRR227 pKa = 11.84SRR229 pKa = 11.84SKK231 pKa = 10.56SGSRR235 pKa = 11.84SKK237 pKa = 10.84SRR239 pKa = 11.84SRR241 pKa = 11.84SKK243 pKa = 10.56SGSRR247 pKa = 11.84SKK249 pKa = 10.84SRR251 pKa = 11.84SRR253 pKa = 11.84SKK255 pKa = 10.56SGSRR259 pKa = 11.84SKK261 pKa = 10.84SRR263 pKa = 11.84SRR265 pKa = 11.84SKK267 pKa = 10.6SRR269 pKa = 11.84SRR271 pKa = 11.84SKK273 pKa = 10.56SGSRR277 pKa = 11.84SKK279 pKa = 10.86SRR281 pKa = 11.84SKK283 pKa = 10.81SRR285 pKa = 11.84SKK287 pKa = 10.63SGSRR291 pKa = 11.84SKK293 pKa = 10.84SRR295 pKa = 11.84SRR297 pKa = 11.84SKK299 pKa = 10.6SRR301 pKa = 11.84SRR303 pKa = 11.84SKK305 pKa = 10.6SRR307 pKa = 11.84SRR309 pKa = 11.84SKK311 pKa = 10.6SRR313 pKa = 11.84SRR315 pKa = 11.84SKK317 pKa = 10.6SRR319 pKa = 11.84SRR321 pKa = 11.84SKK323 pKa = 10.56SGSRR327 pKa = 11.84SKK329 pKa = 10.84SRR331 pKa = 11.84SRR333 pKa = 11.84SKK335 pKa = 10.6SRR337 pKa = 11.84SRR339 pKa = 11.84SKK341 pKa = 10.6SRR343 pKa = 11.84SRR345 pKa = 11.84SKK347 pKa = 10.81SRR349 pKa = 11.84SKK351 pKa = 10.44SASRR355 pKa = 11.84AKK357 pKa = 10.38SGSRR361 pKa = 11.84SKK363 pKa = 10.89SKK365 pKa = 10.39SVSRR369 pKa = 11.84SRR371 pKa = 11.84SRR373 pKa = 11.84SRR375 pKa = 11.84SRR377 pKa = 11.84LKK379 pKa = 10.29SRR381 pKa = 11.84SRR383 pKa = 11.84VGSRR387 pKa = 11.84SGSAKK392 pKa = 9.64SVSRR396 pKa = 11.84SRR398 pKa = 11.84SGSRR402 pKa = 11.84AGSQSPARR410 pKa = 11.84KK411 pKa = 9.75GEE413 pKa = 3.44WRR415 pKa = 11.84AACRR419 pKa = 11.84TSRR422 pKa = 3.68

Molecular weight: 45.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15443

4419

19862

7582099

8

18544

381.7

42.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.519 ± 0.024

2.27 ± 0.088

5.526 ± 0.019

6.579 ± 0.032

3.611 ± 0.019

5.467 ± 0.028

2.468 ± 0.011

5.711 ± 0.019

6.459 ± 0.036

9.013 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.013

5.092 ± 0.025

5.25 ± 0.039

3.906 ± 0.018

5.621 ± 0.023

7.847 ± 0.023

5.721 ± 0.018

6.305 ± 0.017

1.111 ± 0.01

3.252 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski