Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Dinoroseobacter; Dinoroseobacter shibae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4086 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A8LLU7|RF1_DINSH Peptide chain release factor 1 OS=Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12) OX=398580 GN=prfA PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 10.1SSKK5 pKa = 9.94FSPVVALALPLALFGAPSLAGSLEE29 pKa = 4.26DD30 pKa = 3.71PVVEE34 pKa = 4.39PVPMAPAPVVVSGRR48 pKa = 11.84DD49 pKa = 3.14WTGGYY54 pKa = 9.86VGAQIGYY61 pKa = 10.54GDD63 pKa = 3.84VGTNASGVDD72 pKa = 3.54GDD74 pKa = 4.29GLIGGLIAGYY84 pKa = 10.37DD85 pKa = 3.91YY86 pKa = 11.73DD87 pKa = 3.83FGSYY91 pKa = 10.13VLGAGIDD98 pKa = 3.81YY99 pKa = 10.59DD100 pKa = 4.4IADD103 pKa = 4.28IDD105 pKa = 4.54LGPASLEE112 pKa = 3.83DD113 pKa = 3.67VLRR116 pKa = 11.84LKK118 pKa = 10.77LRR120 pKa = 11.84AGFEE124 pKa = 4.33AGPSLIYY131 pKa = 9.9ATGGYY136 pKa = 9.94ARR138 pKa = 11.84AGTDD142 pKa = 3.38NLGSDD147 pKa = 4.55DD148 pKa = 3.71GWFAGAGVEE157 pKa = 4.61YY158 pKa = 9.91PLSQNLSLAGEE169 pKa = 4.27VLYY172 pKa = 11.1HH173 pKa = 6.63EE174 pKa = 5.71FDD176 pKa = 4.39DD177 pKa = 4.79FDD179 pKa = 5.78GSGVDD184 pKa = 3.59VDD186 pKa = 3.75ATTVQARR193 pKa = 11.84LAYY196 pKa = 10.21RR197 pKa = 11.84FF198 pKa = 3.57

Molecular weight: 20.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8LMB7|A8LMB7_DINSH Uncharacterized protein OS=Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12) OX=398580 GN=Dshi_0345 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4086

0

4086

1291468

28

2553

316.1

34.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.082 ± 0.048

0.884 ± 0.012

5.958 ± 0.035

5.821 ± 0.036

3.709 ± 0.025

8.759 ± 0.042

1.95 ± 0.019

4.909 ± 0.03

2.736 ± 0.029

10.32 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.653 ± 0.019

2.322 ± 0.021

5.471 ± 0.031

3.049 ± 0.018

6.939 ± 0.045

4.878 ± 0.023

5.663 ± 0.028

7.373 ± 0.034

1.4 ± 0.015

2.12 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski