Arthrobacter alpinus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M4RPB4|A0A0M4RPB4_9MICC Nucleoside diphosphate kinase OS=Arthrobacter alpinus OX=656366 GN=ndk PE=3 SV=1
MM1 pKa = 7.86ADD3 pKa = 3.0SSTPAGVVVCQDD15 pKa = 2.77EE16 pKa = 4.31DD17 pKa = 4.19VQVKK21 pKa = 10.01SALLVEE27 pKa = 4.72VDD29 pKa = 4.68DD30 pKa = 5.02LPVAIVRR37 pKa = 11.84DD38 pKa = 3.83SHH40 pKa = 6.5GVLHH44 pKa = 7.41AIGDD48 pKa = 4.29TCSHH52 pKa = 6.73ADD54 pKa = 3.09ISLSEE59 pKa = 4.16GDD61 pKa = 3.83VEE63 pKa = 5.26EE64 pKa = 4.51GTIEE68 pKa = 4.15CWAHH72 pKa = 6.08GSSFDD77 pKa = 4.4LSTGEE82 pKa = 4.65PLTLPAFEE90 pKa = 4.63PVPVFALSVHH100 pKa = 5.41GTDD103 pKa = 3.84VYY105 pKa = 11.86VDD107 pKa = 3.18VTNILNGVSPQQ118 pKa = 3.16

Molecular weight: 12.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4RQP6|A0A0M4RQP6_9MICC MFS transporter OS=Arthrobacter alpinus OX=656366 GN=AOC05_13075 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.71GRR40 pKa = 11.84IEE42 pKa = 4.01LSAA45 pKa = 4.73

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3261

0

3261

1040438

37

2031

319.1

34.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.022 ± 0.056

0.64 ± 0.011

5.358 ± 0.032

5.368 ± 0.044

3.296 ± 0.026

8.913 ± 0.038

2.135 ± 0.022

4.64 ± 0.028

3.025 ± 0.03

10.356 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.019

2.675 ± 0.024

5.184 ± 0.032

3.199 ± 0.022

5.983 ± 0.044

5.973 ± 0.027

6.025 ± 0.035

8.519 ± 0.038

1.409 ± 0.017

2.049 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski